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The map label for this gene is fumB [H]

Identifier: 120610175

GI number: 120610175

Start: 1649597

End: 1651156

Strand: Reverse

Name: fumB [H]

Synonym: Aave_1489

Alternate gene names: 120610175

Gene position: 1651156-1649597 (Counterclockwise)

Preceding gene: 120610176

Following gene: 120610174

Centisome position: 30.85

GC content: 67.05

Gene sequence:

>1560_bases
ATGAGCACCACCATCCGGTACCACGACCTCGTGGAATCCGTCGCCGCGTCCCTGCAGTACATCAGCTACTACCACCCCGC
CGACTTCATCGCCCACCTGGCCCGCGCCTACGAGCGCGAGCAGAGCCCGGCGGCCAGGGACGCGATGGCGCAGATCCTCA
CCAACAGCAAGATGAGCGCCACCGGCCAGCGCCCCATCTGCCAGGACACCGGCATCGTCAACGTGTTCCTGAAGATCGGC
ATGGACGTGCGCTGGGAGGGCTTCCCGGCCGGCCAGGGCCTGGAGGACGCCGTCAACGAAGGCGTGCGCCGCGGCTACAA
CCATCCCGACAACACGCTGCGCGCGAGCGTCGTGGCCGATCCGCAGTTCCTGCGCAAGAACACGAAGGACAACACCCCTG
CCGTGATCTTCACGCAGATCGTGCCCGGCAACACCGTGGACATCACCGTCGCGGCCAAGGGCGGCGGCAGCGAGAACAAG
TCCAAGATGGTCATGATGAACCCCAGCGACAACCTGGTGGACTGGGTGCTCAAGACCGTGCCCACCATGGGCGCCGGCTG
GTGCCCTCCGGGTATGCTGGGCATCGGCATCGGCGGCACGGCGGAGAAGGCCGTCCTGCTGGCCAAGGAAAGCCTGATGG
ACGACCTGAACATGTACGAGCTGCAGGCCAAGGCCGCGCGCGGCGAGAAGCTCGACCAGGTGGAGGAGTTGCGCCTGGAG
CTGTTCGACAAGGTCAACGCCCTGGGCATCGGCGCGCAGGGCCTGGGCGGCCTCACCACCGTGCTGGACATCAAGATCAA
GATGTATCCCACGCATGCGGCCTCCAAGCCGGTCGCCATGATCCCGAACTGCGCCGCCACGCGCCATGCGCACTTCGTGC
TGGACGGCTCCGGCCCCGTGTACCTGGAGGCACCGTCGCTGGACCTGTGGCCGAAGATCGACTGGGCCCCCGACTACAAC
AAGAGCCGCCGCGTCAGCCTCGATACGCTGACCAGGGAGGAGGTGGCCAGCTGGAAGCCCGGCGACACGCTGCTGCTCAA
CGGCAAGATGCTCACCGGCCGCGACGCCGCGCACAAGCGCATCCAGGACATGCTGGCCAAGGGCGAGCCGCTGCCCGTGG
ACTTCACCAACCGCGTGATCTACTACGTCGGCCCGGTCGATCCGGTGCGCGACGAGGCCGTAGGCCCCGCCGGCCCGACC
ACGGCGACACGCATGGACAAGTTCACCGACATGATGCTCGCGCAGACCGGCCTGATCGCGATGATCGGCAAGTCCGAGCG
CGGCCCCGTCGCCATCGAGGCGATCAAGAAGCACCAGTCGGCCTACCTGATGGCCGTGGGCGGCGCCGCCTACCTGGTCA
GCAAAGCCATCAAGACCGCGAAGGTGGTCGGCTTCGAAGACCTGGGGATGGAAGCGATCTACGAATTCGACGTGGTGGAC
ATGCCCGTGACGGTGGCCGTGGACGCCGGCGGCACCAGCGCCCACATCACGGGCCCGGCCGAATGGCAGAAGCGCATCGC
CACTGGCGAGTTCAAGTCCATCGACGTCGTGGCGGCCTGA

Upstream 100 bases:

>100_bases
AGTGGACGCACCGGCCTGATGTTCCACGGTCGGCAAGCACCTCGCCGGGGCGCGGCGCGATAATCATGGCCGTCAGTCCT
ACCTACAACGCATCCCCACC

Downstream 100 bases:

>100_bases
CGCGCGTGCGCGCTCCGGCGCGCCCTGCACTACCATTCGGGGCCCGCGGCGCACACAGGGCGCGCCGCAGGCCCCTTTCA
CATTCGCCCCTCCGACATGA

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSTTIRYHDLVESVAASLQYISYYHPADFIAHLARAYEREQSPAARDAMAQILTNSKMSATGQRPICQDTGIVNVFLKIG
MDVRWEGFPAGQGLEDAVNEGVRRGYNHPDNTLRASVVADPQFLRKNTKDNTPAVIFTQIVPGNTVDITVAAKGGGSENK
SKMVMMNPSDNLVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAVLLAKESLMDDLNMYELQAKAARGEKLDQVEELRLE
LFDKVNALGIGAQGLGGLTTVLDIKIKMYPTHAASKPVAMIPNCAATRHAHFVLDGSGPVYLEAPSLDLWPKIDWAPDYN
KSRRVSLDTLTREEVASWKPGDTLLLNGKMLTGRDAAHKRIQDMLAKGEPLPVDFTNRVIYYVGPVDPVRDEAVGPAGPT
TATRMDKFTDMMLAQTGLIAMIGKSERGPVAIEAIKKHQSAYLMAVGGAAYLVSKAIKTAKVVGFEDLGMEAIYEFDVVD
MPVTVAVDAGGTSAHITGPAEWQKRIATGEFKSIDVVAA

Sequences:

>Translated_519_residues
MSTTIRYHDLVESVAASLQYISYYHPADFIAHLARAYEREQSPAARDAMAQILTNSKMSATGQRPICQDTGIVNVFLKIG
MDVRWEGFPAGQGLEDAVNEGVRRGYNHPDNTLRASVVADPQFLRKNTKDNTPAVIFTQIVPGNTVDITVAAKGGGSENK
SKMVMMNPSDNLVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAVLLAKESLMDDLNMYELQAKAARGEKLDQVEELRLE
LFDKVNALGIGAQGLGGLTTVLDIKIKMYPTHAASKPVAMIPNCAATRHAHFVLDGSGPVYLEAPSLDLWPKIDWAPDYN
KSRRVSLDTLTREEVASWKPGDTLLLNGKMLTGRDAAHKRIQDMLAKGEPLPVDFTNRVIYYVGPVDPVRDEAVGPAGPT
TATRMDKFTDMMLAQTGLIAMIGKSERGPVAIEAIKKHQSAYLMAVGGAAYLVSKAIKTAKVVGFEDLGMEAIYEFDVVD
MPVTVAVDAGGTSAHITGPAEWQKRIATGEFKSIDVVAA
>Mature_518_residues
STTIRYHDLVESVAASLQYISYYHPADFIAHLARAYEREQSPAARDAMAQILTNSKMSATGQRPICQDTGIVNVFLKIGM
DVRWEGFPAGQGLEDAVNEGVRRGYNHPDNTLRASVVADPQFLRKNTKDNTPAVIFTQIVPGNTVDITVAAKGGGSENKS
KMVMMNPSDNLVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAVLLAKESLMDDLNMYELQAKAARGEKLDQVEELRLEL
FDKVNALGIGAQGLGGLTTVLDIKIKMYPTHAASKPVAMIPNCAATRHAHFVLDGSGPVYLEAPSLDLWPKIDWAPDYNK
SRRVSLDTLTREEVASWKPGDTLLLNGKMLTGRDAAHKRIQDMLAKGEPLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTT
ATRMDKFTDMMLAQTGLIAMIGKSERGPVAIEAIKKHQSAYLMAVGGAAYLVSKAIKTAKVVGFEDLGMEAIYEFDVVDM
PVTVAVDAGGTSAHITGPAEWQKRIATGEFKSIDVVAA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=496, Percent_Identity=32.6612903225806, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1790564, Length=507, Percent_Identity=30.7692307692308, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1789442, Length=290, Percent_Identity=25.5172413793103, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789443, Length=167, Percent_Identity=28.1437125748503, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 56073; Mature: 55942

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTIRYHDLVESVAASLQYISYYHPADFIAHLARAYEREQSPAARDAMAQILTNSKMSA
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
TGQRPICQDTGIVNVFLKIGMDVRWEGFPAGQGLEDAVNEGVRRGYNHPDNTLRASVVAD
CCCCCCCCCCCHHEEHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECC
PQFLRKNTKDNTPAVIFTQIVPGNTVDITVAAKGGGSENKSKMVMMNPSDNLVDWVLKTV
HHHHHCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHH
PTMGAGWCPPGMLGIGIGGTAEKAVLLAKESLMDDLNMYELQAKAARGEKLDQVEELRLE
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHH
LFDKVNALGIGAQGLGGLTTVLDIKIKMYPTHAASKPVAMIPNCAATRHAHFVLDGSGPV
HHHHHHHEEECCCCCCCEEEEEEEEEEEEECCCCCCCEEECCCCCCCCCEEEEEECCCEE
YLEAPSLDLWPKIDWAPDYNKSRRVSLDTLTREEVASWKPGDTLLLNGKMLTGRDAAHKR
EEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEECCEEECCCHHHHHH
IQDMLAKGEPLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKFTDMMLAQTGLIA
HHHHHHCCCCCCEECCCCEEEEECCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEE
MIGKSERGPVAIEAIKKHQSAYLMAVGGAAYLVSKAIKTAKVVGFEDLGMEAIYEFDVVD
EECCCCCCCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHEECHHHCCHHHEEEEEEEC
MPVTVAVDAGGTSAHITGPAEWQKRIATGEFKSIDVVAA
CCEEEEEECCCCEEEECCHHHHHHHHCCCCCCCEEEEEC
>Mature Secondary Structure 
STTIRYHDLVESVAASLQYISYYHPADFIAHLARAYEREQSPAARDAMAQILTNSKMSA
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
TGQRPICQDTGIVNVFLKIGMDVRWEGFPAGQGLEDAVNEGVRRGYNHPDNTLRASVVAD
CCCCCCCCCCCHHEEHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECC
PQFLRKNTKDNTPAVIFTQIVPGNTVDITVAAKGGGSENKSKMVMMNPSDNLVDWVLKTV
HHHHHCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHH
PTMGAGWCPPGMLGIGIGGTAEKAVLLAKESLMDDLNMYELQAKAARGEKLDQVEELRLE
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHH
LFDKVNALGIGAQGLGGLTTVLDIKIKMYPTHAASKPVAMIPNCAATRHAHFVLDGSGPV
HHHHHHHEEECCCCCCCEEEEEEEEEEEEECCCCCCCEEECCCCCCCCCEEEEEECCCEE
YLEAPSLDLWPKIDWAPDYNKSRRVSLDTLTREEVASWKPGDTLLLNGKMLTGRDAAHKR
EEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEECCEEECCCHHHHHH
IQDMLAKGEPLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKFTDMMLAQTGLIA
HHHHHHCCCCCCEECCCCEEEEECCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEE
MIGKSERGPVAIEAIKKHQSAYLMAVGGAAYLVSKAIKTAKVVGFEDLGMEAIYEFDVVD
EECCCCCCCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHEECHHHCCHHHEEEEEEEC
MPVTVAVDAGGTSAHITGPAEWQKRIATGEFKSIDVVAA
CCEEEEEECCCCEEEECCHHHHHHHHCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]