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The map label for this gene is rpoN [H]

Identifier: 120609122

GI number: 120609122

Start: 450819

End: 452408

Strand: Direct

Name: rpoN [H]

Synonym: Aave_0419

Alternate gene names: 120609122

Gene position: 450819-452408 (Clockwise)

Preceding gene: 120609121

Following gene: 120609125

Centisome position: 8.42

GC content: 70.06

Gene sequence:

>1590_bases
ATGAAGCCGGGACTGTCGCTGCGCATCTCGCAGCATCTGGCGCTGACGCCGCAACTGCAGCAATCGATCCGGCTGCTGCA
GCTGTCCACGCTGGAGCTGTCGCAGGAAGTCGAGCAGATGCTCGACGAGAACCCCTTCCTGGAGCGCAACCCGGACGAGG
CACCGCGCGAGGAGTTCGGGCTGCTGCAGGCGGATGCGCCGGTGAGCGACGCCGACCGCGGGGCGGAGGAGTCCCTCTAT
TCGCCGGCCAGCGGGGCGGTGGAGACGGCGGCCTCGGCGCCGGGCGAGGGCGCATCCGAAGGATCGGAGGCGCCGGAAGC
TCCCGATGCGCCGGACTGGGAGGGCGACGGCACCGTCGAGATGGCGCCCGACGACGGCGAGTGGGGCGGCGAGGCGCCCG
CGCGCACGCGCAACCAGCTGGGGGACGAAGACGACACGGACGCGACCGAACTCGCGCGCGAGCACGAGTCGCTCACCGAT
TACCTGCACCGGCAGGCGCTGTCGCTGCGCCTGTCGGAGGTGGACCGCGCGGCGCTGCGCTTCCTGATCGAATCGCTGAA
CGACGACGGCTACCTCGACGAATCGCTGGAGGCACTGGCCGTGGCCCTCGCGGGCGAGGAAGACCCCGAGCAGGTGGAGG
AACTGGTGCATCGCTTCACGGTGGCGCTGCGGCTGCTGCAGAGCCTGGAGCCCGTGGGCGTGGGCGCGCGCGGCCTGAGC
GAATGCCTGGCGCTGCAGCTGCGGGCCCTGCAGGAAGATGGCGACGCGGAGCCCGAAGTGCTGCAGGCGGCGCTGCGCAT
CTGCAATCAGCCGCTGGAGATGCTGGCGCGGCGCGACGTGCGCCGGCTCGCGCAGGGTTGCGGTGCCGGCGAGGAGTGCA
CGCGCGCGGCGATGGCGCTCATCGCGCGGCTGGAGCCCCGGCCGGGCCGGGCTTTCGCGCAGGTCGAGCGCAACATCATC
GTGCCCGACGTGATCGTGAAGAAGAGCAGTGGCCGCGGGGCCGCGCATGCGTTCACCGTGCAGCTCAACCCGGATGTCAT
GCCGCGGCTGCGCGTGCACGACATCTATGCCGGGGCGCTGCGCGGCAACCGCGGCGGCGAGGGGCACCAGGCGCTGCAGC
AGCGGCTGCAGGAAGCGCGTTGGTTCATCAAGAACATCCAGCAGCGGTTCGACACCATCCTGCGCGTCTCGCGCGCCATC
GTGGACCGGCAGAAGAACTTCTTCGTGCATGGCGAGCTGGCCATGCGGCCGCTCGTGCTGCGCGACATCGCCGACGAACT
GGGGCTGCACGAATCGACGATCAGCCGGGTGACCACGGCCAAGTACATGGCCACGCCCCAGGGTACCTACGAGCTGAAGT
ATTTCTTCGGCTCGGGCCTGGGCACGGAAACGGGCGGCAATGCGTCGAGCACGGCCGTGCGTGCGCTGATCAAGCAGTTC
GTCGCGTCGGAGAGCCCGGCCAAGCCGCTGTCGGACAGCCAGATCGCGGAGATGCTCAAGGAGCAGGGCATCGAATGCGC
GCGCCGCACGGTGGCGAAATACCGCGAGGCGCTGAAGATCGCACCGGCCAACCTGCGCAAGGCGCTGTAA

Upstream 100 bases:

>100_bases
TCATCAGCGACGGTCGGGTGCTCGCGCAGGGTACTCCCTCGGAGATCGTGGACAACGCAGAAGTGCGCCGCGTGTACCTC
GGCGAACACTTCCGCATGTG

Downstream 100 bases:

>100_bases
AGGCCGGCCGCCGAGGGGCGGCATTCAGCCGAGCAGGCCAGGATTCATGCGCCAGAGGGTGTAGTTGAGGGCTCCGGCGA
AACTGATCCAGGCCAGGTAG

Product: RNA polymerase sigma-54 subunit RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MKPGLSLRISQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERNPDEAPREEFGLLQADAPVSDADRGAEESLY
SPASGAVETAASAPGEGASEGSEAPEAPDAPDWEGDGTVEMAPDDGEWGGEAPARTRNQLGDEDDTDATELAREHESLTD
YLHRQALSLRLSEVDRAALRFLIESLNDDGYLDESLEALAVALAGEEDPEQVEELVHRFTVALRLLQSLEPVGVGARGLS
ECLALQLRALQEDGDAEPEVLQAALRICNQPLEMLARRDVRRLAQGCGAGEECTRAAMALIARLEPRPGRAFAQVERNII
VPDVIVKKSSGRGAAHAFTVQLNPDVMPRLRVHDIYAGALRGNRGGEGHQALQQRLQEARWFIKNIQQRFDTILRVSRAI
VDRQKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQF
VASESPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Sequences:

>Translated_529_residues
MKPGLSLRISQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERNPDEAPREEFGLLQADAPVSDADRGAEESLY
SPASGAVETAASAPGEGASEGSEAPEAPDAPDWEGDGTVEMAPDDGEWGGEAPARTRNQLGDEDDTDATELAREHESLTD
YLHRQALSLRLSEVDRAALRFLIESLNDDGYLDESLEALAVALAGEEDPEQVEELVHRFTVALRLLQSLEPVGVGARGLS
ECLALQLRALQEDGDAEPEVLQAALRICNQPLEMLARRDVRRLAQGCGAGEECTRAAMALIARLEPRPGRAFAQVERNII
VPDVIVKKSSGRGAAHAFTVQLNPDVMPRLRVHDIYAGALRGNRGGEGHQALQQRLQEARWFIKNIQQRFDTILRVSRAI
VDRQKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQF
VASESPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL
>Mature_529_residues
MKPGLSLRISQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERNPDEAPREEFGLLQADAPVSDADRGAEESLY
SPASGAVETAASAPGEGASEGSEAPEAPDAPDWEGDGTVEMAPDDGEWGGEAPARTRNQLGDEDDTDATELAREHESLTD
YLHRQALSLRLSEVDRAALRFLIESLNDDGYLDESLEALAVALAGEEDPEQVEELVHRFTVALRLLQSLEPVGVGARGLS
ECLALQLRALQEDGDAEPEVLQAALRICNQPLEMLARRDVRRLAQGCGAGEECTRAAMALIARLEPRPGRAFAQVERNII
VPDVIVKKSSGRGAAHAFTVQLNPDVMPRLRVHDIYAGALRGNRGGEGHQALQQRLQEARWFIKNIQQRFDTILRVSRAI
VDRQKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQF
VASESPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=532, Percent_Identity=41.1654135338346, Blast_Score=354, Evalue=1e-98,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 57888; Mature: 57888

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPGLSLRISQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERNPDEAPREEFG
CCCCCCEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHC
LLQADAPVSDADRGAEESLYSPASGAVETAASAPGEGASEGSEAPEAPDAPDWEGDGTVE
CCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
MAPDDGEWGGEAPARTRNQLGDEDDTDATELAREHESLTDYLHRQALSLRLSEVDRAALR
ECCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLIESLNDDGYLDESLEALAVALAGEEDPEQVEELVHRFTVALRLLQSLEPVGVGARGLS
HHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ECLALQLRALQEDGDAEPEVLQAALRICNQPLEMLARRDVRRLAQGCGAGEECTRAAMAL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IARLEPRPGRAFAQVERNIIVPDVIVKKSSGRGAAHAFTVQLNPDVMPRLRVHDIYAGAL
HHHCCCCCCHHHHHHHCCCCCCCCEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHH
RGNRGGEGHQALQQRLQEARWFIKNIQQRFDTILRVSRAIVDRQKNFFVHGELAMRPLVL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHH
RDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQF
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
VASESPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL
HCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MKPGLSLRISQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERNPDEAPREEFG
CCCCCCEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHC
LLQADAPVSDADRGAEESLYSPASGAVETAASAPGEGASEGSEAPEAPDAPDWEGDGTVE
CCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
MAPDDGEWGGEAPARTRNQLGDEDDTDATELAREHESLTDYLHRQALSLRLSEVDRAALR
ECCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLIESLNDDGYLDESLEALAVALAGEEDPEQVEELVHRFTVALRLLQSLEPVGVGARGLS
HHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ECLALQLRALQEDGDAEPEVLQAALRICNQPLEMLARRDVRRLAQGCGAGEECTRAAMAL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IARLEPRPGRAFAQVERNIIVPDVIVKKSSGRGAAHAFTVQLNPDVMPRLRVHDIYAGAL
HHHCCCCCCHHHHHHHCCCCCCCCEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHH
RGNRGGEGHQALQQRLQEARWFIKNIQQRFDTILRVSRAIVDRQKNFFVHGELAMRPLVL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHH
RDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQF
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
VASESPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL
HCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]