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The map label for this gene is gsiB [H]

Identifier: 120609630

GI number: 120609630

Start: 1017943

End: 1019523

Strand: Direct

Name: gsiB [H]

Synonym: Aave_0936

Alternate gene names: 120609630

Gene position: 1017943-1019523 (Clockwise)

Preceding gene: 120609629

Following gene: 120609631

Centisome position: 19.02

GC content: 66.54

Gene sequence:

>1581_bases
ATGAATTTCCCACGCACGGCATTGCGCGCCGCCTTCGGCGCCTCCCTGATCGCGGCGGCCTGCATGGCGGGCGCGCAGAC
CATCCGGGTCGCCAACCAGGGCGACGCGCTGTCGATGGATCCGCATTCCCTGAACGAGAGCCTGCAGCTCAGCGTGACCG
GCAATATCTATGAGCCGCTCGTGGGCCGCAACAAGGACCTGAGCCTGGCGCCGGCGCTGGCCACGCGCTGGGCACAGCTC
TCTCCCACGGTGTGGCGCTTCGAACTGCGGCGTGGCGTGCTTTTCCACGACGGCACTCCGTTCACCGCGGACGACGTGCT
GTTTTCGCTGGCACGCACGCAGGCCGGGAGTTCGGACATGAAGACGTACACCAACGACTTCAAGGAAGTGCGCAAGGTGG
ACGACTACACCGTCGAGATCGAGACCAAAGCTCCGTACCCCATCCTGCCCGATGTGCTCACGCTCGTGTACATGATGTCG
CGCAAGTGGTGCGAGACGAACCACGCCACGGAGCCGGTGGATCGGCGCAGGGGCGTGGAGAACGCGGCGTCGTTCCGGGC
CAACGGCACGGGGCCGTTCCGGCTGCGCGAGCGCCAGCCCGGCGTGCGTACCGTGTTCACGCGCAACGCGCAGTACTGGG
GGGCCATCGAAGGCAACGTGTCCGAGGTGGTGTTCACCCCCATCGGCAACGACGCCACCCGCGTCGCGGCGCTGCTGTCG
GGCCAGGTGGACGTCATGGAGCCCGTGCCGGTGCAGGACATCGAGCGCGTGAACACGAGTCCGCTCACGCGCGCAGTGAC
CGGGCCCGAGCTGCGCACCATCTTCCTGGGCATGGACCAGAAGCGCGACGAGCTGCTGTATTCCAGCGTGAAGGGCAAAA
ACCCGTTCAAGGACAAGCGCGTGCGCCAGGCCTTCTACCAGGCCATCGACATCGAGGGCATCCGCAAGACGGTGATGCGC
GGCGCCTCCAATCCGTCGGCGCTGCTGGTGGGGCCGGGCGTGAACGGTTTCCAGGCGGATATCAAGCGCCTTCCCTACGA
CACGGATGCCGCGAAGAAGCTGCTGGCCGAAGCCGGCTACCCCAACGGCTTCGAGGTGGGCATGAACTGCCCCAACGACC
GCTACGTGAATGACGCGCGCATCTGCCAGGCAGTGGCCGCCAACCTCGCGCGCATCGGCGTGAAGGTGAACCTGCAGGCG
GAAACCAAGGGCACGTACTTCCCCAGGGTGCTGCGGCGGGATACCAGCTTCTACATGCTGGGCTGGATGCCCGCCACCTA
CGACGCGCACAACGCGATGACTGCGCTGATGGCCTGCGTGGACGACAAGGGCGCCGGGCAGTTCAACCTGGGCGGGTACT
GCAATCCCAAGGTGGACCAGCTCACGCGGCAGGTGCAGTCGGAGGTCGACAAGGCCCGGCGCGACGCGATGATCCGCGAA
GCCTTCGAGATCCATGCGGCCGACGTGGGCCACATCCCGCTGCACCAGCAGGCCCTGGCCTGGGGCGTGTCGCGCAAGGT
GGCGCTGGTGCAGATGGCCGACAACTTCATGCCGTTCAAGTGGATGAGCCTCGCGAAGTGA

Upstream 100 bases:

>100_bases
CCGGTACGACTTCAACGACGAGATCCTGCCCCTGGGCGCCGCGCTGCACGCGGGCCTGGTCGAGCAGGCCATGCCGCTGG
CAGCGGTCTGAGGTATGACG

Downstream 100 bases:

>100_bases
TGCCCTGATCCGTTCCGTCCGCGTGGAGATGCCGTTCCGCTCCACGCTTTTCCCGAATCGCACGATGAAAAAAACGCTTT
TCCGATGGTTCGACAGCGAT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MNFPRTALRAAFGASLIAAACMAGAQTIRVANQGDALSMDPHSLNESLQLSVTGNIYEPLVGRNKDLSLAPALATRWAQL
SPTVWRFELRRGVLFHDGTPFTADDVLFSLARTQAGSSDMKTYTNDFKEVRKVDDYTVEIETKAPYPILPDVLTLVYMMS
RKWCETNHATEPVDRRRGVENAASFRANGTGPFRLRERQPGVRTVFTRNAQYWGAIEGNVSEVVFTPIGNDATRVAALLS
GQVDVMEPVPVQDIERVNTSPLTRAVTGPELRTIFLGMDQKRDELLYSSVKGKNPFKDKRVRQAFYQAIDIEGIRKTVMR
GASNPSALLVGPGVNGFQADIKRLPYDTDAAKKLLAEAGYPNGFEVGMNCPNDRYVNDARICQAVAANLARIGVKVNLQA
ETKGTYFPRVLRRDTSFYMLGWMPATYDAHNAMTALMACVDDKGAGQFNLGGYCNPKVDQLTRQVQSEVDKARRDAMIRE
AFEIHAADVGHIPLHQQALAWGVSRKVALVQMADNFMPFKWMSLAK

Sequences:

>Translated_526_residues
MNFPRTALRAAFGASLIAAACMAGAQTIRVANQGDALSMDPHSLNESLQLSVTGNIYEPLVGRNKDLSLAPALATRWAQL
SPTVWRFELRRGVLFHDGTPFTADDVLFSLARTQAGSSDMKTYTNDFKEVRKVDDYTVEIETKAPYPILPDVLTLVYMMS
RKWCETNHATEPVDRRRGVENAASFRANGTGPFRLRERQPGVRTVFTRNAQYWGAIEGNVSEVVFTPIGNDATRVAALLS
GQVDVMEPVPVQDIERVNTSPLTRAVTGPELRTIFLGMDQKRDELLYSSVKGKNPFKDKRVRQAFYQAIDIEGIRKTVMR
GASNPSALLVGPGVNGFQADIKRLPYDTDAAKKLLAEAGYPNGFEVGMNCPNDRYVNDARICQAVAANLARIGVKVNLQA
ETKGTYFPRVLRRDTSFYMLGWMPATYDAHNAMTALMACVDDKGAGQFNLGGYCNPKVDQLTRQVQSEVDKARRDAMIRE
AFEIHAADVGHIPLHQQALAWGVSRKVALVQMADNFMPFKWMSLAK
>Mature_526_residues
MNFPRTALRAAFGASLIAAACMAGAQTIRVANQGDALSMDPHSLNESLQLSVTGNIYEPLVGRNKDLSLAPALATRWAQL
SPTVWRFELRRGVLFHDGTPFTADDVLFSLARTQAGSSDMKTYTNDFKEVRKVDDYTVEIETKAPYPILPDVLTLVYMMS
RKWCETNHATEPVDRRRGVENAASFRANGTGPFRLRERQPGVRTVFTRNAQYWGAIEGNVSEVVFTPIGNDATRVAALLS
GQVDVMEPVPVQDIERVNTSPLTRAVTGPELRTIFLGMDQKRDELLYSSVKGKNPFKDKRVRQAFYQAIDIEGIRKTVMR
GASNPSALLVGPGVNGFQADIKRLPYDTDAAKKLLAEAGYPNGFEVGMNCPNDRYVNDARICQAVAANLARIGVKVNLQA
ETKGTYFPRVLRRDTSFYMLGWMPATYDAHNAMTALMACVDDKGAGQFNLGGYCNPKVDQLTRQVQSEVDKARRDAMIRE
AFEIHAADVGHIPLHQQALAWGVSRKVALVQMADNFMPFKWMSLAK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=514, Percent_Identity=24.7081712062257, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI1789966, Length=526, Percent_Identity=24.7148288973384, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1787495, Length=522, Percent_Identity=22.9885057471264, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI87081878, Length=523, Percent_Identity=22.5621414913958, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1789397, Length=519, Percent_Identity=23.5067437379576, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787762, Length=518, Percent_Identity=22.5868725868726, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1789887, Length=465, Percent_Identity=24.0860215053763, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1787551, Length=455, Percent_Identity=23.7362637362637, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58280; Mature: 58280

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFPRTALRAAFGASLIAAACMAGAQTIRVANQGDALSMDPHSLNESLQLSVTGNIYEPL
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCEEEEEECCHHHHH
VGRNKDLSLAPALATRWAQLSPTVWRFELRRGVLFHDGTPFTADDVLFSLARTQAGSSDM
CCCCCCCCHHHHHHHHHHHCCCHHEEHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCCHH
KTYTNDFKEVRKVDDYTVEIETKAPYPILPDVLTLVYMMSRKWCETNHATEPVDRRRGVE
HHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCH
NAASFRANGTGPFRLRERQPGVRTVFTRNAQYWGAIEGNVSEVVFTPIGNDATRVAALLS
HHHHCCCCCCCCEEECCCCCCCEEEEECCCCEEEEECCCCEEEEEECCCCCHHHHHHHHC
GQVDVMEPVPVQDIERVNTSPLTRAVTGPELRTIFLGMDQKRDELLYSSVKGKNPFKDKR
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHH
VRQAFYQAIDIEGIRKTVMRGASNPSALLVGPGVNGFQADIKRLPYDTDAAKKLLAEAGY
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHCCC
PNGFEVGMNCPNDRYVNDARICQAVAANLARIGVKVNLQAETKGTYFPRVLRRDTSFYML
CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHCCCCEEEE
GWMPATYDAHNAMTALMACVDDKGAGQFNLGGYCNPKVDQLTRQVQSEVDKARRDAMIRE
ECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AFEIHAADVGHIPLHQQALAWGVSRKVALVQMADNFMPFKWMSLAK
HHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEHHCCCCCCHHHHHCC
>Mature Secondary Structure
MNFPRTALRAAFGASLIAAACMAGAQTIRVANQGDALSMDPHSLNESLQLSVTGNIYEPL
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCEEEEEECCHHHHH
VGRNKDLSLAPALATRWAQLSPTVWRFELRRGVLFHDGTPFTADDVLFSLARTQAGSSDM
CCCCCCCCHHHHHHHHHHHCCCHHEEHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCCHH
KTYTNDFKEVRKVDDYTVEIETKAPYPILPDVLTLVYMMSRKWCETNHATEPVDRRRGVE
HHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCH
NAASFRANGTGPFRLRERQPGVRTVFTRNAQYWGAIEGNVSEVVFTPIGNDATRVAALLS
HHHHCCCCCCCCEEECCCCCCCEEEEECCCCEEEEECCCCEEEEEECCCCCHHHHHHHHC
GQVDVMEPVPVQDIERVNTSPLTRAVTGPELRTIFLGMDQKRDELLYSSVKGKNPFKDKR
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHH
VRQAFYQAIDIEGIRKTVMRGASNPSALLVGPGVNGFQADIKRLPYDTDAAKKLLAEAGY
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHCCC
PNGFEVGMNCPNDRYVNDARICQAVAANLARIGVKVNLQAETKGTYFPRVLRRDTSFYML
CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHCCCCEEEE
GWMPATYDAHNAMTALMACVDDKGAGQFNLGGYCNPKVDQLTRQVQSEVDKARRDAMIRE
ECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AFEIHAADVGHIPLHQQALAWGVSRKVALVQMADNFMPFKWMSLAK
HHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA