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The map label for this gene is nuoB

Identifier: 120609951

GI number: 120609951

Start: 1388320

End: 1388799

Strand: Direct

Name: nuoB

Synonym: Aave_1264

Alternate gene names: 120609951

Gene position: 1388320-1388799 (Clockwise)

Preceding gene: 120609950

Following gene: 120609952

Centisome position: 25.94

GC content: 66.25

Gene sequence:

>480_bases
ATGATTGAAGGCGTGATGAAGGAAGGCTTCATCACCACGAGCTATGACTCCGTGGTGAACTGGGCCAAGACCGGATCGCT
GTGGCCCATGACCTTCGGTCTGGCCTGCTGCGCCGTCGAGATGATGCATGCCGCCGCGGCGCGCTACGACATCGGCCGCT
TCGGCGCCGAGGTGTTCCGCGCCAGCCCCCGGCAGTCCGATCTGATGATCGTGGCCGGCACGCTCTGCAACAAGATGGCC
CCGGCCCTGCGCAAGGTGTACGACCAGATGTCGGAGCCGCGCTGGGTGATCTCCATGGGCTCCTGCGCCAACGGCGGCGG
CTACTACCACTACAGCTACTCGGTGGTGCGCGGCTGCGACCGCATCGTGCCGGTGGACGTGTACGTGCCGGGCTGCCCCC
CGACCGCCGAGGCGCTCATCTACGGGATCATCCAGCTCCAGCAGAAGATCCGCCGCACCCATACCATCGCCCGCGTCTGA

Upstream 100 bases:

>100_bases
CGTCATCGTCTTCCTGGCCATCCTGGTCGTGGGCTTCGCCTACGAGTGGAAAAAGGGTGCCCTGGATTGGGAATGACGGG
GCTTCACAAGGAACGACACG

Downstream 100 bases:

>100_bases
AGGGTTCTCCAGCATGACAGCCATTGCCATCCGACCCGAGGCTCTACGGGACGCGGTCGCCGCTGCGCTCGGCGACAAGG
TGCGCGACATCGTGCTCGCG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MIEGVMKEGFITTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAAAARYDIGRFGAEVFRASPRQSDLMIVAGTLCNKMA
PALRKVYDQMSEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGIIQLQQKIRRTHTIARV

Sequences:

>Translated_159_residues
MIEGVMKEGFITTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAAAARYDIGRFGAEVFRASPRQSDLMIVAGTLCNKMA
PALRKVYDQMSEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGIIQLQQKIRRTHTIARV
>Mature_159_residues
MIEGVMKEGFITTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAAAARYDIGRFGAEVFRASPRQSDLMIVAGTLCNKMA
PALRKVYDQMSEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGIIQLQQKIRRTHTIARV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=78.2312925170068, Blast_Score=253, Evalue=6e-68,
Organism=Escherichia coli, GI1788624, Length=149, Percent_Identity=53.0201342281879, Blast_Score=178, Evalue=1e-46,
Organism=Escherichia coli, GI1789074, Length=123, Percent_Identity=41.4634146341463, Blast_Score=93, Evalue=7e-21,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=41.4634146341463, Blast_Score=90, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=148, Percent_Identity=70.2702702702703, Blast_Score=236, Evalue=5e-63,
Organism=Drosophila melanogaster, GI18859983, Length=141, Percent_Identity=78.0141843971631, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24642371, Length=141, Percent_Identity=78.0141843971631, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24651058, Length=141, Percent_Identity=78.7234042553192, Blast_Score=246, Evalue=3e-66,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_ACIAC (A1TLL7)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969629.1
- ProteinModelPortal:   A1TLL7
- SMR:   A1TLL7
- STRING:   A1TLL7
- GeneID:   4669001
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1264
- NMPDR:   fig|397945.5.peg.1108
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- PhylomeDB:   A1TLL7
- ProtClustDB:   PRK06411
- BioCyc:   AAVE397945:AAVE_1264-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 17569; Mature: 17569

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
9.4 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
9.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEGVMKEGFITTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAAAARYDIGRFGAEVFR
CCCCHHHCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASPRQSDLMIVAGTLCNKMAPALRKVYDQMSEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHCCC
RIVPVDVYVPGCPPTAEALIYGIIQLQQKIRRTHTIARV
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIEGVMKEGFITTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAAAARYDIGRFGAEVFR
CCCCHHHCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASPRQSDLMIVAGTLCNKMAPALRKVYDQMSEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHCCC
RIVPVDVYVPGCPPTAEALIYGIIQLQQKIRRTHTIARV
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA