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The map label for this gene is rlmI

Identifier: 119776097

GI number: 119776097

Start: 3529208

End: 3530398

Strand: Reverse

Name: rlmI

Synonym: Sama_2965

Alternate gene names: 119776097

Gene position: 3530398-3529208 (Counterclockwise)

Preceding gene: 119776098

Following gene: 119776096

Centisome position: 81.99

GC content: 59.7

Gene sequence:

>1191_bases
ATGAGCATTCGCATCAAACTCAAGCCGGGACGGGAAAAGTCCCTCGACCGTCGTCACCCCTGGGTGTTCTCCAGTGGCGT
ACACAACATCAAGGGCAAGCCCCTGCGCGGTGAAACCGTGGATGTAGTGGCCCACGATGGCCGCTGGCTTGGCCGCGGTG
CCTGGTCAGATGCCTCACAGATTCAGGTGCGTGTCTGGACCTTCGATAAAGAAGAGGCCATCGATCGTGACTTTTTCGCC
CGCCGTATCGCCCGTGCCCAGGCTGGCCGCGAGGCGCTTATCCGCGAAAAGGGCCTCACCGGCTATCGTCTGGTGGGCGC
CGAGTCTGACGGCCTGCCCGGTATCACCATCGACCGCTACGCCAATGTGCTGGTGTGTCAGTTGCTGTCTGCCGGTGCTG
ATTATTGGCGCGAGACCCTGGTATCAGTACTCGCCGAGCAGTTCCCGGAGTGTGCCATTTACGAGCGCTCTGACGTGGAC
TCACGTAAGAAAGAAGGCCTGCCACTGGTGACGGGTTTGCTCCACGGAGAGCTGCCTGCCATGCCGCTGATTATTGAAGA
AAACGGCATCAAGATTGCCGTCGATGTGGAAAAGGGCCACAAGACCGGCTTTTATCTCGACCAGCGCGACAACCGCGCCA
TGGCTGGGCGCTTTGTGGAAGGCAAGTCGGTACTCAACTGTTTCTGTTACACAGGCACCTTCGGCCTCTACGCCGCCAAG
GCCGGAGCCGCCAGTATCGAAAACGTGGACGTATCCACCCTGGCGCTGGATACTGCCCGTCACAACATGGCCATCAATGG
CCTTAACGACGACCATGTGACTTACAGCGAAGCCGATGTATTCAAGCTGTTGCGCCAGTACCGCGACGAAGGCAAAACCT
TCGATGTGATAGTGCTCGACCCGCCCAAGTTTGCCGATAACAAGGCCCAGCTCAATGGCGCCTGCCGTGGCTACAAGGAC
ATCAACATGATTGCCATGCAGCTATTGAAGCCCGGCGGCACCCTGCTGACCTTCTCCTGCTCCGGCCTGATGCCCGCCGA
CCTGTTCCAGAAAATTGTCGCCGATGCCGCACTGGATGCAGGCCGCGACGCCCAGTTTATCGAGCGCCTGAGCCAGGCCT
CGGACCACCCCATCGGCGCCGCCTTCCCGGAAGGTTTCTACCTCAAGGGGCTCGTTGCCCGGGTTTGGTAA

Upstream 100 bases:

>100_bases
TAGCCGCCATCCACGGCAGCGGCAGCCGCTAAACAGCAGTTGTGTCACCCGCCGCTCCCAATCTGTAACACGCCTTATTG
GGCGTAATCGAGACATCTTC

Downstream 100 bases:

>100_bases
CGCGAAGCACTATTGAAAAAGCCTGTTCATGATGAGCAGGCTTTTTTGTTTTTCGAGCCTTTAAAATGGCACGTTGCGGT
TACTCATCAAGCACGGGTTT

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSIRIKLKPGREKSLDRRHPWVFSSGVHNIKGKPLRGETVDVVAHDGRWLGRGAWSDASQIQVRVWTFDKEEAIDRDFFA
RRIARAQAGREALIREKGLTGYRLVGAESDGLPGITIDRYANVLVCQLLSAGADYWRETLVSVLAEQFPECAIYERSDVD
SRKKEGLPLVTGLLHGELPAMPLIIEENGIKIAVDVEKGHKTGFYLDQRDNRAMAGRFVEGKSVLNCFCYTGTFGLYAAK
AGAASIENVDVSTLALDTARHNMAINGLNDDHVTYSEADVFKLLRQYRDEGKTFDVIVLDPPKFADNKAQLNGACRGYKD
INMIAMQLLKPGGTLLTFSCSGLMPADLFQKIVADAALDAGRDAQFIERLSQASDHPIGAAFPEGFYLKGLVARVW

Sequences:

>Translated_396_residues
MSIRIKLKPGREKSLDRRHPWVFSSGVHNIKGKPLRGETVDVVAHDGRWLGRGAWSDASQIQVRVWTFDKEEAIDRDFFA
RRIARAQAGREALIREKGLTGYRLVGAESDGLPGITIDRYANVLVCQLLSAGADYWRETLVSVLAEQFPECAIYERSDVD
SRKKEGLPLVTGLLHGELPAMPLIIEENGIKIAVDVEKGHKTGFYLDQRDNRAMAGRFVEGKSVLNCFCYTGTFGLYAAK
AGAASIENVDVSTLALDTARHNMAINGLNDDHVTYSEADVFKLLRQYRDEGKTFDVIVLDPPKFADNKAQLNGACRGYKD
INMIAMQLLKPGGTLLTFSCSGLMPADLFQKIVADAALDAGRDAQFIERLSQASDHPIGAAFPEGFYLKGLVARVW
>Mature_395_residues
SIRIKLKPGREKSLDRRHPWVFSSGVHNIKGKPLRGETVDVVAHDGRWLGRGAWSDASQIQVRVWTFDKEEAIDRDFFAR
RIARAQAGREALIREKGLTGYRLVGAESDGLPGITIDRYANVLVCQLLSAGADYWRETLVSVLAEQFPECAIYERSDVDS
RKKEGLPLVTGLLHGELPAMPLIIEENGIKIAVDVEKGHKTGFYLDQRDNRAMAGRFVEGKSVLNCFCYTGTFGLYAAKA
GAASIENVDVSTLALDTARHNMAINGLNDDHVTYSEADVFKLLRQYRDEGKTFDVIVLDPPKFADNKAQLNGACRGYKDI
NMIAMQLLKPGGTLLTFSCSGLMPADLFQKIVADAALDAGRDAQFIERLSQASDHPIGAAFPEGFYLKGLVARVW

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Escherichia coli, GI145693111, Length=395, Percent_Identity=60.5063291139241, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1787181, Length=280, Percent_Identity=28.2142857142857, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMI_SHEAM (A1S9W1)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928837.1
- ProteinModelPortal:   A1S9W1
- SMR:   A1S9W1
- STRING:   A1S9W1
- GeneID:   4605212
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2965
- NMPDR:   fig|326297.7.peg.2781
- eggNOG:   COG1092
- HOGENOM:   HBG411897
- OMA:   RAAYSPH
- PhylomeDB:   A1S9W1
- ProtClustDB:   CLSK2462133
- GO:   GO:0005737
- HAMAP:   MF_01857
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614
- SMART:   SM00359

Pfam domain/function: PF10672 Methyltrans_SAM; SSF88697 PUA-like

EC number: =2.1.1.191

Molecular weight: Translated: 43765; Mature: 43634

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS50890 PUA; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRIKLKPGREKSLDRRHPWVFSSGVHNIKGKPLRGETVDVVAHDGRWLGRGAWSDASQ
CEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCE
IQVRVWTFDKEEAIDRDFFARRIARAQAGREALIREKGLTGYRLVGAESDGLPGITIDRY
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHHH
ANVLVCQLLSAGADYWRETLVSVLAEQFPECAIYERSDVDSRKKEGLPLVTGLLHGELPA
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCEEEHHHCCCCCC
MPLIIEENGIKIAVDVEKGHKTGFYLDQRDNRAMAGRFVEGKSVLNCFCYTGTFGLYAAK
CEEEEECCCEEEEEEECCCCCCCEEEECCCCCCHHCEEECCCHHEEEEEECCCCHHHHHC
AGAASIENVDVSTLALDTARHNMAINGLNDDHVTYSEADVFKLLRQYRDEGKTFDVIVLD
CCCCCCCCCCCHHEEEEHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEC
PPKFADNKAQLNGACRGYKDINMIAMQLLKPGGTLLTFSCSGLMPADLFQKIVADAALDA
CCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
GRDAQFIERLSQASDHPIGAAFPEGFYLKGLVARVW
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SIRIKLKPGREKSLDRRHPWVFSSGVHNIKGKPLRGETVDVVAHDGRWLGRGAWSDASQ
EEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCE
IQVRVWTFDKEEAIDRDFFARRIARAQAGREALIREKGLTGYRLVGAESDGLPGITIDRY
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHHH
ANVLVCQLLSAGADYWRETLVSVLAEQFPECAIYERSDVDSRKKEGLPLVTGLLHGELPA
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCEEEHHHCCCCCC
MPLIIEENGIKIAVDVEKGHKTGFYLDQRDNRAMAGRFVEGKSVLNCFCYTGTFGLYAAK
CEEEEECCCEEEEEEECCCCCCCEEEECCCCCCHHCEEECCCHHEEEEEECCCCHHHHHC
AGAASIENVDVSTLALDTARHNMAINGLNDDHVTYSEADVFKLLRQYRDEGKTFDVIVLD
CCCCCCCCCCCHHEEEEHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEC
PPKFADNKAQLNGACRGYKDINMIAMQLLKPGGTLLTFSCSGLMPADLFQKIVADAALDA
CCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
GRDAQFIERLSQASDHPIGAAFPEGFYLKGLVARVW
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA