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The map label for this gene is tuf

Identifier: 119947040

GI number: 119947040

Start: 4249454

End: 4250638

Strand: Reverse

Name: tuf

Synonym: Ping_3437

Alternate gene names: 119947040

Gene position: 4250638-4249454 (Counterclockwise)

Preceding gene: 119947041

Following gene: 119947032

Centisome position: 93.22

GC content: 43.54

Gene sequence:

>1185_bases
GTGTCTAAAGCAAAATTTGAACGTAAAAAACCACATCTAAACGTTGGTACAATCGGCCACGTCGATCATGGTAAAACAAC
GCTAACAGCTGCTATCTCTGCTGTTTTAACTAAAGCACACGGCGGTGAAGTTAAAGATTTCGCATCTATCGATAACGCAC
CTGAAGAGCGCGAACGTGGTATTACAATCAATACCTCTCACATCGAGTACGATACTGATACGCGTCATTATGCACACGTA
GACTGTCCTGGACACGCCGATTACGTTAAAAACATGATCACTGGTGCTGCACAAATGGATGCTGGTATCCTTGTTGTTGC
TGCAACTGATGGTCCTATGCCACAAACGCGTGAGCACATCCTACTTTCTCGTCAAGTTGGTGTTCCTCACTTAATCGTGT
TCATGAACAAATGTGACATGGTTGATGATGAAGAGCTACTTGAACTAGTAGAAATGGAAGTTCGTGAACTTCTTTCTGAA
TATGACTTCCCAGGTGATGACTTACCAGTTATCCAGGGTTCTGCTCTTAAAGCACTTGAAGGCGACCCAGTATGGGAAGC
TAAAGTTATGGAACTTGCTGACGCTCTTGATACTTATATCCCGCTTCCTGAGCGTGATATCGACAAACCATTCATCCTAC
CAATTGAAGATGTATTCTCAATTGCTGGTCGTGGTACTGTTGTTACTGGTCGTGTTGAGCGCGGTATCATCAAAGTTGGT
CAAGAAGTTGAAATCATTGGTCTACGTCCAACAGTTAAAACTACTTGTACTGGTGTTGAGATGTTCCGTAAACTGCTTGA
CGAAGGTCGTGCTGGTGAGAACGTTGGTATCCTTTTACGTGGTACTAAACGTGATGACGTTGAACGTGGTCAAGTTCTTG
CTAAACCTGGTTCAATCAAGCCACACACTAAATTTGAAGGCGAAGTGTACATCCTAAGTAAAGATGAGGGTGGTCGTCAC
ACTCCTTTCTTTAAAGGTTACCGTCCACAGTTCTTCTTCCGTACAACAGACATCACAGGTGCTGTTGAGCTTCCTGAAGG
TGTTGAAATGGTAATGCCTGGTGATAACCTTAAGTTTGTTGTTGACTTAATTGGTCCAGTTGCTATGGATGAAGGTCTAC
GTTTTGCTATCCGTGAAGGTGGCCGTACTGTAGGTGCTGGTGTTGTTTCTAAAATCATCGAGTAA

Upstream 100 bases:

>100_bases
CTAACGCAATTATTGAAGCGAAATAAAAAATAAATTAAACTATCATCTTTCCATGTGATGGCATGTGGAGAGGTATTAAC
TGATAAAGAAGGTACACACT

Downstream 100 bases:

>100_bases
TTTTTACTCCTTAGATTGAACAATGCAAGAAAGAGAGCTTAGGCTCTCTTTTTTTATGCGTGAAGGAAAATGAGAAAGCT
GTCAGTCTTCTGCTTTCAGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSKAKFERKKPHLNVGTIGHVDHGKTTLTAAISAVLTKAHGGEVKDFASIDNAPEERERGITINTSHIEYDTDTRHYAHV
DCPGHADYVKNMITGAAQMDAGILVVAATDGPMPQTREHILLSRQVGVPHLIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGDPVWEAKVMELADALDTYIPLPERDIDKPFILPIEDVFSIAGRGTVVTGRVERGIIKVG
QEVEIIGLRPTVKTTCTGVEMFRKLLDEGRAGENVGILLRGTKRDDVERGQVLAKPGSIKPHTKFEGEVYILSKDEGGRH
TPFFKGYRPQFFFRTTDITGAVELPEGVEMVMPGDNLKFVVDLIGPVAMDEGLRFAIREGGRTVGAGVVSKIIE

Sequences:

>Translated_394_residues
MSKAKFERKKPHLNVGTIGHVDHGKTTLTAAISAVLTKAHGGEVKDFASIDNAPEERERGITINTSHIEYDTDTRHYAHV
DCPGHADYVKNMITGAAQMDAGILVVAATDGPMPQTREHILLSRQVGVPHLIVFMNKCDMVDDEELLELVEMEVRELLSE
YDFPGDDLPVIQGSALKALEGDPVWEAKVMELADALDTYIPLPERDIDKPFILPIEDVFSIAGRGTVVTGRVERGIIKVG
QEVEIIGLRPTVKTTCTGVEMFRKLLDEGRAGENVGILLRGTKRDDVERGQVLAKPGSIKPHTKFEGEVYILSKDEGGRH
TPFFKGYRPQFFFRTTDITGAVELPEGVEMVMPGDNLKFVVDLIGPVAMDEGLRFAIREGGRTVGAGVVSKIIE
>Mature_393_residues
SKAKFERKKPHLNVGTIGHVDHGKTTLTAAISAVLTKAHGGEVKDFASIDNAPEERERGITINTSHIEYDTDTRHYAHVD
CPGHADYVKNMITGAAQMDAGILVVAATDGPMPQTREHILLSRQVGVPHLIVFMNKCDMVDDEELLELVEMEVRELLSEY
DFPGDDLPVIQGSALKALEGDPVWEAKVMELADALDTYIPLPERDIDKPFILPIEDVFSIAGRGTVVTGRVERGIIKVGQ
EVEIIGLRPTVKTTCTGVEMFRKLLDEGRAGENVGILLRGTKRDDVERGQVLAKPGSIKPHTKFEGEVYILSKDEGGRHT
PFFKGYRPQFFFRTTDITGAVELPEGVEMVMPGDNLKFVVDLIGPVAMDEGLRFAIREGGRTVGAGVVSKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.8680203045685, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=29.6629213483146, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=29.6629213483146, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4503475, Length=450, Percent_Identity=29.1111111111111, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4503471, Length=457, Percent_Identity=29.9781181619256, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI194018522, Length=328, Percent_Identity=30.4878048780488, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI194097354, Length=328, Percent_Identity=30.4878048780488, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI194018520, Length=328, Percent_Identity=30.4878048780488, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI46094014, Length=329, Percent_Identity=31.0030395136778, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI54607086, Length=348, Percent_Identity=28.1609195402299, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4503507, Length=369, Percent_Identity=26.8292682926829, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI53729339, Length=229, Percent_Identity=31.0043668122271, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI53729337, Length=229, Percent_Identity=31.0043668122271, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=82.9516539440204, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=82.9516539440204, Blast_Score=656, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=380, Percent_Identity=29.2105263157895, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789108, Length=322, Percent_Identity=30.7453416149068, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI48994988, Length=237, Percent_Identity=32.0675105485232, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=50.8771929824561, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25141371, Length=385, Percent_Identity=44.1558441558442, Blast_Score=330, Evalue=9e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=30.9050772626932, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=30.9050772626932, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=28.8018433179723, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=434, Percent_Identity=27.8801843317972, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=27.8801843317972, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=341, Percent_Identity=27.5659824046921, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=29.0969899665552, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17509919, Length=441, Percent_Identity=28.7981859410431, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=35.0649350649351, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=27.6595744680851, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=63.0653266331658, Blast_Score=525, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=351, Percent_Identity=33.6182336182336, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=351, Percent_Identity=33.6182336182336, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=340, Percent_Identity=30.5882352941176, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322937, Length=464, Percent_Identity=22.6293103448276, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=290, Percent_Identity=28.9655172413793, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=27.3109243697479, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=56.4885496183206, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=56.4885496183206, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=50.259067357513, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=28.8888888888889, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=28.8888888888889, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=28.8888888888889, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=28.8888888888889, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24652838, Length=451, Percent_Identity=29.2682926829268, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI17137572, Length=451, Percent_Identity=29.2682926829268, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=29.9342105263158, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45550900, Length=337, Percent_Identity=29.080118694362, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137380, Length=336, Percent_Identity=31.8452380952381, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24647046, Length=377, Percent_Identity=27.5862068965517, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24647048, Length=377, Percent_Identity=27.5862068965517, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28572034, Length=175, Percent_Identity=33.7142857142857, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_PSYIN (A1T056)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944720.1
- ProteinModelPortal:   A1T056
- SMR:   A1T056
- STRING:   A1T056
- GeneID:   4625591
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3437
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   ERKPHIN
- PhylomeDB:   A1T056
- ProtClustDB:   PRK00049
- BioCyc:   PING357804:PING_3437-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43263; Mature: 43132

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAKFERKKPHLNVGTIGHVDHGKTTLTAAISAVLTKAHGGEVKDFASIDNAPEERERG
CCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHCC
ITINTSHIEYDTDTRHYAHVDCPGHADYVKNMITGAAQMDAGILVVAATDGPMPQTREHI
EEEEECEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHCCCCEEEEEECCCCCCCCHHHE
LLSRQVGVPHLIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
GDPVWEAKVMELADALDTYIPLPERDIDKPFILPIEDVFSIAGRGTVVTGRVERGIIKVG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCEEEECHHHHHHHHCC
QEVEIIGLRPTVKTTCTGVEMFRKLLDEGRAGENVGILLRGTKRDDVERGQVLAKPGSIK
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCC
PHTKFEGEVYILSKDEGGRHTPFFKGYRPQFFFRTTDITGAVELPEGVEMVMPGDNLKFV
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCEEH
VDLIGPVAMDEGLRFAIREGGRTVGAGVVSKIIE
EEHHCCHHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
SKAKFERKKPHLNVGTIGHVDHGKTTLTAAISAVLTKAHGGEVKDFASIDNAPEERERG
CCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHCC
ITINTSHIEYDTDTRHYAHVDCPGHADYVKNMITGAAQMDAGILVVAATDGPMPQTREHI
EEEEECEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHCCCCEEEEEECCCCCCCCHHHE
LLSRQVGVPHLIVFMNKCDMVDDEELLELVEMEVRELLSEYDFPGDDLPVIQGSALKALE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
GDPVWEAKVMELADALDTYIPLPERDIDKPFILPIEDVFSIAGRGTVVTGRVERGIIKVG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCEEEECHHHHHHHHCC
QEVEIIGLRPTVKTTCTGVEMFRKLLDEGRAGENVGILLRGTKRDDVERGQVLAKPGSIK
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCC
PHTKFEGEVYILSKDEGGRHTPFFKGYRPQFFFRTTDITGAVELPEGVEMVMPGDNLKFV
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCEEH
VDLIGPVAMDEGLRFAIREGGRTVGAGVVSKIIE
EEHHCCHHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA