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The map label for this gene is fliA

Identifier: 120597321

GI number: 120597321

Start: 530129

End: 530845

Strand: Direct

Name: fliA

Synonym: Sputw3181_0490

Alternate gene names: 120597321

Gene position: 530129-530845 (Clockwise)

Preceding gene: 120597320

Following gene: 120597322

Centisome position: 11.26

GC content: 43.1

Gene sequence:

>717_bases
ATGACGACAGATGGCCAACTTGCATATCAACAAACCTGTGATTATCCAATCGAAGAACGGCAATCTGAACAACAGGTTAT
TCGGCAATATTTACCACTGATTAAACGTATCGTGTCGCAGATGCGTAGCCACTGTGGCGTCATGTTATCGCTTGAGGATA
TGGAACAAATAGGGATGATGGCCTTGCTTGAGTCATCGCGTCGCTATCCTGGAGAGCTCGATAACGGATTTATTTCTTTC
GCGGGACAACGTATCCGCGGCGCAATATTGGATGAGTTAAGAAGGCAAGATTGGCGTCCTCGCCCTGTTAGGCAACAAGC
CCATGAACTGAATGATGTTGTTAGACGCTTGAGCCGTGAAATGGGACGGGAACCTAGCGATCTCGAGGTTGCCAGTGCAT
TAGGTCTTAGTGCTGAAGATTACCGAGAACGCTTATATTCCACACAAGCGGAGTCGATGAAAAGCTTAGATGAATTGTTG
TTGGTGGGCGAACATCCGGTCGATAGAGGCAATGATGTTGAACAATTGATGCTAAAGCAGACCTTGATCAAAGTTATTTC
AAAATTAAATGAAAGAGAACAGTTAATCCTGTCGCTTTACTATCATCATGAGCTGAATTTAAAAGAAATTGCCATGGCAT
TAGGGTTAACAGAAACACGTATTTGTCAGTTACATAAATCAGCAATTTCACAGTTAAAAACAATATTGCAGCGCTAA

Upstream 100 bases:

>100_bases
AGCCCTTATTTTGATGGTGAAAGTGCTATCTGATAACAAATTGCCATCAGATATTGATGAAGTACTTTTTACCCGTATGG
TGATCCAGTAAGGGGCCGAT

Downstream 100 bases:

>100_bases
AGTTAGAAGTGAGAAGATCGTAATGAGTAAACTAGTTGGGCTGTTAATTATTTTAGCTTCAGTATTTGGTGGATATGCTT
GGGCTGGTGGTGCGTTGGCT

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-F factor [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTTDGQLAYQQTCDYPIEERQSEQQVIRQYLPLIKRIVSQMRSHCGVMLSLEDMEQIGMMALLESSRRYPGELDNGFISF
AGQRIRGAILDELRRQDWRPRPVRQQAHELNDVVRRLSREMGREPSDLEVASALGLSAEDYRERLYSTQAESMKSLDELL
LVGEHPVDRGNDVEQLMLKQTLIKVISKLNEREQLILSLYYHHELNLKEIAMALGLTETRICQLHKSAISQLKTILQR

Sequences:

>Translated_238_residues
MTTDGQLAYQQTCDYPIEERQSEQQVIRQYLPLIKRIVSQMRSHCGVMLSLEDMEQIGMMALLESSRRYPGELDNGFISF
AGQRIRGAILDELRRQDWRPRPVRQQAHELNDVVRRLSREMGREPSDLEVASALGLSAEDYRERLYSTQAESMKSLDELL
LVGEHPVDRGNDVEQLMLKQTLIKVISKLNEREQLILSLYYHHELNLKEIAMALGLTETRICQLHKSAISQLKTILQR
>Mature_237_residues
TTDGQLAYQQTCDYPIEERQSEQQVIRQYLPLIKRIVSQMRSHCGVMLSLEDMEQIGMMALLESSRRYPGELDNGFISFA
GQRIRGAILDELRRQDWRPRPVRQQAHELNDVVRRLSREMGREPSDLEVASALGLSAEDYRERLYSTQAESMKSLDELLL
VGEHPVDRGNDVEQLMLKQTLIKVISKLNEREQLILSLYYHHELNLKEIAMALGLTETRICQLHKSAISQLKTILQR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=234, Percent_Identity=37.1794871794872, Blast_Score=147, Evalue=4e-37,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27579; Mature: 27448

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDGQLAYQQTCDYPIEERQSEQQVIRQYLPLIKRIVSQMRSHCGVMLSLEDMEQIGMM
CCCCCCHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHH
ALLESSRRYPGELDNGFISFAGQRIRGAILDELRRQDWRPRPVRQQAHELNDVVRRLSRE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
MGREPSDLEVASALGLSAEDYRERLYSTQAESMKSLDELLLVGEHPVDRGNDVEQLMLKQ
HCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TLIKVISKLNEREQLILSLYYHHELNLKEIAMALGLTETRICQLHKSAISQLKTILQR
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTDGQLAYQQTCDYPIEERQSEQQVIRQYLPLIKRIVSQMRSHCGVMLSLEDMEQIGMM
CCCCCHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHH
ALLESSRRYPGELDNGFISFAGQRIRGAILDELRRQDWRPRPVRQQAHELNDVVRRLSRE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
MGREPSDLEVASALGLSAEDYRERLYSTQAESMKSLDELLLVGEHPVDRGNDVEQLMLKQ
HCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TLIKVISKLNEREQLILSLYYHHELNLKEIAMALGLTETRICQLHKSAISQLKTILQR
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501040; 12620739 [H]