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The map label for this gene is purF [H]

Identifier: 119945652

GI number: 119945652

Start: 2390773

End: 2392287

Strand: Reverse

Name: purF [H]

Synonym: Ping_1965

Alternate gene names: 119945652

Gene position: 2392287-2390773 (Counterclockwise)

Preceding gene: 119945653

Following gene: 119945651

Centisome position: 52.47

GC content: 41.78

Gene sequence:

>1515_bases
ATGTGTGGAATCGTTGGTATAGTTGGAGCAACTCCCGTTAATCAAAGTATCTATGATGCATTGACCGTACTACAACACCG
TGGGCAGGATGCTGCCGGCATCGTTACTATTTCAAATGATAATTATAAACAGCGTAAAGCCAATGGTATGGTGAAAGATG
TTTTTGATTCTAAGCATATGCAGCGTTTAAAAGGTAATATTGGTATAGGTCATGTACGCTATCCGACTGCGGGCAGTTCC
AGTGCCGCAGAAGCTCAACCGTTTTATGTCAACTCCCCCTGGGGAATTTCCCTCGCACATAACGGGAATTTAACCAACGC
CCAAAATTTACGAGATTCATTAGTTAAGTCCCGCCGTCATATCAATACAACGTCTGATTCTGAAGTGCTGTTAAATGTTT
TTGCTGCCGAGCTTGATAAAGTTGAAAGTGATAAATTATCACCAGAAGATGTGTTTACAGCTGTCGGAGAGCTTCATAAA
AGCGTTAAAGGTGCGTACGCTGTTGTATCATTGATCATCGGCCACGGCATGGTCGCATTTCGCGACCCTTATGGTATCCG
CCCATTAGTATTAGGCTCTCGTAAGACAGAAGAAGGGAATTTAGAATATATTGTGGCTTCAGAAAGTGTGGGTCTTGATA
CTATTGGTTTTGAAGTTATCCGTGATGTTGAACCTGGTGAAGCAGTTTATATCACCGAAGATCGGCAGTTATTTACCCAA
CAATGTGCTGAAAACCCGCGCTTAACACCTTGTATTTTTGAATATGTTTATTTAGCGCGTCCCGATTCCACCTTAAGCGG
TATTTCTGTTTATGAAGCGCGTTTAGAAATGGGCCGTAAATTAGGCGCAAAAATAAAACGAGAATGGGCTGATGTAAAGA
TTGATGTTGTGATACCTATCCCAGAAACATCGAATGATATTGCGCAGGAAATATCTTTGGAAATGGGATTGCCTTATCGT
CAGGGTTTTGTAAAAAATCGTTATATTGGCAGAACATTTATTATGCCGGGGCAAACACAGCGCCGCAATGCCGTGCGCCG
TAAATTAAATCCAATTACATCTGAGTTTAAAGATAAAGCGGTTTTATTAGTGGATGACTCGATAGTACGTGGTACTACCT
CGGAACAGATTGTCGAAATGGTGCGGGATGCCGGTGCTAAAAAAGTGTATTTTGCTTCAGCCGCGCCACAGGTTTGCCAT
CCAAATGTCTACGGCATTGATATGCCAACAGCCAGTGAATTGATTGCTTATAACCGCACGGTAGAGCAGATTAGCGATTG
TATCGGGACTGACGCGCTTATTTACCAGGACTTAGAAGATCTCAAGGATGCGCTTCGTAAACAAAACCCTAATACAGATG
ATTTCGAGTGTTCAGTCTTTGATGGACTCTATGTGACAGGTGACATAGACAAAAACTACTTCGATTATGTTGAAAGTTTA
CGCAATGAAGATGCGAAGATAATTCAAGAGCAGAAAGAAGAGATAGCAAGTTTAGAGATGTATAACGAATCTTAA

Upstream 100 bases:

>100_bases
ACACCCATTTAAAATTTACAATCACTATCATTTATATAAAATAACCTGTTACTTGGTATGACTGATATAACTTATTTTTC
TAATAATTAAGGCATTATTT

Downstream 100 bases:

>100_bases
ATTTATTTTTATTCTGTTAAAAAACCACAAATTTGTTTGTGGTTTTTTTGCCCAGAAACCCGAACAGCAAAAAGTCAGTC
AATTTTCCCCTCTCAATTTT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MCGIVGIVGATPVNQSIYDALTVLQHRGQDAAGIVTISNDNYKQRKANGMVKDVFDSKHMQRLKGNIGIGHVRYPTAGSS
SAAEAQPFYVNSPWGISLAHNGNLTNAQNLRDSLVKSRRHINTTSDSEVLLNVFAAELDKVESDKLSPEDVFTAVGELHK
SVKGAYAVVSLIIGHGMVAFRDPYGIRPLVLGSRKTEEGNLEYIVASESVGLDTIGFEVIRDVEPGEAVYITEDRQLFTQ
QCAENPRLTPCIFEYVYLARPDSTLSGISVYEARLEMGRKLGAKIKREWADVKIDVVIPIPETSNDIAQEISLEMGLPYR
QGFVKNRYIGRTFIMPGQTQRRNAVRRKLNPITSEFKDKAVLLVDDSIVRGTTSEQIVEMVRDAGAKKVYFASAAPQVCH
PNVYGIDMPTASELIAYNRTVEQISDCIGTDALIYQDLEDLKDALRKQNPNTDDFECSVFDGLYVTGDIDKNYFDYVESL
RNEDAKIIQEQKEEIASLEMYNES

Sequences:

>Translated_504_residues
MCGIVGIVGATPVNQSIYDALTVLQHRGQDAAGIVTISNDNYKQRKANGMVKDVFDSKHMQRLKGNIGIGHVRYPTAGSS
SAAEAQPFYVNSPWGISLAHNGNLTNAQNLRDSLVKSRRHINTTSDSEVLLNVFAAELDKVESDKLSPEDVFTAVGELHK
SVKGAYAVVSLIIGHGMVAFRDPYGIRPLVLGSRKTEEGNLEYIVASESVGLDTIGFEVIRDVEPGEAVYITEDRQLFTQ
QCAENPRLTPCIFEYVYLARPDSTLSGISVYEARLEMGRKLGAKIKREWADVKIDVVIPIPETSNDIAQEISLEMGLPYR
QGFVKNRYIGRTFIMPGQTQRRNAVRRKLNPITSEFKDKAVLLVDDSIVRGTTSEQIVEMVRDAGAKKVYFASAAPQVCH
PNVYGIDMPTASELIAYNRTVEQISDCIGTDALIYQDLEDLKDALRKQNPNTDDFECSVFDGLYVTGDIDKNYFDYVESL
RNEDAKIIQEQKEEIASLEMYNES
>Mature_504_residues
MCGIVGIVGATPVNQSIYDALTVLQHRGQDAAGIVTISNDNYKQRKANGMVKDVFDSKHMQRLKGNIGIGHVRYPTAGSS
SAAEAQPFYVNSPWGISLAHNGNLTNAQNLRDSLVKSRRHINTTSDSEVLLNVFAAELDKVESDKLSPEDVFTAVGELHK
SVKGAYAVVSLIIGHGMVAFRDPYGIRPLVLGSRKTEEGNLEYIVASESVGLDTIGFEVIRDVEPGEAVYITEDRQLFTQ
QCAENPRLTPCIFEYVYLARPDSTLSGISVYEARLEMGRKLGAKIKREWADVKIDVVIPIPETSNDIAQEISLEMGLPYR
QGFVKNRYIGRTFIMPGQTQRRNAVRRKLNPITSEFKDKAVLLVDDSIVRGTTSEQIVEMVRDAGAKKVYFASAAPQVCH
PNVYGIDMPTASELIAYNRTVEQISDCIGTDALIYQDLEDLKDALRKQNPNTDDFECSVFDGLYVTGDIDKNYFDYVESL
RNEDAKIIQEQKEEIASLEMYNES

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=472, Percent_Identity=35.8050847457627, Blast_Score=263, Evalue=3e-70,
Organism=Escherichia coli, GI1788651, Length=505, Percent_Identity=67.7227722772277, Blast_Score=724, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=246, Percent_Identity=27.2357723577236, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=463, Percent_Identity=33.4773218142549, Blast_Score=238, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6323958, Length=511, Percent_Identity=50.4892367906067, Blast_Score=479, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24659598, Length=485, Percent_Identity=36.4948453608247, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24659604, Length=478, Percent_Identity=36.6108786610879, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI28573187, Length=475, Percent_Identity=35.1578947368421, Blast_Score=268, Evalue=9e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55999; Mature: 55999

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGATPVNQSIYDALTVLQHRGQDAAGIVTISNDNYKQRKANGMVKDVFDSKHM
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCHHHHHHHHHHH
QRLKGNIGIGHVRYPTAGSSSAAEAQPFYVNSPWGISLAHNGNLTNAQNLRDSLVKSRRH
HHHHCCCCCCEEECCCCCCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHC
INTTSDSEVLLNVFAAELDKVESDKLSPEDVFTAVGELHKSVKGAYAVVSLIIGHGMVAF
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
RDPYGIRPLVLGSRKTEEGNLEYIVASESVGLDTIGFEVIRDVEPGEAVYITEDRQLFTQ
CCCCCCCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECHHHHHHH
QCAENPRLTPCIFEYVYLARPDSTLSGISVYEARLEMGRKLGAKIKREWADVKIDVVIPI
HHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEC
PETSNDIAQEISLEMGLPYRQGFVKNRYIGRTFIMPGQTQRRNAVRRKLNPITSEFKDKA
CCCCHHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCE
VLLVDDSIVRGTTSEQIVEMVRDAGAKKVYFASAAPQVCHPNVYGIDMPTASELIAYNRT
EEEEECCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHH
VEQISDCIGTDALIYQDLEDLKDALRKQNPNTDDFECSVFDGLYVTGDIDKNYFDYVESL
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCHHHHHHHHH
RNEDAKIIQEQKEEIASLEMYNES
HCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCGIVGIVGATPVNQSIYDALTVLQHRGQDAAGIVTISNDNYKQRKANGMVKDVFDSKHM
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCHHHHHHHHHHH
QRLKGNIGIGHVRYPTAGSSSAAEAQPFYVNSPWGISLAHNGNLTNAQNLRDSLVKSRRH
HHHHCCCCCCEEECCCCCCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHC
INTTSDSEVLLNVFAAELDKVESDKLSPEDVFTAVGELHKSVKGAYAVVSLIIGHGMVAF
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
RDPYGIRPLVLGSRKTEEGNLEYIVASESVGLDTIGFEVIRDVEPGEAVYITEDRQLFTQ
CCCCCCCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECHHHHHHH
QCAENPRLTPCIFEYVYLARPDSTLSGISVYEARLEMGRKLGAKIKREWADVKIDVVIPI
HHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEC
PETSNDIAQEISLEMGLPYRQGFVKNRYIGRTFIMPGQTQRRNAVRRKLNPITSEFKDKA
CCCCHHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCE
VLLVDDSIVRGTTSEQIVEMVRDAGAKKVYFASAAPQVCHPNVYGIDMPTASELIAYNRT
EEEEECCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHH
VEQISDCIGTDALIYQDLEDLKDALRKQNPNTDDFECSVFDGLYVTGDIDKNYFDYVESL
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCHHHHHHHHH
RNEDAKIIQEQKEEIASLEMYNES
HCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]