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The map label for this gene is rluF [H]

Identifier: 120612927

GI number: 120612927

Start: 4762544

End: 4763338

Strand: Reverse

Name: rluF [H]

Synonym: Aave_4291

Alternate gene names: 120612927

Gene position: 4763338-4762544 (Counterclockwise)

Preceding gene: 120612928

Following gene: 120612925

Centisome position: 88.99

GC content: 72.2

Gene sequence:

>795_bases
ATGGGCGCGCCCGCCGGGGGGCACCGCGGTGACGCGCCGTCCGGCACCTCGCGCCTGAACAAGCGCATGGCCGAACTGGG
CCTGTGCTCGCGCCGCGAGGCGGACGACTGGATCGCCAGCGGCTGGGTGCGCGTGAACGGCCAGGTGGCCGCCATGGGGC
TGCAGGTTGCGCCCACGGACCGCATCGAGGTGGACCCCGCGGCCCGCGGCCAGCAGGAACGGCAGGCGACCATCCTGCTG
CACAAGCCCATGGGCTATGTCAGCGGGCAGGCCGAGGACGGCCACACGCCGGCGGTCGCGCTCATCAACCCGCGCACCCA
CTGGCGCGAGGACCCGAGCCGGCAGCGCTTCTCGCCCCCGCAACTGCGCGGCCTCGCGCCCTGCGGCCGGCTGGACATCG
ACTCCGTCGGGCTGCTGGTGCTCACGCAGGACGGGCGCGTGGCGCGGCATCTGATCGGCGAAGACTCCGAAGTCGAGAAG
GAATACCTCGTGCGCGTGCACTACGGCGACATCGCGACGGACGTGCAGTCCGCCTTCCCGGCCGGGCAGCTGGCGCGCCT
GTGCCACGGTCTGTCGCTGGACGGCCAGGCGCTCAAGCCGGCGAAGGTGGAGTGGCAGAACCCCGAGCAGCTGCGCTTCG
TGCTCAAGGAGGGCAAGAAGCGCCAGATCCGCCGCATGTGCGAACTGGTGGGCCTGAAGGTGGTGGGCCTCAAGCGCATC
CGCATCGGCCGCGTGGTGCTGGGCAACCTGCCCGTGGGGCAGTGGCGCTACCTGGGGCCGAACGAGCGGTTCTGA

Upstream 100 bases:

>100_bases
CCCGGAGGCCGGTGCAGCCGCTGCACGCACCGCTCCCGCCGGCCGGGAGGCTCGTGCGCCCTCGCGCGCTGGCCCCGGTG
CCGCGCGCACTTCCGCACCC

Downstream 100 bases:

>100_bases
TCAGCCGCCGCCCGCAGCGGCCGCGGCCATCGGGCAGGAAGTGGGGCTCGGCAGCAGTCATGTCAACGAATTCAATCGCG
CCCCTGCCGTCCTGCCAAAT

Product: RNA-binding S4 domain-containing protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MGAPAGGHRGDAPSGTSRLNKRMAELGLCSRREADDWIASGWVRVNGQVAAMGLQVAPTDRIEVDPAARGQQERQATILL
HKPMGYVSGQAEDGHTPAVALINPRTHWREDPSRQRFSPPQLRGLAPCGRLDIDSVGLLVLTQDGRVARHLIGEDSEVEK
EYLVRVHYGDIATDVQSAFPAGQLARLCHGLSLDGQALKPAKVEWQNPEQLRFVLKEGKKRQIRRMCELVGLKVVGLKRI
RIGRVVLGNLPVGQWRYLGPNERF

Sequences:

>Translated_264_residues
MGAPAGGHRGDAPSGTSRLNKRMAELGLCSRREADDWIASGWVRVNGQVAAMGLQVAPTDRIEVDPAARGQQERQATILL
HKPMGYVSGQAEDGHTPAVALINPRTHWREDPSRQRFSPPQLRGLAPCGRLDIDSVGLLVLTQDGRVARHLIGEDSEVEK
EYLVRVHYGDIATDVQSAFPAGQLARLCHGLSLDGQALKPAKVEWQNPEQLRFVLKEGKKRQIRRMCELVGLKVVGLKRI
RIGRVVLGNLPVGQWRYLGPNERF
>Mature_263_residues
GAPAGGHRGDAPSGTSRLNKRMAELGLCSRREADDWIASGWVRVNGQVAAMGLQVAPTDRIEVDPAARGQQERQATILLH
KPMGYVSGQAEDGHTPAVALINPRTHWREDPSRQRFSPPQLRGLAPCGRLDIDSVGLLVLTQDGRVARHLIGEDSEVEKE
YLVRVHYGDIATDVQSAFPAGQLARLCHGLSLDGQALKPAKVEWQNPEQLRFVLKEGKKRQIRRMCELVGLKVVGLKRIR
IGRVVLGNLPVGQWRYLGPNERF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=252, Percent_Identity=36.1111111111111, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1788510, Length=247, Percent_Identity=29.1497975708502, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787524, Length=261, Percent_Identity=32.183908045977, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 29147; Mature: 29016

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAPAGGHRGDAPSGTSRLNKRMAELGLCSRREADDWIASGWVRVNGQVAAMGLQVAPTD
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEECCEEEEEEEEECCCC
RIEVDPAARGQQERQATILLHKPMGYVSGQAEDGHTPAVALINPRTHWREDPSRQRFSPP
CEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHCCCCCCCCCCCCH
QLRGLAPCGRLDIDSVGLLVLTQDGRVARHLIGEDSEVEKEYLVRVHYGDIATDVQSAFP
HHCCCCCCCCCCCCCCCEEEEECCCHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHCC
AGQLARLCHGLSLDGQALKPAKVEWQNPEQLRFVLKEGKKRQIRRMCELVGLKVVGLKRI
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEE
RIGRVVLGNLPVGQWRYLGPNERF
EECEEEECCCCCCCEEECCCCCCC
>Mature Secondary Structure 
GAPAGGHRGDAPSGTSRLNKRMAELGLCSRREADDWIASGWVRVNGQVAAMGLQVAPTD
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEECCEEEEEEEEECCCC
RIEVDPAARGQQERQATILLHKPMGYVSGQAEDGHTPAVALINPRTHWREDPSRQRFSPP
CEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHCCCCCCCCCCCCH
QLRGLAPCGRLDIDSVGLLVLTQDGRVARHLIGEDSEVEKEYLVRVHYGDIATDVQSAFP
HHCCCCCCCCCCCCCCCEEEEECCCHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHCC
AGQLARLCHGLSLDGQALKPAKVEWQNPEQLRFVLKEGKKRQIRRMCELVGLKVVGLKRI
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEE
RIGRVVLGNLPVGQWRYLGPNERF
EECEEEECCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]