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The map label for this gene is acyI [H]

Identifier: 120610985

GI number: 120610985

Start: 2540921

End: 2542561

Strand: Direct

Name: acyI [H]

Synonym: Aave_2313

Alternate gene names: 120610985

Gene position: 2540921-2542561 (Clockwise)

Preceding gene: 120610984

Following gene: 120610992

Centisome position: 47.47

GC content: 69.84

Gene sequence:

>1641_bases
ATGTCCCTGAACAAGCAAGCCATGGTGTCCTGCCCGCAGCCCGAAGCGGCCGAATCGGGCATCGAGATCCTGCGCGCGGG
CGGCAACGCGGTGGACGCCGCCGTCGCCACCGCGCTGGCCCAGACCGTGGTGGACCCGCTGATGTGCGGCATCGCCGGTT
TCGGCACGGCGGCCGTCTACATGCAGGGCGCCAACGGGCCGGCGGCGCATGAATACTTCGACTTCCACGCACCCGCGCCG
CTCGCCTGCCGCGAAGACATGTGGGCCGGCCTGCTCGAAGGCGAGACCCGGGACGGCTTCGGCTTCATCCTGAAGGGCCG
CGTCAACGACATCGGCCCGCAGTCCATCGCCACGCCGGCCACGCTCAAGGGGCTGGAGGCCATGCACGGCGCCCATGGCC
GCCTGCCGTGGAAGGATGTGGTCGCGCCTGCCATCGCGTGGGCGCGCGACGGCTTCTTCGTGCGGCCCGGCATGCATGCC
TTCTGGATCGACGAGCCCACCATGGGCCGCGTAAGCAACCTCGACCGCCTGCGCTACAGCGACGAAGGCCGCGCGCTCTA
CTGCCGTCCGGACGGCTCGCCCAAGCCCATCGGCACGCCGCTCGCCAATCCCGGGATGGCGGACGTGCTGGAGCAGGTGG
CGCAGGAGGGCATCGCCTCCTTCTACGAAGGCGAACTCGCGCGCCGAATGGTGGAGCATGTGCAGTCGCTGGGTGGCCTG
CTGTCGCTCGACGACCTGCGGCGCTATGCCGTGCGCCGCGTGGCGCCGCTGGTGGGAAGCTACCGCGGCCGCTCCGTCAC
CACCAACCAGCCTCCGGGCGGCGGTGCCATGCTGCTGCAGATGCTGAACATCCTGGAGAACTTCCCGCTCGCGGAGATGG
AGCACAACGGCCCGGCCTACCTGCGCCTGCTCAGCGAGGCGATGAAGAAGGCCACATCGGACAAGGACCGCCACATCGGT
GATCCGGCCTTCGTGGAGGTGCCGCTTCAGGAACTGCTCTCCAAGGACGGCGCGGCGCGCGTGGCCGAGGCGATCCGGGC
GGGCGAGCGCTTCCGCGTGGAGCGCGTGAACCCGGGGGCGCCCGTGCCGCGCGACACGACGCACCTGAGCGTGGTGGACG
GCGACGGCAACTGCGTGTCGATGGCGCATTCGCTGGCCATGCCCTCGGGCGTGATCACGCCGGGCATGGGCTTTTTCTAC
AACGGCTGCATGGGCGTGTTCGATCCGCGGCCGGGCCGCGCGGGCAGCATCCAGCCGGGCAAGAGCCGTTTCACGTCGTC
GTGCCCGAGCATCGTCTTGCAGGACGGCCGGCCGGAAATCGTACTGGGTGCCCCGGGCGGCACGCAGATCGCGATGGGCG
TGCTGCAGGTGCTGCTCAACATGATCGACCATGGCATGGGCATGCAGGAGGCCGTCTCGGCGCCGCGCTTCTCGTCCACC
AGCAATTCCATCGACGTGTGCAACCGCATTCCGCGCCACGTCACGCGCGCATTGCAGGCGGACGGCTACGAGGTGTTCCG
CAACCCGTACAACTACACGATCGGCTGGGTGCACGGCGTGCAGGTCGGGGCGGATGGCCGGCTGCACGGCGGTGCCGATC
CGGGGCGCGACGGGGTGGCCTACAGGACGTCGGTGGACTGA

Upstream 100 bases:

>100_bases
CCGGCCCCGCCAGCCAGCTCATGGAGGTGGCGCGCCGCGACACCCAGGCCTGGGGTCCGGCGCTCGACGCGCTGAACCTG
AGCGGCAAGTGAGGTCTGAG

Downstream 100 bases:

>100_bases
GAGGCCGACCGGCGGAAGAATGCGTCCAGCATGGGCATCAGCGTCGGGAATTCCTGCGCGAAGCGTTCGCGGCTGACGAA
ATAGGCCTCGCAGGCCACGG

Product: gamma-glutamyltransferase 1

Products: NA

Alternate protein names: Cephalosporin acylase; GL-7ACA acylase; Gamma-glutamyltranspeptidase; GGT [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSLNKQAMVSCPQPEAAESGIEILRAGGNAVDAAVATALAQTVVDPLMCGIAGFGTAAVYMQGANGPAAHEYFDFHAPAP
LACREDMWAGLLEGETRDGFGFILKGRVNDIGPQSIATPATLKGLEAMHGAHGRLPWKDVVAPAIAWARDGFFVRPGMHA
FWIDEPTMGRVSNLDRLRYSDEGRALYCRPDGSPKPIGTPLANPGMADVLEQVAQEGIASFYEGELARRMVEHVQSLGGL
LSLDDLRRYAVRRVAPLVGSYRGRSVTTNQPPGGGAMLLQMLNILENFPLAEMEHNGPAYLRLLSEAMKKATSDKDRHIG
DPAFVEVPLQELLSKDGAARVAEAIRAGERFRVERVNPGAPVPRDTTHLSVVDGDGNCVSMAHSLAMPSGVITPGMGFFY
NGCMGVFDPRPGRAGSIQPGKSRFTSSCPSIVLQDGRPEIVLGAPGGTQIAMGVLQVLLNMIDHGMGMQEAVSAPRFSST
SNSIDVCNRIPRHVTRALQADGYEVFRNPYNYTIGWVHGVQVGADGRLHGGADPGRDGVAYRTSVD

Sequences:

>Translated_546_residues
MSLNKQAMVSCPQPEAAESGIEILRAGGNAVDAAVATALAQTVVDPLMCGIAGFGTAAVYMQGANGPAAHEYFDFHAPAP
LACREDMWAGLLEGETRDGFGFILKGRVNDIGPQSIATPATLKGLEAMHGAHGRLPWKDVVAPAIAWARDGFFVRPGMHA
FWIDEPTMGRVSNLDRLRYSDEGRALYCRPDGSPKPIGTPLANPGMADVLEQVAQEGIASFYEGELARRMVEHVQSLGGL
LSLDDLRRYAVRRVAPLVGSYRGRSVTTNQPPGGGAMLLQMLNILENFPLAEMEHNGPAYLRLLSEAMKKATSDKDRHIG
DPAFVEVPLQELLSKDGAARVAEAIRAGERFRVERVNPGAPVPRDTTHLSVVDGDGNCVSMAHSLAMPSGVITPGMGFFY
NGCMGVFDPRPGRAGSIQPGKSRFTSSCPSIVLQDGRPEIVLGAPGGTQIAMGVLQVLLNMIDHGMGMQEAVSAPRFSST
SNSIDVCNRIPRHVTRALQADGYEVFRNPYNYTIGWVHGVQVGADGRLHGGADPGRDGVAYRTSVD
>Mature_545_residues
SLNKQAMVSCPQPEAAESGIEILRAGGNAVDAAVATALAQTVVDPLMCGIAGFGTAAVYMQGANGPAAHEYFDFHAPAPL
ACREDMWAGLLEGETRDGFGFILKGRVNDIGPQSIATPATLKGLEAMHGAHGRLPWKDVVAPAIAWARDGFFVRPGMHAF
WIDEPTMGRVSNLDRLRYSDEGRALYCRPDGSPKPIGTPLANPGMADVLEQVAQEGIASFYEGELARRMVEHVQSLGGLL
SLDDLRRYAVRRVAPLVGSYRGRSVTTNQPPGGGAMLLQMLNILENFPLAEMEHNGPAYLRLLSEAMKKATSDKDRHIGD
PAFVEVPLQELLSKDGAARVAEAIRAGERFRVERVNPGAPVPRDTTHLSVVDGDGNCVSMAHSLAMPSGVITPGMGFFYN
GCMGVFDPRPGRAGSIQPGKSRFTSSCPSIVLQDGRPEIVLGAPGGTQIAMGVLQVLLNMIDHGMGMQEAVSAPRFSSTS
NSIDVCNRIPRHVTRALQADGYEVFRNPYNYTIGWVHGVQVGADGRLHGGADPGRDGVAYRTSVD

Specific function: Besides the cephalosporin acylase I activity which converts GL-7ACA into 7-ACA; this enzyme displays some gamma glutamyltranspeptidase activity [H]

COG id: COG0405

COG function: function code E; Gamma-glutamyltransferase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI73915092, Length=521, Percent_Identity=28.7907869481766, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI73915090, Length=521, Percent_Identity=28.7907869481766, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI73915096, Length=521, Percent_Identity=28.7907869481766, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI73915094, Length=521, Percent_Identity=28.7907869481766, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI113429324, Length=518, Percent_Identity=29.1505791505791, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI113429326, Length=519, Percent_Identity=28.9017341040462, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI109148539, Length=530, Percent_Identity=29.811320754717, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI153266885, Length=539, Percent_Identity=25.6029684601113, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI153266901, Length=540, Percent_Identity=25.5555555555556, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI153266907, Length=452, Percent_Identity=26.1061946902655, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI30581157, Length=164, Percent_Identity=33.5365853658537, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI30581155, Length=164, Percent_Identity=33.5365853658537, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789856, Length=554, Percent_Identity=31.7689530685921, Blast_Score=258, Evalue=9e-70,
Organism=Caenorhabditis elegans, GI17542894, Length=552, Percent_Identity=25.9057971014493, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17506189, Length=567, Percent_Identity=24.8677248677249, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI72000294, Length=556, Percent_Identity=23.7410071942446, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI212646519, Length=556, Percent_Identity=23.7410071942446, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI193208245, Length=514, Percent_Identity=26.2645914396887, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI193208247, Length=514, Percent_Identity=26.2645914396887, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323330, Length=488, Percent_Identity=25.8196721311475, Blast_Score=113, Evalue=7e-26,
Organism=Drosophila melanogaster, GI161077496, Length=514, Percent_Identity=28.0155642023346, Blast_Score=155, Evalue=8e-38,
Organism=Drosophila melanogaster, GI18859893, Length=525, Percent_Identity=27.2380952380952, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI161077878, Length=496, Percent_Identity=26.2096774193548, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24642670, Length=496, Percent_Identity=26.2096774193548, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24642666, Length=496, Percent_Identity=26.2096774193548, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI161077880, Length=496, Percent_Identity=26.2096774193548, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI45551474, Length=496, Percent_Identity=26.2096774193548, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI18859741, Length=482, Percent_Identity=27.8008298755187, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000101 [H]

Pfam domain/function: PF01019 G_glu_transpept [H]

EC number: =2.3.2.2 [H]

Molecular weight: Translated: 58322; Mature: 58191

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNKQAMVSCPQPEAAESGIEILRAGGNAVDAAVATALAQTVVDPLMCGIAGFGTAAVY
CCCCCCHHEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
MQGANGPAAHEYFDFHAPAPLACREDMWAGLLEGETRDGFGFILKGRVNDIGPQSIATPA
EECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCH
TLKGLEAMHGAHGRLPWKDVVAPAIAWARDGFFVRPGMHAFWIDEPTMGRVSNLDRLRYS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCHHHCHHHHEEC
DEGRALYCRPDGSPKPIGTPLANPGMADVLEQVAQEGIASFYEGELARRMVEHVQSLGGL
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSLDDLRRYAVRRVAPLVGSYRGRSVTTNQPPGGGAMLLQMLNILENFPLAEMEHNGPAY
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
LRLLSEAMKKATSDKDRHIGDPAFVEVPLQELLSKDGAARVAEAIRAGERFRVERVNPGA
HHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCC
PVPRDTTHLSVVDGDGNCVSMAHSLAMPSGVITPGMGFFYNGCMGVFDPRPGRAGSIQPG
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCC
KSRFTSSCPSIVLQDGRPEIVLGAPGGTQIAMGVLQVLLNMIDHGMGMQEAVSAPRFSST
HHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCC
SNSIDVCNRIPRHVTRALQADGYEVFRNPYNYTIGWVHGVQVGADGRLHGGADPGRDGVA
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCE
YRTSVD
EECCCC
>Mature Secondary Structure 
SLNKQAMVSCPQPEAAESGIEILRAGGNAVDAAVATALAQTVVDPLMCGIAGFGTAAVY
CCCCCHHEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
MQGANGPAAHEYFDFHAPAPLACREDMWAGLLEGETRDGFGFILKGRVNDIGPQSIATPA
EECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCH
TLKGLEAMHGAHGRLPWKDVVAPAIAWARDGFFVRPGMHAFWIDEPTMGRVSNLDRLRYS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCHHHCHHHHEEC
DEGRALYCRPDGSPKPIGTPLANPGMADVLEQVAQEGIASFYEGELARRMVEHVQSLGGL
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSLDDLRRYAVRRVAPLVGSYRGRSVTTNQPPGGGAMLLQMLNILENFPLAEMEHNGPAY
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
LRLLSEAMKKATSDKDRHIGDPAFVEVPLQELLSKDGAARVAEAIRAGERFRVERVNPGA
HHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCC
PVPRDTTHLSVVDGDGNCVSMAHSLAMPSGVITPGMGFFYNGCMGVFDPRPGRAGSIQPG
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCC
KSRFTSSCPSIVLQDGRPEIVLGAPGGTQIAMGVLQVLLNMIDHGMGMQEAVSAPRFSST
HHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCC
SNSIDVCNRIPRHVTRALQADGYEVFRNPYNYTIGWVHGVQVGADGRLHGGADPGRDGVA
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCE
YRTSVD
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3680178 [H]