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The map label for this gene is dapE

Identifier: 120611153

GI number: 120611153

Start: 2715206

End: 2716375

Strand: Reverse

Name: dapE

Synonym: Aave_2482

Alternate gene names: 120611153

Gene position: 2716375-2715206 (Counterclockwise)

Preceding gene: 120611154

Following gene: 120611152

Centisome position: 50.75

GC content: 70.0

Gene sequence:

>1170_bases
ATGTCCCGTACCCTGATCCTGGCCGAACAGCTCATCTCCCGTCCGTCCGTCACGCCCGAAGATGCCGGCTGCCTCGAACT
GCTGGCCGGGCGCCTGGCCCCGCTGGGCTTCACTTGCGAGCGCATGGACAGCGGGCCGGACAGCTTCCGCGTCAGCAACC
TCTGGGCACGCCGCGCCGCGGCGGGCGGCGGCGCCCCCGCGCGGACCCTGGTGTTCGCGGGCCATACGGACGTAGTGCCC
ACCGGGCCGCCGGAGCAGTGGAGCAGCCCGCCGTTCACGCCCTCCCACCGGGACGGGCGGCTCTACGGCCGCGGCGCCAG
CGACATGAAGACCTCCATCGCCGCCTTCGTCGTGGCGGTGGAGGAGTTCCTGGCCGCCACGCCCGAACCCGCCATCGCGC
TGGCCTTCCTGCTGACCAGCGACGAGGAAGGTCCCTCGGTGGACGGCACCAAGGTGGTGGTGGAGCAGCTCGCCGCCCGC
GGCGAGACGCTGGACTGGTGCATCGTGGGCGAGCCCACCTCCGTGCGCAAGACCGGCGACATGATCAAGAACGGCCGCCG
CGGCACCCTGTCGGGCAAGCTCACCGTGCGCGGCATCCAGGGCCACATCGCCTACCCCCAGCTCGCCCGCAACCCCATCC
ACCAGGCGCTGCCGGCCCTCGCGGAACTGGCCGCCACGGAGTGGGACCGCGGCAACGACTTCTTTCCGCCCACCAGCTGG
CAGATCAGCAACATCCACGGCGGCACGGGTGCGACCAATGTCATTCCCGGCACCGTGGTGGTGGACTTCAATTTCCGCTT
CTGCACCGAATCCACGGCAGAAGGGCTGAAAACCCGCGTGCACAACCTGCTGGACCGGCACGGCCTCGAATACGACCTCA
CCTGGACGCTCGGGGGCCAGCCCTTCCTCACGACGCCCGGCGAGCTGGTGGCCGCGGTGCAGCAGGCGATCACGGACGAA
ACCGGCATCACCACCGAACTGTCCACCACGGGCGGCACCAGCGACGGGCGCTTCATCGCCCGCGTCTGCCCGCAGGTCAT
CGAACTGGGCCCGCCCAACGCCACCATCCACAAGATCGACGAGCACGTGGTGATCGCCGACGTGGAACCGCTCAAGAACA
TCTACCGCCGCACGCTGGAGCGGCTGAACGCACAGGCCGCCGCAGCATGA

Upstream 100 bases:

>100_bases
CAGTGATCCCGCGCGCCGGCCCGGGCCCGTGCACCGGGCCTCGGCCGGCGCCATTTTTCCACCTTCCCCCAGGAGCGTTC
CAGCGCGCTCCGCGTCCACG

Downstream 100 bases:

>100_bases
GCACGACGCCCCACCCCGTCCCGCCCGCGGGCCCCGTTCGCGGCGATACCGTGCCGGAACTGGTCGCGAGCGCGGCCGCG
CGCCTGGATGCAGCCGGCGT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSRTLILAEQLISRPSVTPEDAGCLELLAGRLAPLGFTCERMDSGPDSFRVSNLWARRAAAGGGAPARTLVFAGHTDVVP
TGPPEQWSSPPFTPSHRDGRLYGRGASDMKTSIAAFVVAVEEFLAATPEPAIALAFLLTSDEEGPSVDGTKVVVEQLAAR
GETLDWCIVGEPTSVRKTGDMIKNGRRGTLSGKLTVRGIQGHIAYPQLARNPIHQALPALAELAATEWDRGNDFFPPTSW
QISNIHGGTGATNVIPGTVVVDFNFRFCTESTAEGLKTRVHNLLDRHGLEYDLTWTLGGQPFLTTPGELVAAVQQAITDE
TGITTELSTTGGTSDGRFIARVCPQVIELGPPNATIHKIDEHVVIADVEPLKNIYRRTLERLNAQAAAA

Sequences:

>Translated_389_residues
MSRTLILAEQLISRPSVTPEDAGCLELLAGRLAPLGFTCERMDSGPDSFRVSNLWARRAAAGGGAPARTLVFAGHTDVVP
TGPPEQWSSPPFTPSHRDGRLYGRGASDMKTSIAAFVVAVEEFLAATPEPAIALAFLLTSDEEGPSVDGTKVVVEQLAAR
GETLDWCIVGEPTSVRKTGDMIKNGRRGTLSGKLTVRGIQGHIAYPQLARNPIHQALPALAELAATEWDRGNDFFPPTSW
QISNIHGGTGATNVIPGTVVVDFNFRFCTESTAEGLKTRVHNLLDRHGLEYDLTWTLGGQPFLTTPGELVAAVQQAITDE
TGITTELSTTGGTSDGRFIARVCPQVIELGPPNATIHKIDEHVVIADVEPLKNIYRRTLERLNAQAAAA
>Mature_388_residues
SRTLILAEQLISRPSVTPEDAGCLELLAGRLAPLGFTCERMDSGPDSFRVSNLWARRAAAGGGAPARTLVFAGHTDVVPT
GPPEQWSSPPFTPSHRDGRLYGRGASDMKTSIAAFVVAVEEFLAATPEPAIALAFLLTSDEEGPSVDGTKVVVEQLAARG
ETLDWCIVGEPTSVRKTGDMIKNGRRGTLSGKLTVRGIQGHIAYPQLARNPIHQALPALAELAATEWDRGNDFFPPTSWQ
ISNIHGGTGATNVIPGTVVVDFNFRFCTESTAEGLKTRVHNLLDRHGLEYDLTWTLGGQPFLTTPGELVAAVQQAITDET
GITTELSTTGGTSDGRFIARVCPQVIELGPPNATIHKIDEHVVIADVEPLKNIYRRTLERLNAQAAAA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Homo sapiens, GI271398239, Length=397, Percent_Identity=23.6775818639798, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788816, Length=378, Percent_Identity=56.3492063492063, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1790395, Length=393, Percent_Identity=26.4631043256997, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17551016, Length=264, Percent_Identity=29.1666666666667, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_ACIAC (A1TQ19)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_970831.1
- ProteinModelPortal:   A1TQ19
- SMR:   A1TQ19
- STRING:   A1TQ19
- MEROPS:   M20.010
- GeneID:   4665199
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_2482
- NMPDR:   fig|397945.5.peg.2125
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- PhylomeDB:   A1TQ19
- ProtClustDB:   PRK13009
- BioCyc:   AAVE397945:AAVE_2482-MONOMER
- HAMAP:   MF_01690
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41631; Mature: 41500

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 77-77 ACT_SITE 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTLILAEQLISRPSVTPEDAGCLELLAGRLAPLGFTCERMDSGPDSFRVSNLWARRAA
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHCCCCCCCEEHHHHHHHHHC
AGGGAPARTLVFAGHTDVVPTGPPEQWSSPPFTPSHRDGRLYGRGASDMKTSIAAFVVAV
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHH
EEFLAATPEPAIALAFLLTSDEEGPSVDGTKVVVEQLAARGETLDWCIVGEPTSVRKTGD
HHHHHCCCCCHHEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
MIKNGRRGTLSGKLTVRGIQGHIAYPQLARNPIHQALPALAELAATEWDRGNDFFPPTSW
HHHCCCCCEECCEEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
QISNIHGGTGATNVIPGTVVVDFNFRFCTESTAEGLKTRVHNLLDRHGLEYDLTWTLGGQ
EEECCCCCCCCCCCCCCEEEEEECEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
PFLTTPGELVAAVQQAITDETGITTELSTTGGTSDGRFIARVCPQVIELGPPNATIHKID
CEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECC
EHVVIADVEPLKNIYRRTLERLNAQAAAA
CEEEEECCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRTLILAEQLISRPSVTPEDAGCLELLAGRLAPLGFTCERMDSGPDSFRVSNLWARRAA
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHCCCCCCCEEHHHHHHHHHC
AGGGAPARTLVFAGHTDVVPTGPPEQWSSPPFTPSHRDGRLYGRGASDMKTSIAAFVVAV
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHH
EEFLAATPEPAIALAFLLTSDEEGPSVDGTKVVVEQLAARGETLDWCIVGEPTSVRKTGD
HHHHHCCCCCHHEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
MIKNGRRGTLSGKLTVRGIQGHIAYPQLARNPIHQALPALAELAATEWDRGNDFFPPTSW
HHHCCCCCEECCEEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
QISNIHGGTGATNVIPGTVVVDFNFRFCTESTAEGLKTRVHNLLDRHGLEYDLTWTLGGQ
EEECCCCCCCCCCCCCCEEEEEECEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
PFLTTPGELVAAVQQAITDETGITTELSTTGGTSDGRFIARVCPQVIELGPPNATIHKID
CEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECC
EHVVIADVEPLKNIYRRTLERLNAQAAAA
CEEEEECCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA