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The map label for this gene is purR [H]

Identifier: 119944130

GI number: 119944130

Start: 446599

End: 447603

Strand: Direct

Name: purR [H]

Synonym: Ping_0345

Alternate gene names: 119944130

Gene position: 446599-447603 (Clockwise)

Preceding gene: 119944129

Following gene: 119944138

Centisome position: 9.79

GC content: 41.69

Gene sequence:

>1005_bases
ATGGCAACCATAAAAGATGTAGCAAAACATGCGGGCGTGTCTACTTCAACGGTCAGCCATGTTTTAAATCAAACACGTTA
TGTCAGCGAAGATATCACGGCTCGCGTAAAAAAGGCGGTCGATGAGCTGCAATATGCGCCGTCGGCCTTAGCGAGAAGCC
TTAAGCTTAAATATACACATACCTTTGGTATGTTAGTGACAACCTCAGCGAACCCCTTTTTTGCGGAAGTGGTAAAGGGG
GTTGAGCGCCGCTGTTATGAGCAAGGTTATAATTTACTGCTGTGTAATACGGAAGGCGATTTAGAGCGCTTACGTTTTAA
TATCGATATGCTGCTCCAAAAAAGGGTGGATGGACTATTACTGATGAGTGATGAAATCGTCAGTCAAAACTTAGATATTT
TTGCCCGCCATAAAGCCGTGCCGACTGTTGTTATGGATTGTGGTAAAACGCATTTTCCGGCAGATAAAATCCAAGATAAT
TCCTATCTCGGTGGTTACTTAGCAACCCAGTATCTGATAAAAAAAGGTCATAGAAAAATTGGCTGTATTACTGGACCTAT
CAATAAACCCATGGCTGAAAAACGTTGGGCGGGTTATCAAAAAGCGATGCAAGATGCCGGGCTTATTATCAATCCTGATT
GGCTGGTGGATGGCAATTTTGAATGTGAAGGGGGTCAACAAGCCTTAGATAAAATGTTGAGTAGGGGCACTTTACCCAGC
GCACTTTTTGTTTGCAACGATATGATGGCATTGGGTGTTATTAACAGCGCCAGCAAACATGGCATCTCGATTCCCGATGA
TTTATCTATTATTGGCTATGATGATATAAAAATAGCCCGATTTTTCACTCCCTCATTGACCACTATCCATCAACCTAAAT
TTAATTTAGGGCGAAAAGCCTTTGATACCCTGTTAGATAAGATTCAAAGCAAAAGAGAAAATGATTTAGAGATAAAGCTG
GAACCGACATTAATTGAGCGGGACAGTGTAAAGCAGATAGGTTAA

Upstream 100 bases:

>100_bases
CGGTAAAGGGGCACAAACATCGATTCCAGATCGCCAGAGTGTAGAGAAATTTTTACAGCAGATGGACAATAAATGATTAT
TTAAGGATTTGAACAAGCAA

Downstream 100 bases:

>100_bases
ATTCAGCTGTCAGCTGTCAGCTGTCAGCCGATAGCCGTCAGCCAACAGCCGTCAGTTTCTATTTCAGCTTGCTTATATCG
CGTATTGCGCCTTTATCGGC

Product: transcriptional regulator for D-ribose, periplasmic binding protein, LacI family protein

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MATIKDVAKHAGVSTSTVSHVLNQTRYVSEDITARVKKAVDELQYAPSALARSLKLKYTHTFGMLVTTSANPFFAEVVKG
VERRCYEQGYNLLLCNTEGDLERLRFNIDMLLQKRVDGLLLMSDEIVSQNLDIFARHKAVPTVVMDCGKTHFPADKIQDN
SYLGGYLATQYLIKKGHRKIGCITGPINKPMAEKRWAGYQKAMQDAGLIINPDWLVDGNFECEGGQQALDKMLSRGTLPS
ALFVCNDMMALGVINSASKHGISIPDDLSIIGYDDIKIARFFTPSLTTIHQPKFNLGRKAFDTLLDKIQSKRENDLEIKL
EPTLIERDSVKQIG

Sequences:

>Translated_334_residues
MATIKDVAKHAGVSTSTVSHVLNQTRYVSEDITARVKKAVDELQYAPSALARSLKLKYTHTFGMLVTTSANPFFAEVVKG
VERRCYEQGYNLLLCNTEGDLERLRFNIDMLLQKRVDGLLLMSDEIVSQNLDIFARHKAVPTVVMDCGKTHFPADKIQDN
SYLGGYLATQYLIKKGHRKIGCITGPINKPMAEKRWAGYQKAMQDAGLIINPDWLVDGNFECEGGQQALDKMLSRGTLPS
ALFVCNDMMALGVINSASKHGISIPDDLSIIGYDDIKIARFFTPSLTTIHQPKFNLGRKAFDTLLDKIQSKRENDLEIKL
EPTLIERDSVKQIG
>Mature_333_residues
ATIKDVAKHAGVSTSTVSHVLNQTRYVSEDITARVKKAVDELQYAPSALARSLKLKYTHTFGMLVTTSANPFFAEVVKGV
ERRCYEQGYNLLLCNTEGDLERLRFNIDMLLQKRVDGLLLMSDEIVSQNLDIFARHKAVPTVVMDCGKTHFPADKIQDNS
YLGGYLATQYLIKKGHRKIGCITGPINKPMAEKRWAGYQKAMQDAGLIINPDWLVDGNFECEGGQQALDKMLSRGTLPSA
LFVCNDMMALGVINSASKHGISIPDDLSIIGYDDIKIARFFTPSLTTIHQPKFNLGRKAFDTLLDKIQSKRENDLEIKLE
PTLIERDSVKQIG

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=54.1033434650456, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=47.5903614457831, Blast_Score=331, Evalue=4e-92,
Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=32.5301204819277, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=33.1325301204819, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788474, Length=338, Percent_Identity=31.9526627218935, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1789068, Length=294, Percent_Identity=31.9727891156463, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=29.2834890965732, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786540, Length=310, Percent_Identity=30, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=26.5822784810127, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787906, Length=332, Percent_Identity=25.3012048192771, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789456, Length=327, Percent_Identity=26.605504587156, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI48994940, Length=309, Percent_Identity=24.5954692556634, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786268, Length=321, Percent_Identity=22.7414330218069, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790689, Length=315, Percent_Identity=22.5396825396825, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790192, Length=270, Percent_Identity=25.1851851851852, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37257; Mature: 37126

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKHAGVSTSTVSHVLNQTRYVSEDITARVKKAVDELQYAPSALARSLKLKYTH
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEEEEE
TFGMLVTTSANPFFAEVVKGVERRCYEQGYNLLLCNTEGDLERLRFNIDMLLQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEE
LMSDEIVSQNLDIFARHKAVPTVVMDCGKTHFPADKIQDNSYLGGYLATQYLIKKGHRKI
EECHHHHHCCCCCEEHHHCCCHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCEE
GCITGPINKPMAEKRWAGYQKAMQDAGLIINPDWLVDGNFECEGGQQALDKMLSRGTLPS
EEEECCCCCCHHHHHHHHHHHHHHCCCCEECCCEEECCCEECCCHHHHHHHHHHCCCCCH
ALFVCNDMMALGVINSASKHGISIPDDLSIIGYDDIKIARFFTPSLTTIHQPKFNLGRKA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCEEECCCHHCCHHH
FDTLLDKIQSKRENDLEIKLEPTLIERDSVKQIG
HHHHHHHHHHCCCCCCEEEECCCEECCCCHHCCC
>Mature Secondary Structure 
ATIKDVAKHAGVSTSTVSHVLNQTRYVSEDITARVKKAVDELQYAPSALARSLKLKYTH
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEEEEE
TFGMLVTTSANPFFAEVVKGVERRCYEQGYNLLLCNTEGDLERLRFNIDMLLQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEE
LMSDEIVSQNLDIFARHKAVPTVVMDCGKTHFPADKIQDNSYLGGYLATQYLIKKGHRKI
EECHHHHHCCCCCEEHHHCCCHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCEE
GCITGPINKPMAEKRWAGYQKAMQDAGLIINPDWLVDGNFECEGGQQALDKMLSRGTLPS
EEEECCCCCCHHHHHHHHHHHHHHCCCCEECCCEEECCCEECCCHHHHHHHHHHCCCCCH
ALFVCNDMMALGVINSASKHGISIPDDLSIIGYDDIKIARFFTPSLTTIHQPKFNLGRKA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCEEECCCHHCCHHH
FDTLLDKIQSKRENDLEIKLEPTLIERDSVKQIG
HHHHHHHHHHCCCCCCEEEECCCEECCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]