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The map label for this gene is purB [H]

Identifier: 120611868

GI number: 120611868

Start: 3539784

End: 3541142

Strand: Direct

Name: purB [H]

Synonym: Aave_3210

Alternate gene names: 120611868

Gene position: 3539784-3541142 (Clockwise)

Preceding gene: 120611867

Following gene: 120611869

Centisome position: 66.13

GC content: 68.14

Gene sequence:

>1359_bases
ATGCCCGTTTCCGTATTCGACATGCAGTCGCTCCAGCACCTCTGGGGCACCGACGAGTTGCGCACCATCTTCGGCGAAGA
GAACCGCGTGCAGAAGTGGCTCGATTTCGAGGCCGCGCTGGCCGCGGCCCAGGCCGAGATGGGCATCATCCCCGCGGCGG
CCGCCAGGGAGATCGCAGAGAAGGCCCGGGTGGAGAACATCGACATCGCGAAGATGTCGGCCGAAATCCGCCGCATCAAG
CACTCGCTGGTGCCCGCACTCCGGCAGCTCCAGGCCGCCTGCTCCAGGGAGAACGGCGAATGGGTGCACTACGGCGCGAC
CACGCAGGACGTGGTGGACACCGGCGTGGCGCTGCAGCTCAAGGAATTCCACGCCGTGGCGATGCGCGACCTGCAGGCCG
TGGGCCGGGAGCTGGCCCGCCTGGCCGTGGCGCACCGCGACGTGCCCATGGCCGGCCGCACGCACGGCGTGCAGGCGCTG
CCCATCACCTTCGGCCACAAGTGCGCGCTGTGGCTGGACGAGCTCGGCCGCCACCACGAACGCCTGGTGCAGGCCGCGCC
GCGCGTGCTGGTGGGCATGCTGGCGGGCGCCGTCGGCAGCCAGGCGTCGCTGGGCCCGAAGGCCATCGAGCTGGAGGAGC
GCATGCTGGGCAAGCTGGGCCTGGGCGTGCCCGCGATCAGCTGGGCCCCCGCGCGCGACCGCTTCGCCGAATACACGAAC
CTGCTCGCCATGCTCGGCAGCACGCTTTCCAAGATCGGTAACGAGCTGTTCAACATGCAGCGCAACGAGTTCGGCGAAGT
GGAGGAAGGCTTCTCGGAGGGCAAGCTGGGCTCCTCCACCATGCCGCACAAACGCAATCCCACGTCCGCCGAGAACCTGG
CGGGCCTCTCGCGCCCGCTGCGCTACAACGCCGCGATGATGGTCGAGGGCATGGTTTCCGAGAGCGAGCGCGACGGCATC
GCCTGGAAGATGGAATGGAAGGCCGTGCCCGAGTGCTGCATGATCGCCGGCGCCATGCTGTTCCAGGCGAAGAACCTGCT
GGCGGGCCTAAAGGTCAATGCCGACGTGATGGCCGCCAACCTCGACAAGATGCGCGGCTACCTGCTCTCCGAACGGGTCA
TGCTGGAACTGAGCGAACGCGTGGGCAAGCAGACCGCGCACGAATGGATCTACGAGGCTTCCATGCACGGCATCACCCAC
AAGCTCGGCTTCGCCGACGCCATGCGCCAGCACAAGGGCCTGGGCGAGCTGCTGACCGACGCGGAAATCCAGGACCTGAC
CGATCCCGCGGGCTACCTCGGCCAGTGCGGCACCTCGGTGGACCGCGTCGTCGCCGCGCAGAAGTCCTGGCTGGACTGA

Upstream 100 bases:

>100_bases
CGCAACACATTCAACCCGGGCCGCATGCTGCCGCGCGCGCTCGTGGAAGCCACCGCGTCCCTTTCCGACCACTGAACCCC
GACACTCCGACAGGACACCC

Downstream 100 bases:

>100_bases
CCTGCGCCCCCCGGCGGAGGCGGGACCACCCGCCCCGCATTCCTGCAAGGAGACAAGACCCATGTTCGATACCCTTCCCC
GCCGCCGGCTCGGCGCGCTG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MPVSVFDMQSLQHLWGTDELRTIFGEENRVQKWLDFEAALAAAQAEMGIIPAAAAREIAEKARVENIDIAKMSAEIRRIK
HSLVPALRQLQAACSRENGEWVHYGATTQDVVDTGVALQLKEFHAVAMRDLQAVGRELARLAVAHRDVPMAGRTHGVQAL
PITFGHKCALWLDELGRHHERLVQAAPRVLVGMLAGAVGSQASLGPKAIELEERMLGKLGLGVPAISWAPARDRFAEYTN
LLAMLGSTLSKIGNELFNMQRNEFGEVEEGFSEGKLGSSTMPHKRNPTSAENLAGLSRPLRYNAAMMVEGMVSESERDGI
AWKMEWKAVPECCMIAGAMLFQAKNLLAGLKVNADVMAANLDKMRGYLLSERVMLELSERVGKQTAHEWIYEASMHGITH
KLGFADAMRQHKGLGELLTDAEIQDLTDPAGYLGQCGTSVDRVVAAQKSWLD

Sequences:

>Translated_452_residues
MPVSVFDMQSLQHLWGTDELRTIFGEENRVQKWLDFEAALAAAQAEMGIIPAAAAREIAEKARVENIDIAKMSAEIRRIK
HSLVPALRQLQAACSRENGEWVHYGATTQDVVDTGVALQLKEFHAVAMRDLQAVGRELARLAVAHRDVPMAGRTHGVQAL
PITFGHKCALWLDELGRHHERLVQAAPRVLVGMLAGAVGSQASLGPKAIELEERMLGKLGLGVPAISWAPARDRFAEYTN
LLAMLGSTLSKIGNELFNMQRNEFGEVEEGFSEGKLGSSTMPHKRNPTSAENLAGLSRPLRYNAAMMVEGMVSESERDGI
AWKMEWKAVPECCMIAGAMLFQAKNLLAGLKVNADVMAANLDKMRGYLLSERVMLELSERVGKQTAHEWIYEASMHGITH
KLGFADAMRQHKGLGELLTDAEIQDLTDPAGYLGQCGTSVDRVVAAQKSWLD
>Mature_451_residues
PVSVFDMQSLQHLWGTDELRTIFGEENRVQKWLDFEAALAAAQAEMGIIPAAAAREIAEKARVENIDIAKMSAEIRRIKH
SLVPALRQLQAACSRENGEWVHYGATTQDVVDTGVALQLKEFHAVAMRDLQAVGRELARLAVAHRDVPMAGRTHGVQALP
ITFGHKCALWLDELGRHHERLVQAAPRVLVGMLAGAVGSQASLGPKAIELEERMLGKLGLGVPAISWAPARDRFAEYTNL
LAMLGSTLSKIGNELFNMQRNEFGEVEEGFSEGKLGSSTMPHKRNPTSAENLAGLSRPLRYNAAMMVEGMVSESERDGIA
WKMEWKAVPECCMIAGAMLFQAKNLLAGLKVNADVMAANLDKMRGYLLSERVMLELSERVGKQTAHEWIYEASMHGITHK
LGFADAMRQHKGLGELLTDAEIQDLTDPAGYLGQCGTSVDRVVAAQKSWLD

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=459, Percent_Identity=23.0936819172113, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI183227688, Length=395, Percent_Identity=23.2911392405063, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1787376, Length=304, Percent_Identity=26.9736842105263, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790398, Length=204, Percent_Identity=29.9019607843137, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=451, Percent_Identity=23.9467849223947, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32564234, Length=294, Percent_Identity=25.5102040816327, Blast_Score=85, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323391, Length=459, Percent_Identity=23.3115468409586, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24647570, Length=296, Percent_Identity=26.0135135135135, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221473854, Length=293, Percent_Identity=26.2798634812287, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI78706858, Length=293, Percent_Identity=26.2798634812287, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49600; Mature: 49469

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSVFDMQSLQHLWGTDELRTIFGEENRVQKWLDFEAALAAAQAEMGIIPAAAAREIAE
CCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KARVENIDIAKMSAEIRRIKHSLVPALRQLQAACSRENGEWVHYGATTQDVVDTGVALQL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHHH
KEFHAVAMRDLQAVGRELARLAVAHRDVPMAGRTHGVQALPITFGHKCALWLDELGRHHE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
RLVQAAPRVLVGMLAGAVGSQASLGPKAIELEERMLGKLGLGVPAISWAPARDRFAEYTN
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LLAMLGSTLSKIGNELFNMQRNEFGEVEEGFSEGKLGSSTMPHKRNPTSAENLAGLSRPL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCH
RYNAAMMVEGMVSESERDGIAWKMEWKAVPECCMIAGAMLFQAKNLLAGLKVNADVMAAN
HCCHHHHHHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LDKMRGYLLSERVMLELSERVGKQTAHEWIYEASMHGITHKLGFADAMRQHKGLGELLTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
AEIQDLTDPAGYLGQCGTSVDRVVAAQKSWLD
CHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVSVFDMQSLQHLWGTDELRTIFGEENRVQKWLDFEAALAAAQAEMGIIPAAAAREIAE
CCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KARVENIDIAKMSAEIRRIKHSLVPALRQLQAACSRENGEWVHYGATTQDVVDTGVALQL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHHH
KEFHAVAMRDLQAVGRELARLAVAHRDVPMAGRTHGVQALPITFGHKCALWLDELGRHHE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
RLVQAAPRVLVGMLAGAVGSQASLGPKAIELEERMLGKLGLGVPAISWAPARDRFAEYTN
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LLAMLGSTLSKIGNELFNMQRNEFGEVEEGFSEGKLGSSTMPHKRNPTSAENLAGLSRPL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCH
RYNAAMMVEGMVSESERDGIAWKMEWKAVPECCMIAGAMLFQAKNLLAGLKVNADVMAAN
HCCHHHHHHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LDKMRGYLLSERVMLELSERVGKQTAHEWIYEASMHGITHKLGFADAMRQHKGLGELLTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
AEIQDLTDPAGYLGQCGTSVDRVVAAQKSWLD
CHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]