Click here to switch to the map view.

The map label for this gene is rlmL [H]

Identifier: 120609125

GI number: 120609125

Start: 455047

End: 456372

Strand: Direct

Name: rlmL [H]

Synonym: Aave_0422

Alternate gene names: 120609125

Gene position: 455047-456372 (Clockwise)

Preceding gene: 120609122

Following gene: 120609126

Centisome position: 8.5

GC content: 71.64

Gene sequence:

>1326_bases
ATGAATTCACTGCAAATCTTTCTGCCTTGCGCCGCCGGCGTGGAAGGCTTCCTGGCCGACGAGGTCCATGGCATCACCGG
CCTGACGGGCCACGACCTGCTCACGGGGCGCGGCGGCGCGATGGCCCGCGCGTCGTGGCGCGAGGCGCTGCGGCTGAACC
TTCACAGCCGCCTCGCGCAACGGGTGCTGGTGCACCTGGCCGAGCGGATGTACCGCTCCGAGAACGATCTCTACGCCATC
GCGTCCGGGATCGCCTGGGAGATCTGGTTCACTCCGCGCCAAAGCTTCCGTATCGATGTCACTGCCCAGCACAGCCCGCT
CCAGAGCCTGAATTTCGCGGCACTGCGCGTGAAGGACGCGGTCGCCGACCGTTTCCGCGCGAAAGCCGGCGGCGTGCGGC
CCGACGTGGACACGCAGCGGCCGGACGTGCGCATCCATCTGCACCTCACCACGGACCAGGCCACGGTCTATATCGACACC
TCGGGCGAGCCGCTCTTCAAGCGCGGCTGGCGGGAGGACAAGGGGGATGCGCCGCTCAAGGAAACGCTCGCCGCGGCCAT
GATCGCCGCCAGCGGCTGGGACCCGCTGGGCGACACGCCCGTGCCGCTGTACGACCCCTGCTGCGGCAGCGGCACGATCG
CCATCGAGGCCGCGCAGATGGCCTGCCGCATTCCCCCCGGCCTGCGCCGGCGCTTCGCCTTCGAGAAACTGCTGCCCTTC
CAGGCGCACGTCTGGAATGCGCTGCGAGAGGAAGCGCAGGACGGCATCGTGCCGGCCGCCGTGCCGGTGTTCGGCAGCGA
CGTGTCGCACCGCATGGTGGATTTCGCGCAGCGCAATGCCGAGCGCGCGGGCGTCGCCCAGGCGGTGCAGTTGCGCGGCG
GAGACGCGCTGCAGCGCATGCCGCCCGGCGACCGGCCCGGCGTGATGCTGCTCAACCCGCCCTACGGCGAGCGCATCGCC
GCGGCCGGCACGGCGGGGCGCAGCGCGCGGGAGCGCATGGGCGTGGTCGAGCGCGCCGGCCGGGAGACCGCGCAGACCGA
GGATGGCGGGGATTTCTTCCTGCAGCTCGCCGCGCACTGGAAGAAGCACTATGCGGGCTGGCAGGCCTGGATGCTCACGC
CCGACATGAAGCTGCCCGGCAAGATGCGGCTGAAGGAGTCGCGCCGCGTACCGATGTGGAACGGGCCGATCGAATGCCGG
CTGTTCCGCTTCGACATGGTGCAGGGCGCCGTGCGCCCCCGGGCGGAGGGCGCGCCGCCGCCGCCGGCGGATGCGCCTGC
CGGCCCCGGAAGCGCCGCGGACGCGGGCCGGAACCATGCCGGTTGA

Upstream 100 bases:

>100_bases
TGCTGAACTTTATCAACAGAATGAAACATTTGTTCTGCATGGTGGAAAAATAAAGGCGGGGGATTGCATTGGCGGCAAGG
CGTATCCTCTCGCCTGCGTT

Downstream 100 bases:

>100_bases
ACTGTGCCTGCCCGCGCGGGCCGCGGAGGGTGAACCGGCGCGCTGCGTGGTGGTGGACACCAACGTGGCCCTGGACCTTC
TGATCTTTTCCGATCCGCGC

Product: putative RNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MNSLQIFLPCAAGVEGFLADEVHGITGLTGHDLLTGRGGAMARASWREALRLNLHSRLAQRVLVHLAERMYRSENDLYAI
ASGIAWEIWFTPRQSFRIDVTAQHSPLQSLNFAALRVKDAVADRFRAKAGGVRPDVDTQRPDVRIHLHLTTDQATVYIDT
SGEPLFKRGWREDKGDAPLKETLAAAMIAASGWDPLGDTPVPLYDPCCGSGTIAIEAAQMACRIPPGLRRRFAFEKLLPF
QAHVWNALREEAQDGIVPAAVPVFGSDVSHRMVDFAQRNAERAGVAQAVQLRGGDALQRMPPGDRPGVMLLNPPYGERIA
AAGTAGRSARERMGVVERAGRETAQTEDGGDFFLQLAAHWKKHYAGWQAWMLTPDMKLPGKMRLKESRRVPMWNGPIECR
LFRFDMVQGAVRPRAEGAPPPPADAPAGPGSAADAGRNHAG

Sequences:

>Translated_441_residues
MNSLQIFLPCAAGVEGFLADEVHGITGLTGHDLLTGRGGAMARASWREALRLNLHSRLAQRVLVHLAERMYRSENDLYAI
ASGIAWEIWFTPRQSFRIDVTAQHSPLQSLNFAALRVKDAVADRFRAKAGGVRPDVDTQRPDVRIHLHLTTDQATVYIDT
SGEPLFKRGWREDKGDAPLKETLAAAMIAASGWDPLGDTPVPLYDPCCGSGTIAIEAAQMACRIPPGLRRRFAFEKLLPF
QAHVWNALREEAQDGIVPAAVPVFGSDVSHRMVDFAQRNAERAGVAQAVQLRGGDALQRMPPGDRPGVMLLNPPYGERIA
AAGTAGRSARERMGVVERAGRETAQTEDGGDFFLQLAAHWKKHYAGWQAWMLTPDMKLPGKMRLKESRRVPMWNGPIECR
LFRFDMVQGAVRPRAEGAPPPPADAPAGPGSAADAGRNHAG
>Mature_441_residues
MNSLQIFLPCAAGVEGFLADEVHGITGLTGHDLLTGRGGAMARASWREALRLNLHSRLAQRVLVHLAERMYRSENDLYAI
ASGIAWEIWFTPRQSFRIDVTAQHSPLQSLNFAALRVKDAVADRFRAKAGGVRPDVDTQRPDVRIHLHLTTDQATVYIDT
SGEPLFKRGWREDKGDAPLKETLAAAMIAASGWDPLGDTPVPLYDPCCGSGTIAIEAAQMACRIPPGLRRRFAFEKLLPF
QAHVWNALREEAQDGIVPAAVPVFGSDVSHRMVDFAQRNAERAGVAQAVQLRGGDALQRMPPGDRPGVMLLNPPYGERIA
AAGTAGRSARERMGVVERAGRETAQTEDGGDFFLQLAAHWKKHYAGWQAWMLTPDMKLPGKMRLKESRRVPMWNGPIECR
LFRFDMVQGAVRPRAEGAPPPPADAPAGPGSAADAGRNHAG

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=372, Percent_Identity=31.1827956989247, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 48175; Mature: 48175

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLQIFLPCAAGVEGFLADEVHGITGLTGHDLLTGRGGAMARASWREALRLNLHSRLAQ
CCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHH
RVLVHLAERMYRSENDLYAIASGIAWEIWFTPRQSFRIDVTAQHSPLQSLNFAALRVKDA
HHHHHHHHHHHCCCCCEEEEECCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHH
VADRFRAKAGGVRPDVDTQRPDVRIHLHLTTDQATVYIDTSGEPLFKRGWREDKGDAPLK
HHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCHHHHCCCCCCCCCCCHH
ETLAAAMIAASGWDPLGDTPVPLYDPCCGSGTIAIEAAQMACRIPPGLRRRFAFEKLLPF
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHCCH
QAHVWNALREEAQDGIVPAAVPVFGSDVSHRMVDFAQRNAERAGVAQAVQLRGGDALQRM
HHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHCC
PPGDRPGVMLLNPPYGERIAAAGTAGRSARERMGVVERAGRETAQTEDGGDFFLQLAAHW
CCCCCCCEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHH
KKHYAGWQAWMLTPDMKLPGKMRLKESRRVPMWNGPIECRLFRFDMVQGAVRPRAEGAPP
HHHHCCCEEEEECCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEEHHHHHCCCCCCCCCCC
PPADAPAGPGSAADAGRNHAG
CCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSLQIFLPCAAGVEGFLADEVHGITGLTGHDLLTGRGGAMARASWREALRLNLHSRLAQ
CCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHH
RVLVHLAERMYRSENDLYAIASGIAWEIWFTPRQSFRIDVTAQHSPLQSLNFAALRVKDA
HHHHHHHHHHHCCCCCEEEEECCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHH
VADRFRAKAGGVRPDVDTQRPDVRIHLHLTTDQATVYIDTSGEPLFKRGWREDKGDAPLK
HHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCHHHHCCCCCCCCCCCHH
ETLAAAMIAASGWDPLGDTPVPLYDPCCGSGTIAIEAAQMACRIPPGLRRRFAFEKLLPF
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHCCH
QAHVWNALREEAQDGIVPAAVPVFGSDVSHRMVDFAQRNAERAGVAQAVQLRGGDALQRM
HHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHCC
PPGDRPGVMLLNPPYGERIAAAGTAGRSARERMGVVERAGRETAQTEDGGDFFLQLAAHW
CCCCCCCEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHH
KKHYAGWQAWMLTPDMKLPGKMRLKESRRVPMWNGPIECRLFRFDMVQGAVRPRAEGAPP
HHHHCCCEEEEECCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEEHHHHHCCCCCCCCCCC
PPADAPAGPGSAADAGRNHAG
CCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA