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The map label for this gene is mak [H]

Identifier: 119774542

GI number: 119774542

Start: 1706682

End: 1707614

Strand: Direct

Name: mak [H]

Synonym: Sama_1405

Alternate gene names: 119774542

Gene position: 1706682-1707614 (Clockwise)

Preceding gene: 119774541

Following gene: 119774543

Centisome position: 39.63

GC content: 57.88

Gene sequence:

>933_bases
ATGTATCGCTCTGGGATTGATTTGGGCGGCACCAAGATTGAATTGGTGACCTTGAATGAAAAGGGGGAGGAGGTATTTCG
CAAGCGTGTACCTACGCCCAAGGACTACCGTGCGACCCTCGAGGCGGTTGCTGGCCTGGTGCATGACTCTGAAAAGGAAA
CCGGACAGGTGTCCAGTGTCGGGATTGGTATTCCCGGTGTGGTGTCTGCGGTCACCGGCAGGGTAAAGAACTCCAATGCC
GTGTGGCTGAATGGACAGCCGATGGATAAGGATCTCGGTGCCATGCTGGGCCGGGAGGTGCGTATTGCCAACGATGCCAA
TTGCTTTGCAGTGTCTGAGTCTGTGGATGGCGGCGGCGCCGGTAAAACCCTGGTATTTGGTGCCATTCTGGGCACCGGCT
GCGGCGCAGGTATTGCGATTAACCACAAGGTACATGGCGGTGGTAACGGCATTGGCGGTGAATGGGGCCATAACCCGCTG
CCGTGGATGACGGCAGATGAGTTTAACTCCACCCGCTGCTTCTGCGGCAACGCCGATTGCATCGAAACCTTTGTTTCGGG
CACAGGCTTTGTGCGGGACTTTCGTGCCCATGGTGGCGAGGCGGCAAGCGGTATTGAAATTGTGGCGCTGATGGGCAAGG
GCGAACCCTTGGCTGAAGCCGCCTTTGGCCGCTTTATCGACCGTTTGGCCAGAGCGCTGGCGCACGTTATCAATCTGCTC
GACCCGGACGTGATAGTCCTTGGCGGCGGTGTGTCCAATATCGATGAGATTTACGAGTATCTGCCCGCCTTGCTGCCCAA
GTACGTGTTGGGCGGCGAGTGTGCCACCAAGGTGGTGAAAAACCATCATGGAGCGTCCTCAGGGGTACGGGGTGCAGCTT
GGCTGTGGGCACCCGGTGAACAGGCTGCCTTGCCTGGTCTGCAGGAATTGTAA

Upstream 100 bases:

>100_bases
TGGAATGTGGATTGCCGCCGGTCAGCGACAAATGACAGCACTGAGCATGACCGCGACATGCCGCGCTGAGATGAAAAACA
CCATCAACATGGAGAATGAG

Downstream 100 bases:

>100_bases
TTAAGGGGGCTGTTAACTCCGAATAGGTGGGCAGTGTTGGTGCTGCCACATGAAATAGGTTTGTTGCTTATCCCTCATGG
ATGAGGGCTTTTATGTGCTT

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MYRSGIDLGGTKIELVTLNEKGEEVFRKRVPTPKDYRATLEAVAGLVHDSEKETGQVSSVGIGIPGVVSAVTGRVKNSNA
VWLNGQPMDKDLGAMLGREVRIANDANCFAVSESVDGGGAGKTLVFGAILGTGCGAGIAINHKVHGGGNGIGGEWGHNPL
PWMTADEFNSTRCFCGNADCIETFVSGTGFVRDFRAHGGEAASGIEIVALMGKGEPLAEAAFGRFIDRLARALAHVINLL
DPDVIVLGGGVSNIDEIYEYLPALLPKYVLGGECATKVVKNHHGASSGVRGAAWLWAPGEQAALPGLQEL

Sequences:

>Translated_310_residues
MYRSGIDLGGTKIELVTLNEKGEEVFRKRVPTPKDYRATLEAVAGLVHDSEKETGQVSSVGIGIPGVVSAVTGRVKNSNA
VWLNGQPMDKDLGAMLGREVRIANDANCFAVSESVDGGGAGKTLVFGAILGTGCGAGIAINHKVHGGGNGIGGEWGHNPL
PWMTADEFNSTRCFCGNADCIETFVSGTGFVRDFRAHGGEAASGIEIVALMGKGEPLAEAAFGRFIDRLARALAHVINLL
DPDVIVLGGGVSNIDEIYEYLPALLPKYVLGGECATKVVKNHHGASSGVRGAAWLWAPGEQAALPGLQEL
>Mature_310_residues
MYRSGIDLGGTKIELVTLNEKGEEVFRKRVPTPKDYRATLEAVAGLVHDSEKETGQVSSVGIGIPGVVSAVTGRVKNSNA
VWLNGQPMDKDLGAMLGREVRIANDANCFAVSESVDGGGAGKTLVFGAILGTGCGAGIAINHKVHGGGNGIGGEWGHNPL
PWMTADEFNSTRCFCGNADCIETFVSGTGFVRDFRAHGGEAASGIEIVALMGKGEPLAEAAFGRFIDRLARALAHVINLL
DPDVIVLGGGVSNIDEIYEYLPALLPKYVLGGECATKVVKNHHGASSGVRGAAWLWAPGEQAALPGLQEL

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=298, Percent_Identity=58.7248322147651, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1787363, Length=300, Percent_Identity=40.3333333333333, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1790522, Length=309, Percent_Identity=27.5080906148867, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786891, Length=274, Percent_Identity=21.8978102189781, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32319; Mature: 32319

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRSGIDLGGTKIELVTLNEKGEEVFRKRVPTPKDYRATLEAVAGLVHDSEKETGQVSSV
CCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEE
GIGIPGVVSAVTGRVKNSNAVWLNGQPMDKDLGAMLGREVRIANDANCFAVSESVDGGGA
CCCCHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHCCEEEEECCCCEEEEECCCCCCCC
GKTLVFGAILGTGCGAGIAINHKVHGGGNGIGGEWGHNPLPWMTADEFNSTRCFCGNADC
CHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHH
IETFVSGTGFVRDFRAHGGEAASGIEIVALMGKGEPLAEAAFGRFIDRLARALAHVINLL
HHHHHCCCCHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHC
DPDVIVLGGGVSNIDEIYEYLPALLPKYVLGGECATKVVKNHHGASSGVRGAAWLWAPGE
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEECCCC
QAALPGLQEL
HHHCCCHHCC
>Mature Secondary Structure
MYRSGIDLGGTKIELVTLNEKGEEVFRKRVPTPKDYRATLEAVAGLVHDSEKETGQVSSV
CCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEE
GIGIPGVVSAVTGRVKNSNAVWLNGQPMDKDLGAMLGREVRIANDANCFAVSESVDGGGA
CCCCHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHCCEEEEECCCCEEEEECCCCCCCC
GKTLVFGAILGTGCGAGIAINHKVHGGGNGIGGEWGHNPLPWMTADEFNSTRCFCGNADC
CHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHH
IETFVSGTGFVRDFRAHGGEAASGIEIVALMGKGEPLAEAAFGRFIDRLARALAHVINLL
HHHHHCCCCHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHC
DPDVIVLGGGVSNIDEIYEYLPALLPKYVLGGECATKVVKNHHGASSGVRGAAWLWAPGE
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEECCCC
QAALPGLQEL
HHHCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]