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The map label for this gene is fabI [H]

Identifier: 120611913

GI number: 120611913

Start: 3598750

End: 3599547

Strand: Direct

Name: fabI [H]

Synonym: Aave_3255

Alternate gene names: 120611913

Gene position: 3598750-3599547 (Clockwise)

Preceding gene: 120611908

Following gene: 120611916

Centisome position: 67.23

GC content: 66.79

Gene sequence:

>798_bases
ATGGGTTTCCTGACCGGCAAGAAGCTGCTCATCACCGGCGTGCTGTCCAACCGTTCCATCGCCTACGGCATCGCCAAGGC
CTGCCACGCGCAGGGTGCCGAGCTGGCCTTCAGCTACGTGGGCGAGCGCTTCAAGGACCGCATCACCGAATTCGCGGCCG
AATTCGATTCCAGGCTGGTCTTCGATTGCGACGTGGCGAGCGACGAGCAGATCGAGCGCATGTTCGCCGACCTGTCGGCC
ACCTGGCCGAAGTTCGACGGCTTCGTGCACAGCATCGGTTTCGCTCCGCGTGAAGCCATCGCTGGCGACTTCCTCGAAGG
CCTGTCGCGCGAATCCTTCCGCATCGCGCACGACATCAGCGCCTACAGCTTCCCCGCCATGGCCAAGGCCGCCCTGCCCT
ACCTGAACGACAAGGCCGCCGTGCTCACCCTGAGCTACCTGGGCGCACTGCGCGCGGTGCCCAACTACAACACCATGGGC
CTGGCCAAGGCCAGCCTGGAAGCCAGCGTGCGCTACCTGGCCGAATCCCTGGGCCCCAGGGGCATGCGCGCCAACGGCAT
CAGCGCAGGCCCGATCAAGACACTGGCCGCCAGCGGCATCAAGGACTTCGGCAAGCTGCTGGGCACCGTGGCCGACGCCT
CGCCCCTGCGCCGCAACGTGACCATCGAGGACGTGGGCAATGCCGCGGCCTTCCTGCTGTCGGACCTCGCCAGCGGCGTG
ACGGCGGAAATCATGTACGTGGACGGCGGCTTCAGCCAGACCGGCGGCATCAGCCGCGACAGCGCCAACCTGGCCTGA

Upstream 100 bases:

>100_bases
GATTTTCCCGGTCGGATTCCCGGCCGCCGCATCTTTCCGCCACGGGCCGCGCCGCGCCCCTCCGGCATTCCACCCCTACC
GCATTCACTGGAGAACAACA

Downstream 100 bases:

>100_bases
TCGCCAGGCCGCGTCGGCGGGCGACCGAACCGATGCACGGACAGCCAAAGCCGCAAGGTCCCGCCATGGGGCGGCCTCGC
GGCTTTCGCACATCCGGCCA

Product: enoyl-[acyl-carrier-protein] reductase [NADH]

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MGFLTGKKLLITGVLSNRSIAYGIAKACHAQGAELAFSYVGERFKDRITEFAAEFDSRLVFDCDVASDEQIERMFADLSA
TWPKFDGFVHSIGFAPREAIAGDFLEGLSRESFRIAHDISAYSFPAMAKAALPYLNDKAAVLTLSYLGALRAVPNYNTMG
LAKASLEASVRYLAESLGPRGMRANGISAGPIKTLAASGIKDFGKLLGTVADASPLRRNVTIEDVGNAAAFLLSDLASGV
TAEIMYVDGGFSQTGGISRDSANLA

Sequences:

>Translated_265_residues
MGFLTGKKLLITGVLSNRSIAYGIAKACHAQGAELAFSYVGERFKDRITEFAAEFDSRLVFDCDVASDEQIERMFADLSA
TWPKFDGFVHSIGFAPREAIAGDFLEGLSRESFRIAHDISAYSFPAMAKAALPYLNDKAAVLTLSYLGALRAVPNYNTMG
LAKASLEASVRYLAESLGPRGMRANGISAGPIKTLAASGIKDFGKLLGTVADASPLRRNVTIEDVGNAAAFLLSDLASGV
TAEIMYVDGGFSQTGGISRDSANLA
>Mature_264_residues
GFLTGKKLLITGVLSNRSIAYGIAKACHAQGAELAFSYVGERFKDRITEFAAEFDSRLVFDCDVASDEQIERMFADLSAT
WPKFDGFVHSIGFAPREAIAGDFLEGLSRESFRIAHDISAYSFPAMAKAALPYLNDKAAVLTLSYLGALRAVPNYNTMGL
AKASLEASVRYLAESLGPRGMRANGISAGPIKTLAASGIKDFGKLLGTVADASPLRRNVTIEDVGNAAAFLLSDLASGVT
AEIMYVDGGFSQTGGISRDSANLA

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=252, Percent_Identity=63.0952380952381, Blast_Score=339, Evalue=9e-95,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=28.1853281853282, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787905, Length=258, Percent_Identity=26.7441860465116, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=257, Percent_Identity=25.6809338521401, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=219, Percent_Identity=30.5936073059361, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24643142, Length=257, Percent_Identity=25.6809338521401, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28113; Mature: 27982

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLTGKKLLITGVLSNRSIAYGIAKACHAQGAELAFSYVGERFKDRITEFAAEFDSRLV
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
FDCDVASDEQIERMFADLSATWPKFDGFVHSIGFAPREAIAGDFLEGLSRESFRIAHDIS
EEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCC
AYSFPAMAKAALPYLNDKAAVLTLSYLGALRAVPNYNTMGLAKASLEASVRYLAESLGPR
HHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GMRANGISAGPIKTLAASGIKDFGKLLGTVADASPLRRNVTIEDVGNAAAFLLSDLASGV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCC
TAEIMYVDGGFSQTGGISRDSANLA
EEEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GFLTGKKLLITGVLSNRSIAYGIAKACHAQGAELAFSYVGERFKDRITEFAAEFDSRLV
CCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
FDCDVASDEQIERMFADLSATWPKFDGFVHSIGFAPREAIAGDFLEGLSRESFRIAHDIS
EEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCC
AYSFPAMAKAALPYLNDKAAVLTLSYLGALRAVPNYNTMGLAKASLEASVRYLAESLGPR
HHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GMRANGISAGPIKTLAASGIKDFGKLLGTVADASPLRRNVTIEDVGNAAAFLLSDLASGV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCC
TAEIMYVDGGFSQTGGISRDSANLA
EEEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]