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The map label for this gene is gltD [H]

Identifier: 119773993

GI number: 119773993

Start: 1041950

End: 1043368

Strand: Reverse

Name: gltD [H]

Synonym: Sama_0856

Alternate gene names: 119773993

Gene position: 1043368-1041950 (Counterclockwise)

Preceding gene: 119773994

Following gene: 119773992

Centisome position: 24.23

GC content: 58.91

Gene sequence:

>1419_bases
ATGAGTAACGACTTTCAGTTTATTCAGGTAGGCCGCAAAGATCCGACCAAGCAGCCCATTAAGCTGCGTGCAACACAGTT
TATTGAAATTTATCAGCCTTTTACTCAACCACAGGTCAACGAGCAGGCAGACCGCTGCCTGGACTGCGGTAACCCTTACT
GTGAATGGAAGTGCCCGCTGCACAATTACATTCCCAACTGGCTGAAGCTCGCCAAACAGGGGCGCATCATGGAAGCGGCA
GATCTGGTCCACGAGACCAACACCCTGCCGGAAATCTGCGGCCGGGTTTGCCCCCAGGACAGGCTCTGTGAAGGCGCCTG
TACCCTGAATGATGATTTTGGCGCCGTAACCATAGGTAACGTCGAGAAATACATCACAGATACTGCCCTCGCCCAGGGTT
GGCGCCCTGATATGAGTAAGGTCACGCCCCGCAAAGAGCGGGTGGCGATCGTCGGTGCTGGCCCGGCGGGCCTTGGCTGC
GCCGATATCCTGGCCCGCAACGGCATCAAGGCAGTGGTGTTCGACAAATATCCACAAATCGGCGGCCTGCTGACCTACGG
TATTCCTTCATTCAAACTGGATAAGTCGGTGATGCAGACCCGCCGAACCGTGCTGGAGGGTATGGGCATCGAGTTTCGTC
TGGGCGTTACCGTGGGCAAGGACATCGCCTTCGCCGACCTGCTTAAAGACTATGACGCCGTATTCCTCGGCATGGGCACC
TACAGCGCCATGAAGGCGGGGCTGCCCGGTGAAGACGCCGACGGTGTGCTACAGGCATTGCCCTATCTGATTGGCAACAC
CATGTCGCTGCTCGGTCAGCAAAGTGAACAGAACCCCTATATCGACCTGGCAGGTAAAAAAGTCGTGGTACTGGGTGGCG
GTGACACCGCCATGGACTGCGTGCGTACCGCAGTGCGCCAGGGCGCGCGCGAAGTGACCTGTGCTTACCGCCGCGATGAA
GCCAACATGCCCGGCTCCCGCCGCGAGGTGCAAAACGCCCGCGAAGAAGGGGTGAACTTCCTGTTCAACCGTCAGCCTGT
TGCCATCAAGACCGAAAACGGCGCCGTGGTGGGTATCGAGTGCATCGAGACCCGCATGGGTGAACCCGATGCCAATGGCC
GCCGCTCTGCCGAGCCCGTTCCCGGCAGCGAACAGCTCCTCGAAGCCGATGCCATCATCATCGCCTTTGGCTTCCAGCCA
AGTCCTGCCCCCTGGTTTGCCGATTTCGATATCGCCCTGGATGACAAGGGCCGGGTCAAGGCCAGCAAAGCCGATGACAA
TCCATTCCAAACCAGCAATCCCAAGGTGTTTGCCGGTGGCGACATGGTCCGGGGCTCAGACCTGGTGGTCACTGCCATCG
CCGAAGGCCGCGAAGCCGCGCTCGGGATCCTCAACTATCTGGAATCGGCCAAAGCCTGA

Upstream 100 bases:

>100_bases
TGCTTCGTGCTGGTCAAGCCGAAGAATGTAGCGGTGGCCGATCTGCTCAAACTCGAGCAGCCAAGCCCTGAACTGGCAGT
AAAGGCGGGGTAACAGCAAG

Downstream 100 bases:

>100_bases
TCCCAACAACAAGTTGCTTGTGTCTTGCGCATCCAAAGGTTCGCCTGAGGATGCGCTTTTTTATCTCTGCAGCTTGCCTG
TGTGCCGGTCAGTCGACTGT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSNDFQFIQVGRKDPTKQPIKLRATQFIEIYQPFTQPQVNEQADRCLDCGNPYCEWKCPLHNYIPNWLKLAKQGRIMEAA
DLVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGNVEKYITDTALAQGWRPDMSKVTPRKERVAIVGAGPAGLGC
ADILARNGIKAVVFDKYPQIGGLLTYGIPSFKLDKSVMQTRRTVLEGMGIEFRLGVTVGKDIAFADLLKDYDAVFLGMGT
YSAMKAGLPGEDADGVLQALPYLIGNTMSLLGQQSEQNPYIDLAGKKVVVLGGGDTAMDCVRTAVRQGAREVTCAYRRDE
ANMPGSRREVQNAREEGVNFLFNRQPVAIKTENGAVVGIECIETRMGEPDANGRRSAEPVPGSEQLLEADAIIIAFGFQP
SPAPWFADFDIALDDKGRVKASKADDNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGREAALGILNYLESAKA

Sequences:

>Translated_472_residues
MSNDFQFIQVGRKDPTKQPIKLRATQFIEIYQPFTQPQVNEQADRCLDCGNPYCEWKCPLHNYIPNWLKLAKQGRIMEAA
DLVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGNVEKYITDTALAQGWRPDMSKVTPRKERVAIVGAGPAGLGC
ADILARNGIKAVVFDKYPQIGGLLTYGIPSFKLDKSVMQTRRTVLEGMGIEFRLGVTVGKDIAFADLLKDYDAVFLGMGT
YSAMKAGLPGEDADGVLQALPYLIGNTMSLLGQQSEQNPYIDLAGKKVVVLGGGDTAMDCVRTAVRQGAREVTCAYRRDE
ANMPGSRREVQNAREEGVNFLFNRQPVAIKTENGAVVGIECIETRMGEPDANGRRSAEPVPGSEQLLEADAIIIAFGFQP
SPAPWFADFDIALDDKGRVKASKADDNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGREAALGILNYLESAKA
>Mature_471_residues
SNDFQFIQVGRKDPTKQPIKLRATQFIEIYQPFTQPQVNEQADRCLDCGNPYCEWKCPLHNYIPNWLKLAKQGRIMEAAD
LVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGNVEKYITDTALAQGWRPDMSKVTPRKERVAIVGAGPAGLGCA
DILARNGIKAVVFDKYPQIGGLLTYGIPSFKLDKSVMQTRRTVLEGMGIEFRLGVTVGKDIAFADLLKDYDAVFLGMGTY
SAMKAGLPGEDADGVLQALPYLIGNTMSLLGQQSEQNPYIDLAGKKVVVLGGGDTAMDCVRTAVRQGAREVTCAYRRDEA
NMPGSRREVQNAREEGVNFLFNRQPVAIKTENGAVVGIECIETRMGEPDANGRRSAEPVPGSEQLLEADAIIIAFGFQPS
PAPWFADFDIALDDKGRVKASKADDNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGREAALGILNYLESAKA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=454, Percent_Identity=29.295154185022, Blast_Score=169, Evalue=6e-42,
Organism=Escherichia coli, GI1789606, Length=468, Percent_Identity=66.025641025641, Blast_Score=672, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=460, Percent_Identity=60.4347826086956, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI87082180, Length=461, Percent_Identity=56.6160520607375, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1788468, Length=445, Percent_Identity=29.6629213483146, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789243, Length=426, Percent_Identity=30.2816901408451, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17570289, Length=488, Percent_Identity=32.7868852459016, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71984108, Length=454, Percent_Identity=29.9559471365639, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17543860, Length=162, Percent_Identity=33.9506172839506, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=480, Percent_Identity=32.5, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=37.319587628866, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=37.319587628866, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24665547, Length=484, Percent_Identity=37.8099173553719, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24665543, Length=484, Percent_Identity=37.8099173553719, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24640763, Length=477, Percent_Identity=28.3018867924528, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI18858217, Length=477, Percent_Identity=28.3018867924528, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51338; Mature: 51207

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDFQFIQVGRKDPTKQPIKLRATQFIEIYQPFTQPQVNEQADRCLDCGNPYCEWKCPL
CCCCCEEEEECCCCCCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEECCH
HNYIPNWLKLAKQGRIMEAADLVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGN
HHHCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCEEEECC
VEKYITDTALAQGWRPDMSKVTPRKERVAIVGAGPAGLGCADILARNGIKAVVFDKYPQI
HHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEECCCCC
GGLLTYGIPSFKLDKSVMQTRRTVLEGMGIEFRLGVTVGKDIAFADLLKDYDAVFLGMGT
CCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCEEEEECCH
YSAMKAGLPGEDADGVLQALPYLIGNTMSLLGQQSEQNPYIDLAGKKVVVLGGGDTAMDC
HHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCEEEECCCEEEEECCCCHHHHH
VRTAVRQGAREVTCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPVAIKTENGAVVGIE
HHHHHHCCCHHEEHHEECCCCCCCCCHHHHHHHHHHCCCEEECCCCEEEEECCCCEEEEE
CIETRMGEPDANGRRSAEPVPGSEQLLEADAIIIAFGFQPSPAPWFADFDIALDDKGRVK
EHHHHCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCEEEEEEEECCCCCEE
ASKADDNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGREAALGILNYLESAKA
ECCCCCCCCCCCCCEEEECCCEECCCCEEEEEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNDFQFIQVGRKDPTKQPIKLRATQFIEIYQPFTQPQVNEQADRCLDCGNPYCEWKCPL
CCCCEEEEECCCCCCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEECCH
HNYIPNWLKLAKQGRIMEAADLVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGN
HHHCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCEEEECC
VEKYITDTALAQGWRPDMSKVTPRKERVAIVGAGPAGLGCADILARNGIKAVVFDKYPQI
HHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEECCCCC
GGLLTYGIPSFKLDKSVMQTRRTVLEGMGIEFRLGVTVGKDIAFADLLKDYDAVFLGMGT
CCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCEEEEECCH
YSAMKAGLPGEDADGVLQALPYLIGNTMSLLGQQSEQNPYIDLAGKKVVVLGGGDTAMDC
HHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCEEEECCCEEEEECCCCHHHHH
VRTAVRQGAREVTCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPVAIKTENGAVVGIE
HHHHHHCCCHHEEHHEECCCCCCCCCHHHHHHHHHHCCCEEECCCCEEEEECCCCEEEEE
CIETRMGEPDANGRRSAEPVPGSEQLLEADAIIIAFGFQPSPAPWFADFDIALDDKGRVK
EHHHHCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCEEEEEEEECCCCCEE
ASKADDNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGREAALGILNYLESAKA
ECCCCCCCCCCCCCEEEECCCEECCCCEEEEEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]