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The map label for this gene is dmlR [H]

Identifier: 120609112

GI number: 120609112

Start: 440503

End: 441480

Strand: Direct

Name: dmlR [H]

Synonym: Aave_0409

Alternate gene names: 120609112

Gene position: 440503-441480 (Clockwise)

Preceding gene: 120609103

Following gene: 120609113

Centisome position: 8.23

GC content: 72.49

Gene sequence:

>978_bases
ATGGACAAGCTCAAGGCCTTCGAGTCGTTCGTGTCGGTGGCGACGCGCGGCAGCCTCACCGCCGCGGCGCGCGCCGAGGG
CGTGGCGCCTGCCATCATGGGGCGGCGCCTGGATGCCCTGGAGGAGCATCTGGGCGTGAAGCTCATGGTGCGCACCACGC
GGCGCATCAGTCTCACGCACGAGGGCAGCGCCTTCCTGGAGGATTGCCAGCGCCTGCTGGCCGACGTGGCAGAGGCAGAG
GCCAGCGTGTCGGTCGGCGGGGCCCGGGCCACCGGGCATCTGCGCATCACCGCGCCCGCGGGGTTCGGGCGGCGCCACGT
GGCGCCGCTGGTGCCGCTGTTCCGCGACCTGCATCCCGACGTGCGCATTTCCCTCAACCTCAGCGACCGCGTGGTGGACC
TGGCGGGGGAGGGGTTCGATTGCGCCGTGCGCGTGGGCGACCTGCCCGATTCCTCGCTGGTGAGCGTGCGCATCGCCGAC
AACCGCAGGCTCTGCGTGGCCACGCCCGCCTACCTGGAGCGCCACGGCACGCCGAAGCACCCGTCCGAACTCGCGCGGCA
CGCCTGCCTGATGCTGTCGAGCGACGCGTCGCAGACGCGCGGCTGGGCCTTCCGCATGCCCGCTGCGGACCCGGGCGGCG
CGCCCGGCGACGTGGTGCATGTCAAGCCCACCGGCCCCCTCGATTGTTCCGACGGGCAGGTGCTGCACGACTGGTGCCTG
GGGGGCTGGGGCATCGCGTGGCGCAGCACCTGGGAGGTGGAGGCCGAGATCGCCGCCGGCCGGCTGGTGGCCGTGCTGGA
GGATTTCGCCGCGCCGCCCAACGGTATCTACGTGGTCTTTCCCCATGCCCGCCACCTGCCGCTGCGCGTGCGGCTGTGGA
TCGACTACCTCAAGCACCACTATGCGCAGCCGGCGTTCTGGCGCCGCGCCGCGCATGCGCTCACCGCTCCCGTCCTCCCG
TCTCCTCCTTCGCCATGA

Upstream 100 bases:

>100_bases
AACCCGTGACTTTTTTGCATGAATCCGCGCAGGGTGGCCGGCTCCTTGGCGTGCAACATCCGTGTGTCCGGCGGTTACAG
GCTGCCCATAATTGCAGCCC

Downstream 100 bases:

>100_bases
CACACCGACTCCATGCCCCGCTTCCCGATGTTTTGCGCCGCCCCGCTCCGGGCCGGGTGTCCGCCGCCCTGGCGCTGGCC
GGCCTGATGGCCGGCTGCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MDKLKAFESFVSVATRGSLTAAARAEGVAPAIMGRRLDALEEHLGVKLMVRTTRRISLTHEGSAFLEDCQRLLADVAEAE
ASVSVGGARATGHLRITAPAGFGRRHVAPLVPLFRDLHPDVRISLNLSDRVVDLAGEGFDCAVRVGDLPDSSLVSVRIAD
NRRLCVATPAYLERHGTPKHPSELARHACLMLSSDASQTRGWAFRMPAADPGGAPGDVVHVKPTGPLDCSDGQVLHDWCL
GGWGIAWRSTWEVEAEIAAGRLVAVLEDFAAPPNGIYVVFPHARHLPLRVRLWIDYLKHHYAQPAFWRRAAHALTAPVLP
SPPSP

Sequences:

>Translated_325_residues
MDKLKAFESFVSVATRGSLTAAARAEGVAPAIMGRRLDALEEHLGVKLMVRTTRRISLTHEGSAFLEDCQRLLADVAEAE
ASVSVGGARATGHLRITAPAGFGRRHVAPLVPLFRDLHPDVRISLNLSDRVVDLAGEGFDCAVRVGDLPDSSLVSVRIAD
NRRLCVATPAYLERHGTPKHPSELARHACLMLSSDASQTRGWAFRMPAADPGGAPGDVVHVKPTGPLDCSDGQVLHDWCL
GGWGIAWRSTWEVEAEIAAGRLVAVLEDFAAPPNGIYVVFPHARHLPLRVRLWIDYLKHHYAQPAFWRRAAHALTAPVLP
SPPSP
>Mature_325_residues
MDKLKAFESFVSVATRGSLTAAARAEGVAPAIMGRRLDALEEHLGVKLMVRTTRRISLTHEGSAFLEDCQRLLADVAEAE
ASVSVGGARATGHLRITAPAGFGRRHVAPLVPLFRDLHPDVRISLNLSDRVVDLAGEGFDCAVRVGDLPDSSLVSVRIAD
NRRLCVATPAYLERHGTPKHPSELARHACLMLSSDASQTRGWAFRMPAADPGGAPGDVVHVKPTGPLDCSDGQVLHDWCL
GGWGIAWRSTWEVEAEIAAGRLVAVLEDFAAPPNGIYVVFPHARHLPLRVRLWIDYLKHHYAQPAFWRRAAHALTAPVLP
SPPSP

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=305, Percent_Identity=34.4262295081967, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=33.9041095890411, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1789440, Length=295, Percent_Identity=29.4915254237288, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI145693193, Length=297, Percent_Identity=29.96632996633, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1787128, Length=297, Percent_Identity=26.9360269360269, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789639, Length=303, Percent_Identity=28.7128712871287, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1787589, Length=301, Percent_Identity=28.9036544850498, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789173, Length=276, Percent_Identity=26.8115942028986, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=24.8275862068966, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788706, Length=174, Percent_Identity=25.8620689655172, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790011, Length=320, Percent_Identity=23.4375, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35192; Mature: 35192

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLKAFESFVSVATRGSLTAAARAEGVAPAIMGRRLDALEEHLGVKLMVRTTRRISLTH
CCHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEECEEEEEEE
EGSAFLEDCQRLLADVAEAEASVSVGGARATGHLRITAPAGFGRRHVAPLVPLFRDLHPD
CCHHHHHHHHHHHHHHHHHHCCEEECCCEEECEEEEEECCCCCCCHHHHHHHHHHHCCCC
VRISLNLSDRVVDLAGEGFDCAVRVGDLPDSSLVSVRIADNRRLCVATPAYLERHGTPKH
EEEEEECCCEEEEECCCCCEEEEEECCCCCCCEEEEEEECCCEEEEECHHHHHHCCCCCC
PSELARHACLMLSSDASQTRGWAFRMPAADPGGAPGDVVHVKPTGPLDCSDGQVLHDWCL
HHHHHHHHHEEECCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCEEHHHHC
GGWGIAWRSTWEVEAEIAAGRLVAVLEDFAAPPNGIYVVFPHARHLPLRVRLWIDYLKHH
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHHHHHHHHH
YAQPAFWRRAAHALTAPVLPSPPSP
CCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDKLKAFESFVSVATRGSLTAAARAEGVAPAIMGRRLDALEEHLGVKLMVRTTRRISLTH
CCHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEECEEEEEEE
EGSAFLEDCQRLLADVAEAEASVSVGGARATGHLRITAPAGFGRRHVAPLVPLFRDLHPD
CCHHHHHHHHHHHHHHHHHHCCEEECCCEEECEEEEEECCCCCCCHHHHHHHHHHHCCCC
VRISLNLSDRVVDLAGEGFDCAVRVGDLPDSSLVSVRIADNRRLCVATPAYLERHGTPKH
EEEEEECCCEEEEECCCCCEEEEEECCCCCCCEEEEEEECCCEEEEECHHHHHHCCCCCC
PSELARHACLMLSSDASQTRGWAFRMPAADPGGAPGDVVHVKPTGPLDCSDGQVLHDWCL
HHHHHHHHHEEECCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCEEHHHHC
GGWGIAWRSTWEVEAEIAAGRLVAVLEDFAAPPNGIYVVFPHARHLPLRVRLWIDYLKHH
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHHHHHHHHH
YAQPAFWRRAAHALTAPVLPSPPSP
CCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]