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The map label for this gene is fcl [H]

Identifier: 119944537

GI number: 119944537

Start: 970560

End: 971528

Strand: Direct

Name: fcl [H]

Synonym: Ping_0771

Alternate gene names: 119944537

Gene position: 970560-971528 (Clockwise)

Preceding gene: 119944536

Following gene: 119944538

Centisome position: 21.29

GC content: 39.53

Gene sequence:

>969_bases
ATGAATAATAAAAAACGTATTTTTGTTGCGGGGCATAATGGCATGGTTGGCTCTGCTATTGTCTGTCAGCTAGAAAACAA
CGAAAATATTGAGATTGTAGTACGCTCACGCAAAGAATTGGATCTAACCAATCAGCAAGCTGTCTCTGATTTTTTTCAAA
CCGAAAAAATAGATCAAGTTTATCTGGCTGCGGCTAAAGTAGGGGGGATTGTTGCCAACAATACTTACCCTGCTGATTTC
ATTTACGAAAACTTAATAATTGAATGTAATATTATTAATAGCGCCCATTTAGCCGGTATTCAGCGGTTGTTATTCTTAGG
CTCTTCCTGTATTTATCCGAAATTGGCGGAGCAGCCTATGTCTGAATCAGCGTTATTAACGGGAACATTAGAAGAAACCA
ATGAGCCTTATGCTATTGCAAAAATAGCAGGCATCAAATTGTGTGAAAGTTATAATCGCCAGTATGGACGAGATTATCGC
AGTGTTATGCCAACCAATCTTTATGGTGTACGTGATAACTTCCATCCTGAAAACAGTCATGTTATTCCAGCGTTGCTGCG
CCGTTTTCATGAAGCTAAGCTGAATGGTGATAAAGAAGTGATTGCATGGGGCAGTGGCAAACCGATGCGGGAGTTTTTAT
ATGTTGATGATATGGCAGCAGCCTCTATTTATGTTATGAATTTAGGCAAAGAACTCTATAACTCCAATACAGAGCCTATG
TTGAGTCATATTAATGTTGGCACGGGTGTTGATTGCACGATCAAAGACTTAGTTGAAACGGTCGCTAAGGTTGTTGGATT
TGAAGGTGAAATCAAATTTGATGCAACGAAACCAGATGGGGCGCCACGCAAATTGATGAATGTAGAAAGATTGGAATCAT
TAGGTTGGGAATATTCAGTTTCTTTGGAAGATGGTTTGACGTTGGCCTACCAGTGGTTTGTTGATAATCAAGATAAGTTC
AGAGGTTAG

Upstream 100 bases:

>100_bases
AAAGCATTCAGATATTATCTTAACTTTAAATCTTTCCTCTGTGTAGCGAAGCGCCTCAGGGACCTCTGTGGTAAATAGAT
TTTAAGATTAAAAGGTATTT

Downstream 100 bases:

>100_bases
ATAAACATGGCCTACAGAATCGAAATTCAACCATCAGGTGTTCACTTTCAAAGTGAAAATAATCTTTTAGATGATGCGCT
AGACCAGTCAATCCCATTAG

Product: bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase and GDP-4-dehydro-6-L-deoxygalactose reductase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNNKKRIFVAGHNGMVGSAIVCQLENNENIEIVVRSRKELDLTNQQAVSDFFQTEKIDQVYLAAAKVGGIVANNTYPADF
IYENLIIECNIINSAHLAGIQRLLFLGSSCIYPKLAEQPMSESALLTGTLEETNEPYAIAKIAGIKLCESYNRQYGRDYR
SVMPTNLYGVRDNFHPENSHVIPALLRRFHEAKLNGDKEVIAWGSGKPMREFLYVDDMAAASIYVMNLGKELYNSNTEPM
LSHINVGTGVDCTIKDLVETVAKVVGFEGEIKFDATKPDGAPRKLMNVERLESLGWEYSVSLEDGLTLAYQWFVDNQDKF
RG

Sequences:

>Translated_322_residues
MNNKKRIFVAGHNGMVGSAIVCQLENNENIEIVVRSRKELDLTNQQAVSDFFQTEKIDQVYLAAAKVGGIVANNTYPADF
IYENLIIECNIINSAHLAGIQRLLFLGSSCIYPKLAEQPMSESALLTGTLEETNEPYAIAKIAGIKLCESYNRQYGRDYR
SVMPTNLYGVRDNFHPENSHVIPALLRRFHEAKLNGDKEVIAWGSGKPMREFLYVDDMAAASIYVMNLGKELYNSNTEPM
LSHINVGTGVDCTIKDLVETVAKVVGFEGEIKFDATKPDGAPRKLMNVERLESLGWEYSVSLEDGLTLAYQWFVDNQDKF
RG
>Mature_322_residues
MNNKKRIFVAGHNGMVGSAIVCQLENNENIEIVVRSRKELDLTNQQAVSDFFQTEKIDQVYLAAAKVGGIVANNTYPADF
IYENLIIECNIINSAHLAGIQRLLFLGSSCIYPKLAEQPMSESALLTGTLEETNEPYAIAKIAGIKLCESYNRQYGRDYR
SVMPTNLYGVRDNFHPENSHVIPALLRRFHEAKLNGDKEVIAWGSGKPMREFLYVDDMAAASIYVMNLGKELYNSNTEPM
LSHINVGTGVDCTIKDLVETVAKVVGFEGEIKFDATKPDGAPRKLMNVERLESLGWEYSVSLEDGLTLAYQWFVDNQDKF
RG

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=320, Percent_Identity=30.9375, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI7657641, Length=339, Percent_Identity=24.188790560472, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI42516563, Length=332, Percent_Identity=24.6987951807229, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788365, Length=320, Percent_Identity=70.9375, Blast_Score=500, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17554428, Length=321, Percent_Identity=30.8411214953271, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI115532424, Length=341, Percent_Identity=25.2199413489736, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922778, Length=326, Percent_Identity=31.9018404907975, Blast_Score=167, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 36048; Mature: 36048

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKKRIFVAGHNGMVGSAIVCQLENNENIEIVVRSRKELDLTNQQAVSDFFQTEKIDQV
CCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
YLAAAKVGGIVANNTYPADFIYENLIIECNIINSAHLAGIQRLLFLGSSCIYPKLAEQPM
HHHHHHHCCEEECCCCCHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHCCCC
SESALLTGTLEETNEPYAIAKIAGIKLCESYNRQYGRDYRSVMPTNLYGVRDNFHPENSH
CCCEEEEEEHHHCCCCEEEEHHHHHHHHHHHHHHHCCHHHHCCCCCEEECCCCCCCCCCC
VIPALLRRFHEAKLNGDKEVIAWGSGKPMREFLYVDDMAAASIYVMNLGKELYNSNTEPM
CHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHEECCCHHHHHHHEEHHCHHHHCCCCCHH
LSHINVGTGVDCTIKDLVETVAKVVGFEGEIKFDATKPDGAPRKLMNVERLESLGWEYSV
HHHCCCCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEE
SLEDGLTLAYQWFVDNQDKFRG
ECCCCCEEEEEEEECCCHHCCC
>Mature Secondary Structure
MNNKKRIFVAGHNGMVGSAIVCQLENNENIEIVVRSRKELDLTNQQAVSDFFQTEKIDQV
CCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
YLAAAKVGGIVANNTYPADFIYENLIIECNIINSAHLAGIQRLLFLGSSCIYPKLAEQPM
HHHHHHHCCEEECCCCCHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHCCCC
SESALLTGTLEETNEPYAIAKIAGIKLCESYNRQYGRDYRSVMPTNLYGVRDNFHPENSH
CCCEEEEEEHHHCCCCEEEEHHHHHHHHHHHHHHHCCHHHHCCCCCEEECCCCCCCCCCC
VIPALLRRFHEAKLNGDKEVIAWGSGKPMREFLYVDDMAAASIYVMNLGKELYNSNTEPM
CHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHEECCCHHHHHHHEEHHCHHHHCCCCCHH
LSHINVGTGVDCTIKDLVETVAKVVGFEGEIKFDATKPDGAPRKLMNVERLESLGWEYSV
HHHCCCCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEE
SLEDGLTLAYQWFVDNQDKFRG
ECCCCCEEEEEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]