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The map label for this gene is hemL

Identifier: 121609968

GI number: 121609968

Start: 3377682

End: 3378995

Strand: Direct

Name: hemL

Synonym: Veis_3024

Alternate gene names: 121609968

Gene position: 3377682-3378995 (Clockwise)

Preceding gene: 121609967

Following gene: 121609973

Centisome position: 60.68

GC content: 67.5

Gene sequence:

>1314_bases
ATGACACCAAACACTGATCTGAATATCCCGCTGTTCGAGCGCGCCAAGGCGCTCATTCCCGGTGGCGTGAACTCGCCAGT
CCGGGCCTTCAAGGCAGTCGGCGGCACGCCCCGCTTCGTCAAGCGCGCCCGGGGCGCGTACTTCTGGGACGCCAACGACC
AGCGTTTCATCGACTACATCGGCTCCTGGGGACCGATGATTCTGGGGCATGGCCACCCGGCGGTGCTCGAAGCGGTGCAG
CAGGCAGCGCTGGAGGGTCTGAGCTTTGGCGCCCCCACCGAGCGCGAGTTGGAACTGGCCGAGGCCATCTTGCGCCTGCT
GCCATCGATGCAGATGATCCGCCTGGTCAGTTCGGGCACCGAGGCCGGCATGAGCGCGATCCGCCTGGCACGCGGCGCCA
CCGGCCGCAGCAAGCTCATCAAGTTCGAGGGCTGCTACCACGGCCATGCCGATGCGCTGCTGGTCAAGGCCGGTTCGGGC
CTGGCCACCTTCGGCAACGCCACCAGCGCCGGCGTGCCCGCCGAGGTGGTGCAGCACACGCTGGTGCTCGAATACAACAG
CATCGAGCAGCTCGAACGAGCCTTTGCATTGCACGGCAAGGAACTGGCCTGCCTGATCATCGAGCCGATCGCCGGCAACA
TGAACCTGGTGCGCGCCAGCGTGCCCTTCATGCGCCGCTGCCGCGAGTTGTGCAGCGAATACGGCGCATTGCTGGTGCTC
GACGAGGTGATGACCGGCTTTCGGGTGGCGCCGGGCGGCGCGCAAAGCCTGTACGCCCGGGACATACCAGGCTTCCAACC
CGATCTGACGGTGCTCGGCAAGGTGATTGGCGGCGGCATGCCGCTGGCCGCCTTTGGCGGGTCGCGCGCGCTGATGGAAC
ACCTGGCGCCATTGGGTGCGGTCTACCAGGCTGGCACCTTGTCGGGCAACCCGGTGGCCACGGCCTGCGGCCTGGCGACG
CTGCGCGCCATCGGCCAACCGGGCTTTTTTGCCGCGCTGTCGGCCCGCACCCGGGCGCTGGTCGAGGGGCTACGGGGCGC
TGCGGCGGCCGAAGGCGTGGCTTTCAGCGCGGACAGCGAAGGCGGCATGTTCGGCTTCTTTCTGCTGCCGGCGCTGCCCC
GGAACTACGCCCAGGTGCTGCAAACCGACAGCGCACGCTTCAACCAACTGTTCCACGGCCTGCTCGAACGCGGCATCTAC
ATCGCGCCGGCGCTCTACGAGGCCGGCTTTGTCAGCGCAGCGCACAGCGACGAGGACATTGCAGCCACCGTGGCTGCGGC
GCGCGAGGTGTTCAAGACGCTGGCAAAACCATAG

Upstream 100 bases:

>100_bases
TTCTGGGCCGCGCCCGGCGCCGACGATGCCCGGGACGCCGAAGAAGACAATCCCACCATTGACGATGCACAGGCCCTGGA
AGGCTTTGTGATGCCACCCC

Downstream 100 bases:

>100_bases
CGGCAGGGGCCATGCAATGGATGCGCCCGGGGCCGCCATCACCTCGATGCCGCGGATGCCCCTCAGTGGCGGAAATGCCG
CAGGCCGCTGAACACCATGG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTPNTDLNIPLFERAKALIPGGVNSPVRAFKAVGGTPRFVKRARGAYFWDANDQRFIDYIGSWGPMILGHGHPAVLEAVQ
QAALEGLSFGAPTERELELAEAILRLLPSMQMIRLVSSGTEAGMSAIRLARGATGRSKLIKFEGCYHGHADALLVKAGSG
LATFGNATSAGVPAEVVQHTLVLEYNSIEQLERAFALHGKELACLIIEPIAGNMNLVRASVPFMRRCRELCSEYGALLVL
DEVMTGFRVAPGGAQSLYARDIPGFQPDLTVLGKVIGGGMPLAAFGGSRALMEHLAPLGAVYQAGTLSGNPVATACGLAT
LRAIGQPGFFAALSARTRALVEGLRGAAAAEGVAFSADSEGGMFGFFLLPALPRNYAQVLQTDSARFNQLFHGLLERGIY
IAPALYEAGFVSAAHSDEDIAATVAAAREVFKTLAKP

Sequences:

>Translated_437_residues
MTPNTDLNIPLFERAKALIPGGVNSPVRAFKAVGGTPRFVKRARGAYFWDANDQRFIDYIGSWGPMILGHGHPAVLEAVQ
QAALEGLSFGAPTERELELAEAILRLLPSMQMIRLVSSGTEAGMSAIRLARGATGRSKLIKFEGCYHGHADALLVKAGSG
LATFGNATSAGVPAEVVQHTLVLEYNSIEQLERAFALHGKELACLIIEPIAGNMNLVRASVPFMRRCRELCSEYGALLVL
DEVMTGFRVAPGGAQSLYARDIPGFQPDLTVLGKVIGGGMPLAAFGGSRALMEHLAPLGAVYQAGTLSGNPVATACGLAT
LRAIGQPGFFAALSARTRALVEGLRGAAAAEGVAFSADSEGGMFGFFLLPALPRNYAQVLQTDSARFNQLFHGLLERGIY
IAPALYEAGFVSAAHSDEDIAATVAAAREVFKTLAKP
>Mature_436_residues
TPNTDLNIPLFERAKALIPGGVNSPVRAFKAVGGTPRFVKRARGAYFWDANDQRFIDYIGSWGPMILGHGHPAVLEAVQQ
AALEGLSFGAPTERELELAEAILRLLPSMQMIRLVSSGTEAGMSAIRLARGATGRSKLIKFEGCYHGHADALLVKAGSGL
ATFGNATSAGVPAEVVQHTLVLEYNSIEQLERAFALHGKELACLIIEPIAGNMNLVRASVPFMRRCRELCSEYGALLVLD
EVMTGFRVAPGGAQSLYARDIPGFQPDLTVLGKVIGGGMPLAAFGGSRALMEHLAPLGAVYQAGTLSGNPVATACGLATL
RAIGQPGFFAALSARTRALVEGLRGAAAAEGVAFSADSEGGMFGFFLLPALPRNYAQVLQTDSARFNQLFHGLLERGIYI
APALYEAGFVSAAHSDEDIAATVAAAREVFKTLAKP

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=296, Percent_Identity=26.3513513513513, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=55.7647058823529, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1789016, Length=354, Percent_Identity=31.9209039548023, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787560, Length=288, Percent_Identity=29.8611111111111, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1788044, Length=313, Percent_Identity=30.6709265175719, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1789759, Length=312, Percent_Identity=25.9615384615385, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI145693181, Length=313, Percent_Identity=28.7539936102236, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786991, Length=294, Percent_Identity=27.2108843537415, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25144271, Length=301, Percent_Identity=26.578073089701, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323470, Length=340, Percent_Identity=24.1176470588235, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324432, Length=328, Percent_Identity=25.3048780487805, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21357415, Length=298, Percent_Identity=25.503355704698, Blast_Score=96, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_VEREI (A1WMA0)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_997775.1
- ProteinModelPortal:   A1WMA0
- SMR:   A1WMA0
- STRING:   A1WMA0
- GeneID:   4694901
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_3024
- NMPDR:   fig|391735.5.peg.2809
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- PhylomeDB:   A1WMA0
- ProtClustDB:   PRK00062
- BioCyc:   VEIS391735:VEIS_3024-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46111; Mature: 45980

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPNTDLNIPLFERAKALIPGGVNSPVRAFKAVGGTPRFVKRARGAYFWDANDQRFIDYI
CCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCEEECCCCCHHHHHH
GSWGPMILGHGHPAVLEAVQQAALEGLSFGAPTERELELAEAILRLLPSMQMIRLVSSGT
CCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCC
EAGMSAIRLARGATGRSKLIKFEGCYHGHADALLVKAGSGLATFGNATSAGVPAEVVQHT
HHHHHHHHHHHCCCCCHHEEEEECCCCCCCCEEEEECCCCCEECCCCCCCCCCHHHHHHH
LVLEYNSIEQLERAFALHGKELACLIIEPIAGNMNLVRASVPFMRRCRELCSEYGALLVL
HHEECCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHCCCEEEH
DEVMTGFRVAPGGAQSLYARDIPGFQPDLTVLGKVIGGGMPLAAFGGSRALMEHLAPLGA
HHHHHCCEECCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHH
VYQAGTLSGNPVATACGLATLRAIGQPGFFAALSARTRALVEGLRGAAAAEGVAFSADSE
HHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
GGMFGFFLLPALPRNYAQVLQTDSARFNQLFHGLLERGIYIAPALYEAGFVSAAHSDEDI
CCEEHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCHHH
AATVAAAREVFKTLAKP
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPNTDLNIPLFERAKALIPGGVNSPVRAFKAVGGTPRFVKRARGAYFWDANDQRFIDYI
CCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCEEECCCCCHHHHHH
GSWGPMILGHGHPAVLEAVQQAALEGLSFGAPTERELELAEAILRLLPSMQMIRLVSSGT
CCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCC
EAGMSAIRLARGATGRSKLIKFEGCYHGHADALLVKAGSGLATFGNATSAGVPAEVVQHT
HHHHHHHHHHHCCCCCHHEEEEECCCCCCCCEEEEECCCCCEECCCCCCCCCCHHHHHHH
LVLEYNSIEQLERAFALHGKELACLIIEPIAGNMNLVRASVPFMRRCRELCSEYGALLVL
HHEECCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHCCCEEEH
DEVMTGFRVAPGGAQSLYARDIPGFQPDLTVLGKVIGGGMPLAAFGGSRALMEHLAPLGA
HHHHHCCEECCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHH
VYQAGTLSGNPVATACGLATLRAIGQPGFFAALSARTRALVEGLRGAAAAEGVAFSADSE
HHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
GGMFGFFLLPALPRNYAQVLQTDSARFNQLFHGLLERGIYIAPALYEAGFVSAAHSDEDI
CCEEHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCHHH
AATVAAAREVFKTLAKP
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA