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The map label for this gene is paaF [C]

Identifier: 119774516

GI number: 119774516

Start: 1670638

End: 1671744

Strand: Direct

Name: paaF [C]

Synonym: Sama_1379

Alternate gene names: 119774516

Gene position: 1670638-1671744 (Clockwise)

Preceding gene: 119774515

Following gene: 119774517

Centisome position: 38.8

GC content: 56.91

Gene sequence:

>1107_bases
ATGACACAGAAAGTTGTATTTCAAACCCTGGGGACCGCCTCGGGCAAGCAGATAGGTGTGGCGACGCTCAATGTGGAAGC
CGCACTCAATGCGCTGGATTTGGATATGGTGCGGGCGCTTTCTGCCCAGCTGAAAGCCTGGCAGGCCGATAGTAATATCG
CCATGGTGCTGCTCGATGGCGCCGGAGACAAAGCCTTTTGTGCCGGTGGCGATGTGCGAGCCCTGTATCAGGCCAGCAAA
GACGCTCCCGGTAGCACTGAGACCCTGGCAAAAACCTTTTTCGAGGAAGAATATCGACTCGATTACTTTATTCATGAATT
CGGCAAGCCATTTATGGTGTGGGGTGACGGCATTGTAATGGGGGGCGGTTTGGGCCTGATGGCCGGTGCCAGCCACCGCA
TTGCCACCGAGCGTTCCCGCATTGCCATGCCGGAAATCACCATTGGCTTGTATCCGGATGTGGGCGGCACCTTCTTTTTG
GCCCATATGCCGGAAAAGACAGGTGTCTTCCTGGGCTTAACCGCATTTCAAATGAATGGCGCCGATGCCCTCTATGCAGG
TACCGCCAACTTCTTATTGGAAAGTGATGATAAGGAGCCGCTGCTCGATGCCCTGGCCGAGGTCGCCTGGGATGACCACA
GCGAAGCCAATCATCAGCGTCTGAGTGACGTGCTCGGCACCAGAGCCGTGCCTGAGCAGGCGAGCTTCCTTAAAGACAAT
GCTGAGCTTATCAGTGAGCTGTGCAGTGGCGATTTGGACACTGTGATTGAGCGTTTCAAAGCGTTGCCCGACGACAGCCA
CAAATCCCTGCTAAGAGCCCGCGACACCCTGCTGGCCGGCAGTCCACTCAGTGCCCATCTGGTATGGCACCAGGCCATCA
TAGGTGAAGAACTGTCGCTGAACGATTGCTTCCGCTGGGAGCTTGGCGTCAGCATCAACTGCTGCGCCCACGGCGATTTT
GTCGAAGGGGTGCGGGCACTGCTTATCGATAAAGACAGAAACCCAACCTGGCAGTACGCCGATGTGGCCTCGGTACCCGC
CGAGGCGATTGGCAGCCTTTTGAGTTCACCCTGGCAGGGCGAGCTGCATCCGCTGCAGGACCTCTGA

Upstream 100 bases:

>100_bases
ATCAAGCCGAAGGGGTGAATGCCTTCCTCGAGAAGCGTGCCGCCCAGTGGAAAAACCGCTGAGGCCTTGCCTTGGACATG
ACATTACGCAAGGAGCGAGC

Downstream 100 bases:

>100_bases
TAGATACCCTATTCACAGCCCGGCACATACCGGGCTTATCCGATTTTTGGCCCGGCCAGCCGGGCTTGGCAAGGAGCGAC
ATCATGGCGAAGATAGCGTT

Product: enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTQKVVFQTLGTASGKQIGVATLNVEAALNALDLDMVRALSAQLKAWQADSNIAMVLLDGAGDKAFCAGGDVRALYQASK
DAPGSTETLAKTFFEEEYRLDYFIHEFGKPFMVWGDGIVMGGGLGLMAGASHRIATERSRIAMPEITIGLYPDVGGTFFL
AHMPEKTGVFLGLTAFQMNGADALYAGTANFLLESDDKEPLLDALAEVAWDDHSEANHQRLSDVLGTRAVPEQASFLKDN
AELISELCSGDLDTVIERFKALPDDSHKSLLRARDTLLAGSPLSAHLVWHQAIIGEELSLNDCFRWELGVSINCCAHGDF
VEGVRALLIDKDRNPTWQYADVASVPAEAIGSLLSSPWQGELHPLQDL

Sequences:

>Translated_368_residues
MTQKVVFQTLGTASGKQIGVATLNVEAALNALDLDMVRALSAQLKAWQADSNIAMVLLDGAGDKAFCAGGDVRALYQASK
DAPGSTETLAKTFFEEEYRLDYFIHEFGKPFMVWGDGIVMGGGLGLMAGASHRIATERSRIAMPEITIGLYPDVGGTFFL
AHMPEKTGVFLGLTAFQMNGADALYAGTANFLLESDDKEPLLDALAEVAWDDHSEANHQRLSDVLGTRAVPEQASFLKDN
AELISELCSGDLDTVIERFKALPDDSHKSLLRARDTLLAGSPLSAHLVWHQAIIGEELSLNDCFRWELGVSINCCAHGDF
VEGVRALLIDKDRNPTWQYADVASVPAEAIGSLLSSPWQGELHPLQDL
>Mature_367_residues
TQKVVFQTLGTASGKQIGVATLNVEAALNALDLDMVRALSAQLKAWQADSNIAMVLLDGAGDKAFCAGGDVRALYQASKD
APGSTETLAKTFFEEEYRLDYFIHEFGKPFMVWGDGIVMGGGLGLMAGASHRIATERSRIAMPEITIGLYPDVGGTFFLA
HMPEKTGVFLGLTAFQMNGADALYAGTANFLLESDDKEPLLDALAEVAWDDHSEANHQRLSDVLGTRAVPEQASFLKDNA
ELISELCSGDLDTVIERFKALPDDSHKSLLRARDTLLAGSPLSAHLVWHQAIIGEELSLNDCFRWELGVSINCCAHGDFV
EGVRALLIDKDRNPTWQYADVASVPAEAIGSLLSSPWQGELHPLQDL

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=349, Percent_Identity=35.243553008596, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI37594469, Length=307, Percent_Identity=33.8762214983713, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI194097323, Length=176, Percent_Identity=30.1136363636364, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=142, Percent_Identity=30.9859154929577, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=337, Percent_Identity=37.6854599406528, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI25144160, Length=337, Percent_Identity=37.6854599406528, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI25145438, Length=151, Percent_Identity=30.4635761589404, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=343, Percent_Identity=27.6967930029155, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28571730, Length=342, Percent_Identity=37.719298245614, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI28571729, Length=342, Percent_Identity=37.719298245614, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI20129971, Length=185, Percent_Identity=28.6486486486486, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24653477, Length=185, Percent_Identity=28.6486486486486, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 39796; Mature: 39665

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKVVFQTLGTASGKQIGVATLNVEAALNALDLDMVRALSAQLKAWQADSNIAMVLLDG
CCHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEEC
AGDKAFCAGGDVRALYQASKDAPGSTETLAKTFFEEEYRLDYFIHEFGKPFMVWGDGIVM
CCCCEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEE
GGGLGLMAGASHRIATERSRIAMPEITIGLYPDVGGTFFLAHMPEKTGVFLGLTAFQMNG
ECCCHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEEEEEEECC
ADALYAGTANFLLESDDKEPLLDALAEVAWDDHSEANHQRLSDVLGTRAVPEQASFLKDN
CCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
AELISELCSGDLDTVIERFKALPDDSHKSLLRARDTLLAGSPLSAHLVWHQAIIGEELSL
HHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
NDCFRWELGVSINCCAHGDFVEGVRALLIDKDRNPTWQYADVASVPAEAIGSLLSSPWQG
CCCEEEEECCEEEEEECCCHHHHHHHHEEECCCCCCCCCCCHHHCCHHHHHHHHCCCCCC
ELHPLQDL
CCCCCCCC
>Mature Secondary Structure 
TQKVVFQTLGTASGKQIGVATLNVEAALNALDLDMVRALSAQLKAWQADSNIAMVLLDG
CHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEEC
AGDKAFCAGGDVRALYQASKDAPGSTETLAKTFFEEEYRLDYFIHEFGKPFMVWGDGIVM
CCCCEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEE
GGGLGLMAGASHRIATERSRIAMPEITIGLYPDVGGTFFLAHMPEKTGVFLGLTAFQMNG
ECCCHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEEEEEEECC
ADALYAGTANFLLESDDKEPLLDALAEVAWDDHSEANHQRLSDVLGTRAVPEQASFLKDN
CCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
AELISELCSGDLDTVIERFKALPDDSHKSLLRARDTLLAGSPLSAHLVWHQAIIGEELSL
HHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
NDCFRWELGVSINCCAHGDFVEGVRALLIDKDRNPTWQYADVASVPAEAIGSLLSSPWQG
CCCEEEEECCEEEEEECCCHHHHHHHHEEECCCCCCCCCCCHHHCCHHHHHHHHCCCCCC
ELHPLQDL
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA