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The map label for this gene is garL [H]

Identifier: 120609532

GI number: 120609532

Start: 908333

End: 909142

Strand: Direct

Name: garL [H]

Synonym: Aave_0838

Alternate gene names: 120609532

Gene position: 908333-909142 (Clockwise)

Preceding gene: 120609531

Following gene: 120609533

Centisome position: 16.97

GC content: 74.32

Gene sequence:

>810_bases
ATGAGCGGCATCCTCCATTCCCTGCCTTCCGGTCCGTCCCCCGCGGCACGGCTGCGCAGCCGCATCCTGGCGCGCGACCG
CCTGATGGCGACCTTCGTGAAGACGACCTCGCACCAGACGGTGGAGGTGCTGGCCGCCACGGGGATGGACACGCTCGTGC
TCGACGCCGAGCACGCGCCGTTCGGTCCGGAGAGCCTGGACCGCAGCCTGCTCGCCGCCCATGCCTGCGCGCTGCCGGTC
CTGGTCCGCGTGCCCTATCCGCGCGCGGCGGCCATACAGCAGGCGCTGGACCTCGGCGCGGGCGGGGTGATGGTGCCGCA
TGTGGACGATGCCCGCATCGCCGAAGAGGTGGTGCGCGCGGCGCGCTACGGCCAGGGCGGAGCCCGGGGCTTCAGCAATT
CCTCGCGCGCCGGGGGCTACGGCGCCCGCGGCATGGCCGAGCACCTGCTCGCCAGCGACGCGCACACCTCGGTCATCGTG
CAGATCGAAAGCGCCCGCGCCGTGGCCGAGGCGGCCGCCATCGCGGCCGTGCCGGGCGTCGATTGCCTGTTCGTCGGCCC
GGCCGACCTGGCCGTTTCCCTCGGAGCCCAGGGGCTGGACGATCCGCGCGTGGCCGCCGCGGTCGCGGCCGTGAGCGCGG
CCGGGCTGGCCCAGGGGCGCGCAGTCGGCTGCTTCGTGCCCGACGTGCACGGCATTCCTCCGCTGGCCGCGCAAGGCATA
TCGTTTTTCGTGGTCGGCTCCGACCAGGCCCTCCTGCGCAGGGCTGCGCGGCAGGTGCTGGCCGAGGCGACCGAATCCGA
GGCGGCCTGA

Upstream 100 bases:

>100_bases
TGCTCATTCCTCCCCATGCGCTGCACTACGGCGTGGTGGTGGGCGACGAGCCCGTGCTCAACCTGGACGTCTTCACGCCC
GCGCGGCCGGAATACCTCGC

Downstream 100 bases:

>100_bases
AGGCATCGGGAAACCTGCCCATGGACCACCTTATTTCCGTTTCTCCAGCGCGCGTGACCGGGGGCCCGCGCGCAATTGCG
TTCCCTGAATGCCCCCCGCA

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSGILHSLPSGPSPAARLRSRILARDRLMATFVKTTSHQTVEVLAATGMDTLVLDAEHAPFGPESLDRSLLAAHACALPV
LVRVPYPRAAAIQQALDLGAGGVMVPHVDDARIAEEVVRAARYGQGGARGFSNSSRAGGYGARGMAEHLLASDAHTSVIV
QIESARAVAEAAAIAAVPGVDCLFVGPADLAVSLGAQGLDDPRVAAAVAAVSAAGLAQGRAVGCFVPDVHGIPPLAAQGI
SFFVVGSDQALLRRAARQVLAEATESEAA

Sequences:

>Translated_269_residues
MSGILHSLPSGPSPAARLRSRILARDRLMATFVKTTSHQTVEVLAATGMDTLVLDAEHAPFGPESLDRSLLAAHACALPV
LVRVPYPRAAAIQQALDLGAGGVMVPHVDDARIAEEVVRAARYGQGGARGFSNSSRAGGYGARGMAEHLLASDAHTSVIV
QIESARAVAEAAAIAAVPGVDCLFVGPADLAVSLGAQGLDDPRVAAAVAAVSAAGLAQGRAVGCFVPDVHGIPPLAAQGI
SFFVVGSDQALLRRAARQVLAEATESEAA
>Mature_268_residues
SGILHSLPSGPSPAARLRSRILARDRLMATFVKTTSHQTVEVLAATGMDTLVLDAEHAPFGPESLDRSLLAAHACALPVL
VRVPYPRAAAIQQALDLGAGGVMVPHVDDARIAEEVVRAARYGQGGARGFSNSSRAGGYGARGMAEHLLASDAHTSVIVQ
IESARAVAEAAAIAAVPGVDCLFVGPADLAVSLGAQGLDDPRVAAAVAAVSAAGLAQGRAVGCFVPDVHGIPPLAAQGIS
FFVVGSDQALLRRAARQVLAEATESEAA

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=244, Percent_Identity=32.7868852459016, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1788578, Length=261, Percent_Identity=30.6513409961686, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 27342; Mature: 27211

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGILHSLPSGPSPAARLRSRILARDRLMATFVKTTSHQTVEVLAATGMDTLVLDAEHAP
CCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCC
FGPESLDRSLLAAHACALPVLVRVPYPRAAAIQQALDLGAGGVMVPHVDDARIAEEVVRA
CCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHH
ARYGQGGARGFSNSSRAGGYGARGMAEHLLASDAHTSVIVQIESARAVAEAAAIAAVPGV
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCC
DCLFVGPADLAVSLGAQGLDDPRVAAAVAAVSAAGLAQGRAVGCFVPDVHGIPPLAAQGI
CEEEECHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCC
SFFVVGSDQALLRRAARQVLAEATESEAA
EEEEECCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGILHSLPSGPSPAARLRSRILARDRLMATFVKTTSHQTVEVLAATGMDTLVLDAEHAP
CCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCC
FGPESLDRSLLAAHACALPVLVRVPYPRAAAIQQALDLGAGGVMVPHVDDARIAEEVVRA
CCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHH
ARYGQGGARGFSNSSRAGGYGARGMAEHLLASDAHTSVIVQIESARAVAEAAAIAAVPGV
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCC
DCLFVGPADLAVSLGAQGLDDPRVAAAVAAVSAAGLAQGRAVGCFVPDVHGIPPLAAQGI
CEEEECHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCC
SFFVVGSDQALLRRAARQVLAEATESEAA
EEEEECCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA