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The map label for this gene is pilU2 [H]

Identifier: 119899758

GI number: 119899758

Start: 3805466

End: 3806599

Strand: Direct

Name: pilU2 [H]

Synonym: azo3469

Alternate gene names: 119899758

Gene position: 3805466-3806599 (Clockwise)

Preceding gene: 119899757

Following gene: 119899759

Centisome position: 86.96

GC content: 62.87

Gene sequence:

>1134_bases
ATGGAACGCGACCAGGCCCTGAAATTCATGCACGACCTGCTGCGGCTGATGCTGCAGAAGAACGGTTCCGACCTCTTCAT
CAATCCCGGCTTTCCGCCGGCGATCAAGGTCGACGGACGCATCGTGCCGCAGTCCAACCAGGCGCTGGCGCCGCAGCACA
CCGCGGAACTTGCGCGCGCGATCATGAACGACCGCCAGTCGGCCGAATTCGAAGCCACCAAGGAGTGCAACTTCGCGATC
TCGCCCGCGGGCATCGGCCGCTTCCGGGCCAATGCCTACATCCAGCAAGGCCGCGTGGCGCTGGTGTTGCGCACGATCGC
GCAGAAGATCCCGACCATCGACGAACTCGGCCTGCCGCCGGTATTGCGCGACATCGGCATGACCAAGCGCGGCCTGGTGA
TCTTCGTCGGCGGCACCGGCACCGGCAAGACCACCTCGCTGGCGGCGATGGTCGACTTCCGCAATGAGAACAGCTACGGC
CACATCATCACGATCGAAGATCCGATCGAGTACGTGCACCCGCACAAGAACTGCATCGTGTCGCAACGCGAAGTCGGCAT
CGACACCGACAACTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAAATCCGTG
ACCGCGAAACCATGGATTACGCGATCGCCTTCGCCGAAACCGGCCACCTCTGCCTTGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGATCGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCCAGCAACTGCTGATGGATTTGTCGCTGAACCT
GCGGGCGATGGTTTCGCAGCGCCTGCTGCCGCTCAAGGGGCGCAAGGGCCGCGTGCCCGCGGTCGAGGTGCTGCTCAACT
CCCCGCTGGTGTCGGACCTGATCTTCAAGGGCGAGGTGCCAGGCATCAAGGAGATCATGAAGCGCTCCCGCGAACTGGGC
ATGCAGACCTTCGACCAGGCCTTGTTCGACCTCTACGAAGCCGAGCTGATCAGCTACGAAGACGCGTTGCGCAATGCCGA
TTCGGTGAATGACCTGCGGCTGCAGATCAAGCTCAACAGCAAGCTGGGCGACAAGGACCTCGTCTCGGGTATCCAGAACC
TCGACATCGTCTGA

Upstream 100 bases:

>100_bases
ACAACTTCGCCTGAGCGCGGTCCGGCATCTGCCGCCCGACCCGCACCCCTAAAGATCAACCGCCCGCCGCAAGGCAGCCC
GCCCCCGCCAGGAGTCCGCC

Downstream 100 bases:

>100_bases
ATTCCGAGCGCCAGCGCAGCCCCGCGACAGCGGCACCTCAGGTGCCGCTTTTTTTTGGTCCGCGCTTTCGCCGGGGCGTT
GGCGGGACCGACGCAGCGGC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MERDQALKFMHDLLRLMLQKNGSDLFINPGFPPAIKVDGRIVPQSNQALAPQHTAELARAIMNDRQSAEFEATKECNFAI
SPAGIGRFRANAYIQQGRVALVLRTIAQKIPTIDELGLPPVLRDIGMTKRGLVIFVGGTGTGKTTSLAAMVDFRNENSYG
HIITIEDPIEYVHPHKNCIVSQREVGIDTDNWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRQQLLMDLSLNLRAMVSQRLLPLKGRKGRVPAVEVLLNSPLVSDLIFKGEVPGIKEIMKRSRELG
MQTFDQALFDLYEAELISYEDALRNADSVNDLRLQIKLNSKLGDKDLVSGIQNLDIV

Sequences:

>Translated_377_residues
MERDQALKFMHDLLRLMLQKNGSDLFINPGFPPAIKVDGRIVPQSNQALAPQHTAELARAIMNDRQSAEFEATKECNFAI
SPAGIGRFRANAYIQQGRVALVLRTIAQKIPTIDELGLPPVLRDIGMTKRGLVIFVGGTGTGKTTSLAAMVDFRNENSYG
HIITIEDPIEYVHPHKNCIVSQREVGIDTDNWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRQQLLMDLSLNLRAMVSQRLLPLKGRKGRVPAVEVLLNSPLVSDLIFKGEVPGIKEIMKRSRELG
MQTFDQALFDLYEAELISYEDALRNADSVNDLRLQIKLNSKLGDKDLVSGIQNLDIV
>Mature_377_residues
MERDQALKFMHDLLRLMLQKNGSDLFINPGFPPAIKVDGRIVPQSNQALAPQHTAELARAIMNDRQSAEFEATKECNFAI
SPAGIGRFRANAYIQQGRVALVLRTIAQKIPTIDELGLPPVLRDIGMTKRGLVIFVGGTGTGKTTSLAAMVDFRNENSYG
HIITIEDPIEYVHPHKNCIVSQREVGIDTDNWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRQQLLMDLSLNLRAMVSQRLLPLKGRKGRVPAVEVLLNSPLVSDLIFKGEVPGIKEIMKRSRELG
MQTFDQALFDLYEAELISYEDALRNADSVNDLRLQIKLNSKLGDKDLVSGIQNLDIV

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=321, Percent_Identity=36.4485981308411, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786296, Length=376, Percent_Identity=27.3936170212766, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789723, Length=147, Percent_Identity=37.4149659863946, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42066; Mature: 42066

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERDQALKFMHDLLRLMLQKNGSDLFINPGFPPAIKVDGRIVPQSNQALAPQHTAELARA
CCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEECCEEECCCCCCCCCHHHHHHHHH
IMNDRQSAEFEATKECNFAISPAGIGRFRANAYIQQGRVALVLRTIAQKIPTIDELGLPP
HHCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCH
VLRDIGMTKRGLVIFVGGTGTGKTTSLAAMVDFRNENSYGHIITIEDPIEYVHPHKNCIV
HHHHCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCEEEEEECCCHHHHCCCCHHEE
SQREVGIDTDNWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
CHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHEEEEECCCCEEEEEEECCCH
NQAIDRIINFFPEDRRQQLLMDLSLNLRAMVSQRLLPLKGRKGRVPAVEVLLNSPLVSDL
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHH
IFKGEVPGIKEIMKRSRELGMQTFDQALFDLYEAELISYEDALRNADSVNDLRLQIKLNS
HHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
KLGDKDLVSGIQNLDIV
CCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MERDQALKFMHDLLRLMLQKNGSDLFINPGFPPAIKVDGRIVPQSNQALAPQHTAELARA
CCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEECCEEECCCCCCCCCHHHHHHHHH
IMNDRQSAEFEATKECNFAISPAGIGRFRANAYIQQGRVALVLRTIAQKIPTIDELGLPP
HHCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCH
VLRDIGMTKRGLVIFVGGTGTGKTTSLAAMVDFRNENSYGHIITIEDPIEYVHPHKNCIV
HHHHCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCEEEEEECCCHHHHCCCCHHEE
SQREVGIDTDNWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
CHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHEEEEECCCCEEEEEEECCCH
NQAIDRIINFFPEDRRQQLLMDLSLNLRAMVSQRLLPLKGRKGRVPAVEVLLNSPLVSDL
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHH
IFKGEVPGIKEIMKRSRELGMQTFDQALFDLYEAELISYEDALRNADSVNDLRLQIKLNS
HHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
KLGDKDLVSGIQNLDIV
CCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]