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The map label for this gene is lexA

Identifier: 120611227

GI number: 120611227

Start: 2810753

End: 2811427

Strand: Reverse

Name: lexA

Synonym: Aave_2558

Alternate gene names: 120611227

Gene position: 2811427-2810753 (Counterclockwise)

Preceding gene: 120611229

Following gene: 120611226

Centisome position: 52.52

GC content: 66.52

Gene sequence:

>675_bases
ATGCTCGACAGCCCCAAGCTCACTGCCCGCCAACAGCAGATTCTGGATCTCATCCAGACCGCCATTGCACGCACCGGCGC
GCCCCCCACCCGGGCGGAAATCGCCGCGGAGTTCGGCTTCAAGTCCGCCAATGCGGCCGAAGAGCACCTGCAGGCACTGG
CACGCAAGGGCGTCATCGAGCTGGTGAGCGGCACGTCGCGTGGCATCCGGCTGCGCGGCGAGGCCGTGCGGTCCATCAAT
GCGGCCCGCGGCACCCAGTTCCACCTGCCCATTCCCGGCATCTCGCAGCTCATGCTGCCGCTCATCGGCCGTGTCGCCGC
AGGCTCACCGATTCTCGCAGAGGAACACGTGGACCAGACCTACAGCGTGGAAGGCAGCCTCTTCCAGCACAAGCCCGACT
ATCTGCTCAAGGTCCGGGGCATGTCCATGCGCGATGCCGGCATCATGGATGGCGACCTGCTCGCCGTGCAGTCCACCCGG
GAGGCGCGCAACGGCCAGATCATCGTCGCCCGCCTGGGTGACGAAGTTACCGTCAAGCGGCTGCGGCGCACCGCGGGCGC
CATCGAACTGCTGCCCGAGAACCCCGACTATCCCATCATCACCGTGCAGCCCGGCGAATCGTTCGAGATCGAAGGGCTGG
CCGTGGGCCTCATCCGCAACACCATGCTGATGTAG

Upstream 100 bases:

>100_bases
TTGCAAACTGTTTAAAAATACAGTGACTGGATCGATCCACAGGTGAACCGATCCGCCCAGCGCCCGTCCGGCGCGATCTT
CCTTCACTGGAGTGCCCCGC

Downstream 100 bases:

>100_bases
CCGGCCGCGGGCCGCCCGGCGCATGGCGCCGGAACGCGGTTCGCGCCCTGTTTTCCCGTTTGGCGTTGCCCAGGTGGCGG
GCGTCTGGCTTTCCTCTTCG

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MLDSPKLTARQQQILDLIQTAIARTGAPPTRAEIAAEFGFKSANAAEEHLQALARKGVIELVSGTSRGIRLRGEAVRSIN
AARGTQFHLPIPGISQLMLPLIGRVAAGSPILAEEHVDQTYSVEGSLFQHKPDYLLKVRGMSMRDAGIMDGDLLAVQSTR
EARNGQIIVARLGDEVTVKRLRRTAGAIELLPENPDYPIITVQPGESFEIEGLAVGLIRNTMLM

Sequences:

>Translated_224_residues
MLDSPKLTARQQQILDLIQTAIARTGAPPTRAEIAAEFGFKSANAAEEHLQALARKGVIELVSGTSRGIRLRGEAVRSIN
AARGTQFHLPIPGISQLMLPLIGRVAAGSPILAEEHVDQTYSVEGSLFQHKPDYLLKVRGMSMRDAGIMDGDLLAVQSTR
EARNGQIIVARLGDEVTVKRLRRTAGAIELLPENPDYPIITVQPGESFEIEGLAVGLIRNTMLM
>Mature_224_residues
MLDSPKLTARQQQILDLIQTAIARTGAPPTRAEIAAEFGFKSANAAEEHLQALARKGVIELVSGTSRGIRLRGEAVRSIN
AARGTQFHLPIPGISQLMLPLIGRVAAGSPILAEEHVDQTYSVEGSLFQHKPDYLLKVRGMSMRDAGIMDGDLLAVQSTR
EARNGQIIVARLGDEVTVKRLRRTAGAIELLPENPDYPIITVQPGESFEIEGLAVGLIRNTMLM

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=215, Percent_Identity=56.7441860465116, Blast_Score=236, Evalue=8e-64,
Organism=Escherichia coli, GI1787431, Length=123, Percent_Identity=34.1463414634146, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_ACIAC (A1TQ93)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_970905.1
- ProteinModelPortal:   A1TQ93
- STRING:   A1TQ93
- MEROPS:   S24.001
- GeneID:   4668285
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_2558
- NMPDR:   fig|397945.5.peg.2193
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   VGVWRGY
- PhylomeDB:   A1TQ93
- ProtClustDB:   PRK00215
- BioCyc:   AAVE397945:AAVE_2558-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 24266; Mature: 24266

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: ACT_SITE 142-142 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSPKLTARQQQILDLIQTAIARTGAPPTRAEIAAEFGFKSANAAEEHLQALARKGVIE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHE
LVSGTSRGIRLRGEAVRSINAARGTQFHLPIPGISQLMLPLIGRVAAGSPILAEEHVDQT
ECCCCCCCEEECHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHCCCE
YSVEGSLFQHKPDYLLKVRGMSMRDAGIMDGDLLAVQSTREARNGQIIVARLGDEVTVKR
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCHHHHHH
LRRTAGAIELLPENPDYPIITVQPGESFEIEGLAVGLIRNTMLM
HHHHCCCEEECCCCCCCCEEEECCCCCEEECCEEEHHHHHHHCC
>Mature Secondary Structure
MLDSPKLTARQQQILDLIQTAIARTGAPPTRAEIAAEFGFKSANAAEEHLQALARKGVIE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHE
LVSGTSRGIRLRGEAVRSINAARGTQFHLPIPGISQLMLPLIGRVAAGSPILAEEHVDQT
ECCCCCCCEEECHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHCCCE
YSVEGSLFQHKPDYLLKVRGMSMRDAGIMDGDLLAVQSTREARNGQIIVARLGDEVTVKR
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCHHHHHH
LRRTAGAIELLPENPDYPIITVQPGESFEIEGLAVGLIRNTMLM
HHHHCCCEEECCCCCCCCEEEECCCCCEEECCEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA