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The map label for this gene is pyrC' [H]

Identifier: 120609605

GI number: 120609605

Start: 992431

End: 993726

Strand: Direct

Name: pyrC' [H]

Synonym: Aave_0911

Alternate gene names: 120609605

Gene position: 992431-993726 (Clockwise)

Preceding gene: 120609604

Following gene: 120609606

Centisome position: 18.54

GC content: 72.69

Gene sequence:

>1296_bases
ATGAAGATACTGATCCAGAACGGCCGGGTGATCGATCCGGCGAGCGGGTTCGACAAGACCGTCGATGTGGCCATCGCCGC
GGGGCGGGTGATCGCGGTCGGCCGCGCTCCCCAGGACTTCCATCCGGCGCGCACGATCGACGCCACGGACTGCATCGTGC
TGCCGGGGCTGGTGGACCTCGCCGCCCGCCTGCGCGAGCCCGGGCACGAGCACGAGGGCATGCTGGAGTCCGAGATGGCC
GCGGCCGTGGCCGGCGGCGTCACCAGCCTCGTGTGCCCGCCCGATACCGATCCGGTGCTCGACGAGCCCGGCCTCGTGGA
GATGCTCAAGTTCCGCGCCGAGAAGCTGCACCGGGCGCGCCTCTTCCCCCTGGGCGCGCTCACGCGCGGACTGGCCGGCG
AGGTGCTGACCGAGATGGCGGAACTCACCGAATCCGGCTGCGTGGGTTTCGGCCAGGCCGACGTGGCGCTCGCCAGCACC
CAGGTGCTGCAGCGCGCGCTGCAGTATGCCGCCACCTACGGCTACACGGTCTGGCTGCGGCCGCAGGAGCTCCACCTGGG
CAAGGGCGTGGCCGCCAGCGGCCCGCTCGCGACACGGCTGGGCCTGTCGGGCATTCCCGTGGCGGCCGAGACCATCGCGC
TGCACACCATCTTCGAACTGCTCAAGCCCATCGGCGCGCGCGTCCACCTGTGCCGGCTCTCCAGCGCGGCGGGCGTGCAA
CTGGTGCGGCAGGCGAAGGCCGAGGGCCTGCAGGTCACCTGCGATGTGAGCATCAACTCGCTGCTGCTCACCGACACCGA
TATCGGCTATTTCGACAGCCGCGCCCGCCTGTCGCCGCCGCTGCGCCAGCAGCGCGACCGCGACGCCCTGCTGGCCGCGC
TGGCCGACGGCACCATCGATGCGCTCGTGTCCGACCACACGCCGGTGGACGAGGACGCCAAGACGCTGCCCTTCGCCGAA
TCCGAGCCCGGCGCCACCGGCCTGGAACTGCTGGCGAGCATCGCGCTCAAGTGGTCCCGCGACCATGGCGTGCCGCTGGA
CCGGGCGTTCGCCACGGTGACCTCCGCGCCTGCGCGCGTGCTGGGTTCCGCGCTCGGCACCTTCGCCGCCAGCGCCGGCC
GGCTCGTGGAGGGCGGCGTGGCCGACCTCTGCGTGTTCGATCCCCAGGCCGCCTGGACCGTGGAGCCCGGGGCACTGCGC
AGCCAGGGGCGGCACACGCCGTTCTCCGGCTACGAGCTGCCGGGCCGCGTGCGCTGGACGCTGGTGGGCGGCATCGTCGC
GTTCGAGCGCGTCTGA

Upstream 100 bases:

>100_bases
GGTCGATGGACCGCAGGCCGTGATCCTGCCCCAGGTGACTTTCGGCATCGCCGTGCGCATGGCCGTGATGTCCATCGTGG
CGGGGAATGAGGCTTAAACG

Downstream 100 bases:

>100_bases
TGCGCTCGCTGCGCGCGGTGGCGCGCCTGATGCGGCTCCTGGCGCACATCCTGGCGGGGCTGGCCACGGTGCTGCTGCGC
TTCCCCCGCATGGGGCCGGA

Product: dihydroorotase

Products: Allantoate; H+ [C]

Alternate protein names: Aspartate carbamoyltransferase 44 kDa Non-catalytic chain [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKILIQNGRVIDPASGFDKTVDVAIAAGRVIAVGRAPQDFHPARTIDATDCIVLPGLVDLAARLREPGHEHEGMLESEMA
AAVAGGVTSLVCPPDTDPVLDEPGLVEMLKFRAEKLHRARLFPLGALTRGLAGEVLTEMAELTESGCVGFGQADVALAST
QVLQRALQYAATYGYTVWLRPQELHLGKGVAASGPLATRLGLSGIPVAAETIALHTIFELLKPIGARVHLCRLSSAAGVQ
LVRQAKAEGLQVTCDVSINSLLLTDTDIGYFDSRARLSPPLRQQRDRDALLAALADGTIDALVSDHTPVDEDAKTLPFAE
SEPGATGLELLASIALKWSRDHGVPLDRAFATVTSAPARVLGSALGTFAASAGRLVEGGVADLCVFDPQAAWTVEPGALR
SQGRHTPFSGYELPGRVRWTLVGGIVAFERV

Sequences:

>Translated_431_residues
MKILIQNGRVIDPASGFDKTVDVAIAAGRVIAVGRAPQDFHPARTIDATDCIVLPGLVDLAARLREPGHEHEGMLESEMA
AAVAGGVTSLVCPPDTDPVLDEPGLVEMLKFRAEKLHRARLFPLGALTRGLAGEVLTEMAELTESGCVGFGQADVALAST
QVLQRALQYAATYGYTVWLRPQELHLGKGVAASGPLATRLGLSGIPVAAETIALHTIFELLKPIGARVHLCRLSSAAGVQ
LVRQAKAEGLQVTCDVSINSLLLTDTDIGYFDSRARLSPPLRQQRDRDALLAALADGTIDALVSDHTPVDEDAKTLPFAE
SEPGATGLELLASIALKWSRDHGVPLDRAFATVTSAPARVLGSALGTFAASAGRLVEGGVADLCVFDPQAAWTVEPGALR
SQGRHTPFSGYELPGRVRWTLVGGIVAFERV
>Mature_431_residues
MKILIQNGRVIDPASGFDKTVDVAIAAGRVIAVGRAPQDFHPARTIDATDCIVLPGLVDLAARLREPGHEHEGMLESEMA
AAVAGGVTSLVCPPDTDPVLDEPGLVEMLKFRAEKLHRARLFPLGALTRGLAGEVLTEMAELTESGCVGFGQADVALAST
QVLQRALQYAATYGYTVWLRPQELHLGKGVAASGPLATRLGLSGIPVAAETIALHTIFELLKPIGARVHLCRLSSAAGVQ
LVRQAKAEGLQVTCDVSINSLLLTDTDIGYFDSRARLSPPLRQQRDRDALLAALADGTIDALVSDHTPVDEDAKTLPFAE
SEPGATGLELLASIALKWSRDHGVPLDRAFATVTSAPARVLGSALGTFAASAGRLVEGGVADLCVFDPQAAWTVEPGALR
SQGRHTPFSGYELPGRVRWTLVGGIVAFERV

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. PyrC' subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=389, Percent_Identity=26.4781491002571, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI19923821, Length=463, Percent_Identity=23.9740820734341, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1786722, Length=445, Percent_Identity=25.8426966292135, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87082175, Length=454, Percent_Identity=25.1101321585903, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6322218, Length=408, Percent_Identity=25, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859883, Length=220, Percent_Identity=29.0909090909091, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.5 [C]

Molecular weight: Translated: 45471; Mature: 45471

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIQNGRVIDPASGFDKTVDVAIAAGRVIAVGRAPQDFHPARTIDATDCIVLPGLVDL
CEEEEECCCEECCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCEEECCCHHHH
AARLREPGHEHEGMLESEMAAAVAGGVTSLVCPPDTDPVLDEPGLVEMLKFRAEKLHRAR
HHHHHCCCCHHCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LFPLGALTRGLAGEVLTEMAELTESGCVGFGQADVALASTQVLQRALQYAATYGYTVWLR
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEE
PQELHLGKGVAASGPLATRLGLSGIPVAAETIALHTIFELLKPIGARVHLCRLSSAAGVQ
CHHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHH
LVRQAKAEGLQVTCDVSINSLLLTDTDIGYFDSRARLSPPLRQQRDRDALLAALADGTID
HHHHHHHCCEEEEEEEEECEEEEEECCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCHHH
ALVSDHTPVDEDAKTLPFAESEPGATGLELLASIALKWSRDHGVPLDRAFATVTSAPARV
HHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
LGSALGTFAASAGRLVEGGVADLCVFDPQAAWTVEPGALRSQGRHTPFSGYELPGRVRWT
HHHHHHHHHHCCCCEECCCCCEEEEECCCCCEEECCCHHHCCCCCCCCCCCCCCCEEEEE
LVGGIVAFERV
EEEEEHHHCCC
>Mature Secondary Structure
MKILIQNGRVIDPASGFDKTVDVAIAAGRVIAVGRAPQDFHPARTIDATDCIVLPGLVDL
CEEEEECCCEECCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCEEECCCHHHH
AARLREPGHEHEGMLESEMAAAVAGGVTSLVCPPDTDPVLDEPGLVEMLKFRAEKLHRAR
HHHHHCCCCHHCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LFPLGALTRGLAGEVLTEMAELTESGCVGFGQADVALASTQVLQRALQYAATYGYTVWLR
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEE
PQELHLGKGVAASGPLATRLGLSGIPVAAETIALHTIFELLKPIGARVHLCRLSSAAGVQ
CHHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHH
LVRQAKAEGLQVTCDVSINSLLLTDTDIGYFDSRARLSPPLRQQRDRDALLAALADGTID
HHHHHHHCCEEEEEEEEECEEEEEECCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCHHH
ALVSDHTPVDEDAKTLPFAESEPGATGLELLASIALKWSRDHGVPLDRAFATVTSAPARV
HHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
LGSALGTFAASAGRLVEGGVADLCVFDPQAAWTVEPGALRSQGRHTPFSGYELPGRVRWT
HHHHHHHHHHCCCCEECCCCCEEEEECCCCCEEECCCHHHCCCCCCCCCCCCCCCEEEEE
LVGGIVAFERV
EEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Allantoin; H2O [C]

Specific reaction: Allantoin + H2O --> Allantoate + H+ [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; p-Chloromercuribenzene-sulphonic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]