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The map label for this gene is phnE [H]

Identifier: 119945470

GI number: 119945470

Start: 2149071

End: 2150561

Strand: Reverse

Name: phnE [H]

Synonym: Ping_1768

Alternate gene names: 119945470

Gene position: 2150561-2149071 (Counterclockwise)

Preceding gene: 119945471

Following gene: 119945465

Centisome position: 47.17

GC content: 41.31

Gene sequence:

>1491_bases
ATGCCTAAAATCATCACCGTTAGTGGATTCTCTTTTGTAGGACACTGGCAAAAAATAACGCTATTGCTTTTACTGATGGT
ATCAGTATGTTGGTTTTTTGCTGATACCGAAGTGATTGCGCTCGAACCTTGGATTGAACTTGGACGTATGGGACTGGGTT
TTATTCATCCCGATTTTTTCGCGACAGAGTATTTACTTGAAGCTCTGTGGCAAACCATCAGTTTTGCTCTACTTGGGATA
ACGCTCGGATTGCTATTGGGATTTCCATTAGCATTGTGGTATCAGCATCCTTTGATTGCGGCAATCTGTGCGTTTATTCG
TGCTATCCATGAGATATTTTGGGCGCTAATATTTTTACAAATATTCGGTTTATCGGCCATTACGGGTATTTTAGCTATTG
CCCTTCCTTATGCCGCCACGTTTGCCCGAATATTTTCAGATATTTTACAGCAAGCACCAAAACACACATTATTTTCTCTG
CCAAAGGGCACAGATAAACTTTCGGCATTTATTTATGGCCGCTGGATGCATATGTATCCACAGATTGTCGATTATATTCG
TTACCGTTTCGAGTGTGCGCTGCGCAGTAGTGCTGTGCTCGGTTTTATTGGTATGCCTACCTTAGGTTTTTATTTAGAAT
CGGCGTTTCGCCAAGGGCATTATGAACAAGGTGGTGCATTATTAATCCTATTCGTGCTGTTAATTGGCACTATACGCTTT
TGGGCTAAACCGATTTTTTTGTGGTTGTATTTACCACTCGCCGTCTATTTTTTACCGCCTATTCCGGCCATTGACGGTCA
ACTTATTTGGCGCTTTATCAGTGTTGATATTTTACCACCTATGCTGCAGGGGCAACCCTTCTTAAGCTGGTTTAATCCTG
ATATTTTGCCGAAGGTCTTTGACTGGGCTTATAACTTAATCAGCCAGCAGGTTTTTCCCGGAATGATTGCAACCTTAGTC
TTGGCTTTTTGTGCATTAGGATTAACCTATTTGCTGACCCTGGTTTTATTACCTTTTAATACCCGGCTGATGATGCCAAA
AGTTGTCATATTAATCATGCGGGCCGTGAATTTAATATTACGTTCACTGCCAGAATATTTATTGGCCTTTGTGCTTATGA
TGCTATTAGGGCCTTCCATGTTACCCGCTATTATCGCTTTAGCATTGCACAATAGTGGCCTTATGGTCTTTTTAATGGCG
CGTCAGGCTGATAGCGTGACAGTGCCACTGACTTATAAGCCCAGGATTGATCAATACAGTTATCTGGTTCTTCCTGCCCT
ATACCCCCATTTTATGGGCCTATTGTTTTATCGTTTTGAAGTAATTATCCGTGAAACAGCTATTTTGGGCATGATTGGGG
TAATGACGTTAGGATTTTATGTTGACAGTAATTTTGCTGAAATTCGCTTTAGCGCAGCCTTATTATTATTGGTTTTCACT
GCAGGGCTCAATGTCATCGTTGATTATCTCGCTCGGCGATTATTGCATTAA

Upstream 100 bases:

>100_bases
GGCAGATACTGTCAAAAATGCAGTGATATGAAGATATTAATACTAAAAAACAACTTTTTTATAACCCACTTTTCCCTATT
CAGTCACCACCCTTTCTATT

Downstream 100 bases:

>100_bases
CCGCGTGATTATTTACAAGCTTGCTAGTGTTAATTTTCCTGCGCAATGCAAGATCTCATCTTTAATACATTGGTTATTAG
TGCTGTTTTTGCTGCCTTTT

Product: ABC transporter complex for phosphonate inner membrane binding subunit

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MPKIITVSGFSFVGHWQKITLLLLLMVSVCWFFADTEVIALEPWIELGRMGLGFIHPDFFATEYLLEALWQTISFALLGI
TLGLLLGFPLALWYQHPLIAAICAFIRAIHEIFWALIFLQIFGLSAITGILAIALPYAATFARIFSDILQQAPKHTLFSL
PKGTDKLSAFIYGRWMHMYPQIVDYIRYRFECALRSSAVLGFIGMPTLGFYLESAFRQGHYEQGGALLILFVLLIGTIRF
WAKPIFLWLYLPLAVYFLPPIPAIDGQLIWRFISVDILPPMLQGQPFLSWFNPDILPKVFDWAYNLISQQVFPGMIATLV
LAFCALGLTYLLTLVLLPFNTRLMMPKVVILIMRAVNLILRSLPEYLLAFVLMMLLGPSMLPAIIALALHNSGLMVFLMA
RQADSVTVPLTYKPRIDQYSYLVLPALYPHFMGLLFYRFEVIIRETAILGMIGVMTLGFYVDSNFAEIRFSAALLLLVFT
AGLNVIVDYLARRLLH

Sequences:

>Translated_496_residues
MPKIITVSGFSFVGHWQKITLLLLLMVSVCWFFADTEVIALEPWIELGRMGLGFIHPDFFATEYLLEALWQTISFALLGI
TLGLLLGFPLALWYQHPLIAAICAFIRAIHEIFWALIFLQIFGLSAITGILAIALPYAATFARIFSDILQQAPKHTLFSL
PKGTDKLSAFIYGRWMHMYPQIVDYIRYRFECALRSSAVLGFIGMPTLGFYLESAFRQGHYEQGGALLILFVLLIGTIRF
WAKPIFLWLYLPLAVYFLPPIPAIDGQLIWRFISVDILPPMLQGQPFLSWFNPDILPKVFDWAYNLISQQVFPGMIATLV
LAFCALGLTYLLTLVLLPFNTRLMMPKVVILIMRAVNLILRSLPEYLLAFVLMMLLGPSMLPAIIALALHNSGLMVFLMA
RQADSVTVPLTYKPRIDQYSYLVLPALYPHFMGLLFYRFEVIIRETAILGMIGVMTLGFYVDSNFAEIRFSAALLLLVFT
AGLNVIVDYLARRLLH
>Mature_495_residues
PKIITVSGFSFVGHWQKITLLLLLMVSVCWFFADTEVIALEPWIELGRMGLGFIHPDFFATEYLLEALWQTISFALLGIT
LGLLLGFPLALWYQHPLIAAICAFIRAIHEIFWALIFLQIFGLSAITGILAIALPYAATFARIFSDILQQAPKHTLFSLP
KGTDKLSAFIYGRWMHMYPQIVDYIRYRFECALRSSAVLGFIGMPTLGFYLESAFRQGHYEQGGALLILFVLLIGTIRFW
AKPIFLWLYLPLAVYFLPPIPAIDGQLIWRFISVDILPPMLQGQPFLSWFNPDILPKVFDWAYNLISQQVFPGMIATLVL
AFCALGLTYLLTLVLLPFNTRLMMPKVVILIMRAVNLILRSLPEYLLAFVLMMLLGPSMLPAIIALALHNSGLMVFLMAR
QADSVTVPLTYKPRIDQYSYLVLPALYPHFMGLLFYRFEVIIRETAILGMIGVMTLGFYVDSNFAEIRFSAALLLLVFTA
GLNVIVDYLARRLLH

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 56275; Mature: 56144

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKIITVSGFSFVGHWQKITLLLLLMVSVCWFFADTEVIALEPWIELGRMGLGFIHPDFF
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCCCHHH
ATEYLLEALWQTISFALLGITLGLLLGFPLALWYQHPLIAAICAFIRAIHEIFWALIFLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
IFGLSAITGILAIALPYAATFARIFSDILQQAPKHTLFSLPKGTDKLSAFIYGRWMHMYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHH
QIVDYIRYRFECALRSSAVLGFIGMPTLGFYLESAFRQGHYEQGGALLILFVLLIGTIRF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
WAKPIFLWLYLPLAVYFLPPIPAIDGQLIWRFISVDILPPMLQGQPFLSWFNPDILPKVF
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHH
DWAYNLISQQVFPGMIATLVLAFCALGLTYLLTLVLLPFNTRLMMPKVVILIMRAVNLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RSLPEYLLAFVLMMLLGPSMLPAIIALALHNSGLMVFLMARQADSVTVPLTYKPRIDQYS
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCH
YLVLPALYPHFMGLLFYRFEVIIRETAILGMIGVMTLGFYVDSNFAEIRFSAALLLLVFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AGLNVIVDYLARRLLH
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKIITVSGFSFVGHWQKITLLLLLMVSVCWFFADTEVIALEPWIELGRMGLGFIHPDFF
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCCCHHH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HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA