Click here to switch to the map view.

The map label for this gene is pyrX [H]

Identifier: 119899752

GI number: 119899752

Start: 3800410

End: 3801693

Strand: Direct

Name: pyrX [H]

Synonym: azo3463

Alternate gene names: 119899752

Gene position: 3800410-3801693 (Clockwise)

Preceding gene: 119899751

Following gene: 119899757

Centisome position: 86.85

GC content: 69.7

Gene sequence:

>1284_bases
ATGAAGATTCAGATCACCAACGGCCGGCTGATCGACCCGGCCAACAACATCGACGCGCAGCAGGATGTGTACATCGTCGG
CGGGCGCGTGGCCGGCCTCGGCCGTGCTCCCGAGGGCTTCCAGGCCGACCGCGTCATCGACGCCAGCGGCCTGGTGGTTG
CCCCCGGCCTGATCGACCTCGCCGCGCGCCTGCGCGAGCCGGGCTTCGAATACCGCGCCACGCTGGAATCCGAGATGGAC
GCGGCGATGGCGGGCGGCGTCACCAGCCTCGCCATTCCGCCCGACACCGACCCCGCGCTGGACGAGCCCGGCCTGGTCGA
AATGCTGTGCTACCGCGCCAAGAAACTGAACCGCGCCCATGTGTACCCGGTCGGCGCGCTCACCATCGGGCTGAAGGGCG
AACGCCTGTCGGAAATGGCGGAACTGGTCGAGGCCGGCTGCGTCGCCTTCAGCCAGGCTAACGTGCCCATCGTGGACAAT
GCGGTGCTGATGCGGGCGATGCAGTATGCCGCCACCTTCGGGTTTCGCGTCTGGCTGCAGCCGATCGCGCCCTTCCTCGC
GCGTGGCGGCTACGCCCACGACGGCGAATTCGCCAGCCGCATCGGCCTGCCGGGCATCCCGGTCGCCGCGGAGACGGTGG
CGCTCTACACCTACCTGCAGCTGGCGGGCATGACGGGTGCGCGCCTGCACATCACCCGCCTGTCGAGCGCGGCCGGCCTC
GCGCTGATCGAACAGGCCCGCGCCGACGGCATGGACGTGACCTGCGACGTGTCGATCAACCACCTGCATCTGTGCGACAC
CGACATCGGCTACTTCAACGCCAACTGTCATCTGGTCCCGCCGCTGCGCAGCCAGCGCGATCGCGAGGCGCTGTGCCGCG
GCCTGGCGGACGGCCGCATCAACGCGCTGTGTTCGGACCACACGCCGGTGGACGACGACGGCAAGCTGACGCCGTTCAGC
GAATCCGAGGCCGGCGCCACCGGGCTGGAACTGCTACTGCCGCTGACGCTGAAATGGGCGCAGGAGGCGGGGGTGCCGCT
GCCGCTGGCGCTGGGCCGCATCACGACCGATGCGGCGCGCATCGTCGGCATCACCAAGGCCGGACATCTGGGCGTGGGCG
CGCGCGCCGACATCTGCGTGTTCGATCCCGCCGCCTACGTGAAGATCAGCCGCGAAGGCCTGCGCAGCCAGGGCAAGAAC
ACGCCCTTCCTCGGGCTCGAACTCCCCGGGCAGGTGCGCTACACGCTGGTCGAAGGCCAGGTGATGTTCGCGCGCGCCGA
CTGA

Upstream 100 bases:

>100_bases
GCGGTGATGAGCATGCTCGGCAGCCACTGATCGCGCCTCGCGGGCACAGGCCGGCCGCCTGCGCAGGGCGGCGGGCACGC
GCACTCACAAGGAATCACGG

Downstream 100 bases:

>100_bases
GCCGCCTCAGCCCAGCGCACGCAGACGCCCGGCCAGTCCGGGCGTTTTCGTTTCCACCCTGACCCGCTTCGCGCGCGAGG
TCTCGCCACTGACGAGGGTC

Product: dihydroorotase

Products: N-carbamoyl-L-aspartate

Alternate protein names: Aspartate carbamoyltransferase 44 kDa Non-catalytic chain [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKIQITNGRLIDPANNIDAQQDVYIVGGRVAGLGRAPEGFQADRVIDASGLVVAPGLIDLAARLREPGFEYRATLESEMD
AAMAGGVTSLAIPPDTDPALDEPGLVEMLCYRAKKLNRAHVYPVGALTIGLKGERLSEMAELVEAGCVAFSQANVPIVDN
AVLMRAMQYAATFGFRVWLQPIAPFLARGGYAHDGEFASRIGLPGIPVAAETVALYTYLQLAGMTGARLHITRLSSAAGL
ALIEQARADGMDVTCDVSINHLHLCDTDIGYFNANCHLVPPLRSQRDREALCRGLADGRINALCSDHTPVDDDGKLTPFS
ESEAGATGLELLLPLTLKWAQEAGVPLPLALGRITTDAARIVGITKAGHLGVGARADICVFDPAAYVKISREGLRSQGKN
TPFLGLELPGQVRYTLVEGQVMFARAD

Sequences:

>Translated_427_residues
MKIQITNGRLIDPANNIDAQQDVYIVGGRVAGLGRAPEGFQADRVIDASGLVVAPGLIDLAARLREPGFEYRATLESEMD
AAMAGGVTSLAIPPDTDPALDEPGLVEMLCYRAKKLNRAHVYPVGALTIGLKGERLSEMAELVEAGCVAFSQANVPIVDN
AVLMRAMQYAATFGFRVWLQPIAPFLARGGYAHDGEFASRIGLPGIPVAAETVALYTYLQLAGMTGARLHITRLSSAAGL
ALIEQARADGMDVTCDVSINHLHLCDTDIGYFNANCHLVPPLRSQRDREALCRGLADGRINALCSDHTPVDDDGKLTPFS
ESEAGATGLELLLPLTLKWAQEAGVPLPLALGRITTDAARIVGITKAGHLGVGARADICVFDPAAYVKISREGLRSQGKN
TPFLGLELPGQVRYTLVEGQVMFARAD
>Mature_427_residues
MKIQITNGRLIDPANNIDAQQDVYIVGGRVAGLGRAPEGFQADRVIDASGLVVAPGLIDLAARLREPGFEYRATLESEMD
AAMAGGVTSLAIPPDTDPALDEPGLVEMLCYRAKKLNRAHVYPVGALTIGLKGERLSEMAELVEAGCVAFSQANVPIVDN
AVLMRAMQYAATFGFRVWLQPIAPFLARGGYAHDGEFASRIGLPGIPVAAETVALYTYLQLAGMTGARLHITRLSSAAGL
ALIEQARADGMDVTCDVSINHLHLCDTDIGYFNANCHLVPPLRSQRDREALCRGLADGRINALCSDHTPVDDDGKLTPFS
ESEAGATGLELLLPLTLKWAQEAGVPLPLALGRITTDAARIVGITKAGHLGVGARADICVFDPAAYVKISREGLRSQGKN
TPFLGLELPGQVRYTLVEGQVMFARAD

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. PyrC' subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=380, Percent_Identity=28.6842105263158, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1786722, Length=433, Percent_Identity=27.9445727482679, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87082175, Length=449, Percent_Identity=25.1670378619154, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=399, Percent_Identity=23.5588972431078, Blast_Score=88, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6322218, Length=421, Percent_Identity=26.3657957244656, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI18859883, Length=408, Percent_Identity=25.9803921568627, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.3

Molecular weight: Translated: 45437; Mature: 45437

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQITNGRLIDPANNIDAQQDVYIVGGRVAGLGRAPEGFQADRVIDASGLVVAPGLIDL
CEEEEECCEEECCCCCCCCCCCEEEECCEEECCCCCCCCCCCCEEECCCCCEECCCHHHH
AARLREPGFEYRATLESEMDAAMAGGVTSLAIPPDTDPALDEPGLVEMLCYRAKKLNRAH
HHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEE
VYPVGALTIGLKGERLSEMAELVEAGCVAFSQANVPIVDNAVLMRAMQYAATFGFRVWLQ
EEEEEEEEEECCCHHHHHHHHHHHHHHHEEECCCCCEECHHHHHHHHHHHHHHHHHHHHH
PIAPFLARGGYAHDGEFASRIGLPGIPVAAETVALYTYLQLAGMTGARLHITRLSSAAGL
HHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
ALIEQARADGMDVTCDVSINHLHLCDTDIGYFNANCHLVPPLRSQRDREALCRGLADGRI
HHHHHHHCCCCCEEEEEEECEEEEECCCCCEECCCCEEECCHHCCCCHHHHHHHCCCCCE
NALCSDHTPVDDDGKLTPFSESEAGATGLELLLPLTLKWAQEAGVPLPLALGRITTDAAR
EEEECCCCCCCCCCCCCCCCCCCCCCCCHHEEHHHHHHHHHHCCCCCEEECCCCCCCCCC
IVGITKAGHLGVGARADICVFDPAAYVKISREGLRSQGKNTPFLGLELPGQVRYTLVEGQ
EEEEECCCCCCCCCCCCEEEECCHHEEEEEHHHHHHCCCCCCEEEEECCCCEEEEEEECE
VMFARAD
EEEEECC
>Mature Secondary Structure
MKIQITNGRLIDPANNIDAQQDVYIVGGRVAGLGRAPEGFQADRVIDASGLVVAPGLIDL
CEEEEECCEEECCCCCCCCCCCEEEECCEEECCCCCCCCCCCCEEECCCCCEECCCHHHH
AARLREPGFEYRATLESEMDAAMAGGVTSLAIPPDTDPALDEPGLVEMLCYRAKKLNRAH
HHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEE
VYPVGALTIGLKGERLSEMAELVEAGCVAFSQANVPIVDNAVLMRAMQYAATFGFRVWLQ
EEEEEEEEEECCCHHHHHHHHHHHHHHHEEECCCCCEECHHHHHHHHHHHHHHHHHHHHH
PIAPFLARGGYAHDGEFASRIGLPGIPVAAETVALYTYLQLAGMTGARLHITRLSSAAGL
HHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
ALIEQARADGMDVTCDVSINHLHLCDTDIGYFNANCHLVPPLRSQRDREALCRGLADGRI
HHHHHHHCCCCCEEEEEEECEEEEECCCCCEECCCCEEECCHHCCCCHHHHHHHCCCCCE
NALCSDHTPVDDDGKLTPFSESEAGATGLELLLPLTLKWAQEAGVPLPLALGRITTDAAR
EEEECCCCCCCCCCCCCCCCCCCCCCCCHHEEHHHHHHHHHHCCCCCEEECCCCCCCCCC
IVGITKAGHLGVGARADICVFDPAAYVKISREGLRSQGKNTPFLGLELPGQVRYTLVEGQ
EEEEECCCCCCCCCCCCEEEECCHHEEEEEHHHHHHCCCCCCEEEEECCCCEEEEEEECE
VMFARAD
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-dihydroorotate; H2O

Specific reaction: (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]