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The map label for this gene is nuoI

Identifier: 120609958

GI number: 120609958

Start: 1395839

End: 1396354

Strand: Direct

Name: nuoI

Synonym: Aave_1271

Alternate gene names: 120609958

Gene position: 1395839-1396354 (Clockwise)

Preceding gene: 120609957

Following gene: 120609959

Centisome position: 26.08

GC content: 64.34

Gene sequence:

>516_bases
ATGGCTGCTGTTGCTGCTGCTACGACATCTTTTTCCTTCAAGGACTTTTTCAAGAGCTTCATGCTCGTGGAACTGGTCAA
GGGCATGGCCCTGACCGGCCGCTACGCGTTCCGCCGCAAGGTGACGGTGCAGTTCCCCGAAGAGAAGACTCCGCTGTCGC
CGCGCTTCCGCGGGCTGCACGCGCTGCGCCGCTACGAAAACGGCGAAGAGCGCTGCATCGCCTGCAAGCTCTGCGAGGCG
GTCTGCCCGGCGCTCGCGATCACGATCGAGTCGGACGTGCGTGCCGACGGCTCGCGCCGCACGACGCGCTACGACATCGA
CCTCACGAAGTGCATCTTCTGCGGCTTCTGCGAGGAGAGCTGCCCGGTCGATTCGATCGTCGAGACGCAGATCCTGGAAT
ACCACGGCGAGAAGCGGGGCGACCTGTACTTCACCAAGGACATGCTCCTGGCGGTCGGCGACCGCTACGAGGCCGAGATC
GCCGCTGCCAAGGCGGCCGACGCCAAGTACCGCTGA

Upstream 100 bases:

>100_bases
CTGGAAGATCTTCATTCCGGTCACCCTGGTGTGGCTGCTGATCGTGGGCGGATGGCTGCTGTCGCCCTGGAACATCTGGA
AATAAGCGGGACACGACATC

Downstream 100 bases:

>100_bases
CCAGCGGCTCCTGAACCACTTTTCACAAGATCCGATTCATGGACGCCAAGACTGGTTTCTTCTATCTGTTCTCGGTCGTG
CTGCTGTATGCGGCCTTCCG

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MAAVAAATTSFSFKDFFKSFMLVELVKGMALTGRYAFRRKVTVQFPEEKTPLSPRFRGLHALRRYENGEERCIACKLCEA
VCPALAITIESDVRADGSRRTTRYDIDLTKCIFCGFCEESCPVDSIVETQILEYHGEKRGDLYFTKDMLLAVGDRYEAEI
AAAKAADAKYR

Sequences:

>Translated_171_residues
MAAVAAATTSFSFKDFFKSFMLVELVKGMALTGRYAFRRKVTVQFPEEKTPLSPRFRGLHALRRYENGEERCIACKLCEA
VCPALAITIESDVRADGSRRTTRYDIDLTKCIFCGFCEESCPVDSIVETQILEYHGEKRGDLYFTKDMLLAVGDRYEAEI
AAAKAADAKYR
>Mature_170_residues
AAVAAATTSFSFKDFFKSFMLVELVKGMALTGRYAFRRKVTVQFPEEKTPLSPRFRGLHALRRYENGEERCIACKLCEAV
CPALAITIESDVRADGSRRTTRYDIDLTKCIFCGFCEESCPVDSIVETQILEYHGEKRGDLYFTKDMLLAVGDRYEAEIA
AAKAADAKYR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=154, Percent_Identity=61.038961038961, Blast_Score=204, Evalue=4e-53,
Organism=Escherichia coli, GI1788617, Length=128, Percent_Identity=39.84375, Blast_Score=102, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17555194, Length=155, Percent_Identity=55.4838709677419, Blast_Score=186, Evalue=7e-48,
Organism=Drosophila melanogaster, GI17864306, Length=148, Percent_Identity=61.4864864864865, Blast_Score=196, Evalue=4e-51,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_ACIAC (A1TLM4)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969636.1
- ProteinModelPortal:   A1TLM4
- SMR:   A1TLM4
- STRING:   A1TLM4
- GeneID:   4667686
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1271
- NMPDR:   fig|397945.5.peg.1115
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   NYPYEKG
- PhylomeDB:   A1TLM4
- ProtClustDB:   PRK05888
- BioCyc:   AAVE397945:AAVE_1271-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 19282; Mature: 19151

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVAAATTSFSFKDFFKSFMLVELVKGMALTGRYAFRRKVTVQFPEEKTPLSPRFRGLH
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCCHHHHHHH
ALRRYENGEERCIACKLCEAVCPALAITIESDVRADGSRRTTRYDIDLTKCIFCGFCEES
HHHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEECCHHHHHHHHCCCCC
CPVDSIVETQILEYHGEKRGDLYFTKDMLLAVGDRYEAEIAAAKAADAKYR
CCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AAVAAATTSFSFKDFFKSFMLVELVKGMALTGRYAFRRKVTVQFPEEKTPLSPRFRGLH
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCCHHHHHHH
ALRRYENGEERCIACKLCEAVCPALAITIESDVRADGSRRTTRYDIDLTKCIFCGFCEES
HHHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEECCHHHHHHHHCCCCC
CPVDSIVETQILEYHGEKRGDLYFTKDMLLAVGDRYEAEIAAAKAADAKYR
CCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA