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The map label for this gene is purN [H]

Identifier: 120612090

GI number: 120612090

Start: 3795677

End: 3796255

Strand: Reverse

Name: purN [H]

Synonym: Aave_3437

Alternate gene names: 120612090

Gene position: 3796255-3795677 (Counterclockwise)

Preceding gene: 120612097

Following gene: 120612086

Centisome position: 70.92

GC content: 70.81

Gene sequence:

>579_bases
ATGAAGAACATCGTGATTTTGATCTCGGGCGGGGGCTCCAACATGGCGGCCATCGTGCGGACGGCCCGCATGCAGGACTG
GGCAGGCCGCCACGGCGTGCGGGTGGCGGCCGTGCTGAGCAACAAGGCCGATGCACCCGGCCTCGCCTGGGCGCGTGAGC
AGGGCATCGCCACGGACGCCGTGGACCACCGGGCCCACGCTTCGCGCGAGGCCTTCGATGCAGCACTCGCGCAGCGGATC
GACACACACGATCCCGCGCTCGTCGTGCTGGCCGGCTTCATGCGCATCCTCACGCCCGGCTTCGTGGCGCATTACGCAGG
GCGGCTGGTCAACATCCACCCTTCGCTGCTGCCGGCCTTTCCCGGCCTGCATACCCACCAGCGCGCCATCGACGCGGGCT
GCAAGGTGGCCGGGGCCTCCGTGCACCTCGTCACCCCCGAACTGGACGCGGGGCCCATCCTGGCCCAGGGCGTCGTGCCG
GTGCTGCCGGGGGACACGGCCGAGCGCCTGTCCGAGCGGGTGCTGGCGCAGGAGCATGCGATCTACGCACCGGCCGTGCT
GCAGTTGCTGCTGTCCTGA

Upstream 100 bases:

>100_bases
GGTCAGGGGCTGGAGGCGGGGATCGAAGGGCATGGAAACAAGGCGGGTGGCACCGCCGCAGGCAAGGAGGAGGAACGGAC
GCGGCGATAATCCGCGCCCC

Downstream 100 bases:

>100_bases
CGCTTTGGCCGATACGGCCATGCGGCCAGCGCGGTGAGCCGGCGGATCAGAGCGCCGTTACGATCTTTCCGGGGTGGCTG
CCGTCCAGCAGGTCCCTGGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MKNIVILISGGGSNMAAIVRTARMQDWAGRHGVRVAAVLSNKADAPGLAWAREQGIATDAVDHRAHASREAFDAALAQRI
DTHDPALVVLAGFMRILTPGFVAHYAGRLVNIHPSLLPAFPGLHTHQRAIDAGCKVAGASVHLVTPELDAGPILAQGVVP
VLPGDTAERLSERVLAQEHAIYAPAVLQLLLS

Sequences:

>Translated_192_residues
MKNIVILISGGGSNMAAIVRTARMQDWAGRHGVRVAAVLSNKADAPGLAWAREQGIATDAVDHRAHASREAFDAALAQRI
DTHDPALVVLAGFMRILTPGFVAHYAGRLVNIHPSLLPAFPGLHTHQRAIDAGCKVAGASVHLVTPELDAGPILAQGVVP
VLPGDTAERLSERVLAQEHAIYAPAVLQLLLS
>Mature_192_residues
MKNIVILISGGGSNMAAIVRTARMQDWAGRHGVRVAAVLSNKADAPGLAWAREQGIATDAVDHRAHASREAFDAALAQRI
DTHDPALVVLAGFMRILTPGFVAHYAGRLVNIHPSLLPAFPGLHTHQRAIDAGCKVAGASVHLVTPELDAGPILAQGVVP
VLPGDTAERLSERVLAQEHAIYAPAVLQLLLS

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=189, Percent_Identity=42.3280423280423, Blast_Score=150, Evalue=8e-37,
Organism=Homo sapiens, GI209869995, Length=189, Percent_Identity=42.3280423280423, Blast_Score=150, Evalue=8e-37,
Organism=Homo sapiens, GI209869993, Length=189, Percent_Identity=42.3280423280423, Blast_Score=150, Evalue=8e-37,
Organism=Escherichia coli, GI1788846, Length=180, Percent_Identity=55.5555555555556, Blast_Score=194, Evalue=2e-51,
Organism=Escherichia coli, GI1787483, Length=169, Percent_Identity=35.5029585798817, Blast_Score=96, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=184, Percent_Identity=39.1304347826087, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320616, Length=214, Percent_Identity=30.3738317757009, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582400, Length=191, Percent_Identity=41.8848167539267, Blast_Score=147, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20207; Mature: 20207

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIVILISGGGSNMAAIVRTARMQDWAGRHGVRVAAVLSNKADAPGLAWAREQGIATDA
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHCCCCHHH
VDHRAHASREAFDAALAQRIDTHDPALVVLAGFMRILTPGFVAHYAGRLVNIHPSLLPAF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHCCEEEECHHHHHCC
PGLHTHQRAIDAGCKVAGASVHLVTPELDAGPILAQGVVPVLPGDTAERLSERVLAQEHA
CCCCHHHHHHHHCCEECCCEEEEECCCCCCCCCHHCCCEECCCCCHHHHHHHHHHHHHHH
IYAPAVLQLLLS
HHHHHHHHHHHC
>Mature Secondary Structure
MKNIVILISGGGSNMAAIVRTARMQDWAGRHGVRVAAVLSNKADAPGLAWAREQGIATDA
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHCCCCHHH
VDHRAHASREAFDAALAQRIDTHDPALVVLAGFMRILTPGFVAHYAGRLVNIHPSLLPAF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHCCEEEECHHHHHCC
PGLHTHQRAIDAGCKVAGASVHLVTPELDAGPILAQGVVPVLPGDTAERLSERVLAQEHA
CCCCHHHHHHHHCCEECCCEEEEECCCCCCCCCHHCCCEECCCCCHHHHHHHHHHHHHHH
IYAPAVLQLLLS
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]