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The map label for this gene is rfbI [H]

Identifier: 119944538

GI number: 119944538

Start: 971535

End: 972500

Strand: Direct

Name: rfbI [H]

Synonym: Ping_0772

Alternate gene names: 119944538

Gene position: 971535-972500 (Clockwise)

Preceding gene: 119944537

Following gene: 119944539

Centisome position: 21.31

GC content: 36.75

Gene sequence:

>966_bases
ATGGCCTACAGAATCGAAATTCAACCATCAGGTGTTCACTTTCAAAGTGAAAATAATCTTTTAGATGATGCGCTAGACCA
GTCAATCCCATTAGAGCATAGTTGCAAAACAGGAGAATGTGGCACCTGTTCGGCAGAAGTGATTTTTGGTGATATTGAAA
ATGAGAATGATGAAATAGTTAGTCAAGGGGCTATTCTGACTTGTCAATCTAGAGCATTATCTGACGCCATATTAAAAGCT
AAATATTATCCTGAGCTTGCATCACAACAAATACAAACATTACCCAGCAAAGTAGCAAGCTTTGATTATGTAACTGCTGA
CATTATAGTTATTAAACTGAGATTTCCACCGACAGCTAAATTTGATTTTCTGCCTGGACAATATGTTGATTTGAATTTTA
AAGGCATCAAGCGCAGTTATTCTATTGCTAATGCCAAACAAGTTTCAGACGGTATTGAGTTGCATATTCGTAAGGTAGCA
GAAGGTAAAATGTCAGAGGCTGTATTCTCTGGTTTAAAAGAAGGTTTACTGATGCGATTAGAAGGTCCTAAGGGAACTTT
TTTTGTGCGCGAATCAAATAAGCCGATTATTTTCCTCGCAGGAGGAACGGGTATTGCTCCTGTTAAAGCAATGATCGAAG
ATCTTGTTGCTAATGAAAGTAAGCGTGAAATTCATATTTATTGGGGAATGAATAATCCAAGTGCTTTCTATTTAGATAAG
TTACAGCAGTTTGCTGAAGAAAATTCAAACATTTATTACACTCCCGTTTTATCTGGTGAGGAGCAATGGGATGGGCGAAT
GGGATTTGTACATCAGGCTGTATGTGATGATTTTGAGTCTCTAAATGAATATCAAGTTTATGCTTGCGGTTCACCATTGA
TGATTAATGCCGCCAAAATATCTTTTATTGAAAAGCAGTTATCAAAAGAACAGTTTTTCTCCGATGCATTTACACCTGCA
AAATAA

Upstream 100 bases:

>100_bases
ATCATTAGGTTGGGAATATTCAGTTTCTTTGGAAGATGGTTTGACGTTGGCCTACCAGTGGTTTGTTGATAATCAAGATA
AGTTCAGAGGTTAGATAAAC

Downstream 100 bases:

>100_bases
TTTTAATTTCTATTTGGAGTATCCATGAAAGCAGTCATTTTAGCCGGTGGTTTAGGCACTCGTCTCAGCGAAGAAACCTC
CTTAAAGCCAAAGCCTATGG

Product: CDP-6-deoxy-delta-3,4-glucoseen reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MAYRIEIQPSGVHFQSENNLLDDALDQSIPLEHSCKTGECGTCSAEVIFGDIENENDEIVSQGAILTCQSRALSDAILKA
KYYPELASQQIQTLPSKVASFDYVTADIIVIKLRFPPTAKFDFLPGQYVDLNFKGIKRSYSIANAKQVSDGIELHIRKVA
EGKMSEAVFSGLKEGLLMRLEGPKGTFFVRESNKPIIFLAGGTGIAPVKAMIEDLVANESKREIHIYWGMNNPSAFYLDK
LQQFAEENSNIYYTPVLSGEEQWDGRMGFVHQAVCDDFESLNEYQVYACGSPLMINAAKISFIEKQLSKEQFFSDAFTPA
K

Sequences:

>Translated_321_residues
MAYRIEIQPSGVHFQSENNLLDDALDQSIPLEHSCKTGECGTCSAEVIFGDIENENDEIVSQGAILTCQSRALSDAILKA
KYYPELASQQIQTLPSKVASFDYVTADIIVIKLRFPPTAKFDFLPGQYVDLNFKGIKRSYSIANAKQVSDGIELHIRKVA
EGKMSEAVFSGLKEGLLMRLEGPKGTFFVRESNKPIIFLAGGTGIAPVKAMIEDLVANESKREIHIYWGMNNPSAFYLDK
LQQFAEENSNIYYTPVLSGEEQWDGRMGFVHQAVCDDFESLNEYQVYACGSPLMINAAKISFIEKQLSKEQFFSDAFTPA
K
>Mature_320_residues
AYRIEIQPSGVHFQSENNLLDDALDQSIPLEHSCKTGECGTCSAEVIFGDIENENDEIVSQGAILTCQSRALSDAILKAK
YYPELASQQIQTLPSKVASFDYVTADIIVIKLRFPPTAKFDFLPGQYVDLNFKGIKRSYSIANAKQVSDGIELHIRKVAE
GKMSEAVFSGLKEGLLMRLEGPKGTFFVRESNKPIIFLAGGTGIAPVKAMIEDLVANESKREIHIYWGMNNPSAFYLDKL
QQFAEENSNIYYTPVLSGEEQWDGRMGFVHQAVCDDFESLNEYQVYACGSPLMINAAKISFIEKQLSKEQFFSDAFTPAK

Specific function: Catalyzes The Reduction Of Soluble Flavins By Reduced Pyridine Nucleotides. Seems To Reduces The Complexed Fe(3+) Iron Of Siderophores To Fe(2+), Thus Releasing It From The Chelator. [C]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=232, Percent_Identity=29.7413793103448, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 35763; Mature: 35632

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYRIEIQPSGVHFQSENNLLDDALDQSIPLEHSCKTGECGTCSAEVIFGDIENENDEIV
CEEEEEECCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHH
SQGAILTCQSRALSDAILKAKYYPELASQQIQTLPSKVASFDYVTADIIVIKLRFPPTAK
HCCEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCC
FDFLPGQYVDLNFKGIKRSYSIANAKQVSDGIELHIRKVAEGKMSEAVFSGLKEGLLMRL
EECCCCCEEEEEHHCCCCHHCCCCHHHHCCCHHEEEEHHHCCHHHHHHHHHHHCCEEEEE
EGPKGTFFVRESNKPIIFLAGGTGIAPVKAMIEDLVANESKREIHIYWGMNNPSAFYLDK
ECCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
LQQFAEENSNIYYTPVLSGEEQWDGRMGFVHQAVCDDFESLNEYQVYACGSPLMINAAKI
HHHHHHCCCCEEEEEEECCCHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEHHHH
SFIEKQLSKEQFFSDAFTPAK
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AYRIEIQPSGVHFQSENNLLDDALDQSIPLEHSCKTGECGTCSAEVIFGDIENENDEIV
EEEEEECCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHH
SQGAILTCQSRALSDAILKAKYYPELASQQIQTLPSKVASFDYVTADIIVIKLRFPPTAK
HCCEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCC
FDFLPGQYVDLNFKGIKRSYSIANAKQVSDGIELHIRKVAEGKMSEAVFSGLKEGLLMRL
EECCCCCEEEEEHHCCCCHHCCCCHHHHCCCHHEEEEHHHCCHHHHHHHHHHHCCEEEEE
EGPKGTFFVRESNKPIIFLAGGTGIAPVKAMIEDLVANESKREIHIYWGMNNPSAFYLDK
ECCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
LQQFAEENSNIYYTPVLSGEEQWDGRMGFVHQAVCDDFESLNEYQVYACGSPLMINAAKI
HHHHHHCCCCEEEEEEECCCHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEHHHH
SFIEKQLSKEQFFSDAFTPAK
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]