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The map label for this gene is poxB [H]

Identifier: 120612474

GI number: 120612474

Start: 4260337

End: 4262088

Strand: Direct

Name: poxB [H]

Synonym: Aave_3833

Alternate gene names: 120612474

Gene position: 4260337-4262088 (Clockwise)

Preceding gene: 120612473

Following gene: 120612475

Centisome position: 79.59

GC content: 70.49

Gene sequence:

>1752_bases
ATGGGCATTTTTGGTTCCTCCAAGGCGAAATCGGTGGCCGACCTGCTGGTCGAGACGCTGGCGCATGCCGGCGTGCAACG
CATCTGGGGCGTCACCGGCGACAGCCTGAACGCCATCAACGACAGCCTGCGCCGGCACGGCGGCATCGAATGGATGCACG
TGCGCCACGAAGAGGCCGGCGCCTTCGCCGCGGGCGCCGAGGCCCAGGTCACGGGGCGGCTCGCCGTGTGCGCGGGCAGC
TGCGGTCCGGGCAACCTCCACCTCATCAACGGGCTCTACGACTGCCAGCGCAACCACCAGCCCGTGCTGGCCATCGCCAG
CCACATCCCCTCCAGCGAGATCGGCCTGGGCTACTTCCAGGAAACCCACCCGACCGAGCTGTTCCGCGAATGCAGCCATT
TCTGCGAGATGGTGCAGGATCCGAAGCAGTTGCCCGAGGTGCTGCACCGCGCGATGCGCACCGCCATAGGCCGCCACGGC
GTGGCCGTGATCGTGCTGCCGGGCGACGTGTCCACCCTCGAAGCGCCGGAGGGCGCGCGGCCCGATTTCGCACCGCCGGC
CCTGCCGCGCCTGCTGCCCGACGCGGCCGCGCTCGCGGACCTCGCCACCCTGCTGAACGGCTCGGAGGCCGTGACCATCC
TGGCCGGCGCCGGCACGGCCGGTGCGCACGACGAGGTGGTGGCCCTGGCGAAGGCACTGGCCGCGCCCATCGTGCACGCC
TTCCGCGGCAAGGAGCACATGGAGTGGGACAACCCGTTCGACGTCGGCATGACCGGCCTGATCGGCTTCTCGTCCGGCTA
CCACGCGATGCGCGAGTGCGACACGCTGCTCATGCTGGGCACGGACTTTCCGTACCGCGACTTCTATCCGGAAGATGCGA
CCATCGTGCAGATCGACCGCGACCCCGGCGCGCTGGGCCGGCGCGCGCAATTGCGCATGGGTCTGGTCGCCGACGTGCGC
GAAGCCGCCGCCATGCTGGCTCCGCTCATCCAGCCGGGCCGCGACACCGGCTTTCTCGACAAGGCGCGCGAGCACTACCA
GGCGGCGCGCAGGGACCTGGACGGACTCGCGTCGCCGCGCGCCGGCGACGAGCCGCTGCACCCGCAGTTCGTGGCGGCCA
CGCTCGACCGGCTGGCTGCCGACGATGCGGTCTTCACGTTCGACGTGGGCACGCCGGCCATCTGGGCCGCGCGCTACCTG
CGCATGAACGGCCGGCGGCGGCTGCTCGGCTCCTTCAACCACGGCTCGATGGCCAACGCGATGCCGCAGGCGCTGGGCGC
GCAGGCGGTGCGGCCCGACCGCCAGGTGGTGTCGATCTCCGGCGACGGCGGCCTCGCGATGCTGATGGGCGACATGCTGA
CCGCCGTGCAGATGAAGCTGCCGATCAAGATCGTGCTCATCAACAACGGCGTGCTCGGCTTCGTGCAGATGGAGCAGAAA
GCCGCAGGCTACCTGGACACCAACGTCGCGCTGCAGAACCCGGATTTCAGCGCCATCGCGAACGGCATGGGCTTCCTGGG
CCTGCGCGTCGCCCGCTCGGACGAACTCGAGCCCGCCCTGGTGCGGGCGCTGGCCCACGACGGCCCCGCCCTGGTGGACG
TGCGGGTGGATCCGATGGAGCTGTCGATGCCGCCGAAGGTCAAGGCCGCGCAGGTGAAGGGCTTCAGCCTGTATGCCGCG
CGCGCGGTGATGAACGGCCGGGGCGACGAGATCGTCGAGCTGGCGAAAACCAACCTGCTGCGCCGGAGGTGA

Upstream 100 bases:

>100_bases
CTCTCCGCTGAAAGCGAAAACGTCCGACACCCAGGGTTTCCACCGATGGCACTGCGGTACCTAGAATGATCCGTCCACAA
CTTCCGGGGAGTTCCTGGCG

Downstream 100 bases:

>100_bases
CGGCGGCCGGCGCGGCGCCGGACCGGGGAGGCTCTTAAAATCATCCGTTGCGCCCGGAGCCCTCCGCCCGGGCCGCACTC
CCCTCAGAAAGCCCGCTCCC

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MGIFGSSKAKSVADLLVETLAHAGVQRIWGVTGDSLNAINDSLRRHGGIEWMHVRHEEAGAFAAGAEAQVTGRLAVCAGS
CGPGNLHLINGLYDCQRNHQPVLAIASHIPSSEIGLGYFQETHPTELFRECSHFCEMVQDPKQLPEVLHRAMRTAIGRHG
VAVIVLPGDVSTLEAPEGARPDFAPPALPRLLPDAAALADLATLLNGSEAVTILAGAGTAGAHDEVVALAKALAAPIVHA
FRGKEHMEWDNPFDVGMTGLIGFSSGYHAMRECDTLLMLGTDFPYRDFYPEDATIVQIDRDPGALGRRAQLRMGLVADVR
EAAAMLAPLIQPGRDTGFLDKAREHYQAARRDLDGLASPRAGDEPLHPQFVAATLDRLAADDAVFTFDVGTPAIWAARYL
RMNGRRRLLGSFNHGSMANAMPQALGAQAVRPDRQVVSISGDGGLAMLMGDMLTAVQMKLPIKIVLINNGVLGFVQMEQK
AAGYLDTNVALQNPDFSAIANGMGFLGLRVARSDELEPALVRALAHDGPALVDVRVDPMELSMPPKVKAAQVKGFSLYAA
RAVMNGRGDEIVELAKTNLLRRR

Sequences:

>Translated_583_residues
MGIFGSSKAKSVADLLVETLAHAGVQRIWGVTGDSLNAINDSLRRHGGIEWMHVRHEEAGAFAAGAEAQVTGRLAVCAGS
CGPGNLHLINGLYDCQRNHQPVLAIASHIPSSEIGLGYFQETHPTELFRECSHFCEMVQDPKQLPEVLHRAMRTAIGRHG
VAVIVLPGDVSTLEAPEGARPDFAPPALPRLLPDAAALADLATLLNGSEAVTILAGAGTAGAHDEVVALAKALAAPIVHA
FRGKEHMEWDNPFDVGMTGLIGFSSGYHAMRECDTLLMLGTDFPYRDFYPEDATIVQIDRDPGALGRRAQLRMGLVADVR
EAAAMLAPLIQPGRDTGFLDKAREHYQAARRDLDGLASPRAGDEPLHPQFVAATLDRLAADDAVFTFDVGTPAIWAARYL
RMNGRRRLLGSFNHGSMANAMPQALGAQAVRPDRQVVSISGDGGLAMLMGDMLTAVQMKLPIKIVLINNGVLGFVQMEQK
AAGYLDTNVALQNPDFSAIANGMGFLGLRVARSDELEPALVRALAHDGPALVDVRVDPMELSMPPKVKAAQVKGFSLYAA
RAVMNGRGDEIVELAKTNLLRRR
>Mature_582_residues
GIFGSSKAKSVADLLVETLAHAGVQRIWGVTGDSLNAINDSLRRHGGIEWMHVRHEEAGAFAAGAEAQVTGRLAVCAGSC
GPGNLHLINGLYDCQRNHQPVLAIASHIPSSEIGLGYFQETHPTELFRECSHFCEMVQDPKQLPEVLHRAMRTAIGRHGV
AVIVLPGDVSTLEAPEGARPDFAPPALPRLLPDAAALADLATLLNGSEAVTILAGAGTAGAHDEVVALAKALAAPIVHAF
RGKEHMEWDNPFDVGMTGLIGFSSGYHAMRECDTLLMLGTDFPYRDFYPEDATIVQIDRDPGALGRRAQLRMGLVADVRE
AAAMLAPLIQPGRDTGFLDKAREHYQAARRDLDGLASPRAGDEPLHPQFVAATLDRLAADDAVFTFDVGTPAIWAARYLR
MNGRRRLLGSFNHGSMANAMPQALGAQAVRPDRQVVSISGDGGLAMLMGDMLTAVQMKLPIKIVLINNGVLGFVQMEQKA
AGYLDTNVALQNPDFSAIANGMGFLGLRVARSDELEPALVRALAHDGPALVDVRVDPMELSMPPKVKAAQVKGFSLYAAR
AVMNGRGDEIVELAKTNLLRRR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=22.7112676056338, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1787096, Length=572, Percent_Identity=60.4895104895105, Blast_Score=707, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=559, Percent_Identity=29.1592128801431, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=28.4697508896797, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1786717, Length=553, Percent_Identity=26.9439421338156, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1788716, Length=549, Percent_Identity=23.3151183970856, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17531299, Length=564, Percent_Identity=25.531914893617, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17531301, Length=564, Percent_Identity=25.531914893617, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323755, Length=558, Percent_Identity=24.3727598566308, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320816, Length=551, Percent_Identity=23.049001814882, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922626, Length=490, Percent_Identity=25.5102040816327, Blast_Score=113, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 62437; Mature: 62306

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFGSSKAKSVADLLVETLAHAGVQRIWGVTGDSLNAINDSLRRHGGIEWMHVRHEEAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCC
AFAAGAEAQVTGRLAVCAGSCGPGNLHLINGLYDCQRNHQPVLAIASHIPSSEIGLGYFQ
CEECCCCCEEECEEEEEECCCCCCCEEEECCHHHHCCCCCCHHHHHHHCCCCCCCCCCCC
ETHPTELFRECSHFCEMVQDPKQLPEVLHRAMRTAIGRHGVAVIVLPGDVSTLEAPEGAR
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
PDFAPPALPRLLPDAAALADLATLLNGSEAVTILAGAGTAGAHDEVVALAKALAAPIVHA
CCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
FRGKEHMEWDNPFDVGMTGLIGFSSGYHAMRECDTLLMLGTDFPYRDFYPEDATIVQIDR
HCCCCCCCCCCCCCCCCHHEEECCCCHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEEC
DPGALGRRAQLRMGLVADVREAAAMLAPLIQPGRDTGFLDKAREHYQAARRDLDGLASPR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AGDEPLHPQFVAATLDRLAADDAVFTFDVGTPAIWAARYLRMNGRRRLLGSFNHGSMANA
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCHHEEECCCCCCHHHH
MPQALGAQAVRPDRQVVSISGDGGLAMLMGDMLTAVQMKLPIKIVLINNGVLGFVQMEQK
HHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEHHH
AAGYLDTNVALQNPDFSAIANGMGFLGLRVARSDELEPALVRALAHDGPALVDVRVDPME
HCCCEECCEEECCCCHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCC
LSMPPKVKAAQVKGFSLYAARAVMNGRGDEIVELAKTNLLRRR
CCCCCCCCHHEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GIFGSSKAKSVADLLVETLAHAGVQRIWGVTGDSLNAINDSLRRHGGIEWMHVRHEEAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCC
AFAAGAEAQVTGRLAVCAGSCGPGNLHLINGLYDCQRNHQPVLAIASHIPSSEIGLGYFQ
CEECCCCCEEECEEEEEECCCCCCCEEEECCHHHHCCCCCCHHHHHHHCCCCCCCCCCCC
ETHPTELFRECSHFCEMVQDPKQLPEVLHRAMRTAIGRHGVAVIVLPGDVSTLEAPEGAR
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
PDFAPPALPRLLPDAAALADLATLLNGSEAVTILAGAGTAGAHDEVVALAKALAAPIVHA
CCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
FRGKEHMEWDNPFDVGMTGLIGFSSGYHAMRECDTLLMLGTDFPYRDFYPEDATIVQIDR
HCCCCCCCCCCCCCCCCHHEEECCCCHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEEC
DPGALGRRAQLRMGLVADVREAAAMLAPLIQPGRDTGFLDKAREHYQAARRDLDGLASPR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AGDEPLHPQFVAATLDRLAADDAVFTFDVGTPAIWAARYLRMNGRRRLLGSFNHGSMANA
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCHHEEECCCCCCHHHH
MPQALGAQAVRPDRQVVSISGDGGLAMLMGDMLTAVQMKLPIKIVLINNGVLGFVQMEQK
HHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEHHH
AAGYLDTNVALQNPDFSAIANGMGFLGLRVARSDELEPALVRALAHDGPALVDVRVDPME
HCCCEECCEEECCCCHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCC
LSMPPKVKAAQVKGFSLYAARAVMNGRGDEIVELAKTNLLRRR
CCCCCCCCHHEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]