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The map label for this gene is yiaO [H]

Identifier: 120612100

GI number: 120612100

Start: 3808084

End: 3809133

Strand: Reverse

Name: yiaO [H]

Synonym: Aave_3447

Alternate gene names: 120612100

Gene position: 3809133-3808084 (Counterclockwise)

Preceding gene: 120612101

Following gene: 120612099

Centisome position: 71.16

GC content: 68.57

Gene sequence:

>1050_bases
ATGCGCCACGCTGCCGTTACCGCTCCCGCCGTTTCCTCGCCTTCCCTCCCGCTGCCCGCCCACCGCACCCTGCGCCGCCA
GGGCCTGGCCGCGGCGCTGGCCGCCATCGCCTGCCTGGCCACGGGCACGGTCCACGCCCAGCAGGCCAAGGAAGTCAAGC
TCGGCTACGCGCTGGCCGTCAACTCGCACTACGGTGCGGCCGCCCAGGCGTGGTCCGAAACGGTCGAGAAGGCCACCAAC
GGCGCCATCAAGTTCAAGCAGTTCCCCTCCAGCGCCCTGGGCGGCGAGCGCGAGCTGATCGAGGGCCTGCAACTGGGCAC
CGTGGAGGCCGTCATCGTCTCCACCGGGGCCCTGAGCAACTTCGTGCCCGACGTGGGCGTGGTGGACATCCCCTTCCTGT
TCCGCGACACGGCCCACGCCCGCGCCGTGCTCGACGGCGCCTTCGGCCAGGAACTGCTGGGCAAGTTCCAGAAGCGTGGC
CTCATCGCGCTGGCCTGGGGCGAGCAGGGCTTCCGCCACCTCACCAACAACAAGCATCCGGTCAAGACGCCCGCCGACCT
GAAAGGCCTGAAGATCCGCGTGACCGAGAACCCGGTCCACATCACCGCCTTCCGCACGCTGGGCGCCTCGCCCACGCCCA
TGTCCTGGCCCGAGGTGATCGGCGCGCTGCAGCAGGGCACCATCGACGGGCAGGAGAACCCCATGTCGGTCATCGTCTCG
GCCAAGCTGCCGCAGGTGCAGAAATACCTCTCGCTCACGGGCCACGTCTATGCCCCGATGGCCCTCATCGTTTCGCCCAA
CTTCTGGGCGGGCCTGAACGATGCGCAGAAGGCCGCCTTCACGCAAGGCGCCAAGGCGGGCGCGCTGGCTTCGCGCAAGT
TCGTCGATGACGTGGAGCAGAAGGGCCTGGCCGAAGTCAAGGCCGCCGGCATGCAGGTGGTGGAGTCGGTGGACAAGAGC
GCCTTCCAGAAGGCCCTGGAGCCCGCCTACAAGCAGTACGCCACCAAGTTCGGCCAGAAGACCCTGGACCAGATCGCCAG
CGTGAAGTGA

Upstream 100 bases:

>100_bases
GATGGCGCAACGGTCACCATAATCGCCCGCCGTCCCCGGGCGGCATGCTTTTCCGCTGCCGGCCCTGCCAGGCTTTCCAC
GTTCACCGGACCCCGTACCC

Downstream 100 bases:

>100_bases
GCCGGGGCGGGCGGCATGGGCCGCCTGCCTTGCGGCTCCATCGGCCTGTGCCCGGCGCGTCCGGGCTTTGTCTTTTCCAG
CCCCCATCCATCCCGCCATG

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MRHAAVTAPAVSSPSLPLPAHRTLRRQGLAAALAAIACLATGTVHAQQAKEVKLGYALAVNSHYGAAAQAWSETVEKATN
GAIKFKQFPSSALGGERELIEGLQLGTVEAVIVSTGALSNFVPDVGVVDIPFLFRDTAHARAVLDGAFGQELLGKFQKRG
LIALAWGEQGFRHLTNNKHPVKTPADLKGLKIRVTENPVHITAFRTLGASPTPMSWPEVIGALQQGTIDGQENPMSVIVS
AKLPQVQKYLSLTGHVYAPMALIVSPNFWAGLNDAQKAAFTQGAKAGALASRKFVDDVEQKGLAEVKAAGMQVVESVDKS
AFQKALEPAYKQYATKFGQKTLDQIASVK

Sequences:

>Translated_349_residues
MRHAAVTAPAVSSPSLPLPAHRTLRRQGLAAALAAIACLATGTVHAQQAKEVKLGYALAVNSHYGAAAQAWSETVEKATN
GAIKFKQFPSSALGGERELIEGLQLGTVEAVIVSTGALSNFVPDVGVVDIPFLFRDTAHARAVLDGAFGQELLGKFQKRG
LIALAWGEQGFRHLTNNKHPVKTPADLKGLKIRVTENPVHITAFRTLGASPTPMSWPEVIGALQQGTIDGQENPMSVIVS
AKLPQVQKYLSLTGHVYAPMALIVSPNFWAGLNDAQKAAFTQGAKAGALASRKFVDDVEQKGLAEVKAAGMQVVESVDKS
AFQKALEPAYKQYATKFGQKTLDQIASVK
>Mature_349_residues
MRHAAVTAPAVSSPSLPLPAHRTLRRQGLAAALAAIACLATGTVHAQQAKEVKLGYALAVNSHYGAAAQAWSETVEKATN
GAIKFKQFPSSALGGERELIEGLQLGTVEAVIVSTGALSNFVPDVGVVDIPFLFRDTAHARAVLDGAFGQELLGKFQKRG
LIALAWGEQGFRHLTNNKHPVKTPADLKGLKIRVTENPVHITAFRTLGASPTPMSWPEVIGALQQGTIDGQENPMSVIVS
AKLPQVQKYLSLTGHVYAPMALIVSPNFWAGLNDAQKAAFTQGAKAGALASRKFVDDVEQKGLAEVKAAGMQVVESVDKS
AFQKALEPAYKQYATKFGQKTLDQIASVK

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=303, Percent_Identity=37.953795379538, Blast_Score=225, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 37021; Mature: 37021

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHAAVTAPAVSSPSLPLPAHRTLRRQGLAAALAAIACLATGTVHAQQAKEVKLGYALAV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEEE
NSHYGAAAQAWSETVEKATNGAIKFKQFPSSALGGERELIEGLQLGTVEAVIVSTGALSN
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHCCCHHHHHHHHCCCCCEEEEEEHHHHHH
FVPDVGVVDIPFLFRDTAHARAVLDGAFGQELLGKFQKRGLIALAWGEQGFRHLTNNKHP
HCCCCCCEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCC
VKTPADLKGLKIRVTENPVHITAFRTLGASPTPMSWPEVIGALQQGTIDGQENPMSVIVS
CCCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEE
AKLPQVQKYLSLTGHVYAPMALIVSPNFWAGLNDAQKAAFTQGAKAGALASRKFVDDVEQ
CCCHHHHHHHHHCCHHHHCEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
KGLAEVKAAGMQVVESVDKSAFQKALEPAYKQYATKFGQKTLDQIASVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRHAAVTAPAVSSPSLPLPAHRTLRRQGLAAALAAIACLATGTVHAQQAKEVKLGYALAV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEEE
NSHYGAAAQAWSETVEKATNGAIKFKQFPSSALGGERELIEGLQLGTVEAVIVSTGALSN
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHCCCHHHHHHHHCCCCCEEEEEEHHHHHH
FVPDVGVVDIPFLFRDTAHARAVLDGAFGQELLGKFQKRGLIALAWGEQGFRHLTNNKHP
HCCCCCCEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCC
VKTPADLKGLKIRVTENPVHITAFRTLGASPTPMSWPEVIGALQQGTIDGQENPMSVIVS
CCCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEE
AKLPQVQKYLSLTGHVYAPMALIVSPNFWAGLNDAQKAAFTQGAKAGALASRKFVDDVEQ
CCCHHHHHHHHHCCHHHHCEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
KGLAEVKAAGMQVVESVDKSAFQKALEPAYKQYATKFGQKTLDQIASVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]