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The map label for this gene is phoQ [H]

Identifier: 119776436

GI number: 119776436

Start: 3929960

End: 3931360

Strand: Direct

Name: phoQ [H]

Synonym: Sama_3304

Alternate gene names: 119776436

Gene position: 3929960-3931360 (Clockwise)

Preceding gene: 119776435

Following gene: 119776440

Centisome position: 91.26

GC content: 60.31

Gene sequence:

>1401_bases
ATGTCACACTCAGCCTCTACGCCCAAGCCAGTGCCTGCAGCCGCACCGGCCCACAAGCAGGCGGCGGCCTTTATCGATCG
CCTATCCCTGAAAACACGCCTCTTCGTCAGTGCCGCCATACTGGTGGCATTGCTGCTGCCCGCCCTTGGCATTGCCCTGT
TTCAGGCGTTTGAACGTCAGGCACTGGCAGGCAGCCGTGACGAGCTCGGCGCCCTGATTTACTCGGTGCTGGCACAAACC
GACGTGATAGACGGCAAGCTGGAAATGCCGCCCTTCCTCAATGAACCCCAGTTCAATGTCGATGGCTCCGGCCTCTATGC
CGAGGTGCGCTCAGGCGCAGGCGAAGTGCTGTGGCGCTCAGGCTCCCTCATTGGCCATGGCGCCATCCCGGCACTGCCAA
GCCCCGCCCCCGGCGCCGATGCCAAGGTGTTTGGCGAAACCCTGCTTAACGACGAGCCGCTGTTTGTGATGAGCTTTACC
GCCGTCTACGAGAGCAAGGGCGTGGACGTGCCCCTGAGTATCCATATTCTTAAATCCCAGCAGCGTTACCGCGAAGCCCT
CACCGAATTTGAGTCCAGCCTGTGGCGTTATCTGCTGCTGGCACTGGCGCTGCTCGCCGTGGTGCTGGGCCTGTGGCTGC
GCTGGACTCTAAAGCCGCTGTCACGCTTCGAGGCGGAACTCAAAGCGGTGGAGCACGGCGACAAAGAACGCATAGACACC
GATTATCCCACCGAGCTTGGGCCGCTGGTGCATCAGCTCAACTCCCTGTTAACCACAGAGCAAAACCAGCGTAAACGCTA
CCGCAATGCCCTGTCGGATCTGGCCCACAGCTTAAAAACCCCGCTGGCGGTGCTGCAAAGCCACCCGGGGCTGGACGATG
CCGTGATGGAGCAGGTGGACAACATCAGCCACAGCATCAGCCATCAGCTTAAACGGGCCCAGAGCGCCGGCGCCGCCTCC
TGGCACCAGGGGGTGGAAATCCTGCCCCAGGCAGAGCGGTTAACGCGCACCTTAAGCAAAATCCATGGCGATAAGGGCAT
CGACTTCGAGCTTAAACTCGCGGCCGAACTGGTGTTTAAGGGCGATAAAGGGGATTTGACTGAGCTTCTGGGCAATCTTT
TGGATAACGCCTGCAAGGCCGCCCGCTCTCGGGTGTGCTTAAGCGCCGCCAAGCAGGATGGCAGGCTGCGGCTTATCGTC
GAGGATGATGGCCCCGGGGTGGATGAGTCGATGCGGGATAAGATTTTTGAGCGCGGCATTCGTGCCGATACCTACGACAA
AGGCCATGGGATAGGCCTTGCGATAGTGCAGGATCTTACCGATGCCTATCAGGGACGCTTGCTGCTTGGCCGCAGTGATT
TGCTCGGCGGCGCGCGGTTTGAGGTGTCTTTCCCCCAGTAA

Upstream 100 bases:

>100_bases
TCTTTATCCGCCGGTTGCGTAAAAAGCTCGACCCGGACAACGAGCTCGGGCTGATTGAAACCCTGCGCGGCCAGGGCTAC
CGGCTGAACCTGCTGACCTG

Downstream 100 bases:

>100_bases
CTTGTTTTCGCCTGACTTGGCGATGGGAAGTTTTTTGAAGTCGCTTTGCAAGCGACGCAAATCGAAGCCTTCCAGGCTTC
ACCGGGGCAAAGGGCTTTGC

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSHSASTPKPVPAAAPAHKQAAAFIDRLSLKTRLFVSAAILVALLLPALGIALFQAFERQALAGSRDELGALIYSVLAQT
DVIDGKLEMPPFLNEPQFNVDGSGLYAEVRSGAGEVLWRSGSLIGHGAIPALPSPAPGADAKVFGETLLNDEPLFVMSFT
AVYESKGVDVPLSIHILKSQQRYREALTEFESSLWRYLLLALALLAVVLGLWLRWTLKPLSRFEAELKAVEHGDKERIDT
DYPTELGPLVHQLNSLLTTEQNQRKRYRNALSDLAHSLKTPLAVLQSHPGLDDAVMEQVDNISHSISHQLKRAQSAGAAS
WHQGVEILPQAERLTRTLSKIHGDKGIDFELKLAAELVFKGDKGDLTELLGNLLDNACKAARSRVCLSAAKQDGRLRLIV
EDDGPGVDESMRDKIFERGIRADTYDKGHGIGLAIVQDLTDAYQGRLLLGRSDLLGGARFEVSFPQ

Sequences:

>Translated_466_residues
MSHSASTPKPVPAAAPAHKQAAAFIDRLSLKTRLFVSAAILVALLLPALGIALFQAFERQALAGSRDELGALIYSVLAQT
DVIDGKLEMPPFLNEPQFNVDGSGLYAEVRSGAGEVLWRSGSLIGHGAIPALPSPAPGADAKVFGETLLNDEPLFVMSFT
AVYESKGVDVPLSIHILKSQQRYREALTEFESSLWRYLLLALALLAVVLGLWLRWTLKPLSRFEAELKAVEHGDKERIDT
DYPTELGPLVHQLNSLLTTEQNQRKRYRNALSDLAHSLKTPLAVLQSHPGLDDAVMEQVDNISHSISHQLKRAQSAGAAS
WHQGVEILPQAERLTRTLSKIHGDKGIDFELKLAAELVFKGDKGDLTELLGNLLDNACKAARSRVCLSAAKQDGRLRLIV
EDDGPGVDESMRDKIFERGIRADTYDKGHGIGLAIVQDLTDAYQGRLLLGRSDLLGGARFEVSFPQ
>Mature_465_residues
SHSASTPKPVPAAAPAHKQAAAFIDRLSLKTRLFVSAAILVALLLPALGIALFQAFERQALAGSRDELGALIYSVLAQTD
VIDGKLEMPPFLNEPQFNVDGSGLYAEVRSGAGEVLWRSGSLIGHGAIPALPSPAPGADAKVFGETLLNDEPLFVMSFTA
VYESKGVDVPLSIHILKSQQRYREALTEFESSLWRYLLLALALLAVVLGLWLRWTLKPLSRFEAELKAVEHGDKERIDTD
YPTELGPLVHQLNSLLTTEQNQRKRYRNALSDLAHSLKTPLAVLQSHPGLDDAVMEQVDNISHSISHQLKRAQSAGAASW
HQGVEILPQAERLTRTLSKIHGDKGIDFELKLAAELVFKGDKGDLTELLGNLLDNACKAARSRVCLSAAKQDGRLRLIVE
DDGPGVDESMRDKIFERGIRADTYDKGHGIGLAIVQDLTDAYQGRLLLGRSDLLGGARFEVSFPQ

Specific function: Member of the two-component regulatory system phoQ/phoP involved in virulence, adaptation to low Mg(2+) environments and the control of acid resistance genes. In presence of low periplasmic Mg(2+) concentrations, phoQ functions as a membrane- associated p

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787374, Length=284, Percent_Identity=38.7323943661972, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1788393, Length=274, Percent_Identity=28.1021897810219, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1790551, Length=265, Percent_Identity=28.6792452830189, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787894, Length=300, Percent_Identity=29.3333333333333, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR015014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF08918 PhoQ_Sensor [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50809; Mature: 50678

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSASTPKPVPAAAPAHKQAAAFIDRLSLKTRLFVSAAILVALLLPALGIALFQAFERQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAGSRDELGALIYSVLAQTDVIDGKLEMPPFLNEPQFNVDGSGLYAEVRSGAGEVLWRS
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEC
GSLIGHGAIPALPSPAPGADAKVFGETLLNDEPLFVMSFTAVYESKGVDVPLSIHILKSQ
CCEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCEEEHHHHHH
QRYREALTEFESSLWRYLLLALALLAVVLGLWLRWTLKPLSRFEAELKAVEHGDKERIDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
DYPTELGPLVHQLNSLLTTEQNQRKRYRNALSDLAHSLKTPLAVLQSHPGLDDAVMEQVD
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
NISHSISHQLKRAQSAGAASWHQGVEILPQAERLTRTLSKIHGDKGIDFELKLAAELVFK
HHHHHHHHHHHHHHHCCCHHHHHCHHHCCCHHHHHHHHHHHCCCCCCCEEHEEHHHHHCC
GDKGDLTELLGNLLDNACKAARSRVCLSAAKQDGRLRLIVEDDGPGVDESMRDKIFERGI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCC
RADTYDKGHGIGLAIVQDLTDAYQGRLLLGRSDLLGGARFEVSFPQ
CCCCCCCCCCCHHHHHHHHHHHHCCEEEECCHHCCCCCEEEECCCC
>Mature Secondary Structure 
SHSASTPKPVPAAAPAHKQAAAFIDRLSLKTRLFVSAAILVALLLPALGIALFQAFERQ
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAGSRDELGALIYSVLAQTDVIDGKLEMPPFLNEPQFNVDGSGLYAEVRSGAGEVLWRS
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEC
GSLIGHGAIPALPSPAPGADAKVFGETLLNDEPLFVMSFTAVYESKGVDVPLSIHILKSQ
CCEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCEEEHHHHHH
QRYREALTEFESSLWRYLLLALALLAVVLGLWLRWTLKPLSRFEAELKAVEHGDKERIDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
DYPTELGPLVHQLNSLLTTEQNQRKRYRNALSDLAHSLKTPLAVLQSHPGLDDAVMEQVD
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
NISHSISHQLKRAQSAGAASWHQGVEILPQAERLTRTLSKIHGDKGIDFELKLAAELVFK
HHHHHHHHHHHHHHHCCCHHHHHCHHHCCCHHHHHHHHHHHCCCCCCCEEHEEHHHHHCC
GDKGDLTELLGNLLDNACKAARSRVCLSAAKQDGRLRLIVEDDGPGVDESMRDKIFERGI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCC
RADTYDKGHGIGLAIVQDLTDAYQGRLLLGRSDLLGGARFEVSFPQ
CCCCCCCCCCCHHHHHHHHHHHHCCEEEECCHHCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]