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The map label for this gene is pilT [H]

Identifier: 119899757

GI number: 119899757

Start: 3804339

End: 3805379

Strand: Direct

Name: pilT [H]

Synonym: azo3468

Alternate gene names: 119899757

Gene position: 3804339-3805379 (Clockwise)

Preceding gene: 119899752

Following gene: 119899758

Centisome position: 86.94

GC content: 63.78

Gene sequence:

>1041_bases
ATGGACATCACCGAACTGCTCGCCTTCGCGGTCAAGAACAAGGCTTCCGACCTGCATCTCTCCGCCGGGCTGCCGCCCAT
GATCCGGGTGCACGGCGACGTGCGCCGCATCAATCTGCCGCCCATGGAGCACAAGGACGTGCACGCCATGGTGTACGACA
TCATGAACGACGGCCAGCGCAAGCAGTTCGAGGAAAGCTGGGAGTGCGACTTCTCGTTCGCGGTGCCCAATCTGGCCCGC
TTCCGGGTCAACGCGTTCAACCAGAACCGTGGCGCCGGCGCCGTGTTCCGGACCATTCCGTCCAAGGTGCTGACGCTCGA
AGAACTGAACTGCCCGAAGATCTTCAAGGACATCGCGAACCAGCCGCGCGGCATCGTGCTGGTCACCGGGCCGACGGGCT
CGGGCAAATCAACCACGCTCGCGGCGATGGTGGACTACATCAACGAGAACGAATACGGCCACATCCTCACGGTCGAGGAC
CCGATCGAATTCGTCCATGAATCCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCAGTCGTTCAACAA
CGCGCTGCGCGCTTCCCTGCGCGAAGATCCGGACGTGATCCTGGTCGGCGAAATGCGCGACCTCGAAACCATCCGTCTCG
CGCTCACCGCGGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCGGCCGCGGAAAAGGACATGGTGCGCTCGATGCTTTCCGAGTCGCTGCGCGCGGTCATCTCGCAAAC
CCTGCTGAAGACCAAGGACGGGAGCGGTCGCGTGGCCGCCCACGAGATCATGATCGGCTCGCCCGCCATCCGTAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGAATCTGGGCATGCAGACGCTGGACCAGTGC
CTCGCCGACCTCGTCCGCCGCAACGTCGTCTCCGCGGCAGAAGCGCGCGTGCGTGCGCAGAACAAGGACAACTTCGCCTG
A

Upstream 100 bases:

>100_bases
CATTGCGCCGAAAGTATAGCACCGGCCCCCTGCACGCCCGCCCCGGCGCGCCCTGCTCCCACCGCCTCAGCCCTCCCCGC
TGCCTTCACGATCGAACTCC

Downstream 100 bases:

>100_bases
GCGCGGTCCGGCATCTGCCGCCCGACCCGCACCCCTAAAGATCAACCGCCCGCCGCAAGGCAGCCCGCCCCCGCCAGGAG
TCCGCCATGGAACGCGACCA

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKDVHAMVYDIMNDGQRKQFEESWECDFSFAVPNLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEELNCPKIFKDIANQPRGIVLVTGPTGSGKSTTLAAMVDYINENEYGHILTVED
PIEFVHESKRCLINQREVHRDTQSFNNALRASLREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGSGRVAAHEIMIGSPAIRNLIRENKIAQMYSAIQTGQNLGMQTLDQC
LADLVRRNVVSAAEARVRAQNKDNFA

Sequences:

>Translated_346_residues
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKDVHAMVYDIMNDGQRKQFEESWECDFSFAVPNLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEELNCPKIFKDIANQPRGIVLVTGPTGSGKSTTLAAMVDYINENEYGHILTVED
PIEFVHESKRCLINQREVHRDTQSFNNALRASLREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGSGRVAAHEIMIGSPAIRNLIRENKIAQMYSAIQTGQNLGMQTLDQC
LADLVRRNVVSAAEARVRAQNKDNFA
>Mature_346_residues
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKDVHAMVYDIMNDGQRKQFEESWECDFSFAVPNLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEELNCPKIFKDIANQPRGIVLVTGPTGSGKSTTLAAMVDYINENEYGHILTVED
PIEFVHESKRCLINQREVHRDTQSFNNALRASLREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGSGRVAAHEIMIGSPAIRNLIRENKIAQMYSAIQTGQNLGMQTLDQC
LADLVRRNVVSAAEARVRAQNKDNFA

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=319, Percent_Identity=48.9028213166144, Blast_Score=307, Evalue=8e-85,
Organism=Escherichia coli, GI1789723, Length=350, Percent_Identity=32.2857142857143, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786296, Length=369, Percent_Identity=31.7073170731707, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38474; Mature: 38474

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKDVHAMVYDIMNDGQR
CCHHHHHHHHHHCCCCCEEECCCCCCEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCH
KQFEESWECDFSFAVPNLARFRVNAFNQNRGAGAVFRTIPSKVLTLEELNCPKIFKDIAN
HHHHHHCCCCEEECCCCHHHHEEECCCCCCCCCHHHHHHHHHHEEHHHCCCHHHHHHHCC
QPRGIVLVTGPTGSGKSTTLAAMVDYINENEYGHILTVEDPIEFVHESKRCLINQREVHR
CCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
DTQSFNNALRASLREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
HHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHEEEEECCCCCEEEEEEECCHHHHHHHHH
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGSGRVAAHEIMIGSPAIRNLIRENKIA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHH
QMYSAIQTGQNLGMQTLDQCLADLVRRNVVSAAEARVRAQNKDNFA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKDVHAMVYDIMNDGQR
CCHHHHHHHHHHCCCCCEEECCCCCCEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCH
KQFEESWECDFSFAVPNLARFRVNAFNQNRGAGAVFRTIPSKVLTLEELNCPKIFKDIAN
HHHHHHCCCCEEECCCCHHHHEEECCCCCCCCCHHHHHHHHHHEEHHHCCCHHHHHHHCC
QPRGIVLVTGPTGSGKSTTLAAMVDYINENEYGHILTVEDPIEFVHESKRCLINQREVHR
CCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
DTQSFNNALRASLREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
HHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHEEEEECCCCCEEEEEEECCHHHHHHHHH
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGSGRVAAHEIMIGSPAIRNLIRENKIA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHH
QMYSAIQTGQNLGMQTLDQCLADLVRRNVVSAAEARVRAQNKDNFA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]