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The map label for this gene is rlmL [H]

Identifier: 120611139

GI number: 120611139

Start: 2698392

End: 2699324

Strand: Direct

Name: rlmL [H]

Synonym: Aave_2468

Alternate gene names: 120611139

Gene position: 2698392-2699324 (Clockwise)

Preceding gene: 120611137

Following gene: 120611140

Centisome position: 50.41

GC content: 70.1

Gene sequence:

>933_bases
ATGCAAGCCCTGCTGCATGCCATCGCCCAGATGGCACCCTGTCCGGATGCACGGCGTATCTTCCACGGCCGGGGCGGCCT
CCGCCCGGGTTGCGAGCACTGGTCGCTGGATGCGTATCCGCCTGCCTTCGTGCTCACGAGCTTCCTGCCGATGGAAGAGG
ACGCCCTGGCCGCCATCGGCGCGACGCTGCAGGCCCGCTGGGCGGAGATCGCTCCCGGGGAGCCGCTGTGCTGGGTGTTC
CAGTGCAGGTGCCCGGGAAAGACCGGGACGCGCCTGATGACCGGCGAGGTGCCGGACCCGCATGCGGTCACGGAGCAGGG
CGCGCGTTACCGGGTGCATGTGCTGCGGGGCCAGAACCATGGCCTGTTCCTCGACATGGCAGAGGGCCGGCGCTGGGTGC
GCGAGCACGTGGCGGCGCGCCGGGCCGCCGGCGGGCGCGGGCCGCGGGTGCTGAATCTTTTCGCCTACACCTGCGCTTTC
TCCGTCGCGGCTTTGCAGGGCGGGGCGGCGCAGGTGGTCAATGTGGACATGGGGCAGGGCGCGATCTCCATCGGGCAGCA
GAACCACCGGCTCAACGGGCTGGAGCAGGGCGCGAGCTTCCTGGCGCACGACGTGTTCACGAGTTGGGGCAAGATCGGCC
GCGGCGGACCCTATGACCTGGTCATCGCCGATCCGCCGAGCTACCAGAAGGGCAGTTTCGTCGCGACGAAGGACTACGCA
CGGCTCATGCGGCGCCTGCCCGGCCTGCTCGCACCCGGAGGGCATGCGCTGCTGTGCCTGAACGCGCCCGAACTGGGCAC
GGACTTCCTCCTCGGCCAGATGCGCGAGCAGGCGCCGGAACTGCGCTGGATGGAGCGGGTCGCGAACCCGGCGGTGTTCG
CGGACGTGGATGCCGAGCGGTCGCTCAAGGTGATGGTGTTCGCGCTGGATTGA

Upstream 100 bases:

>100_bases
AATGCTAGCGGCCCATCAAGGCACAATCGATGCCCCAGCCACTTCTGGTCCGGAACGCGCCGCGCTTTCAACGTGCGGAG
AGCGACGGTCCCCATCTTCC

Downstream 100 bases:

>100_bases
TCCCCGCCGGCCGGACCGCCGCCCCATGCGCCGTGCAGGGCCGGCGCGAGGGGAACCAGCCCGGCAGGAAAGACTCCCAT
CCGCAGGATGCCGGGGCCGC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MQALLHAIAQMAPCPDARRIFHGRGGLRPGCEHWSLDAYPPAFVLTSFLPMEEDALAAIGATLQARWAEIAPGEPLCWVF
QCRCPGKTGTRLMTGEVPDPHAVTEQGARYRVHVLRGQNHGLFLDMAEGRRWVREHVAARRAAGGRGPRVLNLFAYTCAF
SVAALQGGAAQVVNVDMGQGAISIGQQNHRLNGLEQGASFLAHDVFTSWGKIGRGGPYDLVIADPPSYQKGSFVATKDYA
RLMRRLPGLLAPGGHALLCLNAPELGTDFLLGQMREQAPELRWMERVANPAVFADVDAERSLKVMVFALD

Sequences:

>Translated_310_residues
MQALLHAIAQMAPCPDARRIFHGRGGLRPGCEHWSLDAYPPAFVLTSFLPMEEDALAAIGATLQARWAEIAPGEPLCWVF
QCRCPGKTGTRLMTGEVPDPHAVTEQGARYRVHVLRGQNHGLFLDMAEGRRWVREHVAARRAAGGRGPRVLNLFAYTCAF
SVAALQGGAAQVVNVDMGQGAISIGQQNHRLNGLEQGASFLAHDVFTSWGKIGRGGPYDLVIADPPSYQKGSFVATKDYA
RLMRRLPGLLAPGGHALLCLNAPELGTDFLLGQMREQAPELRWMERVANPAVFADVDAERSLKVMVFALD
>Mature_310_residues
MQALLHAIAQMAPCPDARRIFHGRGGLRPGCEHWSLDAYPPAFVLTSFLPMEEDALAAIGATLQARWAEIAPGEPLCWVF
QCRCPGKTGTRLMTGEVPDPHAVTEQGARYRVHVLRGQNHGLFLDMAEGRRWVREHVAARRAAGGRGPRVLNLFAYTCAF
SVAALQGGAAQVVNVDMGQGAISIGQQNHRLNGLEQGASFLAHDVFTSWGKIGRGGPYDLVIADPPSYQKGSFVATKDYA
RLMRRLPGLLAPGGHALLCLNAPELGTDFLLGQMREQAPELRWMERVANPAVFADVDAERSLKVMVFALD

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=223, Percent_Identity=30.9417040358744, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787181, Length=199, Percent_Identity=31.6582914572864, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 33730; Mature: 33730

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALLHAIAQMAPCPDARRIFHGRGGLRPGCEHWSLDAYPPAFVLTSFLPMEEDALAAIG
CHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
ATLQARWAEIAPGEPLCWVFQCRCPGKTGTRLMTGEVPDPHAVTEQGARYRVHVLRGQNH
HHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEECCCCCCHHHHCCCCEEEEEEEECCCC
GLFLDMAEGRRWVREHVAARRAAGGRGPRVLNLFAYTCAFSVAALQGGAAQVVNVDMGQG
CEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
AISIGQQNHRLNGLEQGASFLAHDVFTSWGKIGRGGPYDLVIADPPSYQKGSFVATKDYA
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEHHHHH
RLMRRLPGLLAPGGHALLCLNAPELGTDFLLGQMREQAPELRWMERVANPAVFADVDAER
HHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCEEEECCCCC
SLKVMVFALD
CEEEEEEEEC
>Mature Secondary Structure
MQALLHAIAQMAPCPDARRIFHGRGGLRPGCEHWSLDAYPPAFVLTSFLPMEEDALAAIG
CHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
ATLQARWAEIAPGEPLCWVFQCRCPGKTGTRLMTGEVPDPHAVTEQGARYRVHVLRGQNH
HHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEECCCCCCHHHHCCCCEEEEEEEECCCC
GLFLDMAEGRRWVREHVAARRAAGGRGPRVLNLFAYTCAFSVAALQGGAAQVVNVDMGQG
CEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
AISIGQQNHRLNGLEQGASFLAHDVFTSWGKIGRGGPYDLVIADPPSYQKGSFVATKDYA
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEHHHHH
RLMRRLPGLLAPGGHALLCLNAPELGTDFLLGQMREQAPELRWMERVANPAVFADVDAER
HHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCEEEECCCCC
SLKVMVFALD
CEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA