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The map label for this gene is prfB [H]

Identifier: 119947032

GI number: 119947032

Start: 4239405

End: 4240394

Strand: Reverse

Name: prfB [H]

Synonym: Ping_3429

Alternate gene names: 119947032

Gene position: 4240394-4239405 (Counterclockwise)

Preceding gene: 119947040

Following gene: 119947031

Centisome position: 93.0

GC content: 44.95

Gene sequence:

>990_bases
GTGTGTCGTGAATTAGAATCACCAGAAGTATGGAGTGAACCGGAACGCGCGCAGGCATTAGGTAAAGAGCGCAGTGCCTT
AGAGTTGGTTGTTGACACTATAGATGCGTTAAGTGCGGGAACCGAAGATATTCTAATGCTGGTTGAGATGGCCACAGAAG
AAAACGATCAGGACAGTTTTGATGAAGCCCAAAAAGAAGTAAATGAGCTGGAAAAAAAATTAGAGGTGTTAGAATTTCGC
CGTATGTTCTCCGGTAATCACGATGCTTGCTCCTGCTATTTAGATATTCAATCGGGCTCTGGCGGAACAGAAGCTCAGGA
CTGGGCAAATATACTCCTGCGTATGTACCTGCGTTGGGGAGAAGCACACGGTTATAAAACCGAGTTGATGGAAGTCTCCG
CAGGGGATGTTGCCGGTATTAAAGGAGCCACCATTCGTTTTACTGGCGAATATGCTTTTGGCTGGTTACGCACCGAAACC
GGTGTTCACCGCTTGGTTCGAAAATCACCCTTTGATTCATCAGGGCGCCGTCATACCTCCTTTGCATCTGCCTTTATCTA
CCCTGAAATTGAAGATGATATTGATATTCAGATTAACCCGGCGGATTTACGCATTGATGTGTACCGTGCATCGGGCGCGG
GCGGGCAACACGTCAATACAACGGAATCTGCGGTACGTATTACCCACGTACCCACCAATATTGTGGTGCAGTGTCAAAAT
GACCGCTCACAACATAAGAATAAAGATCAGGCAATGAAGCAGCTAAAAGCCAAATTGTATGAACATGAATTGCAAAAGCA
AAATGCCGAAAAACAAATCAGTGAAGACAGTAAATCAGATATTGGCTGGGGCAGTCAAATACGTTCATATGTTTTAGATG
ATGCACGTATCAAAGATTTAAGAACCGGTATTGAGAGTCGTAATACGCAAGCGGTATTGGATGGTGGCTTGGATAAATTT
ATCGAAGCCAGTTTGAAGTCAGGACTGTAG

Upstream 100 bases:

>100_bases
GTAAATCCTATTTTAAATAAAGTAAAAGATTTAACTGAGCGCGCACAAGTACTTCGGGGGTATCTTTGACTACGACGCCA
AAGCTGAACGTTTAGAAGAA

Downstream 100 bases:

>100_bases
CTTTGAGTCGCGAGCCGCTAGCTCGAAGCTCATAGTTCGAAGCTCGTAGCCTTCCAGCTTTTTATGCCGTGGGGTCGAGA
ATCAGGTCAACAAACCAAAT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MCRELESPEVWSEPERAQALGKERSALELVVDTIDALSAGTEDILMLVEMATEENDQDSFDEAQKEVNELEKKLEVLEFR
RMFSGNHDACSCYLDIQSGSGGTEAQDWANILLRMYLRWGEAHGYKTELMEVSAGDVAGIKGATIRFTGEYAFGWLRTET
GVHRLVRKSPFDSSGRRHTSFASAFIYPEIEDDIDIQINPADLRIDVYRASGAGGQHVNTTESAVRITHVPTNIVVQCQN
DRSQHKNKDQAMKQLKAKLYEHELQKQNAEKQISEDSKSDIGWGSQIRSYVLDDARIKDLRTGIESRNTQAVLDGGLDKF
IEASLKSGL

Sequences:

>Translated_329_residues
MCRELESPEVWSEPERAQALGKERSALELVVDTIDALSAGTEDILMLVEMATEENDQDSFDEAQKEVNELEKKLEVLEFR
RMFSGNHDACSCYLDIQSGSGGTEAQDWANILLRMYLRWGEAHGYKTELMEVSAGDVAGIKGATIRFTGEYAFGWLRTET
GVHRLVRKSPFDSSGRRHTSFASAFIYPEIEDDIDIQINPADLRIDVYRASGAGGQHVNTTESAVRITHVPTNIVVQCQN
DRSQHKNKDQAMKQLKAKLYEHELQKQNAEKQISEDSKSDIGWGSQIRSYVLDDARIKDLRTGIESRNTQAVLDGGLDKF
IEASLKSGL
>Mature_329_residues
MCRELESPEVWSEPERAQALGKERSALELVVDTIDALSAGTEDILMLVEMATEENDQDSFDEAQKEVNELEKKLEVLEFR
RMFSGNHDACSCYLDIQSGSGGTEAQDWANILLRMYLRWGEAHGYKTELMEVSAGDVAGIKGATIRFTGEYAFGWLRTET
GVHRLVRKSPFDSSGRRHTSFASAFIYPEIEDDIDIQINPADLRIDVYRASGAGGQHVNTTESAVRITHVPTNIVVQCQN
DRSQHKNKDQAMKQLKAKLYEHELQKQNAEKQISEDSKSDIGWGSQIRSYVLDDARIKDLRTGIESRNTQAVLDGGLDKF
IEASLKSGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=267, Percent_Identity=35.9550561797753, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI34577120, Length=321, Percent_Identity=30.5295950155763, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI166795305, Length=170, Percent_Identity=37.6470588235294, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI2367172, Length=326, Percent_Identity=75.4601226993865, Blast_Score=533, Evalue=1e-153,
Organism=Escherichia coli, GI1787462, Length=240, Percent_Identity=40.8333333333333, Blast_Score=188, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=260, Percent_Identity=32.6923076923077, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321295, Length=321, Percent_Identity=33.3333333333333, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI19921226, Length=236, Percent_Identity=38.9830508474576, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 36892; Mature: 36892

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRELESPEVWSEPERAQALGKERSALELVVDTIDALSAGTEDILMLVEMATEENDQDSF
CCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHH
DEAQKEVNELEKKLEVLEFRRMFSGNHDACSCYLDIQSGSGGTEAQDWANILLRMYLRWG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
EAHGYKTELMEVSAGDVAGIKGATIRFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTS
CCCCCHHHHEEECCCCCCCCCCCEEEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCCHH
FASAFIYPEIEDDIDIQINPADLRIDVYRASGAGGQHVNTTESAVRITHVPTNIVVQCQN
HHHEEECCCCCCCCEEEECCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCEEEEEECC
DRSQHKNKDQAMKQLKAKLYEHELQKQNAEKQISEDSKSDIGWGSQIRSYVLDDARIKDL
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHH
RTGIESRNTQAVLDGGLDKFIEASLKSGL
HHHHHCCCCHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MCRELESPEVWSEPERAQALGKERSALELVVDTIDALSAGTEDILMLVEMATEENDQDSF
CCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHH
DEAQKEVNELEKKLEVLEFRRMFSGNHDACSCYLDIQSGSGGTEAQDWANILLRMYLRWG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
EAHGYKTELMEVSAGDVAGIKGATIRFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTS
CCCCCHHHHEEECCCCCCCCCCCEEEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCCHH
FASAFIYPEIEDDIDIQINPADLRIDVYRASGAGGQHVNTTESAVRITHVPTNIVVQCQN
HHHEEECCCCCCCCEEEECCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCEEEEEECC
DRSQHKNKDQAMKQLKAKLYEHELQKQNAEKQISEDSKSDIGWGSQIRSYVLDDARIKDL
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHH
RTGIESRNTQAVLDGGLDKFIEASLKSGL
HHHHHCCCCHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA