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The map label for this gene is pyrB [H]

Identifier: 120609604

GI number: 120609604

Start: 991465

End: 992427

Strand: Direct

Name: pyrB [H]

Synonym: Aave_0910

Alternate gene names: 120609604

Gene position: 991465-992427 (Clockwise)

Preceding gene: 120609603

Following gene: 120609605

Centisome position: 18.52

GC content: 66.25

Gene sequence:

>963_bases
GTGCTCTCCAAGCGCAACCCCCAGCTCAATCGCAATGGCGAGTTGATCCACCTGCTGTCCATCGAAGGCCTGCCGCGCAG
CATCGTCACGCACATCCTCGATACGGCGGCGAACTTCGTGTCGGTGAACGACCGCGAGGTGAAGAAGGTGCCGCTGCTGC
GCGGCAAGAGCGTGTTCAACCTGTTCTTCGAGAACAGCACGCGCACCCGCACGACCTTCGAGATCGCGGCCAAGCGCCTG
TCGGCCGACGTGCTCAACCTCGACATCGCGCGCTCGTCCGCCAGCAAGGGCGAATCGCTGCTGGACACCATCGCCAACCT
CTCGGCCATGGCGGCAGACCTGTTCGTGGTGCGGCACAGCGAATCGGGCGCGCCCTACCTGATCGCGCAGCACGTGGCGC
CGCACGTACACGTGATCAACGCGGGCGACGGCCGGCATGCGCACCCCACGCAGGGGCTGCTCGACATGTACACCATCCGC
CACTACAAGAAGGATTTCTCCAACCTCACGGTGGCGATCGTGGGCGACGTGCTGCATTCGCGCGTGGCCCGCTCGGACAT
CCACGGCCTGACCACCCTGGGCTGCCCCGAGGTGCGCGTGGTGGGCCCGCGCACCCTGGTGCCCGGAGACCTTTCCCACA
TGGGCGTGCGCGTGTGCCACACGCTGGAAGAGGGCATCAGGGACGCCGACGTGGTCATCATGCTGCGCCTGCAGAACGAG
CGAATGAGCGGCGCGCTGCTGCCCTCCAGCCAGGAATACTTCAAGAGCTTCGGCCTCACGCCCGAGAAGCTGCGCCTGGC
CAAGCCCGACGCCATCGTCATGCACCCCGGGCCGATCAACCGCGGGGTGGAAATCGACTCCGCCGTGGTCGATGGACCGC
AGGCCGTGATCCTGCCCCAGGTGACTTTCGGCATCGCCGTGCGCATGGCCGTGATGTCCATCGTGGCGGGGAATGAGGCT
TAA

Upstream 100 bases:

>100_bases
TTCGCCGCGGCGCGCGTGGCGCTTCCCGCCACGCAGTCACTGGCGCTGGCCCGTGCGGACGGCGGCGCTTTCCACTTCCA
CGTCCAGGAGGCCTGACGCC

Downstream 100 bases:

>100_bases
ACGATGAAGATACTGATCCAGAACGGCCGGGTGATCGATCCGGCGAGCGGGTTCGACAAGACCGTCGATGTGGCCATCGC
CGCGGGGCGGGTGATCGCGG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MLSKRNPQLNRNGELIHLLSIEGLPRSIVTHILDTAANFVSVNDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRL
SADVLNLDIARSSASKGESLLDTIANLSAMAADLFVVRHSESGAPYLIAQHVAPHVHVINAGDGRHAHPTQGLLDMYTIR
HYKKDFSNLTVAIVGDVLHSRVARSDIHGLTTLGCPEVRVVGPRTLVPGDLSHMGVRVCHTLEEGIRDADVVIMLRLQNE
RMSGALLPSSQEYFKSFGLTPEKLRLAKPDAIVMHPGPINRGVEIDSAVVDGPQAVILPQVTFGIAVRMAVMSIVAGNEA

Sequences:

>Translated_320_residues
MLSKRNPQLNRNGELIHLLSIEGLPRSIVTHILDTAANFVSVNDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRL
SADVLNLDIARSSASKGESLLDTIANLSAMAADLFVVRHSESGAPYLIAQHVAPHVHVINAGDGRHAHPTQGLLDMYTIR
HYKKDFSNLTVAIVGDVLHSRVARSDIHGLTTLGCPEVRVVGPRTLVPGDLSHMGVRVCHTLEEGIRDADVVIMLRLQNE
RMSGALLPSSQEYFKSFGLTPEKLRLAKPDAIVMHPGPINRGVEIDSAVVDGPQAVILPQVTFGIAVRMAVMSIVAGNEA
>Mature_320_residues
MLSKRNPQLNRNGELIHLLSIEGLPRSIVTHILDTAANFVSVNDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRL
SADVLNLDIARSSASKGESLLDTIANLSAMAADLFVVRHSESGAPYLIAQHVAPHVHVINAGDGRHAHPTQGLLDMYTIR
HYKKDFSNLTVAIVGDVLHSRVARSDIHGLTTLGCPEVRVVGPRTLVPGDLSHMGVRVCHTLEEGIRDADVVIMLRLQNE
RMSGALLPSSQEYFKSFGLTPEKLRLAKPDAIVMHPGPINRGVEIDSAVVDGPQAVILPQVTFGIAVRMAVMSIVAGNEA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=36.0128617363344, Blast_Score=161, Evalue=7e-40,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=34.9514563106796, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI193204318, Length=337, Percent_Identity=32.0474777448071, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322331, Length=315, Percent_Identity=33.015873015873, Blast_Score=122, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=34.5047923322684, Blast_Score=138, Evalue=5e-33,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34842; Mature: 34842

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKRNPQLNRNGELIHLLSIEGLPRSIVTHILDTAANFVSVNDREVKKVPLLRGKSVFN
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHEEECCCCHHHHCCCCCCCCEEE
LFFENSTRTRTTFEIAAKRLSADVLNLDIARSSASKGESLLDTIANLSAMAADLFVVRHS
EEECCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEEC
ESGAPYLIAQHVAPHVHVINAGDGRHAHPTQGLLDMYTIRHYKKDFSNLTVAIVGDVLHS
CCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCEEHHHHHHHHHH
RVARSDIHGLTTLGCPEVRVVGPRTLVPGDLSHMGVRVCHTLEEGIRDADVVIMLRLQNE
HHHHHHHCCCEECCCCCEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
RMSGALLPSSQEYFKSFGLTPEKLRLAKPDAIVMHPGPINRGVEIDSAVVDGPQAVILPQ
CCCCCCCCCCHHHHHHHCCCHHHEEECCCCEEEECCCCCCCCCEECHHEECCCCEEEECH
VTFGIAVRMAVMSIVAGNEA
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSKRNPQLNRNGELIHLLSIEGLPRSIVTHILDTAANFVSVNDREVKKVPLLRGKSVFN
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHEEECCCCHHHHCCCCCCCCEEE
LFFENSTRTRTTFEIAAKRLSADVLNLDIARSSASKGESLLDTIANLSAMAADLFVVRHS
EEECCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEEC
ESGAPYLIAQHVAPHVHVINAGDGRHAHPTQGLLDMYTIRHYKKDFSNLTVAIVGDVLHS
CCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCEEHHHHHHHHHH
RVARSDIHGLTTLGCPEVRVVGPRTLVPGDLSHMGVRVCHTLEEGIRDADVVIMLRLQNE
HHHHHHHCCCEECCCCCEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
RMSGALLPSSQEYFKSFGLTPEKLRLAKPDAIVMHPGPINRGVEIDSAVVDGPQAVILPQ
CCCCCCCCCCHHHHHHHCCCHHHEEECCCCEEEECCCCCCCCCEECHHEECCCCEEEECH
VTFGIAVRMAVMSIVAGNEA
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA