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The map label for this gene is rlmN

Identifier: 120610108

GI number: 120610108

Start: 1561396

End: 1562517

Strand: Direct

Name: rlmN

Synonym: Aave_1421

Alternate gene names: 120610108

Gene position: 1561396-1562517 (Clockwise)

Preceding gene: 120610107

Following gene: 120610109

Centisome position: 29.17

GC content: 66.67

Gene sequence:

>1122_bases
ATGACCAAGAACCTTCTCGACTTCGACCTGGACGGGCTCGCCGCCTTCTGCGAGCAGCTCGGGGAGAAGCGTTTCCGCGC
GGTCCAGCTTTTCCGCTGGATCCACCAGCGCGGCGCCAGCGATTTCGCCCGGATGAGCGACCTGGCGAAGTCGCTGCGCG
AGAAGCTCTCGGGCTGCGCCCATGTCGCGGCCCTGCCGGTCATCAGCGAGCACGTGTCCGCCGATGGCACCGTCAAGTGG
CTCTTCGACGTCGGCGACGGCAATGCGGTCGAGTCCGTGTTCATCCCCGAGGATGACCGCGGCACGCTGTGCATTTCCTC
GCAGGCCGGCTGCGCGGTGGGCTGCCGCTTCTGCTCCACGGGGCACCAGGGTTTCAGCCGCAACCTCACCAGCGGCGAGA
TCGTCGCCCAGCTGTGGTTCGCCGAGCACGCCCTGCGCGCACGCCTGGGAACCCAGGAGCGTGTCATCTCCAACGTGGTC
ATGATGGGCATGGGCGAACCCTTGCAGAACTACACCGCACTGGTGCCGGCGCTGCGCACCATGCTCGACGACCATGGCTA
TGGCCTGTCGCGGCGGCGCCTCACGGTATCCACCTCCGGTGTCGTGCCCATGATGGACCGCCTGTCACAGGACTGCGCCG
TGGCGATGGCCGTGTCGCTGCATGCGCCGAACGACGCCCTGCGGGACCAGCTGGTTCCCCTGAACCGCAAATACCCCCTG
CGCGAACTGCTCGACGCCTGCACGCGTTACCTGGAGCATGCGCCGCGGGATTTCATCACCTTCGAATACTGCATGCTCGA
TGGCGTCAACGACCAGCCCGAGCATGCGCGCCAGCTCATCGACCTCGTGCGCCCGCGCGGCGGGGAGGGCGTGCGCTGCA
AGTTCAACCTGATTCCCTTCAACCCGTTTCCCGCTTCCGGACTGCACCGTTCCAACCCTCAACAGGTGGCGGCTTTCGCG
AAGATGCTCAGCGATGCGGGTATCGTTACGACCGTGCGCAAGACACGCGGCGATGACATCGACGCGGCCTGCGGGCAGCT
CGCGGGCGATGTGAAGGACCGCACCCGGGCGGCCGAACGCATGGCCAGGCAGCGCACGATCGTTCTCAAACCGGTGGCCT
GA

Upstream 100 bases:

>100_bases
TTCCCCGGCCTGAACATCTACGCACGCTGATCCCGCTGGCGGCCCGGCGCGGCGCCTGCGCCATGCCCCGGGCCCTGCCA
GCCGGAACGGCTCCCCGACG

Downstream 100 bases:

>100_bases
AGGCCGCCCCGTTCTGGAGGCTTGAGATGGTGGAACCCCGATCGTTCCTGCGGCATGCGTCCCGCCACGTCCTCGCGGCC
GGCGCAGTCGTGTGCCTGGC

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTKNLLDFDLDGLAAFCEQLGEKRFRAVQLFRWIHQRGASDFARMSDLAKSLREKLSGCAHVAALPVISEHVSADGTVKW
LFDVGDGNAVESVFIPEDDRGTLCISSQAGCAVGCRFCSTGHQGFSRNLTSGEIVAQLWFAEHALRARLGTQERVISNVV
MMGMGEPLQNYTALVPALRTMLDDHGYGLSRRRLTVSTSGVVPMMDRLSQDCAVAMAVSLHAPNDALRDQLVPLNRKYPL
RELLDACTRYLEHAPRDFITFEYCMLDGVNDQPEHARQLIDLVRPRGGEGVRCKFNLIPFNPFPASGLHRSNPQQVAAFA
KMLSDAGIVTTVRKTRGDDIDAACGQLAGDVKDRTRAAERMARQRTIVLKPVA

Sequences:

>Translated_373_residues
MTKNLLDFDLDGLAAFCEQLGEKRFRAVQLFRWIHQRGASDFARMSDLAKSLREKLSGCAHVAALPVISEHVSADGTVKW
LFDVGDGNAVESVFIPEDDRGTLCISSQAGCAVGCRFCSTGHQGFSRNLTSGEIVAQLWFAEHALRARLGTQERVISNVV
MMGMGEPLQNYTALVPALRTMLDDHGYGLSRRRLTVSTSGVVPMMDRLSQDCAVAMAVSLHAPNDALRDQLVPLNRKYPL
RELLDACTRYLEHAPRDFITFEYCMLDGVNDQPEHARQLIDLVRPRGGEGVRCKFNLIPFNPFPASGLHRSNPQQVAAFA
KMLSDAGIVTTVRKTRGDDIDAACGQLAGDVKDRTRAAERMARQRTIVLKPVA
>Mature_372_residues
TKNLLDFDLDGLAAFCEQLGEKRFRAVQLFRWIHQRGASDFARMSDLAKSLREKLSGCAHVAALPVISEHVSADGTVKWL
FDVGDGNAVESVFIPEDDRGTLCISSQAGCAVGCRFCSTGHQGFSRNLTSGEIVAQLWFAEHALRARLGTQERVISNVVM
MGMGEPLQNYTALVPALRTMLDDHGYGLSRRRLTVSTSGVVPMMDRLSQDCAVAMAVSLHAPNDALRDQLVPLNRKYPLR
ELLDACTRYLEHAPRDFITFEYCMLDGVNDQPEHARQLIDLVRPRGGEGVRCKFNLIPFNPFPASGLHRSNPQQVAAFAK
MLSDAGIVTTVRKTRGDDIDAACGQLAGDVKDRTRAAERMARQRTIVLKPVA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=364, Percent_Identity=52.1978021978022, Blast_Score=376, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_ACIAC (A1TM24)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969786.1
- ProteinModelPortal:   A1TM24
- STRING:   A1TM24
- GeneID:   4668433
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1421
- NMPDR:   fig|397945.5.peg.1246
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   A1TM24
- ProtClustDB:   CLSK2391655
- BioCyc:   AAVE397945:AAVE_1421-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41144; Mature: 41012

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNLLDFDLDGLAAFCEQLGEKRFRAVQLFRWIHQRGASDFARMSDLAKSLREKLSGCA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
HVAALPVISEHVSADGTVKWLFDVGDGNAVESVFIPEDDRGTLCISSQAGCAVGCRFCST
HHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHC
GHQGFSRNLTSGEIVAQLWFAEHALRARLGTQERVISNVVMMGMGEPLQNYTALVPALRT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MLDDHGYGLSRRRLTVSTSGVVPMMDRLSQDCAVAMAVSLHAPNDALRDQLVPLNRKYPL
HHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCH
RELLDACTRYLEHAPRDFITFEYCMLDGVNDQPEHARQLIDLVRPRGGEGVRCKFNLIPF
HHHHHHHHHHHHHCCCHHEEEHHHEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEC
NPFPASGLHRSNPQQVAAFAKMLSDAGIVTTVRKTRGDDIDAACGQLAGDVKDRTRAAER
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
MARQRTIVLKPVA
HHHHCEEEEECCC
>Mature Secondary Structure 
TKNLLDFDLDGLAAFCEQLGEKRFRAVQLFRWIHQRGASDFARMSDLAKSLREKLSGCA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
HVAALPVISEHVSADGTVKWLFDVGDGNAVESVFIPEDDRGTLCISSQAGCAVGCRFCST
HHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHC
GHQGFSRNLTSGEIVAQLWFAEHALRARLGTQERVISNVVMMGMGEPLQNYTALVPALRT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MLDDHGYGLSRRRLTVSTSGVVPMMDRLSQDCAVAMAVSLHAPNDALRDQLVPLNRKYPL
HHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCH
RELLDACTRYLEHAPRDFITFEYCMLDGVNDQPEHARQLIDLVRPRGGEGVRCKFNLIPF
HHHHHHHHHHHHHCCCHHEEEHHHEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEC
NPFPASGLHRSNPQQVAAFAKMLSDAGIVTTVRKTRGDDIDAACGQLAGDVKDRTRAAER
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
MARQRTIVLKPVA
HHHHCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA