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The map label for this gene is metG

Identifier: 120612475

GI number: 120612475

Start: 4262190

End: 4264310

Strand: Direct

Name: metG

Synonym: Aave_3834

Alternate gene names: 120612475

Gene position: 4262190-4264310 (Clockwise)

Preceding gene: 120612474

Following gene: 120612476

Centisome position: 79.63

GC content: 68.08

Gene sequence:

>2121_bases
ATGCCCGCACGCAAGATCTTCGTCACCACCGCCCTGCCGTACGCCAACGGCAACTTCCACATCGGCCACATCATGGAATA
CATCCAGGCCGACATCTGGGTGCGGTTCCAGCGCATGCAGGGCGCCGAGGTGAACTTCGTCGGCGCGGACGACACGCACG
GCGCGCCGATCATGATCGCCGCCGAGAAGGCCGGCAAGACGCCGCAACAGTTCGTGGCCGACATCGCCGCCGGCCGCAAG
CCCTACCTGGAGGGCTTCCACATCCGCTTCGACAACTGGCACAGCACGGATGCGCCCGAGAACCACGAGCTCGCCCGCCA
GATCTACCGCGACCTGCAGGCCGCGGGCCTGATCGAGACGCGCACCATCGAGCAGTTCTTCGACCCCGAGAAGAACATGT
TCCTGCCGGACCGCTTCATCAAGGGCGAATGCCCGCGCTGCCATGCCAGGGACCAGTACGGCGACAACTGCGAGAACTGC
GGCGCGGTGTACGCGCCCACCGACCTGATCGAGCCCTACTCCGCCCTCTCCGGCGCCAAGCCGGTGCTGAAAAGCTCCGA
CCACTTCTTCTTCCAGCTCTCCGACCCGCGCTGCGTGGCCTTCCTGCAGGAATGGACACAGGACGGCCGCCTGCAGCCCG
AGGTGGCCAACAAGGTCAAGGAATGGTTCTCGGTGCGCACCAACCCCGACGGCACCACCAGCGAAGGCCTGGGCGACTGG
GACATCAGCCGCGACGCGCCCTACTTCGGCATCGAGATCCCCGACGCGCCGGGCAAGTACTTCTACGTCTGGCTGGACGC
GCCCGTGGGCTACCTCGCCTCGCTCAAGAACCTGCTGGAAAAGCGCGGCCAGAGCTACGACGACTACGTGGCCGACCCGC
AGCTGGAGCAGGTCCACTTCATCGGCAAGGACATCGTCACCTTCCACACGCTGTTCTGGCCCGCGATGCTGAAGTTCAGC
GGCCGCAAGACGCCGGACGCGGTGTTCGTGCACGGTTTCCTCACCGTGAACAACGGCGAGAAGATGAGCAAGAGCCGCGG
CACGGGGCTCGACCCGCTCAAGTACCTGGGCCTGGGGATGAACGCCGAGTGGCTGCGCTACTACCTCGCGGCCAAGCTCA
ACGGTCGCAACGAGGACATCGACTTCAACGCCGAGGACTTCATGGCGCGGGTCAACAGCGACCTGATCGGCAAGTTCGTG
AACATCGCGAGCCGCGCCGCGGGCTTCCTCACCAAGCGCTTCGGCGGCCGGCTCGGCGCGCCCGATGCCGACGGCGCGGC
GCTGCTGGAGGCCCTGCGCGCCCAGGCCGGCGCCATCGCCGAGGCCTACGAGCGGCGCGACACCGCCCGCGCCGTGCGCG
AAACCATGCTTCTGGCCGACCGCGTGAACGAGTACGTGGATGCGCGCAAGCCCTGGGAACTCGCCAAGCAGGAAGGCCAG
GAGGCCGCGCTGCAGGCTGCCTGCACCACCTGCATCGAGGCCTTCCGCCTGCTCACGCTCTACCTCAAGCCCGTGCTGCC
AGCCCTGGCGGCGCAGGTGGAGGCGTTCCTGAACGTCCAGCCACTCACCTTCGCCGATGCGCCCGCCCTGCTGGGCGAGG
GCCACGCCATCGGTGCCTACCAGCACCTGATGCAGCGCGTGGACATCAAGCAGCTCGAAGCGCTGTTCGAGGTACCGGCG
GCCGCTGCGGCTCCTGCCGCGCCTGCCGCGAACGCGGCGGCCGAAGCCAGTGCCGCGGCCGGTGCCGCCACAGAGGCCCC
CGGCGGCGAGCACATCGCCCCCACCATCACCATCGACGACTTCGCGAAGATCGACCTGCGCATCGCGCTGATCGTGAACT
GCGAGCCGGTCGAGGGCTCCACCAAGCTGCTGCGCCTCACGCTCGACGTGGGCGAAGGCCGCCACCGCAACGTCTTCTCC
GGCATCGCGAGCGCCTACCGCCCCGAGGAACTCGTGGGCAAGCTGACGGTGATGGTCGCCAACCTCGCGCCGCGCAAGAT
GAAGTTCGGCGTCAGCGAGGGCATGGTGCTCGCCGCCAGCCATGGCGACGAGAAGGCGCATCCGGGCATCCACGTGCTGA
ATCCGTGGCCGGGGGCGACGCCCGGCATGCGGGTGCGCTGA

Upstream 100 bases:

>100_bases
GGCGGCCGGCGCGGCGCCGGACCGGGGAGGCTCTTAAAATCATCCGTTGCGCCCGGAGCCCTCCGCCCGGGCCGCACTCC
CCTCAGAAAGCCCGCTCCCG

Downstream 100 bases:

>100_bases
TCGGACCGGCCATGCCCGTCCTACCCCCTGGCCCGGCCGCCCTGCTACGGCTGGCCCTGCCGGCAGCGCTGGCCCTGCTC
GCCGGCTGCACCGCGGTCAG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 706; Mature: 705

Protein sequence:

>706_residues
MPARKIFVTTALPYANGNFHIGHIMEYIQADIWVRFQRMQGAEVNFVGADDTHGAPIMIAAEKAGKTPQQFVADIAAGRK
PYLEGFHIRFDNWHSTDAPENHELARQIYRDLQAAGLIETRTIEQFFDPEKNMFLPDRFIKGECPRCHARDQYGDNCENC
GAVYAPTDLIEPYSALSGAKPVLKSSDHFFFQLSDPRCVAFLQEWTQDGRLQPEVANKVKEWFSVRTNPDGTTSEGLGDW
DISRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASLKNLLEKRGQSYDDYVADPQLEQVHFIGKDIVTFHTLFWPAMLKFS
GRKTPDAVFVHGFLTVNNGEKMSKSRGTGLDPLKYLGLGMNAEWLRYYLAAKLNGRNEDIDFNAEDFMARVNSDLIGKFV
NIASRAAGFLTKRFGGRLGAPDADGAALLEALRAQAGAIAEAYERRDTARAVRETMLLADRVNEYVDARKPWELAKQEGQ
EAALQAACTTCIEAFRLLTLYLKPVLPALAAQVEAFLNVQPLTFADAPALLGEGHAIGAYQHLMQRVDIKQLEALFEVPA
AAAAPAAPAANAAAEASAAAGAATEAPGGEHIAPTITIDDFAKIDLRIALIVNCEPVEGSTKLLRLTLDVGEGRHRNVFS
GIASAYRPEELVGKLTVMVANLAPRKMKFGVSEGMVLAASHGDEKAHPGIHVLNPWPGATPGMRVR

Sequences:

>Translated_706_residues
MPARKIFVTTALPYANGNFHIGHIMEYIQADIWVRFQRMQGAEVNFVGADDTHGAPIMIAAEKAGKTPQQFVADIAAGRK
PYLEGFHIRFDNWHSTDAPENHELARQIYRDLQAAGLIETRTIEQFFDPEKNMFLPDRFIKGECPRCHARDQYGDNCENC
GAVYAPTDLIEPYSALSGAKPVLKSSDHFFFQLSDPRCVAFLQEWTQDGRLQPEVANKVKEWFSVRTNPDGTTSEGLGDW
DISRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASLKNLLEKRGQSYDDYVADPQLEQVHFIGKDIVTFHTLFWPAMLKFS
GRKTPDAVFVHGFLTVNNGEKMSKSRGTGLDPLKYLGLGMNAEWLRYYLAAKLNGRNEDIDFNAEDFMARVNSDLIGKFV
NIASRAAGFLTKRFGGRLGAPDADGAALLEALRAQAGAIAEAYERRDTARAVRETMLLADRVNEYVDARKPWELAKQEGQ
EAALQAACTTCIEAFRLLTLYLKPVLPALAAQVEAFLNVQPLTFADAPALLGEGHAIGAYQHLMQRVDIKQLEALFEVPA
AAAAPAAPAANAAAEASAAAGAATEAPGGEHIAPTITIDDFAKIDLRIALIVNCEPVEGSTKLLRLTLDVGEGRHRNVFS
GIASAYRPEELVGKLTVMVANLAPRKMKFGVSEGMVLAASHGDEKAHPGIHVLNPWPGATPGMRVR
>Mature_705_residues
PARKIFVTTALPYANGNFHIGHIMEYIQADIWVRFQRMQGAEVNFVGADDTHGAPIMIAAEKAGKTPQQFVADIAAGRKP
YLEGFHIRFDNWHSTDAPENHELARQIYRDLQAAGLIETRTIEQFFDPEKNMFLPDRFIKGECPRCHARDQYGDNCENCG
AVYAPTDLIEPYSALSGAKPVLKSSDHFFFQLSDPRCVAFLQEWTQDGRLQPEVANKVKEWFSVRTNPDGTTSEGLGDWD
ISRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASLKNLLEKRGQSYDDYVADPQLEQVHFIGKDIVTFHTLFWPAMLKFSG
RKTPDAVFVHGFLTVNNGEKMSKSRGTGLDPLKYLGLGMNAEWLRYYLAAKLNGRNEDIDFNAEDFMARVNSDLIGKFVN
IASRAAGFLTKRFGGRLGAPDADGAALLEALRAQAGAIAEAYERRDTARAVRETMLLADRVNEYVDARKPWELAKQEGQE
AALQAACTTCIEAFRLLTLYLKPVLPALAAQVEAFLNVQPLTFADAPALLGEGHAIGAYQHLMQRVDIKQLEALFEVPAA
AAAPAAPAANAAAEASAAAGAATEAPGGEHIAPTITIDDFAKIDLRIALIVNCEPVEGSTKLLRLTLDVGEGRHRNVFSG
IASAYRPEELVGKLTVMVANLAPRKMKFGVSEGMVLAASHGDEKAHPGIHVLNPWPGATPGMRVR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=598, Percent_Identity=27.0903010033445, Blast_Score=215, Evalue=1e-55,
Organism=Homo sapiens, GI24308436, Length=541, Percent_Identity=24.7689463955638, Blast_Score=133, Evalue=8e-31,
Organism=Escherichia coli, GI1788432, Length=710, Percent_Identity=53.5211267605634, Blast_Score=733, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=676, Percent_Identity=27.5147928994083, Blast_Score=223, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI212645096, Length=526, Percent_Identity=24.9049429657795, Blast_Score=150, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71992781, Length=331, Percent_Identity=27.190332326284, Blast_Score=110, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=566, Percent_Identity=27.5618374558304, Blast_Score=218, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6321610, Length=557, Percent_Identity=24.7755834829443, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24655311, Length=552, Percent_Identity=29.1666666666667, Blast_Score=211, Evalue=2e-54,
Organism=Drosophila melanogaster, GI21358483, Length=532, Percent_Identity=26.5037593984962, Blast_Score=140, Evalue=4e-33,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_ACIAC (A1TTU1)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_972153.1
- ProteinModelPortal:   A1TTU1
- STRING:   A1TTU1
- GeneID:   4667700
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_3834
- NMPDR:   fig|397945.5.peg.3270
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   RASDHAY
- PhylomeDB:   A1TTU1
- ProtClustDB:   PRK00133
- BioCyc:   AAVE397945:AAVE_3834-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 77579; Mature: 77448

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 344-344

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARKIFVTTALPYANGNFHIGHIMEYIQADIWVRFQRMQGAEVNFVGADDTHGAPIMIA
CCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEE
AEKAGKTPQQFVADIAAGRKPYLEGFHIRFDNWHSTDAPENHELARQIYRDLQAAGLIET
ECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHH
RTIEQFFDPEKNMFLPDRFIKGECPRCHARDQYGDNCENCGAVYAPTDLIEPYSALSGAK
HHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHCCCEECCHHHHCHHHHHCCCC
PVLKSSDHFFFQLSDPRCVAFLQEWTQDGRLQPEVANKVKEWFSVRTNPDGTTSEGLGDW
CHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DISRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASLKNLLEKRGQSYDDYVADPQLEQVHF
CCCCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
IGKDIVTFHTLFWPAMLKFSGRKTPDAVFVHGFLTVNNGEKMSKSRGTGLDPLKYLGLGM
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCHHHHCCCCCCCHHHHHCCCC
NAEWLRYYLAAKLNGRNEDIDFNAEDFMARVNSDLIGKFVNIASRAAGFLTKRFGGRLGA
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PDADGAALLEALRAQAGAIAEAYERRDTARAVRETMLLADRVNEYVDARKPWELAKQEGQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCH
EAALQAACTTCIEAFRLLTLYLKPVLPALAAQVEAFLNVQPLTFADAPALLGEGHAIGAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHH
QHLMQRVDIKQLEALFEVPAAAAAPAAPAANAAAEASAAAGAATEAPGGEHIAPTITIDD
HHHHHHCCHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECC
FAKIDLRIALIVNCEPVEGSTKLLRLTLDVGEGRHRNVFSGIASAYRPEELVGKLTVMVA
CCEEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
NLAPRKMKFGVSEGMVLAASHGDEKAHPGIHVLNPWPGATPGMRVR
HCCCHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
PARKIFVTTALPYANGNFHIGHIMEYIQADIWVRFQRMQGAEVNFVGADDTHGAPIMIA
CCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEE
AEKAGKTPQQFVADIAAGRKPYLEGFHIRFDNWHSTDAPENHELARQIYRDLQAAGLIET
ECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHH
RTIEQFFDPEKNMFLPDRFIKGECPRCHARDQYGDNCENCGAVYAPTDLIEPYSALSGAK
HHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHCCCEECCHHHHCHHHHHCCCC
PVLKSSDHFFFQLSDPRCVAFLQEWTQDGRLQPEVANKVKEWFSVRTNPDGTTSEGLGDW
CHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DISRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASLKNLLEKRGQSYDDYVADPQLEQVHF
CCCCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
IGKDIVTFHTLFWPAMLKFSGRKTPDAVFVHGFLTVNNGEKMSKSRGTGLDPLKYLGLGM
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCHHHHCCCCCCCHHHHHCCCC
NAEWLRYYLAAKLNGRNEDIDFNAEDFMARVNSDLIGKFVNIASRAAGFLTKRFGGRLGA
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PDADGAALLEALRAQAGAIAEAYERRDTARAVRETMLLADRVNEYVDARKPWELAKQEGQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCH
EAALQAACTTCIEAFRLLTLYLKPVLPALAAQVEAFLNVQPLTFADAPALLGEGHAIGAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHH
QHLMQRVDIKQLEALFEVPAAAAAPAAPAANAAAEASAAAGAATEAPGGEHIAPTITIDD
HHHHHHCCHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECC
FAKIDLRIALIVNCEPVEGSTKLLRLTLDVGEGRHRNVFSGIASAYRPEELVGKLTVMVA
CCEEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
NLAPRKMKFGVSEGMVLAASHGDEKAHPGIHVLNPWPGATPGMRVR
HCCCHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA