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The map label for this gene is pyrG

Identifier: 120610014

GI number: 120610014

Start: 1452597

End: 1454276

Strand: Reverse

Name: pyrG

Synonym: Aave_1327

Alternate gene names: 120610014

Gene position: 1454276-1452597 (Counterclockwise)

Preceding gene: 120610018

Following gene: 120610013

Centisome position: 27.17

GC content: 66.07

Gene sequence:

>1680_bases
ATGACCAAATTTGTCTTCGTCACCGGCGGTGTGGTGTCTTCCCTGGGCAAGGGAATCGCCTCAGCCTCCCTTGCTGCGAT
CCTCGAATCGCGCGGCCTCAAAGTCACCCTCATCAAGCTCGACCCGTACATCAACGTGGACCCGGGCACCATGTCCCCGT
TCCAGCACGGCGAAGTGTTCGTCACCGACGACGGCGCCGAGACCGACCTCGACCTCGGCCACTATGAGCGTTTCATCGAA
ACGCGCATGAAGAAGACCAACAACTTCACCACGGGCCGCATCTACCAGTCCGTGCTGGAGAAGGAGCGCCGCGGCGACTA
CCTGGGCAAGACGGTGCAGGTGATCCCGCACGTCACCAACGAGATCCAGGAATACGTCAAGCGCGGCGCGGGCATCGGCA
CCGGCGACGCGGTGGACGTGGCCATCGTCGAGATCGGCGGCACGGTGGGCGATATCGAATCCCTGCCCTTCCTCGAAGCC
GTGCGCCAGATGAGCCTGCGCATGGGGCCGAACAATTCCGCGTTCGTTCACCTCACCTACCTGCCCTACATCGCGGCGGC
CGGTGAACTCAAGACCAAGCCCACCCAGCACACGGTGCAGAAGCTGCGCGAGATCGGCATCCAGCCCGACGCCCTGCTCT
GCCGTGCAGACCGCCGCATCCCCGACGAGGAGCGCGGCAAGATCTCCCTGTTCACCAACGTGGCCGAGTGGGGCGTGATC
AGCATGTGGGACGTGGACACCATCTACAAGGTGCCCCGCATGCTGCACGAGCAGGGCCTGGACGGCCTGATCTGCGACAA
GCTGCGCCTGAACACGCCGCCCACCAGCCTCAAGCGCTGGGACCAGCTCGTGTACGAGACCGAACATCCGCAGGGCGAGG
TCACCATCGCCATGGTGGGCAAGTACGTCGATCTGTCCGACAGCTACAAGTCGGTGAACGAGGCACTGCGCCACGCCGGC
ATGAAGAACCACGTGCGCGTGAAGATCGAGCACGTCGATTCCGAGACCATCGCACCGGGCGATGCGGCCGAGAAGCTCGC
CAAGTACGACGCCATCCTGGTGCCCGGCGGCTTCGGCGCGCGCGGCGTGGAAGGCAAGATCTGCACGGCGCGCTACGCCC
GCGAGCAGAGGGTGCCCTACCTCGGCATCTGCCTGGGCATGCAGGTGGCCACCATCGAATACGCGCGCCACGTGGCGGGC
CTGGCGAACGCCAACAGCACCGAGTTCGACGCCGCCACGCCGCACCCCGTGATCGCGCTGATCACCGAATGGAAGGACGC
CGACGGCACCATCCAGACGCGCAGCGAAACCTCCGACCTGGGCGGCACCATGCGCCTGGGCGCGCAGAGCTCCGACGTCG
CCTCCGGCACGCTGGCGCACGAGATCTACGGCAACATCGTCACCGAGCGCCACCGCCACCGGTACGAGGCGAACGTGAAC
TACCTCGACCGGCTCCGCAAGGCGGGCCTCGTGATCTCCGCGCTCACGCAGCGCGAGCAGCTCACCGAGATCGTCGAGCT
GCCGCGCGAGGTGCATCCGTGGTTCATCGGCGTGCAGTTCCACCCCGAGTTCAAGTCCACCCCGTGGGACGGCCATCCGC
TCTTCAACGCCTTCATCAAGGCGGCGATCGACCACCAGAAGAGCCGCCAGCCCACCCTGCCCGCCGCCGCTGCCTCCTGA

Upstream 100 bases:

>100_bases
TTAGCAGACGCCCTGCCACGGTGCAGGGGGAAGGCGCCGGTCGGCCGGGCGCGGATGGGCCGGCCTCTATAATCCCGATT
TCCCACCACCATAAAAAGAC

Downstream 100 bases:

>100_bases
GGCGTCGGCGCTTTCCCCCCAAGGCACCTTCCATGAAACTCTGCGGCTTCGACGTCGGCCTCGACCGGCCCTTCTTCCTC
ATCGCGGGTCCCTGCGTCAT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MTKFVFVTGGVVSSLGKGIASASLAAILESRGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIE
TRMKKTNNFTTGRIYQSVLEKERRGDYLGKTVQVIPHVTNEIQEYVKRGAGIGTGDAVDVAIVEIGGTVGDIESLPFLEA
VRQMSLRMGPNNSAFVHLTYLPYIAAAGELKTKPTQHTVQKLREIGIQPDALLCRADRRIPDEERGKISLFTNVAEWGVI
SMWDVDTIYKVPRMLHEQGLDGLICDKLRLNTPPTSLKRWDQLVYETEHPQGEVTIAMVGKYVDLSDSYKSVNEALRHAG
MKNHVRVKIEHVDSETIAPGDAAEKLAKYDAILVPGGFGARGVEGKICTARYAREQRVPYLGICLGMQVATIEYARHVAG
LANANSTEFDAATPHPVIALITEWKDADGTIQTRSETSDLGGTMRLGAQSSDVASGTLAHEIYGNIVTERHRHRYEANVN
YLDRLRKAGLVISALTQREQLTEIVELPREVHPWFIGVQFHPEFKSTPWDGHPLFNAFIKAAIDHQKSRQPTLPAAAAS

Sequences:

>Translated_559_residues
MTKFVFVTGGVVSSLGKGIASASLAAILESRGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIE
TRMKKTNNFTTGRIYQSVLEKERRGDYLGKTVQVIPHVTNEIQEYVKRGAGIGTGDAVDVAIVEIGGTVGDIESLPFLEA
VRQMSLRMGPNNSAFVHLTYLPYIAAAGELKTKPTQHTVQKLREIGIQPDALLCRADRRIPDEERGKISLFTNVAEWGVI
SMWDVDTIYKVPRMLHEQGLDGLICDKLRLNTPPTSLKRWDQLVYETEHPQGEVTIAMVGKYVDLSDSYKSVNEALRHAG
MKNHVRVKIEHVDSETIAPGDAAEKLAKYDAILVPGGFGARGVEGKICTARYAREQRVPYLGICLGMQVATIEYARHVAG
LANANSTEFDAATPHPVIALITEWKDADGTIQTRSETSDLGGTMRLGAQSSDVASGTLAHEIYGNIVTERHRHRYEANVN
YLDRLRKAGLVISALTQREQLTEIVELPREVHPWFIGVQFHPEFKSTPWDGHPLFNAFIKAAIDHQKSRQPTLPAAAAS
>Mature_558_residues
TKFVFVTGGVVSSLGKGIASASLAAILESRGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIET
RMKKTNNFTTGRIYQSVLEKERRGDYLGKTVQVIPHVTNEIQEYVKRGAGIGTGDAVDVAIVEIGGTVGDIESLPFLEAV
RQMSLRMGPNNSAFVHLTYLPYIAAAGELKTKPTQHTVQKLREIGIQPDALLCRADRRIPDEERGKISLFTNVAEWGVIS
MWDVDTIYKVPRMLHEQGLDGLICDKLRLNTPPTSLKRWDQLVYETEHPQGEVTIAMVGKYVDLSDSYKSVNEALRHAGM
KNHVRVKIEHVDSETIAPGDAAEKLAKYDAILVPGGFGARGVEGKICTARYAREQRVPYLGICLGMQVATIEYARHVAGL
ANANSTEFDAATPHPVIALITEWKDADGTIQTRSETSDLGGTMRLGAQSSDVASGTLAHEIYGNIVTERHRHRYEANVNY
LDRLRKAGLVISALTQREQLTEIVELPREVHPWFIGVQFHPEFKSTPWDGHPLFNAFIKAAIDHQKSRQPTLPAAAAS

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=560, Percent_Identity=43.9285714285714, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=559, Percent_Identity=42.397137745975, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI28559083, Length=559, Percent_Identity=42.397137745975, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI221316689, Length=559, Percent_Identity=42.397137745975, Blast_Score=459, Evalue=1e-129,
Organism=Escherichia coli, GI1789142, Length=549, Percent_Identity=62.1129326047359, Blast_Score=692, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=623, Percent_Identity=39.1653290529695, Blast_Score=429, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322563, Length=572, Percent_Identity=41.4335664335664, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6319432, Length=570, Percent_Identity=40.7017543859649, Blast_Score=432, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24664469, Length=561, Percent_Identity=43.49376114082, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI21357815, Length=506, Percent_Identity=42.0948616600791, Blast_Score=388, Evalue=1e-108,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_ACIAC (A1TLT0)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969692.1
- ProteinModelPortal:   A1TLT0
- STRING:   A1TLT0
- GeneID:   4666167
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1327
- NMPDR:   fig|397945.5.peg.1168
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- PhylomeDB:   A1TLT0
- ProtClustDB:   PRK05380
- BioCyc:   AAVE397945:AAVE_1327-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 61718; Mature: 61587

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 384-384 ACT_SITE 521-521 ACT_SITE 523-523

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFVFVTGGVVSSLGKGIASASLAAILESRGLKVTLIKLDPYINVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFIETRMKKTNNFTTGRIYQSVLEKERRGDYLGKTVQVIPHVTN
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHCCHHHHHHHHHH
EIQEYVKRGAGIGTGDAVDVAIVEIGGTVGDIESLPFLEAVRQMSLRMGPNNSAFVHLTY
HHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
LPYIAAAGELKTKPTQHTVQKLREIGIQPDALLCRADRRIPDEERGKISLFTNVAEWGVI
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEEEEEHHHCCEE
SMWDVDTIYKVPRMLHEQGLDGLICDKLRLNTPPTSLKRWDQLVYETEHPQGEVTIAMVG
EEECHHHHHHHHHHHHHCCCCCEEECCEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEEC
KYVDLSDSYKSVNEALRHAGMKNHVRVKIEHVDSETIAPGDAAEKLAKYDAILVPGGFGA
CEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCEEEECCCCCC
RGVEGKICTARYAREQRVPYLGICLGMQVATIEYARHVAGLANANSTEFDAATPHPVIAL
CCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
ITEWKDADGTIQTRSETSDLGGTMRLGAQSSDVASGTLAHEIYGNIVTERHRHRYEANVN
EEECCCCCCCEEECCCHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
YLDRLRKAGLVISALTQREQLTEIVELPREVHPWFIGVQFHPEFKSTPWDGHPLFNAFIK
HHHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
AAIDHQKSRQPTLPAAAAS
HHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TKFVFVTGGVVSSLGKGIASASLAAILESRGLKVTLIKLDPYINVDPGTMSPFQHGEVF
CEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFIETRMKKTNNFTTGRIYQSVLEKERRGDYLGKTVQVIPHVTN
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHCCHHHHHHHHHH
EIQEYVKRGAGIGTGDAVDVAIVEIGGTVGDIESLPFLEAVRQMSLRMGPNNSAFVHLTY
HHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
LPYIAAAGELKTKPTQHTVQKLREIGIQPDALLCRADRRIPDEERGKISLFTNVAEWGVI
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEEEEEHHHCCEE
SMWDVDTIYKVPRMLHEQGLDGLICDKLRLNTPPTSLKRWDQLVYETEHPQGEVTIAMVG
EEECHHHHHHHHHHHHHCCCCCEEECCEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEEC
KYVDLSDSYKSVNEALRHAGMKNHVRVKIEHVDSETIAPGDAAEKLAKYDAILVPGGFGA
CEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCEEEECCCCCC
RGVEGKICTARYAREQRVPYLGICLGMQVATIEYARHVAGLANANSTEFDAATPHPVIAL
CCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
ITEWKDADGTIQTRSETSDLGGTMRLGAQSSDVASGTLAHEIYGNIVTERHRHRYEANVN
EEECCCCCCCEEECCCHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
YLDRLRKAGLVISALTQREQLTEIVELPREVHPWFIGVQFHPEFKSTPWDGHPLFNAFIK
HHHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
AAIDHQKSRQPTLPAAAAS
HHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA