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The map label for this gene is nrfG [H]

Identifier: 120597411

GI number: 120597411

Start: 636863

End: 637543

Strand: Direct

Name: nrfG [H]

Synonym: Sputw3181_0580

Alternate gene names: 120597411

Gene position: 636863-637543 (Clockwise)

Preceding gene: 120597410

Following gene: 120597412

Centisome position: 13.53

GC content: 46.84

Gene sequence:

>681_bases
ATGGAGACTGGAATGGTGAGCTTAGGACTAGGCATTGGCATCAGTTTACTGGTGTTTGTTGCTTACATTTACTGGCAGCA
CATGCGTCTAAACCTAGTAATGGGAATAGCACAGTTGCCAAACATGTCGAGCAAAGTGCCCTTTGCGATGGGAATCGTCC
TAGTGCTGCTTACCCTTGCTATCTACAGTCAAACAGGCCGTTTTAGGGATTGGGATCAAGGTCGGCTTGATGAAAATATT
GACTATTTAGTGGCGGCCGAAATCACCAAAGCCAGTCGTTGGGCTCAGAGTGAGCCACAAAATACCTTGGCACTTATGGC
ATTAGCTCAGGCATACAGTGCGGGTGGTTTGTACTCAGATGCGGTGCGGACCTTGGATAAATTACTCGAAATAGGGGGAG
AAGATGCCGAAGTGTTAGGGGCAAAAGCTAACGCGCTTTATTACCGCGAGCTTCGGCAAATGACACCTGAAACCCAAGAG
GTGATTGCCAAAGCATTAGCACTCTCTCCCTTTGAGCCTCAAACCCATATGTTACTCGCGGCAGATGCTTACTTACATGC
TCGTAACCAAGAGGCCATTGACCATTGGCAGCTTTTGCTCAAACACAATGGGGACAGTATCAACCGTGAAGCAATCAATA
ATGCGATTGCAAAAGCACAAAGCAAAATGAATGAAAGATGA

Upstream 100 bases:

>100_bases
AATATTGAGAAACCCAGCGAAATGAAAGAGATGAAAAATAAAAAGATGATGCCTGCACACGGTTAATCAGGCTCCTAAAA
CGAGTTGTTTGGAGAGTGAA

Downstream 100 bases:

>100_bases
AACCCGTTTCTATCAACCCTCGCCGTAACGATCTGTCCCTGCAAGGCATCTGGCGAGGTGTTGATATTTTAATAAAAGGA
TAAATAGGATGAAGAAATAC

Product: formate-dependent nitrite reductase, NrfG protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
METGMVSLGLGIGISLLVFVAYIYWQHMRLNLVMGIAQLPNMSSKVPFAMGIVLVLLTLAIYSQTGRFRDWDQGRLDENI
DYLVAAEITKASRWAQSEPQNTLALMALAQAYSAGGLYSDAVRTLDKLLEIGGEDAEVLGAKANALYYRELRQMTPETQE
VIAKALALSPFEPQTHMLLAADAYLHARNQEAIDHWQLLLKHNGDSINREAINNAIAKAQSKMNER

Sequences:

>Translated_226_residues
METGMVSLGLGIGISLLVFVAYIYWQHMRLNLVMGIAQLPNMSSKVPFAMGIVLVLLTLAIYSQTGRFRDWDQGRLDENI
DYLVAAEITKASRWAQSEPQNTLALMALAQAYSAGGLYSDAVRTLDKLLEIGGEDAEVLGAKANALYYRELRQMTPETQE
VIAKALALSPFEPQTHMLLAADAYLHARNQEAIDHWQLLLKHNGDSINREAINNAIAKAQSKMNER
>Mature_226_residues
METGMVSLGLGIGISLLVFVAYIYWQHMRLNLVMGIAQLPNMSSKVPFAMGIVLVLLTLAIYSQTGRFRDWDQGRLDENI
DYLVAAEITKASRWAQSEPQNTLALMALAQAYSAGGLYSDAVRTLDKLLEIGGEDAEVLGAKANALYYRELRQMTPETQE
VIAKALALSPFEPQTHMLLAADAYLHARNQEAIDHWQLLLKHNGDSINREAINNAIAKAQSKMNER

Specific function: Required for formate-dependent nitrite reduction. Not required for the biosynthesis of any of the c-type cytochromes nor for the secretion of the periplasmic cytochromes [H]

COG id: COG4235

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI1790513, Length=124, Percent_Identity=36.2903225806452, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013026
- InterPro:   IPR011990
- InterPro:   IPR019734 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25092; Mature: 25092

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50293 TPR_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METGMVSLGLGIGISLLVFVAYIYWQHMRLNLVMGIAQLPNMSSKVPFAMGIVLVLLTLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
IYSQTGRFRDWDQGRLDENIDYLVAAEITKASRWAQSEPQNTLALMALAQAYSAGGLYSD
HHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHH
AVRTLDKLLEIGGEDAEVLGAKANALYYRELRQMTPETQEVIAKALALSPFEPQTHMLLA
HHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHEEEE
ADAYLHARNQEAIDHWQLLLKHNGDSINREAINNAIAKAQSKMNER
HHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METGMVSLGLGIGISLLVFVAYIYWQHMRLNLVMGIAQLPNMSSKVPFAMGIVLVLLTLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
IYSQTGRFRDWDQGRLDENIDYLVAAEITKASRWAQSEPQNTLALMALAQAYSAGGLYSD
HHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHH
AVRTLDKLLEIGGEDAEVLGAKANALYYRELRQMTPETQEVIAKALALSPFEPQTHMLLA
HHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHEEEE
ADAYLHARNQEAIDHWQLLLKHNGDSINREAINNAIAKAQSKMNER
HHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]