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The map label for this gene is rfbF [H]

Identifier: 119944539

GI number: 119944539

Start: 972525

End: 973298

Strand: Direct

Name: rfbF [H]

Synonym: Ping_0773

Alternate gene names: 119944539

Gene position: 972525-973298 (Clockwise)

Preceding gene: 119944538

Following gene: 119944540

Centisome position: 21.33

GC content: 40.44

Gene sequence:

>774_bases
ATGAAAGCAGTCATTTTAGCCGGTGGTTTAGGCACTCGTCTCAGCGAAGAAACCTCCTTAAAGCCAAAGCCTATGGTTGA
AATTGGTGGTAAGCCCATTTTATGGCACATTATGAAACAATATTCAGCTCATGGTATCAATGATTTTATTATTTGCTGTG
GTTATAAAGGTTATGTCATCAAAGAATATTTTGCTAACTATTTCCTACATATGTCCGATGTTACTTTTGATATGAAAAAT
AATGAGATGAAAGTGCACTGTGAGCGGGCAGAACCTTGGACTGTGACATTGATTGATACAGGGGATGCTTCTATGACTGG
TGGTCGCTTAGCTCGAGTCGCAGATCACATAAAAGACGAAGAAGCTTTTTGTTTTACCTATGGCGATGGTGTAAGTGATG
TTGATATCACAAAAACTATCGAATTCCATAAATCGCACGGCAAAAAAGCAACTCTTACAGCTACATTTCCACCGGGGCGT
TTTGGTGCGTTAGATATTACTGCGGGTAAAGTGAATAGCTTCAAGGAAAAGCCGAGAGGTGATGGAGCACTGATTAATGG
TGGATTTTTTGTGCTTTCCCCTGAAGTATTAAAACTCATCGATGGGGATAGCTGCACATGGGAACAAGAGCCATTAAAAA
CATTAGCACAAGAAAATGAGTTAATGTCTTATGAGCATCAAGGTTTCTGGCAGCCGATGGATACACTGCGTGATAAAGTG
CATCTTGAAGAGTTATGGCAAGCAGGCAATGCACCTTGGAAAATTTGGGAGTAA

Upstream 100 bases:

>100_bases
CGCCAAAATATCTTTTATTGAAAAGCAGTTATCAAAAGAACAGTTTTTCTCCGATGCATTTACACCTGCAAAATAATTTT
AATTTCTATTTGGAGTATCC

Downstream 100 bases:

>100_bases
ATGATGAATCCTAGTTTTTGGAAAGATAAAAGAGTATTTATTACAGGGCATACTGGTTTTAAAGGAAGCTGGTTGTCATT
ATGGCTTCAGGAGATGGGCG

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKAVILAGGLGTRLSEETSLKPKPMVEIGGKPILWHIMKQYSAHGINDFIICCGYKGYVIKEYFANYFLHMSDVTFDMKN
NEMKVHCERAEPWTVTLIDTGDASMTGGRLARVADHIKDEEAFCFTYGDGVSDVDITKTIEFHKSHGKKATLTATFPPGR
FGALDITAGKVNSFKEKPRGDGALINGGFFVLSPEVLKLIDGDSCTWEQEPLKTLAQENELMSYEHQGFWQPMDTLRDKV
HLEELWQAGNAPWKIWE

Sequences:

>Translated_257_residues
MKAVILAGGLGTRLSEETSLKPKPMVEIGGKPILWHIMKQYSAHGINDFIICCGYKGYVIKEYFANYFLHMSDVTFDMKN
NEMKVHCERAEPWTVTLIDTGDASMTGGRLARVADHIKDEEAFCFTYGDGVSDVDITKTIEFHKSHGKKATLTATFPPGR
FGALDITAGKVNSFKEKPRGDGALINGGFFVLSPEVLKLIDGDSCTWEQEPLKTLAQENELMSYEHQGFWQPMDTLRDKV
HLEELWQAGNAPWKIWE
>Mature_257_residues
MKAVILAGGLGTRLSEETSLKPKPMVEIGGKPILWHIMKQYSAHGINDFIICCGYKGYVIKEYFANYFLHMSDVTFDMKN
NEMKVHCERAEPWTVTLIDTGDASMTGGRLARVADHIKDEEAFCFTYGDGVSDVDITKTIEFHKSHGKKATLTATFPPGR
FGALDITAGKVNSFKEKPRGDGALINGGFFVLSPEVLKLIDGDSCTWEQEPLKTLAQENELMSYEHQGFWQPMDTLRDKV
HLEELWQAGNAPWKIWE

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=246, Percent_Identity=29.6747967479675, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI11761619, Length=246, Percent_Identity=29.6747967479675, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI133931050, Length=248, Percent_Identity=30.241935483871, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6320148, Length=252, Percent_Identity=31.3492063492063, Blast_Score=112, Evalue=6e-26,
Organism=Drosophila melanogaster, GI21355443, Length=246, Percent_Identity=29.2682926829268, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644084, Length=246, Percent_Identity=29.2682926829268, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 28883; Mature: 28883

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVILAGGLGTRLSEETSLKPKPMVEIGGKPILWHIMKQYSAHGINDFIICCGYKGYVI
CCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCHH
KEYFANYFLHMSDVTFDMKNNEMKVHCERAEPWTVTLIDTGDASMTGGRLARVADHIKDE
HHHHHHHEEEECCEEEEECCCEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHCCCC
EAFCFTYGDGVSDVDITKTIEFHKSHGKKATLTATFPPGRFGALDITAGKVNSFKEKPRG
CEEEEEECCCCCCCEEHEEEEEHHCCCCEEEEEEECCCCCCCEEEEECCCCHHHHCCCCC
DGALINGGFFVLSPEVLKLIDGDSCTWEQEPLKTLAQENELMSYEHQGFWQPMDTLRDKV
CCEEEECCEEEECHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
HLEELWQAGNAPWKIWE
HHHHHHHCCCCCEEECC
>Mature Secondary Structure
MKAVILAGGLGTRLSEETSLKPKPMVEIGGKPILWHIMKQYSAHGINDFIICCGYKGYVI
CCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCHH
KEYFANYFLHMSDVTFDMKNNEMKVHCERAEPWTVTLIDTGDASMTGGRLARVADHIKDE
HHHHHHHEEEECCEEEEECCCEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHCCCC
EAFCFTYGDGVSDVDITKTIEFHKSHGKKATLTATFPPGRFGALDITAGKVNSFKEKPRG
CEEEEEECCCCCCCEEHEEEEEHHCCCCEEEEEEECCCCCCCEEEEECCCCHHHHCCCCC
DGALINGGFFVLSPEVLKLIDGDSCTWEQEPLKTLAQENELMSYEHQGFWQPMDTLRDKV
CCEEEECCEEEECHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
HLEELWQAGNAPWKIWE
HHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]