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The map label for this gene is phnE [H]

Identifier: 119774682

GI number: 119774682

Start: 1881075

End: 1882574

Strand: Direct

Name: phnE [H]

Synonym: Sama_1545

Alternate gene names: 119774682

Gene position: 1881075-1882574 (Clockwise)

Preceding gene: 119774681

Following gene: 119774685

Centisome position: 43.68

GC content: 53.33

Gene sequence:

>1500_bases
ATGCTGAACCTGGCGTTTATCGGCCGTTGGCAGCGGTTGACCCTGGGGTTATGGTTACTGTGTTTTTGCGCCTTTTGGCT
GGCAGATACCGAAGTGATTGCCCTTGAGCCCTGGAATGAACTGGGGCGTATGTTTGCAGGTTTTATAACGCCGGACTTTT
TTGCCACCGAGCATTTAATTGATGCGCTATGGCAAACGGTGAGCTTTACGCTGCTTGGGATAAGTCTTGGTCTGGTTATC
GGCGTGCCATTGTCGCTGGTGTACCATCAGCCACTTGTTGCCGCAGGTTGCGCTTTTATCCGCGCCATTCACGAGATTTT
CTGGGCATTGCTGTTTTTGCAACTGTTTGGCTTATCGCCGGTTACGGGAGTGTTGGCATTGGCGCTGCCCTACGGCACCA
CGTTTGCCCGGGTTTTTCACGATATTCTCTGTAGGGCGCCCAAAGACACTGCCGATTCCTTGCCTGGTGGAACTGATCCC
CTGAGCCAGTGGCTCTATGGCAAGATTGCCCATGTATTACCGGAGCTGCTTGCCTATACCCGTTATCGATTTGAATGCGC
GTTAAGAAGCAGTGCGGTGCTCGGATTTGTGGGCATGCCAACCCTTGGCTTTCATTTGGAGAGTGCTTTTAAACAGGGGA
ATTATTCGGAAGGCGCCGCGCTTTTGCTGTTGTTTATCTTCCTGATTGGGACCCTGCCTTACTGGTGCAAGCGTTGGCTG
CTCCCGTTTTATATGCTGGCAGCGGTATATTTTCTGCCACCGGTCGCAGGGCTTGATGGCAGTCTTATATGGCGTTTTAT
CAGCGAGGATATCTGGCCCCACGGTCTTAGACAGTGGCAACTCGGTGATTTGGGGTTAGGGGCAGCCCTGGTCGATACCC
TGGGCTGGCTTCAAGGATTAATCCTGTCTCAGGCATTGCCGGGTATTATGGCAACGCTTGTACTTGCTTTGGGGGCGCTG
GGGCTAACGCATTTGCTGGTGTGTTTTTTATTGCCCCTGGCTTATAAGCCCTTATCCGGCCGACTGGTGTCTGGCGCCAG
CACGCTGTCGCTGCTGTTGATGCGTTCTATACCGGAATACATCCTGGCCTTTGTGTTTATGCTGCTGCTTGGCCCGTCGA
TGTTGCCCGCGATGTTGGCTTTGGCGCTGCACAATGCGGGGCTGATTTTATTTTTGACCGCGAGAAGCGCCAACCGCATG
CCGCCGCGCGACGAAGATAAGGGCGGGCTCGATGGTTTTGGTTATCGGGTATTGCCTGCCATTTATCCGGGGATGATGGC
GCTGCTGTTTTATCGATTTGAAGTGATCCTGCGGGAAACGGCCATTCTCGGCATGTTGGGTGTGGCAACACTTGGTTTTT
ATATTGACAGTAACTTTTCTGAAATTCGTTTTGCCGGAGCCATGGTGCTGATGGGAATAACAGCCATGGTGAATGTGTTG
GTGGACGCCGCTGCCCGCCGGGCTCTGGGACAGGGGCGCCATAAAACTGAGCGATGCTGA

Upstream 100 bases:

>100_bases
TGAAAAATGGCCAACTGGTGTTGGATGCACCTGCTTCGACGTTGACCCTGGCCGACCTTGATGCTTTTTATGGTGATGCG
ATGAAGGATGAAGAGGCACA

Downstream 100 bases:

>100_bases
GGCTGGTAAAGGGAAACTGAAACAGGATTAAGGGATTAGGGGAGGGGAATGACACGTCAGGAAAAATCGTCACTCCCGTT
ACCGAATTGACACGTTAACC

Product: binding-protein-dependent transport systems inner membrane component

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MLNLAFIGRWQRLTLGLWLLCFCAFWLADTEVIALEPWNELGRMFAGFITPDFFATEHLIDALWQTVSFTLLGISLGLVI
GVPLSLVYHQPLVAAGCAFIRAIHEIFWALLFLQLFGLSPVTGVLALALPYGTTFARVFHDILCRAPKDTADSLPGGTDP
LSQWLYGKIAHVLPELLAYTRYRFECALRSSAVLGFVGMPTLGFHLESAFKQGNYSEGAALLLLFIFLIGTLPYWCKRWL
LPFYMLAAVYFLPPVAGLDGSLIWRFISEDIWPHGLRQWQLGDLGLGAALVDTLGWLQGLILSQALPGIMATLVLALGAL
GLTHLLVCFLLPLAYKPLSGRLVSGASTLSLLLMRSIPEYILAFVFMLLLGPSMLPAMLALALHNAGLILFLTARSANRM
PPRDEDKGGLDGFGYRVLPAIYPGMMALLFYRFEVILRETAILGMLGVATLGFYIDSNFSEIRFAGAMVLMGITAMVNVL
VDAAARRALGQGRHKTERC

Sequences:

>Translated_499_residues
MLNLAFIGRWQRLTLGLWLLCFCAFWLADTEVIALEPWNELGRMFAGFITPDFFATEHLIDALWQTVSFTLLGISLGLVI
GVPLSLVYHQPLVAAGCAFIRAIHEIFWALLFLQLFGLSPVTGVLALALPYGTTFARVFHDILCRAPKDTADSLPGGTDP
LSQWLYGKIAHVLPELLAYTRYRFECALRSSAVLGFVGMPTLGFHLESAFKQGNYSEGAALLLLFIFLIGTLPYWCKRWL
LPFYMLAAVYFLPPVAGLDGSLIWRFISEDIWPHGLRQWQLGDLGLGAALVDTLGWLQGLILSQALPGIMATLVLALGAL
GLTHLLVCFLLPLAYKPLSGRLVSGASTLSLLLMRSIPEYILAFVFMLLLGPSMLPAMLALALHNAGLILFLTARSANRM
PPRDEDKGGLDGFGYRVLPAIYPGMMALLFYRFEVILRETAILGMLGVATLGFYIDSNFSEIRFAGAMVLMGITAMVNVL
VDAAARRALGQGRHKTERC
>Mature_499_residues
MLNLAFIGRWQRLTLGLWLLCFCAFWLADTEVIALEPWNELGRMFAGFITPDFFATEHLIDALWQTVSFTLLGISLGLVI
GVPLSLVYHQPLVAAGCAFIRAIHEIFWALLFLQLFGLSPVTGVLALALPYGTTFARVFHDILCRAPKDTADSLPGGTDP
LSQWLYGKIAHVLPELLAYTRYRFECALRSSAVLGFVGMPTLGFHLESAFKQGNYSEGAALLLLFIFLIGTLPYWCKRWL
LPFYMLAAVYFLPPVAGLDGSLIWRFISEDIWPHGLRQWQLGDLGLGAALVDTLGWLQGLILSQALPGIMATLVLALGAL
GLTHLLVCFLLPLAYKPLSGRLVSGASTLSLLLMRSIPEYILAFVFMLLLGPSMLPAMLALALHNAGLILFLTARSANRM
PPRDEDKGGLDGFGYRVLPAIYPGMMALLFYRFEVILRETAILGMLGVATLGFYIDSNFSEIRFAGAMVLMGITAMVNVL
VDAAARRALGQGRHKTERC

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 54894; Mature: 54894

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA