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The map label for this gene is gloA [H]

Identifier: 120612607

GI number: 120612607

Start: 4397848

End: 4398297

Strand: Direct

Name: gloA [H]

Synonym: Aave_3968

Alternate gene names: 120612607

Gene position: 4397848-4398297 (Clockwise)

Preceding gene: 120612606

Following gene: 120612609

Centisome position: 82.16

GC content: 59.56

Gene sequence:

>450_bases
TTGACGATCTGCCAGCGTGACTCCCAGTCGAGCCGGCCCCCTTGTCGGATCCTAGAAATGGCCAAGATCATCCACACCAT
GGTGCGCGTGCACGACCTCGAAAAGTCGCTGCAGTTCTACATGGCGGCACTCCGCCTCACCACGAGCCATCGGCTCGATT
TCGATGATTTTTCTCTGGTGTACCTGCGAAATCCGGAGAACGATGTCGAGATCGAGCTGACGTGGAACAAGGGGCGGGAG
CCCTACACCCACGGCGACGGCTACGGGCATATCGCTGTCGCAGTCGAAAACCTGGAAGCCGAGCACGAGCGGATGACCGG
CGCCGGCATGCACCCGACGCCGGTGAAGGAGTTCAAGCGAGGCCAGGACATACTGGCCCGCTTTTTCTTCATTCAGGACC
CCGACGGGTACAAGATCGAAGTGCTCGAACGCTCAGGACACTACGTCTGA

Upstream 100 bases:

>100_bases
GACGGAAAGATCGCAGTCCAGACCTTCACCGCGTTCTCGGCGTAGCGGGACAGTCGCGGAAGACATCCTCCAGTTCACCG
TCGACGGGTCCTCACCGCCG

Downstream 100 bases:

>100_bases
ACTGCGACGCCGTGTATAGGCCCCCGCGGCCTCCCGTCGAACCTCGCCGGGGGAGCTGCCGTACTTCTCCTTGAAGGTAC
GGCAAAAATGGCTCATCGAG

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MTICQRDSQSSRPPCRILEMAKIIHTMVRVHDLEKSLQFYMAALRLTTSHRLDFDDFSLVYLRNPENDVEIELTWNKGRE
PYTHGDGYGHIAVAVENLEAEHERMTGAGMHPTPVKEFKRGQDILARFFFIQDPDGYKIEVLERSGHYV

Sequences:

>Translated_149_residues
MTICQRDSQSSRPPCRILEMAKIIHTMVRVHDLEKSLQFYMAALRLTTSHRLDFDDFSLVYLRNPENDVEIELTWNKGRE
PYTHGDGYGHIAVAVENLEAEHERMTGAGMHPTPVKEFKRGQDILARFFFIQDPDGYKIEVLERSGHYV
>Mature_148_residues
TICQRDSQSSRPPCRILEMAKIIHTMVRVHDLEKSLQFYMAALRLTTSHRLDFDDFSLVYLRNPENDVEIELTWNKGREP
YTHGDGYGHIAVAVENLEAEHERMTGAGMHPTPVKEFKRGQDILARFFFIQDPDGYKIEVLERSGHYV

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Escherichia coli, GI1787940, Length=128, Percent_Identity=38.28125, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 17323; Mature: 17192

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTICQRDSQSSRPPCRILEMAKIIHTMVRVHDLEKSLQFYMAALRLTTSHRLDFDDFSLV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
YLRNPENDVEIELTWNKGREPYTHGDGYGHIAVAVENLEAEHERMTGAGMHPTPVKEFKR
EEECCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECHHHHHHHHCCCCCCCCCHHHHHC
GQDILARFFFIQDPDGYKIEVLERSGHYV
CHHHHHHEEEEECCCCCEEEEEECCCCCC
>Mature Secondary Structure 
TICQRDSQSSRPPCRILEMAKIIHTMVRVHDLEKSLQFYMAALRLTTSHRLDFDDFSLV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
YLRNPENDVEIELTWNKGREPYTHGDGYGHIAVAVENLEAEHERMTGAGMHPTPVKEFKR
EEECCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECHHHHHHHHCCCCCCCCCHHHHHC
GQDILARFFFIQDPDGYKIEVLERSGHYV
CHHHHHHEEEEECCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 9719565 [H]