Click here to switch to the map view.

The map label for this gene is cysS

Identifier: 120610992

GI number: 120610992

Start: 2548279

End: 2549658

Strand: Direct

Name: cysS

Synonym: Aave_2320

Alternate gene names: 120610992

Gene position: 2548279-2549658 (Clockwise)

Preceding gene: 120610985

Following gene: 120610993

Centisome position: 47.61

GC content: 68.7

Gene sequence:

>1380_bases
ATGAGTTTGCGCATCTACAACACGCTGTCGCGTGCGATGGAGGACTTTTCCCCGCTCGAACCCGGCCATGTCCGCATGTA
CGTCTGCGGCATGACCATCTACGACCTCTGCCACATCGGCCACGCCCGCATGATGATGGCGTTCGACGTGGTGCAGCGCT
GGCTCCGCAGCAGCGGCTTGCGCGTTACCTATGTGCGCAACATCACCGACATCGACGACAAGATCATCCGCCGCGCGGTG
GAGCGGGGCATCACCATCCGCCAGCTCACGGACGAGATGACGGCCGCCATGCACGAGGACATCGGCGCCCTGGCGATCGA
GCCGCCCACGGTCGAGCCGCGCGCCACCGAATACGTGCCCCAGATGCTCTCGCTCATCGGCACGCTGGAGCGCAAGGGCC
TGGCCTACCGCGCGGGCAGCGGCGACGTGAACTACGCCGTGCGCCGGTTCGAGGGCTACGGCAAGCTGTCGGGCAAGTCC
CTGGACGAATTGCGCGCGGGCGAACGCGTGGCCGTGCAGGACGACAAGCAGGATCCGCTCGACTTCGTGCTCTGGAAGGC
CGCCAAGCCCGGCGAGCCGCCCGAGGCGAAATGGGACAGCCCCTTCGGCACCGGCCGGCCGGGCTGGCACATCGAGTGCT
CGGCCATGAGCTGCGCCACGCTCGGCGAGAGCTTCGACATCCACGGCGGCGGGGCGGACCTGCAGTTCCCCCACCACGAG
AACGAGATCGCCCAGAGCGAGGGTGCCACCGGCAAGCCGCTGGCGCGCTTCTGGATGCACAACGGCTTCGTGCGCGTGGA
CAACGAGAAGATGAGCAAGTCGCTCGGCAACTTCTTCACCATCCGCGAGGTGCTGCAGCAGGTCGATGCCGAGACGCTGC
GCTTCTTCATCGTGCGCACGCACTACCGCAGCCCGCTCAACTACAGCGACGTGCACCTGCAGGATGCGCGCGCTTCGCTC
AAGCGGCTCTACACCGCGCTCGCCCTGGTCGCGCCGGAAGACATCGGCGCCATCGACTGGGCCGATCCCCATGCGGCCCG
TTTCAAGGCGGCCATGGACGAGGATTTCGGCACGCCCGAGGCGGTGGCCGTGCTGTTCGAGCTGGCGGGCGAGGTGAACC
GTACGCGGTCTGCCCGTCTGGCCGGCCTGCTGAAGGCACTGGGCGGCTGCCTGGGGCTGCTGCAGGGAGATCCGCAGGCC
TTCCTGCGGCAGGGGGTGCAACTGGACGCGGCCGAGATCGCCGCCCGCATCGCCGCGCGCGCGGCCGCCAAGGCAGCGCG
CGACTTCGCCGAAGCCGACCGCATCCGCAACGACCTGCTCGCGCAGGGCATCGTGCTCAAGGACTCCCCCGCGGGCACGA
CCTGGGAGGCCGCGCAGTGA

Upstream 100 bases:

>100_bases
ATGCTGCGCATACCTCGCACGCCGGCCCGTCCGGCGCCACGGTCCGCTGAGGCCGGGGCCGCCGGCCCGCAGTCCAACAC
CCCCTCTTCACTGATATTCC

Downstream 100 bases:

>100_bases
GCCCGGGCCGCCCCTTCCGTTTCCCGCACCGCCGGTCTCCCCTCCAGACAAGGTAAGCCGTGGCCCCCAGCAAGAAAACA
GCGGGGACGGCCGAGCCGTC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSLRIYNTLSRAMEDFSPLEPGHVRMYVCGMTIYDLCHIGHARMMMAFDVVQRWLRSSGLRVTYVRNITDIDDKIIRRAV
ERGITIRQLTDEMTAAMHEDIGALAIEPPTVEPRATEYVPQMLSLIGTLERKGLAYRAGSGDVNYAVRRFEGYGKLSGKS
LDELRAGERVAVQDDKQDPLDFVLWKAAKPGEPPEAKWDSPFGTGRPGWHIECSAMSCATLGESFDIHGGGADLQFPHHE
NEIAQSEGATGKPLARFWMHNGFVRVDNEKMSKSLGNFFTIREVLQQVDAETLRFFIVRTHYRSPLNYSDVHLQDARASL
KRLYTALALVAPEDIGAIDWADPHAARFKAAMDEDFGTPEAVAVLFELAGEVNRTRSARLAGLLKALGGCLGLLQGDPQA
FLRQGVQLDAAEIAARIAARAAAKAARDFAEADRIRNDLLAQGIVLKDSPAGTTWEAAQ

Sequences:

>Translated_459_residues
MSLRIYNTLSRAMEDFSPLEPGHVRMYVCGMTIYDLCHIGHARMMMAFDVVQRWLRSSGLRVTYVRNITDIDDKIIRRAV
ERGITIRQLTDEMTAAMHEDIGALAIEPPTVEPRATEYVPQMLSLIGTLERKGLAYRAGSGDVNYAVRRFEGYGKLSGKS
LDELRAGERVAVQDDKQDPLDFVLWKAAKPGEPPEAKWDSPFGTGRPGWHIECSAMSCATLGESFDIHGGGADLQFPHHE
NEIAQSEGATGKPLARFWMHNGFVRVDNEKMSKSLGNFFTIREVLQQVDAETLRFFIVRTHYRSPLNYSDVHLQDARASL
KRLYTALALVAPEDIGAIDWADPHAARFKAAMDEDFGTPEAVAVLFELAGEVNRTRSARLAGLLKALGGCLGLLQGDPQA
FLRQGVQLDAAEIAARIAARAAAKAARDFAEADRIRNDLLAQGIVLKDSPAGTTWEAAQ
>Mature_458_residues
SLRIYNTLSRAMEDFSPLEPGHVRMYVCGMTIYDLCHIGHARMMMAFDVVQRWLRSSGLRVTYVRNITDIDDKIIRRAVE
RGITIRQLTDEMTAAMHEDIGALAIEPPTVEPRATEYVPQMLSLIGTLERKGLAYRAGSGDVNYAVRRFEGYGKLSGKSL
DELRAGERVAVQDDKQDPLDFVLWKAAKPGEPPEAKWDSPFGTGRPGWHIECSAMSCATLGESFDIHGGGADLQFPHHEN
EIAQSEGATGKPLARFWMHNGFVRVDNEKMSKSLGNFFTIREVLQQVDAETLRFFIVRTHYRSPLNYSDVHLQDARASLK
RLYTALALVAPEDIGAIDWADPHAARFKAAMDEDFGTPEAVAVLFELAGEVNRTRSARLAGLLKALGGCLGLLQGDPQAF
LRQGVQLDAAEIAARIAARAAAKAARDFAEADRIRNDLLAQGIVLKDSPAGTTWEAAQ

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=399, Percent_Identity=38.0952380952381, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI303304987, Length=234, Percent_Identity=39.7435897435897, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI21269877, Length=234, Percent_Identity=39.7435897435897, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI62240992, Length=234, Percent_Identity=39.7435897435897, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI10835051, Length=234, Percent_Identity=39.7435897435897, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI1786737, Length=466, Percent_Identity=54.2918454935622, Blast_Score=476, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17509797, Length=236, Percent_Identity=38.5593220338983, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17509795, Length=236, Percent_Identity=38.5593220338983, Blast_Score=165, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324082, Length=258, Percent_Identity=34.8837209302326, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI19922218, Length=506, Percent_Identity=31.6205533596838, Blast_Score=223, Evalue=3e-58,
Organism=Drosophila melanogaster, GI19922364, Length=227, Percent_Identity=43.6123348017621, Blast_Score=178, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_ACIAC (A1TPK8)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_970670.1
- ProteinModelPortal:   A1TPK8
- SMR:   A1TPK8
- STRING:   A1TPK8
- GeneID:   4667407
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_2320
- NMPDR:   fig|397945.5.peg.2004
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   A1TPK8
- ProtClustDB:   PRK00260
- BioCyc:   AAVE397945:AAVE_2320-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 50742; Mature: 50611

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRIYNTLSRAMEDFSPLEPGHVRMYVCGMTIYDLCHIGHARMMMAFDVVQRWLRSSGL
CCEEHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RVTYVRNITDIDDKIIRRAVERGITIRQLTDEMTAAMHEDIGALAIEPPTVEPRATEYVP
EEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHH
QMLSLIGTLERKGLAYRAGSGDVNYAVRRFEGYGKLSGKSLDELRAGERVAVQDDKQDPL
HHHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCEEEECCCCCCCH
DFVLWKAAKPGEPPEAKWDSPFGTGRPGWHIECSAMSCATLGESFDIHGGGADLQFPHHE
HHEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCCCEECCCCCCCCCCCCC
NEIAQSEGATGKPLARFWMHNGFVRVDNEKMSKSLGNFFTIREVLQQVDAETLRFFIVRT
HHHHHCCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
HYRSPLNYSDVHLQDARASLKRLYTALALVAPEDIGAIDWADPHAARFKAAMDEDFGTPE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHH
AVAVLFELAGEVNRTRSARLAGLLKALGGCLGLLQGDPQAFLRQGVQLDAAEIAARIAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHH
AAAKAARDFAEADRIRNDLLAQGIVLKDSPAGTTWEAAQ
HHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SLRIYNTLSRAMEDFSPLEPGHVRMYVCGMTIYDLCHIGHARMMMAFDVVQRWLRSSGL
CEEHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RVTYVRNITDIDDKIIRRAVERGITIRQLTDEMTAAMHEDIGALAIEPPTVEPRATEYVP
EEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHH
QMLSLIGTLERKGLAYRAGSGDVNYAVRRFEGYGKLSGKSLDELRAGERVAVQDDKQDPL
HHHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCEEEECCCCCCCH
DFVLWKAAKPGEPPEAKWDSPFGTGRPGWHIECSAMSCATLGESFDIHGGGADLQFPHHE
HHEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCCCEECCCCCCCCCCCCC
NEIAQSEGATGKPLARFWMHNGFVRVDNEKMSKSLGNFFTIREVLQQVDAETLRFFIVRT
HHHHHCCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
HYRSPLNYSDVHLQDARASLKRLYTALALVAPEDIGAIDWADPHAARFKAAMDEDFGTPE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHH
AVAVLFELAGEVNRTRSARLAGLLKALGGCLGLLQGDPQAFLRQGVQLDAAEIAARIAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHH
AAAKAARDFAEADRIRNDLLAQGIVLKDSPAGTTWEAAQ
HHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA