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The map label for this gene is pyrB [H]

Identifier: 120609997

GI number: 120609997

Start: 1437163

End: 1438224

Strand: Direct

Name: pyrB [H]

Synonym: Aave_1310

Alternate gene names: 120609997

Gene position: 1437163-1438224 (Clockwise)

Preceding gene: 120609994

Following gene: 120609998

Centisome position: 26.85

GC content: 70.43

Gene sequence:

>1062_bases
GTGTCGCAGTGCCATCGCCCCCAGCCCGCCGACGGGCCCACACGCATCCCACCCCACCGGGCCGGGCACAAGAACCTCGT
GTCGATCGATGACTGGTCGCGGGGGGATATCGAGAACCTGCTGGACGTGACCGAGGACATCTGCCGCCATCCGGGCGAGT
TCGCCTACAGCCTCGCCGGCTCGCTGGTCTGCACCGCGTTCTTCGAGCCCAGCACCCGTACGCGCCTGAGCTTCGAGGCG
GCTGCGCACCGGCTGGGCGCGAAGGTGCTGAGCATGGGCGACATGCAGACCACCCGCATGGGCCTGGGGGAAAGCGCCTC
CGACACCCTGCGCATGGCGGGCTACTATGCGGACCTGGTCGTGGCGCGGCATGCGCAGGACGGCATGGTGGAGCGCATGG
CGCAGGCGCTGGACGTGCCCGTGGTGAACGCGGGCGAAGGCGTGCACCGCCATCCGACGCAGACCCTGATCGACCTCTTC
ACCATGCGCAAGCACTTCGGCACGCTGGACGGCCTGCGCGTGGGCATCTGCGGCGGGTTGCGCTATTCGCGGGCGGCCAA
GTCGCTGCTGGCCGGGCTGCGTGCCTTCCGCGACGTGCGGGTGCACGTCGTGGATGCAGTGGGAGACGCCGATGAGGCCC
AGCCCGTGCCGCTGGCGCAGGTGGTCGGCGCCGGCGCCCAGGAGCATGCATGCGTCGCCGACATGCTCGGCCAGGTGGAT
GTGCTCTACGTCGTGCGCGTGCAGCGCGAGCAGTTCCGCGACGCGGCCTCCCATGGGGCGCAGCTGGAGCGCTGCCGCGT
GGATCGCCGTCTGCTGCAGTGCCTGCGGGGCGGCATGGTGGTGATGCATTGCCTCCCCCGTGGCGGCGAGCTCCCGGAAG
ACGTGGACGACACCCCCTTCAACCACTATTTCCGGCAGGCCGCCAACGGCGTGGCCGTGCGCATGGCGGTGCTCCAGCGC
TACCTGGGGCGGTTCCGCCTGACCACGGCTGCCCTGATGGGCGACAGCCTGCACCTGGACATCGCGCCTCCGTCTTCCGC
CATCAGGTCCGTCGCATGGTGA

Upstream 100 bases:

>100_bases
TTTTATGTTTCGTATAAGAAAGAAAAACTTCTCTTATGGAAGCCTCGGAAAGAAGATTGAAGCAGAAAGTCAGTCCATCC
GCACAACAAGATTGTTTCCA

Downstream 100 bases:

>100_bases
TGCTCTTGAGCGCTGCCATCGCGGGCGTGGGAGGCATTGTCGGCATCGTCGCCGTCACCGTGGGCGGAGGCGTCACCCTC
GGCGTGCCGCTGCTGCTGCT

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MSQCHRPQPADGPTRIPPHRAGHKNLVSIDDWSRGDIENLLDVTEDICRHPGEFAYSLAGSLVCTAFFEPSTRTRLSFEA
AAHRLGAKVLSMGDMQTTRMGLGESASDTLRMAGYYADLVVARHAQDGMVERMAQALDVPVVNAGEGVHRHPTQTLIDLF
TMRKHFGTLDGLRVGICGGLRYSRAAKSLLAGLRAFRDVRVHVVDAVGDADEAQPVPLAQVVGAGAQEHACVADMLGQVD
VLYVVRVQREQFRDAASHGAQLERCRVDRRLLQCLRGGMVVMHCLPRGGELPEDVDDTPFNHYFRQAANGVAVRMAVLQR
YLGRFRLTTAALMGDSLHLDIAPPSSAIRSVAW

Sequences:

>Translated_353_residues
MSQCHRPQPADGPTRIPPHRAGHKNLVSIDDWSRGDIENLLDVTEDICRHPGEFAYSLAGSLVCTAFFEPSTRTRLSFEA
AAHRLGAKVLSMGDMQTTRMGLGESASDTLRMAGYYADLVVARHAQDGMVERMAQALDVPVVNAGEGVHRHPTQTLIDLF
TMRKHFGTLDGLRVGICGGLRYSRAAKSLLAGLRAFRDVRVHVVDAVGDADEAQPVPLAQVVGAGAQEHACVADMLGQVD
VLYVVRVQREQFRDAASHGAQLERCRVDRRLLQCLRGGMVVMHCLPRGGELPEDVDDTPFNHYFRQAANGVAVRMAVLQR
YLGRFRLTTAALMGDSLHLDIAPPSSAIRSVAW
>Mature_352_residues
SQCHRPQPADGPTRIPPHRAGHKNLVSIDDWSRGDIENLLDVTEDICRHPGEFAYSLAGSLVCTAFFEPSTRTRLSFEAA
AHRLGAKVLSMGDMQTTRMGLGESASDTLRMAGYYADLVVARHAQDGMVERMAQALDVPVVNAGEGVHRHPTQTLIDLFT
MRKHFGTLDGLRVGICGGLRYSRAAKSLLAGLRAFRDVRVHVVDAVGDADEAQPVPLAQVVGAGAQEHACVADMLGQVDV
LYVVRVQREQFRDAASHGAQLERCRVDRRLLQCLRGGMVVMHCLPRGGELPEDVDDTPFNHYFRQAANGVAVRMAVLQRY
LGRFRLTTAALMGDSLHLDIAPPSSAIRSVAW

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=270, Percent_Identity=38.1481481481481, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI38788445, Length=316, Percent_Identity=25.9493670886076, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI2367364, Length=305, Percent_Identity=36.3934426229508, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1790703, Length=275, Percent_Identity=29.0909090909091, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786469, Length=146, Percent_Identity=35.6164383561644, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=348, Percent_Identity=31.8965517241379, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322331, Length=315, Percent_Identity=33.015873015873, Blast_Score=161, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24642586, Length=268, Percent_Identity=35.8208955223881, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 38610; Mature: 38478

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQCHRPQPADGPTRIPPHRAGHKNLVSIDDWSRGDIENLLDVTEDICRHPGEFAYSLAG
CCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
SLVCTAFFEPSTRTRLSFEAAAHRLGAKVLSMGDMQTTRMGLGESASDTLRMAGYYADLV
HHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHH
VARHAQDGMVERMAQALDVPVVNAGEGVHRHPTQTLIDLFTMRKHFGTLDGLRVGICGGL
HHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
RYSRAAKSLLAGLRAFRDVRVHVVDAVGDADEAQPVPLAQVVGAGAQEHACVADMLGQVD
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCHH
VLYVVRVQREQFRDAASHGAQLERCRVDRRLLQCLRGGMVVMHCLPRGGELPEDVDDTPF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCH
NHYFRQAANGVAVRMAVLQRYLGRFRLTTAALMGDSLHLDIAPPSSAIRSVAW
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCC
>Mature Secondary Structure 
SQCHRPQPADGPTRIPPHRAGHKNLVSIDDWSRGDIENLLDVTEDICRHPGEFAYSLAG
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
SLVCTAFFEPSTRTRLSFEAAAHRLGAKVLSMGDMQTTRMGLGESASDTLRMAGYYADLV
HHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHH
VARHAQDGMVERMAQALDVPVVNAGEGVHRHPTQTLIDLFTMRKHFGTLDGLRVGICGGL
HHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
RYSRAAKSLLAGLRAFRDVRVHVVDAVGDADEAQPVPLAQVVGAGAQEHACVADMLGQVD
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCHH
VLYVVRVQREQFRDAASHGAQLERCRVDRRLLQCLRGGMVVMHCLPRGGELPEDVDDTPF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCH
NHYFRQAANGVAVRMAVLQRYLGRFRLTTAALMGDSLHLDIAPPSSAIRSVAW
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA