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The map label for this gene is pucA [H]

Identifier: 120612572

GI number: 120612572

Start: 4359547

End: 4360530

Strand: Direct

Name: pucA [H]

Synonym: Aave_3933

Alternate gene names: 120612572

Gene position: 4359547-4360530 (Clockwise)

Preceding gene: 120612571

Following gene: 120612573

Centisome position: 81.44

GC content: 73.78

Gene sequence:

>984_bases
ATGGAGGCGACGGACCTGGACAGCCTCGTGCTGCGCACGGCGAACGGCTGGCTGCAGGCCGGCGGGCGCGCGTGGCTGGT
GACCGTCGCACGCACCTGGGGATCGTCCCCGCGGCCTCCCGGGTCGCTCATGGCGATGAATGCGCGCGGCGAGGTGGTGG
GCTCCGTCTCGGGCGGCTGCATCGAAGACGATCTCGTGCGCCGCGTGCAGCGGCAGGCCACCGCGCAGGCGCAGGCCGAG
GGCGCCACGGCGCTCCCCGATGTCCTGCGCTACGGCATCTCGGCCGACGAGGCCCACCGCTTCGGCCTGCCCTGCGGCGG
CACCGTGGAACTGGTGATGGAGCCGCTGCACGGACGCAGCCGGCTGCAGGAACTGCTGGACGGCTGTGCGCAGCGGCGCT
GCGTGGAGCGCGCGCTGCACCTGGCCAGCGGCGAAGTGCTGCTGCGCGATGTCGCGCACGGCGCGGCGCCGCTGCTGGAC
GCGGAGCGCTTCGTGACACGCTTCGCGCCGGTCGCGCGCCTGGTCGTCATCGGCGCGGGCGACACCGCGTATTACGCGTG
CCAGATGGCGGTGAACGTCGGCTTCGACATCGTGCTGTGCGACCCGCGCCTGGAGCAGGCCCCGGCGTGGGCGCGGGGCC
GCATCGCCTTCACGCGCGACATGCCCGACGACGTGGTCCTGGCGCTGCGGGACGACGACCGCACGGCCGTGCTCGCCCTG
AGCCACGACCCCAAGCTGGACGACCTGGCGCTGATCGAGGCCCTGCGTTCCCGGGCCTTCTACGTCGGCGCCATCGGCTC
GCGCAACAACAGCGCGCGGCGGCGCGAGCGGCTGCAGGCGCACTTCGGGCTTCAGGACTCCGAACTGAAGCGCCTGCACG
GCCCCGCGGGCCTGTTCATCGGCAGCCGGACGCCGGCCGAGATCGCCCTCTCGATGGTGGCCGAGTTGGTGGCCGCGAAG
AACGGCGTGCCTGCGGGCGCGTGA

Upstream 100 bases:

>100_bases
GCCGCGCTGGCCAATGCGCTGTTCGACGCCACGGGGCGGCGCTTCTACCGGGCGCCGTTCACCCCGGACGCCGTGCGGTC
CGCACTCGGCAGTGCCTGAC

Downstream 100 bases:

>100_bases
TTTCCGGCGCTGCCCCTGTACTACCCCCGGCACTCCACGGCAGTGGATCGCGGCACCGGCCTACATCGGCACCTTCCCGT
TCTTATTGACAACATATTGT

Product: hypothetical protein

Products: NA

Alternate protein names: XDHase subunit A [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MEATDLDSLVLRTANGWLQAGGRAWLVTVARTWGSSPRPPGSLMAMNARGEVVGSVSGGCIEDDLVRRVQRQATAQAQAE
GATALPDVLRYGISADEAHRFGLPCGGTVELVMEPLHGRSRLQELLDGCAQRRCVERALHLASGEVLLRDVAHGAAPLLD
AERFVTRFAPVARLVVIGAGDTAYYACQMAVNVGFDIVLCDPRLEQAPAWARGRIAFTRDMPDDVVLALRDDDRTAVLAL
SHDPKLDDLALIEALRSRAFYVGAIGSRNNSARRRERLQAHFGLQDSELKRLHGPAGLFIGSRTPAEIALSMVAELVAAK
NGVPAGA

Sequences:

>Translated_327_residues
MEATDLDSLVLRTANGWLQAGGRAWLVTVARTWGSSPRPPGSLMAMNARGEVVGSVSGGCIEDDLVRRVQRQATAQAQAE
GATALPDVLRYGISADEAHRFGLPCGGTVELVMEPLHGRSRLQELLDGCAQRRCVERALHLASGEVLLRDVAHGAAPLLD
AERFVTRFAPVARLVVIGAGDTAYYACQMAVNVGFDIVLCDPRLEQAPAWARGRIAFTRDMPDDVVLALRDDDRTAVLAL
SHDPKLDDLALIEALRSRAFYVGAIGSRNNSARRRERLQAHFGLQDSELKRLHGPAGLFIGSRTPAEIALSMVAELVAAK
NGVPAGA
>Mature_327_residues
MEATDLDSLVLRTANGWLQAGGRAWLVTVARTWGSSPRPPGSLMAMNARGEVVGSVSGGCIEDDLVRRVQRQATAQAQAE
GATALPDVLRYGISADEAHRFGLPCGGTVELVMEPLHGRSRLQELLDGCAQRRCVERALHLASGEVLLRDVAHGAAPLLD
AERFVTRFAPVARLVVIGAGDTAYYACQMAVNVGFDIVLCDPRLEQAPAWARGRIAFTRDMPDDVVLALRDDDRTAVLAL
SHDPKLDDLALIEALRSRAFYVGAIGSRNNSARRRERLQAHFGLQDSELKRLHGPAGLFIGSRTPAEIALSMVAELVAAK
NGVPAGA

Specific function: Oxidizes hypoxanthine and xanthine to uric acid. PucA subunit could exert a molybdenum cofactor recruiting function [H]

COG id: COG1975

COG function: function code O; Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786477, Length=317, Percent_Identity=26.4984227129338, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789240, Length=177, Percent_Identity=30.5084745762712, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003777 [H]

Pfam domain/function: PF02625 XdhC_CoxI [H]

EC number: =1.17.1.4 [H]

Molecular weight: Translated: 35072; Mature: 35072

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEATDLDSLVLRTANGWLQAGGRAWLVTVARTWGSSPRPPGSLMAMNARGEVVGSVSGGC
CCCCCHHHHHHHHCCCHHHCCCCEEEEEEEHHCCCCCCCCCCEEEECCCCCEEECCCCCC
IEDDLVRRVQRQATAQAQAEGATALPDVLRYGISADEAHRFGLPCGGTVELVMEPLHGRS
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHCCCCHH
RLQELLDGCAQRRCVERALHLASGEVLLRDVAHGAAPLLDAERFVTRFAPVARLVVIGAG
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEEECC
DTAYYACQMAVNVGFDIVLCDPRLEQAPAWARGRIAFTRDMPDDVVLALRDDDRTAVLAL
CHHHHEEEEEEECCEEEEEECCCCHHCCCCCCCCEEEECCCCCCEEEEEECCCCEEEEEE
SHDPKLDDLALIEALRSRAFYVGAIGSRNNSARRRERLQAHFGLQDSELKRLHGPAGLFI
CCCCCCHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCEEEE
GSRTPAEIALSMVAELVAAKNGVPAGA
CCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEATDLDSLVLRTANGWLQAGGRAWLVTVARTWGSSPRPPGSLMAMNARGEVVGSVSGGC
CCCCCHHHHHHHHCCCHHHCCCCEEEEEEEHHCCCCCCCCCCEEEECCCCCEEECCCCCC
IEDDLVRRVQRQATAQAQAEGATALPDVLRYGISADEAHRFGLPCGGTVELVMEPLHGRS
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHCCCCHH
RLQELLDGCAQRRCVERALHLASGEVLLRDVAHGAAPLLDAERFVTRFAPVARLVVIGAG
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEEECC
DTAYYACQMAVNVGFDIVLCDPRLEQAPAWARGRIAFTRDMPDDVVLALRDDDRTAVLAL
CHHHHEEEEEEECCEEEEEECCCCHHCCCCCCCCEEEECCCCCCEEEEEECCCCEEEEEE
SHDPKLDDLALIEALRSRAFYVGAIGSRNNSARRRERLQAHFGLQDSELKRLHGPAGLFI
CCCCCCHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCEEEE
GSRTPAEIALSMVAELVAAKNGVPAGA
CCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]