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The map label for this gene is dosC [H]

Identifier: 119774199

GI number: 119774199

Start: 1286707

End: 1288023

Strand: Reverse

Name: dosC [H]

Synonym: Sama_1062

Alternate gene names: 119774199

Gene position: 1288023-1286707 (Counterclockwise)

Preceding gene: 119774200

Following gene: 119774198

Centisome position: 29.91

GC content: 52.92

Gene sequence:

>1317_bases
ATGGCCTCTGTCGCGCTTTTAGCCAGTGCAAGAGCTGGATTTCATCCGTATAATGGTCGGGCCAGAGATTCGATATGGAT
ATTTTTAGTGAAGCAACGGGACAGTCTCAAACACAAGCGAATTGATACCCTCGGCCTGCGTTACCACCTGATGTTATTGC
TGACGCCCCTGTCAGTGTTCGTCGGGATCTTTTACCTGTTTTCCCCCGCAGGCTCCCTGCCGTGGATGATTTTATTACTG
GTGTGCCTGGTGTATCTGATGGGATACAGCCTCAGCTATTACCTTCATCAGGGCCGACTGCAACGCTTGTGGGATCACCT
GGAGCAGGTGGTGTCCATTAACGAAGCCAGCATGGAGCTCATCCACCTCTCCAGCCAATACCAGAATGAACACGCCTTCC
TGGATGCCTTGCTCAATAAGGCGGTTTCGGTCATCAACGGCGCCGAGATGGGCAGTATTATCCGGGTCGATCCCGATACC
CATAAACTCATGTTTGAATCCGCTGTAGGCCTGGATCTGTCGCGGCTCAAAACCATAGATTTCTCTCTGGAGCAGTCTTT
TGAATATCGAATGACCAAGGGGCGCTGCGATAAGGTGGTGGTCATCGATGATATGCAGCATATCAATGCCCAAAGCACCC
TGACCCCGGCCGAGCAGCACATTTTGCTTACCGCCGCCAAACAGCCTATCCGCTCTACCCTCTCAAGCCCCATCCATATC
GACGGCAAACTCTACGCCATGCTGAATCTCGACAGCAGTCATCTGGCGGCTTTCAGCGATTATGATCGTAATCTGGTGGC
AATTTTGACCCACGAGGCCGCGAGCGCTATCGCCTTGTATCAAAAGTCGCGGGAAATTCAGCGCCTGGCCAATTTCGACA
TCCTCACAGGTCTCCATAACAGGCAGTATTTTGAGTCACAAGTCAGCAAGTGGCCCAAAAGCCGTCTCGGCAGTTATCTG
ACGGTACTGGATTTGGACAATCTCAAGGTACTCAACGACAGCCAAGGCCATCAGGCCGGTGACGAGGCGCTGGCAAAATT
GGCCGATGCCTTAAAAATGATCTGGCCGCAAAACAGCCTTATCAGCCGTTTTGGCGGTGATGAGTTCGTCATACTCACCC
AGGGCAGTGAAGAACAACTGCTGGAGCGATTGGCGCAGCTGAAGCGCACCATGGCCAGCACTGAGATGCAGGTGCTCTTC
AGTGCCGGGCTAGCCCCTTTCAATGGTGACTTTGCCGCCAGCTTCAAACTGGCCGATGAGCGTATGTACGCGCAAAAGCG
CAGCAGACACAAAGTAGCAGGTAAAGCACCAGCCTGA

Upstream 100 bases:

>100_bases
GGATGCCGACACAATATCAGGCACCACTTCAGTGGATCCTCGATAACGCCGACTTCTAAGCAAAGTCATCATCAAAGCCC
CGAGAATTCGGGGCTTTTTT

Downstream 100 bases:

>100_bases
ACCTGACTTACCCGGGAATTACAACAAGGACGTGTAGATGTATCCAATCCTCAGGGCAAGCCTGCTTGCCAGCAGCCTGC
TATTTTCAATCTTCCCGACA

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MASVALLASARAGFHPYNGRARDSIWIFLVKQRDSLKHKRIDTLGLRYHLMLLLTPLSVFVGIFYLFSPAGSLPWMILLL
VCLVYLMGYSLSYYLHQGRLQRLWDHLEQVVSINEASMELIHLSSQYQNEHAFLDALLNKAVSVINGAEMGSIIRVDPDT
HKLMFESAVGLDLSRLKTIDFSLEQSFEYRMTKGRCDKVVVIDDMQHINAQSTLTPAEQHILLTAAKQPIRSTLSSPIHI
DGKLYAMLNLDSSHLAAFSDYDRNLVAILTHEAASAIALYQKSREIQRLANFDILTGLHNRQYFESQVSKWPKSRLGSYL
TVLDLDNLKVLNDSQGHQAGDEALAKLADALKMIWPQNSLISRFGGDEFVILTQGSEEQLLERLAQLKRTMASTEMQVLF
SAGLAPFNGDFAASFKLADERMYAQKRSRHKVAGKAPA

Sequences:

>Translated_438_residues
MASVALLASARAGFHPYNGRARDSIWIFLVKQRDSLKHKRIDTLGLRYHLMLLLTPLSVFVGIFYLFSPAGSLPWMILLL
VCLVYLMGYSLSYYLHQGRLQRLWDHLEQVVSINEASMELIHLSSQYQNEHAFLDALLNKAVSVINGAEMGSIIRVDPDT
HKLMFESAVGLDLSRLKTIDFSLEQSFEYRMTKGRCDKVVVIDDMQHINAQSTLTPAEQHILLTAAKQPIRSTLSSPIHI
DGKLYAMLNLDSSHLAAFSDYDRNLVAILTHEAASAIALYQKSREIQRLANFDILTGLHNRQYFESQVSKWPKSRLGSYL
TVLDLDNLKVLNDSQGHQAGDEALAKLADALKMIWPQNSLISRFGGDEFVILTQGSEEQLLERLAQLKRTMASTEMQVLF
SAGLAPFNGDFAASFKLADERMYAQKRSRHKVAGKAPA
>Mature_437_residues
ASVALLASARAGFHPYNGRARDSIWIFLVKQRDSLKHKRIDTLGLRYHLMLLLTPLSVFVGIFYLFSPAGSLPWMILLLV
CLVYLMGYSLSYYLHQGRLQRLWDHLEQVVSINEASMELIHLSSQYQNEHAFLDALLNKAVSVINGAEMGSIIRVDPDTH
KLMFESAVGLDLSRLKTIDFSLEQSFEYRMTKGRCDKVVVIDDMQHINAQSTLTPAEQHILLTAAKQPIRSTLSSPIHID
GKLYAMLNLDSSHLAAFSDYDRNLVAILTHEAASAIALYQKSREIQRLANFDILTGLHNRQYFESQVSKWPKSRLGSYLT
VLDLDNLKVLNDSQGHQAGDEALAKLADALKMIWPQNSLISRFGGDEFVILTQGSEEQLLERLAQLKRTMASTEMQVLFS
AGLAPFNGDFAASFKLADERMYAQKRSRHKVAGKAPA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=33.5443037974684, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=28.8135593220339, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87081974, Length=148, Percent_Identity=32.4324324324324, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081977, Length=230, Percent_Identity=29.5652173913043, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787056, Length=172, Percent_Identity=34.3023255813954, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787541, Length=152, Percent_Identity=31.5789473684211, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 49256; Mature: 49125

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVALLASARAGFHPYNGRARDSIWIFLVKQRDSLKHKRIDTLGLRYHLMLLLTPLSVF
CCCHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VGIFYLFSPAGSLPWMILLLVCLVYLMGYSLSYYLHQGRLQRLWDHLEQVVSINEASMEL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IHLSSQYQNEHAFLDALLNKAVSVINGAEMGSIIRVDPDTHKLMFESAVGLDLSRLKTID
HHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHH
FSLEQSFEYRMTKGRCDKVVVIDDMQHINAQSTLTPAEQHILLTAAKQPIRSTLSSPIHI
HHHHHHHHHHCCCCCCCEEEEECCHHHCCCCCCCCCCHHHEEEHHHHHHHHHHHCCCEEE
DGKLYAMLNLDSSHLAAFSDYDRNLVAILTHEAASAIALYQKSREIQRLANFDILTGLHN
CCEEEEEEECCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
RQYFESQVSKWPKSRLGSYLTVLDLDNLKVLNDSQGHQAGDEALAKLADALKMIWPQNSL
HHHHHHHHHHCCHHHHCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHH
ISRFGGDEFVILTQGSEEQLLERLAQLKRTMASTEMQVLFSAGLAPFNGDFAASFKLADE
HHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHH
RMYAQKRSRHKVAGKAPA
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ASVALLASARAGFHPYNGRARDSIWIFLVKQRDSLKHKRIDTLGLRYHLMLLLTPLSVF
CCHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VGIFYLFSPAGSLPWMILLLVCLVYLMGYSLSYYLHQGRLQRLWDHLEQVVSINEASMEL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IHLSSQYQNEHAFLDALLNKAVSVINGAEMGSIIRVDPDTHKLMFESAVGLDLSRLKTID
HHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHH
FSLEQSFEYRMTKGRCDKVVVIDDMQHINAQSTLTPAEQHILLTAAKQPIRSTLSSPIHI
HHHHHHHHHHCCCCCCCEEEEECCHHHCCCCCCCCCCHHHEEEHHHHHHHHHHHCCCEEE
DGKLYAMLNLDSSHLAAFSDYDRNLVAILTHEAASAIALYQKSREIQRLANFDILTGLHN
CCEEEEEEECCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
RQYFESQVSKWPKSRLGSYLTVLDLDNLKVLNDSQGHQAGDEALAKLADALKMIWPQNSL
HHHHHHHHHHCCHHHHCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHH
ISRFGGDEFVILTQGSEEQLLERLAQLKRTMASTEMQVLFSAGLAPFNGDFAASFKLADE
HHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHH
RMYAQKRSRHKVAGKAPA
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]