Click here to switch to the map view.

The map label for this gene is dhfrIII [H]

Identifier: 119775956

GI number: 119775956

Start: 3363472

End: 3363954

Strand: Reverse

Name: dhfrIII [H]

Synonym: Sama_2824

Alternate gene names: 119775956

Gene position: 3363954-3363472 (Counterclockwise)

Preceding gene: 119775957

Following gene: 119775955

Centisome position: 78.12

GC content: 49.69

Gene sequence:

>483_bases
ATGCGTATCGCCATGATTGCCGCGATGGCCAATAACCGGGTGATAGGAAAAGATAACCAAATGCCCTGGCATTTGCCGGA
AGATCTCAAGCATTTCAAGGCTATGACGCTGGGCAAACCCGTGGTGATGGGCCGTAAAACCTTCGAATCCATAGGTCGTC
CATTGCCCGGGCGTCACAATATTGTGATTTCACGCCAGAGTGACTACTCCCACGAAGGTATTAGTCTGGTGTCTTCATTT
GAAGAAGCCGTGGCGCTGGCCGGTGATTGTGAGGAGTTGGTGGTTATCGGTGGTGGGCAACTCTATCAAACCCTGATTTC
CCAGGCAGATAAGCTATATTTAACCGAGATAGCCTTGGAGGTTGAGGGGGATACCCACTTCCCAGCCTGGGATGATGGCA
GCTGGGAAACGGTTGAATCCACTGAACATGTGTCAGACAAAGGCCTTCAATATCGCTTTATCACCTTGGTAAAAAAGTGT
TAA

Upstream 100 bases:

>100_bases
CTTTATCCTCGGTGCCTTGTCGGTGGGCCTGGCGCTGCCGAGTCTGCTGTATTTTCGTACCCGCCCCATCATATAAACAC
AACTCAAAGAGGGAGCTTTT

Downstream 100 bases:

>100_bases
ATTAATCGCCGTGGTACGTGCCCTAAAAGTAAATTCGAAAATACGATTTTACTGATAAATATAAAAAACAATCCGAGGAG
TATGAGATGCGTGTTTTGCC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRIAMIAAMANNRVIGKDNQMPWHLPEDLKHFKAMTLGKPVVMGRKTFESIGRPLPGRHNIVISRQSDYSHEGISLVSSF
EEAVALAGDCEELVVIGGGQLYQTLISQADKLYLTEIALEVEGDTHFPAWDDGSWETVESTEHVSDKGLQYRFITLVKKC

Sequences:

>Translated_160_residues
MRIAMIAAMANNRVIGKDNQMPWHLPEDLKHFKAMTLGKPVVMGRKTFESIGRPLPGRHNIVISRQSDYSHEGISLVSSF
EEAVALAGDCEELVVIGGGQLYQTLISQADKLYLTEIALEVEGDTHFPAWDDGSWETVESTEHVSDKGLQYRFITLVKKC
>Mature_160_residues
MRIAMIAAMANNRVIGKDNQMPWHLPEDLKHFKAMTLGKPVVMGRKTFESIGRPLPGRHNIVISRQSDYSHEGISLVSSF
EEAVALAGDCEELVVIGGGQLYQTLISQADKLYLTEIALEVEGDTHFPAWDDGSWETVESTEHVSDKGLQYRFITLVKKC

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=173, Percent_Identity=31.2138728323699, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1786233, Length=144, Percent_Identity=54.1666666666667, Blast_Score=161, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17505899, Length=179, Percent_Identity=31.8435754189944, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324810, Length=135, Percent_Identity=34.8148148148148, Blast_Score=68, Evalue=6e-13,
Organism=Drosophila melanogaster, GI116008026, Length=180, Percent_Identity=29.4444444444444, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24647458, Length=180, Percent_Identity=29.4444444444444, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17860; Mature: 17860

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAMIAAMANNRVIGKDNQMPWHLPEDLKHFKAMTLGKPVVMGRKTFESIGRPLPGRHN
CCEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHCCCCCCCCC
IVISRQSDYSHEGISLVSSFEEAVALAGDCEELVVIGGGQLYQTLISQADKLYLTEIALE
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCEEEEEEEEE
VEGDTHFPAWDDGSWETVESTEHVSDKGLQYRFITLVKKC
ECCCCCCCCCCCCCCCCCCCCHHHHCCCCEEHHHHHHHCC
>Mature Secondary Structure
MRIAMIAAMANNRVIGKDNQMPWHLPEDLKHFKAMTLGKPVVMGRKTFESIGRPLPGRHN
CCEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHCCCCCCCCC
IVISRQSDYSHEGISLVSSFEEAVALAGDCEELVVIGGGQLYQTLISQADKLYLTEIALE
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCEEEEEEEEE
VEGDTHFPAWDDGSWETVESTEHVSDKGLQYRFITLVKKC
ECCCCCCCCCCCCCCCCCCCCHHHHCCCCEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]