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The map label for this gene is ispG [H]

Identifier: 120610111

GI number: 120610111

Start: 1564336

End: 1565616

Strand: Direct

Name: ispG [H]

Synonym: Aave_1424

Alternate gene names: 120610111

Gene position: 1564336-1565616 (Clockwise)

Preceding gene: 120610110

Following gene: 120610112

Centisome position: 29.22

GC content: 67.76

Gene sequence:

>1281_bases
GTGATCCACACTCCCAATGCCGAATCCCCCATCGCCATGGCATCGCCGCTGCCGCGGCGGTCGCGCCAGGCACGCATCGC
CTGGGGCGACCGGGTGGTGACGGTCGGCGGCGACGCGCCCGTGCGCGTGCAGTCCATGACCAACACCGACACCGTGGATG
CAATCGCCACGGCCATCCAGGTCAAGGAACTGGCGCAGGCAGGTTCCGAGTTCGTGCGCATCACCGTCAACACGCCCGAG
GCGGCAGCCGCCGTTCCGTACATCCGCGAGCAGCTCGACCGCATGGGCGAGACCGTGCCGCTGGTGGGCGATTTCCACTA
CAACGGACACCGCCTGCTCACCGAATACCCGGATTGCGCCCAGGCGCTTTCCAAGTACCGCATCAACCCCGGCAATGTCG
GCAAGGGCGACAAACGCGACCGGCAGTTCGGCCAGATGATCGAGGCGGCCATGCGGTGGAACAAGGCCGTGCGCATCGGC
GTGAACTGGGGCAGCCTCGACCAGGAACTGCTCGCCGGCCTGATGGACGCTAACAGCCGGCGTGCCGTGCCCTGGGATGC
CCGGCAGGTCATGTACGAGGCCCTGATCACCTCCGCCATCGAATCGGCGCAGCGGGCCGAGGCCATGGGGCTCGATGGCA
ACCAGATCATCCTGTCCTGCAAGGTCAGCGGCGTGCAGGATCTCATCGCCGTGTACCGCGAACTCGCCCGCCGCTGCGAC
TACGCCCTGCACCTGGGCCTGACCGAGGCGGGCATGGGCACCAAGGGAACGGTCGCCTCCGCGGCTGCGCTGTCGGTGCT
GCTGCAGGAGGGCATCGGGGACACCATCCGGGTGTCGCTCACGCCCCAGCCCGGCGAGGCCCGTACCCAGGAAGTCATCG
TGGCTTCCGAGATCCTGCAGGCCCTGGGCCTGCGCGTCTTCGTGCCCAGCGTCACCGCCTGCCCGGGCTGCGGGCGCACC
ACCAGCACCACCTTCCAGGAACTGGCCAAGCAGATCGACGACTTCCTGCGCTCGCAGATGCCCGTCTGGCGCACCCGCTA
TCCTGGCGTCGAGAAAATGAAGGTGGCCGTGATGGGCTGCATCGTGAACGGTCCCGGCGAAAGCAAGCACGCCGACATCG
GCATCAGCCTGCCCGGAACGGGCGAGGCCCCGGCCGCGCCGGTATTCATCGATGGAGAGAAGGCGATGACCCTGCGGGGA
GAGCACATCGCCCGCGACTTCCAGCAGGTGGTCGAGGACTATATTTCCCGCCGCTTCGGCAGCACCGCCGAGGTTCTCTG
A

Upstream 100 bases:

>100_bases
GGTCGGCAAGGCCGACGCCACCGAGGTCCTGGTGCGTGGCCAGCCCATGGATCTGGCGCCCGTCGCGCGCGAGAACGTTG
CCCGGTTCGAGGTGAAATAA

Downstream 100 bases:

>100_bases
TCCGCTCCGGGCAGGAGCGCCGCATCCCTTCGTGATGCCGGCCATGCCCGCACCCGGGGCCCATGCCCCCACCTTCCGAC
GTATGTCCGCAAGCAATCCC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MIHTPNAESPIAMASPLPRRSRQARIAWGDRVVTVGGDAPVRVQSMTNTDTVDAIATAIQVKELAQAGSEFVRITVNTPE
AAAAVPYIREQLDRMGETVPLVGDFHYNGHRLLTEYPDCAQALSKYRINPGNVGKGDKRDRQFGQMIEAAMRWNKAVRIG
VNWGSLDQELLAGLMDANSRRAVPWDARQVMYEALITSAIESAQRAEAMGLDGNQIILSCKVSGVQDLIAVYRELARRCD
YALHLGLTEAGMGTKGTVASAAALSVLLQEGIGDTIRVSLTPQPGEARTQEVIVASEILQALGLRVFVPSVTACPGCGRT
TSTTFQELAKQIDDFLRSQMPVWRTRYPGVEKMKVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFIDGEKAMTLRG
EHIARDFQQVVEDYISRRFGSTAEVL

Sequences:

>Translated_426_residues
MIHTPNAESPIAMASPLPRRSRQARIAWGDRVVTVGGDAPVRVQSMTNTDTVDAIATAIQVKELAQAGSEFVRITVNTPE
AAAAVPYIREQLDRMGETVPLVGDFHYNGHRLLTEYPDCAQALSKYRINPGNVGKGDKRDRQFGQMIEAAMRWNKAVRIG
VNWGSLDQELLAGLMDANSRRAVPWDARQVMYEALITSAIESAQRAEAMGLDGNQIILSCKVSGVQDLIAVYRELARRCD
YALHLGLTEAGMGTKGTVASAAALSVLLQEGIGDTIRVSLTPQPGEARTQEVIVASEILQALGLRVFVPSVTACPGCGRT
TSTTFQELAKQIDDFLRSQMPVWRTRYPGVEKMKVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFIDGEKAMTLRG
EHIARDFQQVVEDYISRRFGSTAEVL
>Mature_426_residues
MIHTPNAESPIAMASPLPRRSRQARIAWGDRVVTVGGDAPVRVQSMTNTDTVDAIATAIQVKELAQAGSEFVRITVNTPE
AAAAVPYIREQLDRMGETVPLVGDFHYNGHRLLTEYPDCAQALSKYRINPGNVGKGDKRDRQFGQMIEAAMRWNKAVRIG
VNWGSLDQELLAGLMDANSRRAVPWDARQVMYEALITSAIESAQRAEAMGLDGNQIILSCKVSGVQDLIAVYRELARRCD
YALHLGLTEAGMGTKGTVASAAALSVLLQEGIGDTIRVSLTPQPGEARTQEVIVASEILQALGLRVFVPSVTACPGCGRT
TSTTFQELAKQIDDFLRSQMPVWRTRYPGVEKMKVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFIDGEKAMTLRG
EHIARDFQQVVEDYISRRFGSTAEVL

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=385, Percent_Identity=34.8051948051948, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 46130; Mature: 46130

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHTPNAESPIAMASPLPRRSRQARIAWGDRVVTVGGDAPVRVQSMTNTDTVDAIATAIQ
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCEEEEECCCCHHHHHHHHHHH
VKELAQAGSEFVRITVNTPEAAAAVPYIREQLDRMGETVPLVGDFHYNGHRLLTEYPDCA
HHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCHHH
QALSKYRINPGNVGKGDKRDRQFGQMIEAAMRWNKAVRIGVNWGSLDQELLAGLMDANSR
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCC
RAVPWDARQVMYEALITSAIESAQRAEAMGLDGNQIILSCKVSGVQDLIAVYRELARRCD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCC
YALHLGLTEAGMGTKGTVASAAALSVLLQEGIGDTIRVSLTPQPGEARTQEVIVASEILQ
CEEEECCHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
ALGLRVFVPSVTACPGCGRTTSTTFQELAKQIDDFLRSQMPVWRTRYPGVEKMKVAVMGC
HCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHEEEEEE
IVNGPGESKHADIGISLPGTGEAPAAPVFIDGEKAMTLRGEHIARDFQQVVEDYISRRFG
EECCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECHHHHHHHHHHHHHHHHHHHCC
STAEVL
CCCCCC
>Mature Secondary Structure
MIHTPNAESPIAMASPLPRRSRQARIAWGDRVVTVGGDAPVRVQSMTNTDTVDAIATAIQ
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCEEEEECCCCHHHHHHHHHHH
VKELAQAGSEFVRITVNTPEAAAAVPYIREQLDRMGETVPLVGDFHYNGHRLLTEYPDCA
HHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCHHH
QALSKYRINPGNVGKGDKRDRQFGQMIEAAMRWNKAVRIGVNWGSLDQELLAGLMDANSR
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCC
RAVPWDARQVMYEALITSAIESAQRAEAMGLDGNQIILSCKVSGVQDLIAVYRELARRCD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCC
YALHLGLTEAGMGTKGTVASAAALSVLLQEGIGDTIRVSLTPQPGEARTQEVIVASEILQ
CEEEECCHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
ALGLRVFVPSVTACPGCGRTTSTTFQELAKQIDDFLRSQMPVWRTRYPGVEKMKVAVMGC
HCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHEEEEEE
IVNGPGESKHADIGISLPGTGEAPAAPVFIDGEKAMTLRGEHIARDFQQVVEDYISRRFG
EECCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECHHHHHHHHHHHHHHHHHHHCC
STAEVL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]